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Protein

Adenosine kinase

Gene

Adk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.

Catalytic activityi

ATP + adenosine = ADP + AMP.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Activity is inhibited by 5-iodotubercidin and 5'-amino-5'-deoxyadenosine.By similarity

Pathwayi: AMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from adenosine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosine kinase (Adk)
This subpathway is part of the pathway AMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from adenosine, the pathway AMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi147 – 1471Magnesium 2By similarity
Active sitei316 – 3161By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.20. 3474.
ReactomeiR-MMU-74217. Purine salvage.
UniPathwayiUPA00588; UER00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine kinase (EC:2.7.1.20)
Short name:
AK
Alternative name(s):
Adenosine 5'-phosphotransferase
Gene namesi
Name:Adk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:87930. Adk.

Subcellular locationi

Isoform Long :
  • Nucleus By similarity
Isoform Short :
  • Cytoplasm By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi48 – 481S → A: No effect. 1 Publication
Mutagenesisi85 – 851S → A: No effect. 1 Publication
Mutagenesisi272 – 2721S → A: No effect. 1 Publication
Mutagenesisi328 – 3281S → A: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Adenosine kinasePRO_0000080054Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei76 – 761PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP55264.
MaxQBiP55264.
PaxDbiP55264.
PeptideAtlasiP55264.
PRIDEiP55264.

2D gel databases

REPRODUCTION-2DPAGEP55264.

PTM databases

iPTMnetiP55264.
PhosphoSiteiP55264.
SwissPalmiP55264.

Expressioni

Tissue specificityi

Highest expression in liver, testis, kidney and spleen. Lower expression in brain. Isoform Long is predominant in testis and kidney and isoform Short is predominant in the spleen.1 Publication

Gene expression databases

BgeeiP55264.
CleanExiMM_ADK.
GenevisibleiP55264. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiP55264. 3 interactions.
MINTiMINT-1854964.
STRINGi10090.ENSMUSP00000047665.

Structurei

3D structure databases

ProteinModelPortaliP55264.
SMRiP55264. Positions 22-361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi7 – 159Nuclear localization signalBy similarity

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family.Curated

Phylogenomic databases

eggNOGiKOG2854. Eukaryota.
COG0524. LUCA.
GeneTreeiENSGT00390000014320.
HOGENOMiHOG000172419.
HOVERGENiHBG002367.
InParanoidiP55264.
KOiK00856.
OMAiISQFYMA.
OrthoDBiEOG7VMP5W.
PhylomeDBiP55264.
TreeFamiTF300745.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR001805. Adenokinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10584:SF24. PTHR10584:SF24. 1 hit.
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSiPR00989. ADENOKINASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P55264-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAADEPKPK KLKVEAPQAL SENVLFGMGN PLLDISAVVD KDFLDKYSLK
60 70 80 90 100
PNDQILAEDK HKELFDELVK KFKVEYHAGG STQNSMKVAQ WLIQEPHKAA
110 120 130 140 150
TFFGCIGIDK FGEILKRKAA DAHVDAHYYE QNEQPTGTCA ACITGGNRSL
160 170 180 190 200
VANLAAANCY KKEKHLDLER NWVLVEKARV YYIAGFFLTV SPESVLKVAR
210 220 230 240 250
YAAENNRVFT LNLSAPFISQ FFKEALMDVM PYVDILFGNE TEAATFAREQ
260 270 280 290 300
GFETKDIKEI AKKAQALPKV NSKRQRTVIF TQGRDDTIVA AENDVTAFPV
310 320 330 340 350
LDQNQEEIID TNGAGDAFVG GFLSQLVSDK PLTECIRAGH YAASVIIRRT
360
GCTFPEKPDF H
Length:361
Mass (Da):40,149
Last modified:July 19, 2005 - v2
Checksum:i73D5D0451B606757
GO
Isoform Short (identifier: P55264-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MAAADEPKPKKLKVEAPQAL → MTST

