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Protein

Adenosine kinase

Gene

Adk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.

Catalytic activityi

ATP + adenosine = ADP + AMP.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Activity is inhibited by 5-iodotubercidin and 5'-amino-5'-deoxyadenosine.By similarity

Pathwayi: AMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from adenosine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosine kinase (Adk)
This subpathway is part of the pathway AMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from adenosine, the pathway AMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi147Magnesium 2By similarity1
Active sitei316By similarity1

GO - Molecular functioni

GO - Biological processi

  • adenosine salvage Source: GO_Central
  • AMP salvage Source: UniProtKB-UniPathway
  • dATP biosynthetic process Source: MGI
  • purine ribonucleoside salvage Source: MGI

Keywordsi

Molecular functionKinase, Transferase
Biological processPurine salvage
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.20. 3474.
ReactomeiR-MMU-74217. Purine salvage.
UniPathwayiUPA00588; UER00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine kinase (EC:2.7.1.20)
Short name:
AK
Alternative name(s):
Adenosine 5'-phosphotransferase
Gene namesi
Name:Adk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:87930. Adk.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi48S → A: No effect. 1 Publication1
Mutagenesisi85S → A: No effect. 1 Publication1
Mutagenesisi272S → A: No effect. 1 Publication1
Mutagenesisi328S → A: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000800541 – 361Adenosine kinaseAdd BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei76PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP55264.
MaxQBiP55264.
PaxDbiP55264.
PeptideAtlasiP55264.
PRIDEiP55264.

2D gel databases

REPRODUCTION-2DPAGEiP55264.

PTM databases

iPTMnetiP55264.
PhosphoSitePlusiP55264.
SwissPalmiP55264.

Expressioni

Tissue specificityi

Highest expression in liver, testis, kidney and spleen. Lower expression in brain. Isoform Long is predominant in testis and kidney and isoform Short is predominant in the spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000039197.
CleanExiMM_ADK.
GenevisibleiP55264. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiP55264. 3 interactors.
MINTiMINT-1854964.
STRINGi10090.ENSMUSP00000047665.

Structurei

Secondary structure

1361
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi25 – 29Combined sources5
Beta strandi32 – 38Combined sources7
Helixi41 – 46Combined sources6
Beta strandi54 – 56Combined sources3
Helixi59 – 61Combined sources3
Helixi64 – 71Combined sources8
Beta strandi75 – 80Combined sources6
Helixi81 – 93Combined sources13
Beta strandi100 – 109Combined sources10
Helixi110 – 121Combined sources12
Beta strandi125 – 134Combined sources10
Beta strandi138 – 144Combined sources7
Beta strandi147 – 153Combined sources7
Helixi155 – 159Combined sources5
Helixi162 – 164Combined sources3
Turni165 – 167Combined sources3
Helixi169 – 176Combined sources8
Beta strandi179 – 184Combined sources6
Helixi185 – 189Combined sources5
Helixi192 – 204Combined sources13
Beta strandi208 – 212Combined sources5
Helixi216 – 221Combined sources6
Helixi223 – 229Combined sources7
Helixi230 – 232Combined sources3
Beta strandi234 – 239Combined sources6
Helixi240 – 249Combined sources10
Helixi257 – 265Combined sources9
Beta strandi277 – 282Combined sources6
Beta strandi285 – 290Combined sources6
Beta strandi295 – 298Combined sources4
Helixi305 – 307Combined sources3
Helixi314 – 326Combined sources13
Turni327 – 329Combined sources3
Helixi332 – 346Combined sources15
Beta strandi349 – 352Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5KB5X-ray1.80A20-361[»]
5KB6X-ray1.20A/B1-361[»]
ProteinModelPortaliP55264.
SMRiP55264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi7 – 15Nuclear localization signalBy similarity9

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family.Curated

Phylogenomic databases

eggNOGiKOG2854. Eukaryota.
COG0524. LUCA.
GeneTreeiENSGT00390000014320.
HOGENOMiHOG000172419.
HOVERGENiHBG002367.
InParanoidiP55264.
KOiK00856.
OMAiVNPPSIM.
OrthoDBiEOG091G0JQC.
PhylomeDBiP55264.
TreeFamiTF300745.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiView protein in InterPro
IPR001805. Adenokinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
PfamiView protein in Pfam
PF00294. PfkB. 1 hit.
PRINTSiPR00989. ADENOKINASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiView protein in PROSITE
PS00584. PFKB_KINASES_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P55264-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAADEPKPK KLKVEAPQAL SENVLFGMGN PLLDISAVVD KDFLDKYSLK
60 70 80 90 100
PNDQILAEDK HKELFDELVK KFKVEYHAGG STQNSMKVAQ WLIQEPHKAA
110 120 130 140 150
TFFGCIGIDK FGEILKRKAA DAHVDAHYYE QNEQPTGTCA ACITGGNRSL
160 170 180 190 200
VANLAAANCY KKEKHLDLER NWVLVEKARV YYIAGFFLTV SPESVLKVAR
210 220 230 240 250
YAAENNRVFT LNLSAPFISQ FFKEALMDVM PYVDILFGNE TEAATFAREQ
260 270 280 290 300
GFETKDIKEI AKKAQALPKV NSKRQRTVIF TQGRDDTIVA AENDVTAFPV
310 320 330 340 350
LDQNQEEIID TNGAGDAFVG GFLSQLVSDK PLTECIRAGH YAASVIIRRT
360
GCTFPEKPDF H
Length:361
Mass (Da):40,149
Last modified:July 19, 2005 - v2
Checksum:i73D5D0451B606757
GO
Isoform Short (identifier: P55264-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MAAADEPKPKKLKVEAPQAL → MTST

Show »
Length:345
Mass (Da):38,452
Checksum:iBFE9B79EF1523D87
GO

Sequence cautioni

The sequence AAA91649 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92L → M in AAA91649 (PubMed:8917457).Curated1
Sequence conflicti98K → R in AAA91649 (PubMed:8917457).Curated1
Sequence conflicti117R → S in AAA91649 (PubMed:8917457).Curated1
Sequence conflicti155A → R in AAA91649 (PubMed:8917457).Curated1
Sequence conflicti170R → N in AAA91649 (PubMed:8917457).Curated1
Sequence conflicti173V → M in AAA91649 (PubMed:8917457).Curated1
Sequence conflicti208V → T in AAA91649 (PubMed:8917457).Curated1
Sequence conflicti228D → A in AAA91649 (PubMed:8917457).Curated1
Sequence conflicti256D → G in AAT07066 (PubMed:15317590).Curated1
Sequence conflicti262 – 264KKA → RKT in AAA91649 (PubMed:8917457).Curated3
Sequence conflicti281T → RN in AAA91649 (PubMed:8917457).Curated1
Sequence conflicti291 – 292AE → TG in AAA91649 (PubMed:8917457).Curated2
Sequence conflicti303Q → E in AAA91649 (PubMed:8917457).Curated1
Sequence conflicti309I → V in AAA91649 (PubMed:8917457).Curated1
Sequence conflicti314A → V in AAT07066 (PubMed:15317590).Curated1
Sequence conflicti329D → N in AAA91649 (PubMed:8917457).Curated1
Sequence conflicti359D → N in AAA91649 (PubMed:8917457).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0147561 – 20MAAAD…APQAL → MTST in isoform Short. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY540996 mRNA. Translation: AAT07065.1.
AY540997 mRNA. Translation: AAT07066.1.
BC009659 mRNA. Translation: AAH09659.1.
U26589 mRNA. Translation: AAA91649.1. Different initiation.
CCDSiCCDS26862.1. [P55264-1]
RefSeqiNP_001229970.1. NM_001243041.1. [P55264-2]
NP_598840.1. NM_134079.4. [P55264-1]
UniGeneiMm.188734.

Genome annotation databases

EnsembliENSMUST00000045376; ENSMUSP00000047665; ENSMUSG00000039197. [P55264-1]
ENSMUST00000224069; ENSMUSP00000153089; ENSMUSG00000039197. [P55264-2]
GeneIDi11534.
KEGGimmu:11534.
UCSCiuc007sld.2. mouse. [P55264-1]
uc007sle.2. mouse. [P55264-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiADK_MOUSE
AccessioniPrimary (citable) accession number: P55264
Secondary accession number(s): Q6JAM3, Q91VJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 19, 2005
Last modified: November 22, 2017
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families