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Protein

Ras-related protein Rab-8A

Gene

Rab8a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis.1 Publication

Enzyme regulationi

Activated in response to insulin.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTPBy similarity8
Nucleotide bindingi63 – 67GTPBy similarity5
Nucleotide bindingi121 – 124GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-5620916. VxPx cargo-targeting to cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-8A
Alternative name(s):
Oncogene c-mel
Gene namesi
Name:Rab8a
Synonyms:Mel, Rab8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:96960. Rab8a.

Subcellular locationi

GO - Cellular componenti

  • centriole Source: UniProtKB-SubCell
  • centrosome Source: MGI
  • ciliary basal body Source: MGI
  • ciliary base Source: MGI
  • cilium Source: MGI
  • dendrite Source: Ensembl
  • extracellular exosome Source: MGI
  • extracellular matrix Source: MGI
  • neuronal cell body Source: Ensembl
  • non-motile cilium Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: MGI
  • phagocytic vesicle Source: UniProtKB
  • phagocytic vesicle membrane Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
  • postsynaptic density Source: Ensembl
  • recycling endosome Source: UniProtKB
  • recycling endosome membrane Source: UniProtKB
  • synaptic vesicle Source: GO_Central
  • trans-Golgi network transport vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001211321 – 204Ras-related protein Rab-8AAdd BLAST204
PropeptideiPRO_0000370795205 – 207Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181PhosphoserineBy similarity1
Modified residuei185PhosphoserineCombined sources1
Modified residuei204Cysteine methyl esterSequence analysis1
Lipidationi204S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiP55258.
PaxDbiP55258.
PeptideAtlasiP55258.
PRIDEiP55258.

PTM databases

iPTMnetiP55258.
PhosphoSitePlusiP55258.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003037.
CleanExiMM_RAB8A.
ExpressionAtlasiP55258. baseline and differential.
GenevisibleiP55258. MM.

Interactioni

Subunit structurei

Interacts (GTP-bound form) with MICALL1; regulates RAB8A association with recycling endosomes. Interacts with MICALL2; competes with RAB13 and is involved in E-cadherin endocytic recycling. Interacts with MICAL1. Interacts with EHD1. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with RABIF, RIMS2, RPH3A and RPH3A. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL. Interacts with AHI1 (PubMed:19625297).8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rph3aP477082EBI-398411,EBI-398376
SncaO550422EBI-398411,EBI-2310271

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201387. 8 interactors.
DIPiDIP-31053N.
IntActiP55258. 22 interactors.
MINTiMINT-1862635.
STRINGi10090.ENSMUSP00000003121.

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 14Combined sources9
Helixi37 – 41Combined sources5
Beta strandi43 – 52Combined sources10
Beta strandi55 – 63Combined sources9
Turni76 – 80Combined sources5
Beta strandi82 – 89Combined sources8
Helixi93 – 109Combined sources17
Beta strandi115 – 121Combined sources7
Helixi133 – 142Combined sources10
Beta strandi146 – 149Combined sources4
Helixi158 – 175Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FU5X-ray2.00C/D1-175[»]
ProteinModelPortaliP55258.
SMRiP55258.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55258.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0078. Eukaryota.
ENOG410XPUI. LUCA.
GeneTreeiENSGT00860000133675.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP55258.
KOiK07901.
OMAiFTNIRTW.
OrthoDBiEOG091G0LA6.
TreeFamiTF314097.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55258-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI
60 70 80 90 100
ELDGKRIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR
110 120 130 140 150
NWIRNIEEHA SADVEKMILG NKCDVNDKRQ VSKERGEKLA LDYGIKFMET
160 170 180 190 200
SAKANINVEN AFFTLARDIK AKMDKKLEGN SPQGSSHGVK ITVEQQKRTS

FFRCSLL
Length:207
Mass (Da):23,668
Last modified:November 22, 2005 - v2
Checksum:iAC89DC85588FB8F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176 – 183KLEGNSPQ → NWKATAA in AAB19682 (PubMed:1886711).Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53270 mRNA. Translation: AAB19682.1.
AK076048 mRNA. Translation: BAC36146.1.
AK079306 mRNA. Translation: BAC37603.1.
AK080740 mRNA. Translation: BAC38003.1.
BC019990 mRNA. Translation: AAH19990.1.
CCDSiCCDS22409.1.
RefSeqiNP_075615.2. NM_023126.2.
UniGeneiMm.162811.

Genome annotation databases

EnsembliENSMUST00000003121; ENSMUSP00000003121; ENSMUSG00000003037.
GeneIDi17274.
KEGGimmu:17274.
UCSCiuc009mfj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53270 mRNA. Translation: AAB19682.1.
AK076048 mRNA. Translation: BAC36146.1.
AK079306 mRNA. Translation: BAC37603.1.
AK080740 mRNA. Translation: BAC38003.1.
BC019990 mRNA. Translation: AAH19990.1.
CCDSiCCDS22409.1.
RefSeqiNP_075615.2. NM_023126.2.
UniGeneiMm.162811.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FU5X-ray2.00C/D1-175[»]
ProteinModelPortaliP55258.
SMRiP55258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201387. 8 interactors.
DIPiDIP-31053N.
IntActiP55258. 22 interactors.
MINTiMINT-1862635.
STRINGi10090.ENSMUSP00000003121.

PTM databases

iPTMnetiP55258.
PhosphoSitePlusiP55258.

Proteomic databases

EPDiP55258.
PaxDbiP55258.
PeptideAtlasiP55258.
PRIDEiP55258.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003121; ENSMUSP00000003121; ENSMUSG00000003037.
GeneIDi17274.
KEGGimmu:17274.
UCSCiuc009mfj.1. mouse.

Organism-specific databases

CTDi4218.
MGIiMGI:96960. Rab8a.

Phylogenomic databases

eggNOGiKOG0078. Eukaryota.
ENOG410XPUI. LUCA.
GeneTreeiENSGT00860000133675.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP55258.
KOiK07901.
OMAiFTNIRTW.
OrthoDBiEOG091G0LA6.
TreeFamiTF314097.

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-5620916. VxPx cargo-targeting to cilium.

Miscellaneous databases

EvolutionaryTraceiP55258.
PROiP55258.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003037.
CleanExiMM_RAB8A.
ExpressionAtlasiP55258. baseline and differential.
GenevisibleiP55258. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB8A_MOUSE
AccessioniPrimary (citable) accession number: P55258
Secondary accession number(s): Q8VCF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 22, 2005
Last modified: November 30, 2016
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.