Show »
Length:345
Mass (Da):38,452
Checksum:iBFE9B79EF1523D87
GO

Sequence cautioni

The sequence AAA91649.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 921L → M in AAA91649 (PubMed:8917457).Curated
Sequence conflicti98 – 981K → R in AAA91649 (PubMed:8917457).Curated
Sequence conflicti117 – 1171R → S in AAA91649 (PubMed:8917457).Curated
Sequence conflicti155 – 1551A → R in AAA91649 (PubMed:8917457).Curated
Sequence conflicti170 – 1701R → N in AAA91649 (PubMed:8917457).Curated
Sequence conflicti173 – 1731V → M in AAA91649 (PubMed:8917457).Curated
Sequence conflicti208 – 2081V → T in AAA91649 (PubMed:8917457).Curated
Sequence conflicti228 – 2281D → A in AAA91649 (PubMed:8917457).Curated
Sequence conflicti256 – 2561D → G in AAT07066 (PubMed:15317590).Curated
Sequence conflicti262 – 2643KKA → RKT in AAA91649 (PubMed:8917457).Curated
Sequence conflicti281 – 2811T → RN in AAA91649 (PubMed:8917457).Curated
Sequence conflicti291 – 2922AE → TG in AAA91649 (PubMed:8917457).Curated
Sequence conflicti303 – 3031Q → E in AAA91649 (PubMed:8917457).Curated
Sequence conflicti309 – 3091I → V in AAA91649 (PubMed:8917457).Curated
Sequence conflicti314 – 3141A → V in AAT07066 (PubMed:15317590).Curated
Sequence conflicti329 – 3291D → N in AAA91649 (PubMed:8917457).Curated
Sequence conflicti359 – 3591D → N in AAA91649 (PubMed:8917457).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2020MAAAD…APQAL → MTST in isoform Short. 1 PublicationVSP_014756Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY540996 mRNA. Translation: AAT07065.1.
AY540997 mRNA. Translation: AAT07066.1.
BC009659 mRNA. Translation: AAH09659.1.
U26589 mRNA. Translation: AAA91649.1. Different initiation.
CCDSiCCDS26862.1. [P55264-1]
RefSeqiNP_001229970.1. NM_001243041.1. [P55264-2]
NP_598840.1. NM_134079.4. [P55264-1]
UniGeneiMm.188734.

Genome annotation databases

EnsembliENSMUST00000045376; ENSMUSP00000047665; ENSMUSG00000039197. [P55264-1]
GeneIDi11534.
KEGGimmu:11534.
UCSCiuc007sld.2. mouse. [P55264-1]
uc007sle.2. mouse. [P55264-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY540996 mRNA. Translation: AAT07065.1.
AY540997 mRNA. Translation: AAT07066.1.
BC009659 mRNA. Translation: AAH09659.1.
U26589 mRNA. Translation: AAA91649.1. Different initiation.
CCDSiCCDS26862.1. [P55264-1]
RefSeqiNP_001229970.1. NM_001243041.1. [P55264-2]
NP_598840.1. NM_134079.4. [P55264-1]
UniGeneiMm.188734.

3D structure databases

ProteinModelPortaliP55264.
SMRiP55264. Positions 22-361.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP55264. 3 interactions.
MINTiMINT-1854964.
STRINGi10090.ENSMUSP00000047665.

PTM databases

iPTMnetiP55264.
PhosphoSiteiP55264.
SwissPalmiP55264.

2D gel databases

REPRODUCTION-2DPAGEP55264.

Proteomic databases

EPDiP55264.
MaxQBiP55264.
PaxDbiP55264.
PeptideAtlasiP55264.
PRIDEiP55264.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045376; ENSMUSP00000047665; ENSMUSG00000039197. [P55264-1]
GeneIDi11534.
KEGGimmu:11534.
UCSCiuc007sld.2. mouse. [P55264-1]
uc007sle.2. mouse. [P55264-2]

Organism-specific databases

CTDi132.
MGIiMGI:87930. Adk.

Phylogenomic databases

eggNOGiKOG2854. Eukaryota.
COG0524. LUCA.
GeneTreeiENSGT00390000014320.
HOGENOMiHOG000172419.
HOVERGENiHBG002367.
InParanoidiP55264.
KOiK00856.
OMAiISQFYMA.
OrthoDBiEOG7VMP5W.
PhylomeDBiP55264.
TreeFamiTF300745.

Enzyme and pathway databases

UniPathwayiUPA00588; UER00659.
BRENDAi2.7.1.20. 3474.
ReactomeiR-MMU-74217. Purine salvage.

Miscellaneous databases

ChiTaRSiAdk. mouse.
PROiP55264.
SOURCEiSearch...

Gene expression databases

BgeeiP55264.
CleanExiMM_ADK.
GenevisibleiP55264. MM.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR001805. Adenokinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10584:SF24. PTHR10584:SF24. 1 hit.
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSiPR00989. ADENOKINASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation."
    Sahin B., Kansy J.W., Nairn A.C., Spychala J., Ealick S.E., Fienberg A.A., Greene R.W., Bibb J.A.
    Eur. J. Biochem. 271:3547-3555(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), TISSUE SPECIFICITY, MUTAGENESIS OF SER-48; SER-85; SER-272 AND SER-328.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "Cloning and characterization of cDNA for adenosine kinase from mammalian (Chinese hamster, mouse, human and rat) species. High frequency mutants of Chinese hamster ovary cells involve structural alterations in the gene."
    Singh B., Hao W., Wu Z.-C., Eigl B., Gupta R.S.
    Eur. J. Biochem. 241:564-571(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 84-361, CHARACTERIZATION.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiADK_MOUSE
AccessioniPrimary (citable) accession number: P55264
Secondary accession number(s): Q6JAM3, Q91VJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 19, 2005
Last modified: July 6, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.