Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P55264 (ADK_MOUSE)

Last modified January 19, 2010. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Adenosine kinase
      Short name=AK
    EC=2.7.1.20
Alternative name(s):
    Adenosine 5'-phosphotransferase
Gene names
Name: Adk
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Hide | Top

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.

Catalytic activity

ATP + adenosine = ADP + AMP.

Cofactor

Binds 3 magnesium ions per subunit By similarity.

Enzyme regulation

Activity is inhibited by 5-iodotubercidin and 5'-amino-5'-deoxyadenosine By similarity.

Pathway

Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1.

Subunit structure

Monomer By similarity.

Tissue specificity

Highest expression in liver, testis, kidney and spleen. Lower expression in brain. Isoform Long is predominant in testis and kidney and isoform Short is predominant in the spleen. Ref.1

Sequence similarities

Belongs to the carbohydrate kinase pfkB family.

Hide | Top

Ontologies

Keywords
   Biological processPurine salvage
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMAcetylation
Gene Ontology (GO)
   Biological processdATP biosynthetic process

Inferred from mutant phenotype. Source: MGI

purine ribonucleoside salvage

Inferred from direct assay. Source: MGI

   Cellular componentcytosol

Inferred from direct assay. Source: MGI

nucleus

Inferred from direct assay. Source: MGI

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

adenosine kinase activity

Inferred from direct assay. Source: MGI

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Hide | Top

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P55264-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P55264-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MAAADEPKPKKLKVEAPQAL → MTST

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 361360Adenosine kinase
PRO_0000080054

Sites

Active site3161 By similarity
Metal binding1471Magnesium 2 By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Natural variations

Alternative sequence1 – 2020MAAAD…APQAL → MTST in isoform Short.
VSP_014756

Experimental info

Mutagenesis481S → A: No effect. Ref.1
Mutagenesis851S → A: No effect. Ref.1
Mutagenesis2721S → A: No effect. Ref.1
Mutagenesis3281S → A: No effect. Ref.1
Sequence conflict921L → M in AAA91649. Ref.3
Sequence conflict981K → R in AAA91649. Ref.3
Sequence conflict1171R → S in AAA91649. Ref.3
Sequence conflict1551A → R in AAA91649. Ref.3
Sequence conflict1701R → N in AAA91649. Ref.3
Sequence conflict1731V → M in AAA91649. Ref.3
Sequence conflict2081V → T in AAA91649. Ref.3
Sequence conflict2281D → A in AAA91649. Ref.3
Sequence conflict2561D → G in AAT07066. Ref.1
Sequence conflict262 – 2643KKA → RKT in AAA91649. Ref.3
Sequence conflict2811T → RN in AAA91649. Ref.3
Sequence conflict291 – 2922AE → TG in AAA91649. Ref.3
Sequence conflict3031Q → E in AAA91649. Ref.3
Sequence conflict3091I → V in AAA91649. Ref.3
Sequence conflict3141A → V in AAT07066. Ref.1
Sequence conflict3291D → N in AAA91649. Ref.3
Sequence conflict3591D → N in AAA91649. Ref.3

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified July 19, 2005. Version 2.
Checksum: 73D5D0451B606757

FASTA36140,149
        10         20         30         40         50         60 
MAAADEPKPK KLKVEAPQAL SENVLFGMGN PLLDISAVVD KDFLDKYSLK PNDQILAEDK 

        70         80         90        100        110        120 
HKELFDELVK KFKVEYHAGG STQNSMKVAQ WLIQEPHKAA TFFGCIGIDK FGEILKRKAA 

       130        140        150        160        170        180 
DAHVDAHYYE QNEQPTGTCA ACITGGNRSL VANLAAANCY KKEKHLDLER NWVLVEKARV 

       190        200        210        220        230        240 
YYIAGFFLTV SPESVLKVAR YAAENNRVFT LNLSAPFISQ FFKEALMDVM PYVDILFGNE 

       250        260        270        280        290        300 
TEAATFAREQ GFETKDIKEI AKKAQALPKV NSKRQRTVIF TQGRDDTIVA AENDVTAFPV 

       310        320        330        340        350        360 
LDQNQEEIID TNGAGDAFVG GFLSQLVSDK PLTECIRAGH YAASVIIRRT GCTFPEKPDF 


H

« Hide

Isoform Short.

Checksum: BFE9B79EF1523D87
Show »

FASTA34538,452

Hide | Top

References

« Hide 'large scale' references
[1]"Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation."
Sahin B., Kansy J.W., Nairn A.C., Spychala J., Ealick S.E., Fienberg A.A., Greene R.W., Bibb J.A.
Eur. J. Biochem. 271:3547-3555(2004) [PubMed: 15317590] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), TISSUE SPECIFICITY, MUTAGENESIS OF SER-48; SER-85; SER-272 AND SER-328.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Strain: FVB/N.
Tissue: Mammary tumor.
[3]"Cloning and characterization of cDNA for adenosine kinase from mammalian (Chinese hamster, mouse, human and rat) species. High frequency mutants of Chinese hamster ovary cells involve structural alterations in the gene."
Singh B., Hao W., Wu Z.-C., Eigl B., Gupta R.S.
Eur. J. Biochem. 241:564-571(1996) [PubMed: 8917457] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 84-361, CHARACTERIZATION.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY540996 mRNA. Translation: AAT07065.1.
AY540997 mRNA. Translation: AAT07066.1.
BC009659 mRNA. Translation: AAH09659.1.
U26589 mRNA. Translation: AAA91649.1. Different initiation.
IPIIPI00126940.
IPI00138084.
RefSeqNP_598840.1.
UniGeneMm.188734

3D structure databases

SMRP55264. Positions 22-361.
ModBaseSearch...

Protein-protein interaction databases

STRINGP55264.

2-D gel databases

REPRODUCTION-2DPAGEP55264.

Proteomic databases

PRIDEP55264.

Genome annotation databases

EnsemblENSMUST00000045376; ENSMUSP00000047665; ENSMUSG00000039197; Mus musculus. [Genome view]
GeneID11534.
KEGGmmu:11534.
NMPDRfig|10090.3.peg.28647.
UCSCuc007sld.1. mouse.
uc007sle.1. mouse.

Organism-specific databases

CTD11534.
MGIMGI:87930. Adk.

Phylogenomic databases

eggNOGroNOG07404.
HOGENOMHBG314716.
HOVERGENP55264.
InParanoidP55264.
OMAAQWMIQQ.
OrthoDBEOG9K6JQT.
PhylomeDBP55264.

Enzyme and pathway databases

BRENDA2.7.1.20. 244.

Gene expression databases

ArrayExpressP55264.
BgeeP55264.
CleanExMM_ADK.
GenevestigatorP55264.
GermOnlineENSMUSG00000039197. Mus musculus.

Family and domain databases

InterProIPR001805. Adenokinase.
IPR011611. Carb/pur_kinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
[Graphical view]
PANTHERPTHR10584:SF24. Adenokinase. 1 hit.
PfamPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSPR00989. ADENOKINASE.
PROSITEPS00583. PFKB_KINASES_1. False negative.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio278974.
SOURCESearch...

Hide | Top

Entry information

Entry nameADK_MOUSE
AccessionPrimary (citable) accession number: P55264
Secondary accession number(s): Q6JAM3, Q91VJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 19, 2005
Last modified: January 19, 2010
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents