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P55258

- RAB8A_MOUSE

UniProt

P55258 - RAB8A_MOUSE

Protein

Ras-related protein Rab-8A

Gene

Rab8a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 2 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis.1 Publication

    Enzyme regulationi

    Activated in response to insulin.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 228GTPBy similarity
    Nucleotide bindingi63 – 675GTPBy similarity
    Nucleotide bindingi121 – 1244GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: UniProtKB
    2. GTPase activity Source: Ensembl
    3. GTP binding Source: UniProtKB
    4. protein binding Source: UniProtKB
    5. Rab GTPase binding Source: UniProtKB

    GO - Biological processi

    1. axonogenesis Source: UniProtKB
    2. cellular response to insulin stimulus Source: UniProtKB
    3. cilium assembly Source: UniProtKB
    4. Golgi vesicle fusion to target membrane Source: Ensembl
    5. protein localization to plasma membrane Source: UniProtKB
    6. protein transport Source: UniProtKB-KW
    7. regulation of long-term neuronal synaptic plasticity Source: Ensembl
    8. regulation of protein transport Source: Ensembl
    9. small GTPase mediated signal transduction Source: InterPro
    10. vesicle docking involved in exocytosis Source: Ensembl

    Keywords - Biological processi

    Cilium biogenesis/degradation, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196635. Regulation of PLK1 Activity at G2/M Transition.
    REACT_199054. Translocation of GLUT4 to the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-8A
    Alternative name(s):
    Oncogene c-mel
    Gene namesi
    Name:Rab8a
    Synonyms:Mel, Rab8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:96960. Rab8a.

    Subcellular locationi

    Cell membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication. Golgi apparatus By similarity. Recycling endosome membrane 1 Publication. Cell projectioncilium By similarity. Cytoplasmic vesiclephagosome By similarity. Cytoplasmic vesiclephagosome membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
    Note: Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis. Localizes to tubular recycling endosome. Recruited to phagosomes containing S.aureus or Mycobacterium By similarity.By similarity

    GO - Cellular componenti

    1. centrosome Source: MGI
    2. ciliary basal body Source: MGI
    3. cilium Source: MGI
    4. dendrite Source: Ensembl
    5. neuronal cell body Source: Ensembl
    6. nonmotile primary cilium Source: MGI
    7. phagocytic vesicle Source: UniProtKB
    8. phagocytic vesicle membrane Source: UniProtKB-SubCell
    9. plasma membrane Source: UniProtKB
    10. postsynaptic density Source: Ensembl
    11. recycling endosome Source: UniProtKB
    12. recycling endosome membrane Source: UniProtKB
    13. trans-Golgi network transport vesicle Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 204204Ras-related protein Rab-8APRO_0000121132Add
    BLAST
    Propeptidei205 – 2073Removed in mature formSequence AnalysisPRO_0000370795

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei181 – 1811PhosphoserineBy similarity
    Modified residuei185 – 1851PhosphoserineBy similarity
    Modified residuei204 – 2041Cysteine methyl esterSequence Analysis
    Lipidationi204 – 2041S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Proteomic databases

    MaxQBiP55258.
    PaxDbiP55258.
    PRIDEiP55258.

    PTM databases

    PhosphoSiteiP55258.

    Expressioni

    Gene expression databases

    ArrayExpressiP55258.
    BgeeiP55258.
    CleanExiMM_RAB8A.
    GenevestigatoriP55258.

    Interactioni

    Subunit structurei

    Interacts (GTP-bound form) with MICALL1; regulates RAB8A association with recycling endosomes. Interacts with MICALL2; competes with RAB13 and is involved in E-cadherin endocytic recycling. Interacts with MICAL1. Interacts with EHD1. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with RABIF, RIMS2, RPH3A and RPH3A. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL.7 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Rph3aP477082EBI-398411,EBI-398376

    Protein-protein interaction databases

    BioGridi201387. 5 interactions.
    IntActiP55258. 11 interactions.
    MINTiMINT-1862635.

    Structurei

    Secondary structure

    1
    207
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 149
    Helixi37 – 415
    Beta strandi43 – 5210
    Beta strandi55 – 639
    Turni76 – 805
    Beta strandi82 – 898
    Helixi93 – 10917
    Beta strandi115 – 1217
    Helixi133 – 14210
    Beta strandi146 – 1494
    Helixi158 – 17518

