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Protein

Cyclin-dependent kinase 4 inhibitor B

Gene

Cdkn2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest (By similarity).By similarity

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase inhibitor activity Source: MGI
  2. protein kinase binding Source: MGI

GO - Biological processi

  1. aging Source: Ensembl
  2. cell cycle arrest Source: MGI
  3. cellular response to extracellular stimulus Source: MGI
  4. cellular response to nutrient Source: MGI
  5. G2/M transition of mitotic cell cycle Source: MGI
  6. liver development Source: Ensembl
  7. megakaryocyte differentiation Source: Ensembl
  8. mitotic cell cycle checkpoint Source: MGI
  9. negative regulation of cell proliferation Source: MGI
  10. negative regulation of epithelial cell proliferation Source: MGI
  11. negative regulation of G1/S transition of mitotic cell cycle Source: MGI
  12. negative regulation of phosphorylation Source: MGI
  13. negative regulation of protein serine/threonine kinase activity Source: MGI
  14. positive regulation of epithelial cell differentiation Source: Ensembl
  15. positive regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  16. regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
  17. response to cytokine Source: Ensembl
  18. response to organic cyclic compound Source: Ensembl
  19. spleen development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Enzyme and pathway databases

ReactomeiREACT_289448. Cyclin D associated events in G1.
REACT_305463. Oxidative Stress Induced Senescence.
REACT_339940. Oncogene Induced Senescence.
REACT_350665. Senescence-Associated Secretory Phenotype (SASP).

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 4 inhibitor B
Alternative name(s):
p14-INK4b
p15-INK4b
Gene namesi
Name:Cdkn2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:104737. Cdkn2b.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleoplasm Source: MGI
  3. nucleus Source: MGI
Complete GO annotation...

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 130130Cyclin-dependent kinase 4 inhibitor BPRO_0000144185Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP55271.
PRIDEiP55271.

PTM databases

PhosphoSiteiP55271.

Expressioni

Tissue specificityi

Expressed ubiquitously.

Inductioni

By TGF-beta.

Gene expression databases

BgeeiP55271.
ExpressionAtlasiP55271. baseline and differential.
GenevestigatoriP55271.

Interactioni

Subunit structurei

Heterodimer of CDKN2B with CDK4 or CDK6.By similarity

Protein-protein interaction databases

DIPiDIP-60249N.

Structurei

Secondary structure

1
130
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 157Combined sources
Helixi19 – 279Combined sources
Turni41 – 455Combined sources
Helixi51 – 6010Combined sources
Turni69 – 724Combined sources
Helixi75 – 828Combined sources
Helixi85 – 939Combined sources
Beta strandi102 – 1065Combined sources
Helixi108 – 1158Combined sources
Helixi118 – 12811Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D9SNMR-A1-130[»]
ProteinModelPortaliP55271.
SMRiP55271. Positions 1-130.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55271.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati5 – 3430ANK 1Add
BLAST
Repeati38 – 6629ANK 2Add
BLAST
Repeati71 – 10030ANK 3Add
BLAST
Repeati104 – 13027ANK 4Add
BLAST

Sequence similaritiesi

Contains 4 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiNOG287192.
HOGENOMiHOG000290191.
HOVERGENiHBG050870.
InParanoidiP55271.
KOiK04685.
OMAiVMMMGSV.
OrthoDBiEOG7TTQ94.
PhylomeDBiP55271.
TreeFamiTF352389.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55271-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGGSSDAGL ATAAARGQVE TVRQLLEAGA DPNALNRFGR RPIQVMMMGS
60 70 80 90 100
AQVAELLLLH GAEPNCADPA TLTRPVHDAA REGFLDTLVV LHRAGARLDV
110 120 130
CDAWGRLPVD LAEEQGHRDI ARYLHAATGD
Length:130
Mass (Da):13,789
Last modified:October 1, 1996 - v1
Checksum:i7AAD60FF552BCFF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66085, U66084 Genomic DNA. Translation: AAB39833.1.
BC002010 mRNA. Translation: AAH02010.1.
CCDSiCCDS18351.1.
RefSeqiNP_031696.1. NM_007670.4.
UniGeneiMm.423094.

Genome annotation databases

EnsembliENSMUST00000097981; ENSMUSP00000095595; ENSMUSG00000073802.
GeneIDi12579.
KEGGimmu:12579.
UCSCiuc008tok.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66085, U66084 Genomic DNA. Translation: AAB39833.1.
BC002010 mRNA. Translation: AAH02010.1.
CCDSiCCDS18351.1.
RefSeqiNP_031696.1. NM_007670.4.
UniGeneiMm.423094.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D9SNMR-A1-130[»]
ProteinModelPortaliP55271.
SMRiP55271. Positions 1-130.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60249N.

PTM databases

PhosphoSiteiP55271.

Proteomic databases

MaxQBiP55271.
PRIDEiP55271.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097981; ENSMUSP00000095595; ENSMUSG00000073802.
GeneIDi12579.
KEGGimmu:12579.
UCSCiuc008tok.2. mouse.

Organism-specific databases

CTDi1030.
MGIiMGI:104737. Cdkn2b.

Phylogenomic databases

eggNOGiNOG287192.
HOGENOMiHOG000290191.
HOVERGENiHBG050870.
InParanoidiP55271.
KOiK04685.
OMAiVMMMGSV.
OrthoDBiEOG7TTQ94.
PhylomeDBiP55271.
TreeFamiTF352389.

Enzyme and pathway databases

ReactomeiREACT_289448. Cyclin D associated events in G1.
REACT_305463. Oxidative Stress Induced Senescence.
REACT_339940. Oncogene Induced Senescence.
REACT_350665. Senescence-Associated Secretory Phenotype (SASP).

Miscellaneous databases

EvolutionaryTraceiP55271.
NextBioi281704.
PROiP55271.
SOURCEiSearch...

Gene expression databases

BgeeiP55271.
ExpressionAtlasiP55271. baseline and differential.
GenevestigatoriP55271.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of murine p16INK4a and p15INK4b genes."
    Quelle D.E., Ashmun R.A., Hannon G.J., Rehberger P.A., Trono D., Richter K.H., Walker C., Beach D., Sherr C.J., Serrano M.
    Oncogene 11:635-645(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Inactivation of the cyclin-dependent kinase inhibitor p15INK4b by deletion and de novo methylation with independence of p16INK4a alterations in murine primary T-cell lymphomas."
    Malumbres M., de Castro I.P., Santos J., Melendez B., Mangues R., Serrano M., Pellicer A., Fernandez-Piqueras J.
    Oncogene 14:1361-1370(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6J X DBA.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-12, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  5. "Tumor suppressor INK4: comparisons of conformational properties between p16(INK4A) and p18(INK4C)."
    Yuan C., Li J., Selby T.L., Byeon I.-J., Tsai M.-D.
    J. Mol. Biol. 294:201-211(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiCDN2B_MOUSE
AccessioniPrimary (citable) accession number: P55271
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 1, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.