Reviewed, UniProtKB/Swiss-Prot P55262 (ADK_CRIGR)

Names and origin

Protein names

Recommended name:
    Adenosine kinase
      Short name=AK
    EC=2.7.1.20
Alternative name(s):
    Adenosine 5'-phosphotransferase

Gene names

Name:

ADK

Organism

Cricetulus griseus (Chinese hamster)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Catalytic activity	ATP + adenosine = ADP + AMP.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Enzyme regulation	Activity is inhibited by 5-iodotubercidin and 5'-amino-5'-deoxyadenosine By similarity.
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1.
Subunit structure	Monomer By similarity.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.
Sequence caution	The sequence AAA91648.1 differs from that shown. Reason: Frameshift at position 16.

Ontologies

Keywords
Biological process	Purine salvage
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenosine kinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Adenosine kinase		PRO_0000080052
Sites
	Active site	316	1	By similarity
	Metal binding	48	1	Magnesium 1 By similarity
	Metal binding	146	1	Magnesium 2 By similarity
	Metal binding	147	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55262-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 9109AADD81DF86E6	FASTA	361	40,286
	10 20 30 40 50 60 MAAAEEPKPK KLKVEXPQAL SENVLFGMGN PLLDISAVVD KDFLDKYSLK PNDQILAEEK 70 80 90 100 110 120 HKELFDELVR KFKVEYHAGG STQNSIKVAQ WMIQKPHKAA TFFGCIGIDK FGEILKSKAA 130 140 150 160 170 180 EAHVDAHYYE QNEQPTGTCA ACITGDNRSL VANLAAANCY KKEKHLDLEN NWVLVEKARV 190 200 210 220 230 240 YYIAGFFLTV SPESVLKVAR YAAENNRIFT LNLSAPFISQ FFKESLMEVM PYVDILFGNE 250 260 270 280 290 300 TEAATFAREQ GFETKDIKEI AKKAQALAKV NSKRPRTVVF TQGRDDTVVA TENEVMAFAV 310 320 330 340 350 360 LDQNQKEIID TNGAGDAFVG GFLSQLVYNK PLTECIRAGH YAASVIIRRT GCTFPEKPDF H

References

[1]

"Cloning and characterization of cDNA for adenosine kinase from mammalian (Chinese hamster, mouse, human and rat) species. High frequency mutants of Chinese hamster ovary cells involve structural alterations in the gene." Singh B., Hao W., Wu Z.-C., Eigl B., Gupta R.S. Eur. J. Biochem. 241:564-571(1996) [PubMed: 8917457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION. Tissue: Liver and Ovary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U26588 mRNA. Translation: AAA91648.1. Frameshift.
PIR	JC7368.
3D structure databases
HSSP	HSSP built from PDB template 1BX4 based on UniProtKB P55263.
SMR	P55262. Positions 22-361.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P55262.
Enzyme and pathway databases
BRENDA	2.7.1.20. 18.
Family and domain databases
InterPro	IPR001805. Adenokinase. IPR011611. Carb/pur_kinase. IPR002173. Carboh/pur_kinase_PfkB_CS. [Graphical view]
PANTHER	PTHR10584:SF24. Adenokinase. 1 hit.
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
PRINTS	PR00989. ADENOKINASE.
PROSITE	PS00583. PFKB_KINASES_1. False negative. PS00584. PFKB_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADK_CRIGR

Accession

Primary (citable) accession number: P55262

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 30, 2000
Last modified:	September 22, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)