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FU5X-ray2.00C/D1-175[»]
    ProteinModelPortaliP55258.
    SMRiP55258. Positions 6-176.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP55258.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi37 – 459Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    KOiK07901.
    OMAiIDKAFYS.
    OrthoDBiEOG7VB2H4.
    TreeFamiTF314097.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P55258-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI    50
    ELDGKRIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR 100
    NWIRNIEEHA SADVEKMILG NKCDVNDKRQ VSKERGEKLA LDYGIKFMET 150
    SAKANINVEN AFFTLARDIK AKMDKKLEGN SPQGSSHGVK ITVEQQKRTS 200
    FFRCSLL 207
    Length:207
    Mass (Da):23,668
    Last modified:November 22, 2005 - v2
    Checksum:iAC89DC85588FB8F8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti176 – 1838KLEGNSPQ → NWKATAA in AAB19682. (PubMed:1886711)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S53270 mRNA. Translation: AAB19682.1.
    AK076048 mRNA. Translation: BAC36146.1.
    AK079306 mRNA. Translation: BAC37603.1.
    AK080740 mRNA. Translation: BAC38003.1.
    BC019990 mRNA. Translation: AAH19990.1.
    CCDSiCCDS22409.1.
    RefSeqiNP_075615.2. NM_023126.2.
    UniGeneiMm.162811.

    Genome annotation databases

    EnsembliENSMUST00000003121; ENSMUSP00000003121; ENSMUSG00000003037.
    GeneIDi17274.
    KEGGimmu:17274.
    UCSCiuc009mfj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S53270 mRNA. Translation: AAB19682.1 .
    AK076048 mRNA. Translation: BAC36146.1 .
    AK079306 mRNA. Translation: BAC37603.1 .
    AK080740 mRNA. Translation: BAC38003.1 .
    BC019990 mRNA. Translation: AAH19990.1 .
    CCDSi CCDS22409.1.
    RefSeqi NP_075615.2. NM_023126.2.
    UniGenei Mm.162811.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FU5 X-ray 2.00 C/D 1-175 [» ]
    ProteinModelPortali P55258.
    SMRi P55258. Positions 6-176.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201387. 5 interactions.
    IntActi P55258. 11 interactions.
    MINTi MINT-1862635.

    PTM databases

    PhosphoSitei P55258.

    Proteomic databases

    MaxQBi P55258.
    PaxDbi P55258.
    PRIDEi P55258.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000003121 ; ENSMUSP00000003121 ; ENSMUSG00000003037 .
    GeneIDi 17274.
    KEGGi mmu:17274.
    UCSCi uc009mfj.1. mouse.

    Organism-specific databases

    CTDi 4218.
    MGIi MGI:96960. Rab8a.

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    KOi K07901.
    OMAi IDKAFYS.
    OrthoDBi EOG7VB2H4.
    TreeFami TF314097.

    Enzyme and pathway databases

    Reactomei REACT_196635. Regulation of PLK1 Activity at G2/M Transition.
    REACT_199054. Translocation of GLUT4 to the plasma membrane.

    Miscellaneous databases

    EvolutionaryTracei P55258.
    NextBioi 291770.
    PROi P55258.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P55258.
    Bgeei P55258.
    CleanExi MM_RAB8A.
    Genevestigatori P55258.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The MEL gene: a new member of the RAB/YPT class of RAS-related genes."
      Nimmo E.R., Sanders P.G., Padua R.A., Hughes D., Williamson R., Johnson K.J.
      Oncogene 6:1347-1351(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum and Retina.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    4. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 11-21; 59-69 AND 139-153, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    5. "In its active form, the GTP-binding protein rab8 interacts with a stress-activated protein kinase."
      Ren M., Zeng J., De Lemos-Chiarandini C., Rosenfeld M., Adesnik M., Sabatini D.D.
      Proc. Natl. Acad. Sci. U.S.A. 93:5151-5155(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAP4K2.
      Strain: BALB/c.
      Tissue: Melanoma.
    6. "Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2."
      Fukuda M.
      J. Biol. Chem. 278:15373-15380(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RIMS2; RPH3A AND RPH3AL.
    7. "Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells."
      Fukuda M.
      J. Biol. Chem. 278:15390-15396(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SYTL4.
    8. "Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway."
      Yang H., Sasaki T., Minoshima S., Shimizu N.
      Genomics 90:249-260(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SGSM1 AND SGSM3.
    9. "The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions."
      Yamamura R., Nishimura N., Nakatsuji H., Arase S., Sasaki T.
      Mol. Biol. Cell 19:971-983(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN ENDOCYTIC RECYCLING, SUBCELLULAR LOCATION, INTERACTION WITH MICAL1; MICALL1 AND MICALL2.
    10. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: INTERACTION WITH PIFO.
    12. "Nucleotide exchange via local protein unfolding--structure of Rab8 in complex with MSS4."
      Itzen A., Pylypenko O., Goody R.S., Alexandrov K., Rak A.
      EMBO J. 25:1445-1455(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-175 IN COMPLEX WITH RABIF, INTERACTION WITH RABIF.

    Entry informationi

    Entry nameiRAB8A_MOUSE
    AccessioniPrimary (citable) accession number: P55258
    Secondary accession number(s): Q8VCF6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 137 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3