P55258 (RAB8A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-8A

Alternative name(s):
Oncogene c-mel

Gene names

Name:	Rab8a
Synonyms:	Mel, Rab8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization By similarity.
Subunit structure	Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3 By similarity. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B By similarity. Interacts with PIFO. Interacts with BIRC6/bruce By similarity. Interacts with OCRL By similarity. Interacts with RABIF, RIMS2, RPH3A and RPH3A. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Golgi apparatus By similarity. Cytoplasm › perinuclear region By similarity. Cell projection By similarity. Cytoplasmic vesicle › phagosome By similarity. Cytoplasmic vesicle › phagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis. Recruited to phagosomes containing S.aureus or Mycobacterium By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Cell projection Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane
Disease	Proto-oncogene
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Golgi vesicle fusion to target membrane Inferred from electronic annotation. Source: Compara axonogenesis Inferred from sequence or structural similarity. Source: UniProtKB cilium assembly Inferred from electronic annotation. Source: Compara protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of long-term neuronal synaptic plasticity Inferred from electronic annotation. Source: Compara regulation of protein transport Inferred from electronic annotation. Source: Compara small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro vesicle docking involved in exocytosis Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule basal body Inferred from direct assay PubMed 19625297. Source: MGI neuronal cell body Inferred from electronic annotation. Source: Compara nonmotile primary cilium Inferred from direct assay PubMed 19625297. Source: MGI perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell phagocytic vesicle Inferred from sequence or structural similarity. Source: UniProtKB phagocytic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic density Inferred from electronic annotation. Source: Compara recycling endosome Inferred from direct assay PubMed 19061864. Source: MGI
Molecular_function	GDP binding Inferred from sequence or structural similarity. Source: UniProtKB GTP binding Inferred from sequence or structural similarity. Source: UniProtKB Rab GTPase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Rph3a	P47708	2	EBI-398411,EBI-398376

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 204

204

Ras-related protein Rab-8A

PRO_0000121132

Propeptide

205 – 207

3

Removed in mature form Potential

PRO_0000370795

Regions

Nucleotide binding

15 – 22

8

GTP By similarity

Nucleotide binding

63 – 67

5

GTP By similarity

Nucleotide binding

121 – 124

4

GTP By similarity

Motif

37 – 45

9

Effector region By similarity

Amino acid modifications

Modified residue

181

1

Phosphoserine By similarity

Modified residue

185

1

Phosphoserine By similarity

Modified residue

204

1

Cysteine methyl ester Potential

Lipidation

204

1

S-geranylgeranyl cysteine By similarity

Experimental info

Sequence conflict

176 – 183

8

KLEGNSPQ → NWKATAA in AAB19682. Ref.1

Secondary structure

1

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207

Helix Strand Turn

Details...

Beta strand

6 – 14

9

Helix

37 – 41

5

Beta strand

43 – 52

10

Beta strand

55 – 63

9

Turn

76 – 80

5

Beta strand

82 – 89

8

Helix

93 – 109

17

Beta strand

115 – 121

7

Helix

133 – 142

10

Beta strand

146 – 149

4

Helix

158 – 175

18

Sequences

	Sequence		Length	Mass (Da)
	P55258 [UniParc]. Last modified November 22, 2005. Version 2. Checksum: AC89DC85588FB8F8	FASTA	207	23,668
	10 20 30 40 50 60 MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI ELDGKRIKLQ 70 80 90 100 110 120 IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR NWIRNIEEHA SADVEKMILG 130 140 150 160 170 180 NKCDVNDKRQ VSKERGEKLA LDYGIKFMET SAKANINVEN AFFTLARDIK AKMDKKLEGN 190 200 SPQGSSHGVK ITVEQQKRTS FFRCSLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The MEL gene: a new member of the RAB/YPT class of RAS-related genes." Nimmo E.R., Sanders P.G., Padua R.A., Hughes D., Williamson R., Johnson K.J. Oncogene 6:1347-1351(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Cerebellum and Retina.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
[4]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 11-21; 59-69 AND 139-153, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[5]	"In its active form, the GTP-binding protein rab8 interacts with a stress-activated protein kinase." Ren M., Zeng J., De Lemos-Chiarandini C., Rosenfeld M., Adesnik M., Sabatini D.D. Proc. Natl. Acad. Sci. U.S.A. 93:5151-5155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAP4K2. Strain: BALB/c. Tissue: Melanoma.
[6]	"Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2." Fukuda M. J. Biol. Chem. 278:15373-15380(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RIMS2; RPH3A AND RPH3AL.
[7]	"Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells." Fukuda M. J. Biol. Chem. 278:15390-15396(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SYTL4.
[8]	"Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway." Yang H., Sasaki T., Minoshima S., Shimizu N. Genomics 90:249-260(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SGSM1 AND SGSM3.
[9]	"Pitchfork regulates primary cilia disassembly and left-right asymmetry." Kinzel D., Boldt K., Davis E.E., Burtscher I., Trumbach D., Diplas B., Attie-Bitach T., Wurst W., Katsanis N., Ueffing M., Lickert H. Dev. Cell 19:66-77(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PIFO.
[10]	"Nucleotide exchange via local protein unfolding--structure of Rab8 in complex with MSS4." Itzen A., Pylypenko O., Goody R.S., Alexandrov K., Rak A. EMBO J. 25:1445-1455(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-175 IN COMPLEX WITH RABIF, INTERACTION WITH RABIF.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

S53270 mRNA. Translation: AAB19682.1.
AK076048 mRNA. Translation: BAC36146.1.
AK079306 mRNA. Translation: BAC37603.1.
AK080740 mRNA. Translation: BAC38003.1.
BC019990 mRNA. Translation: AAH19990.1.

IPI

IPI00331128.

RefSeq

NP_075615.2. NM_023126.2.

UniGene

Mm.162811.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FU5	X-ray	2.00	C/D	1-175	[»]

ProteinModelPortal

P55258.

SMR

P55258. Positions 6-176.

ModBase

Search...

Protein-protein interaction databases

IntAct

P55258. 9 interactions.

PTM databases

PhosphoSite

P55258.

Proteomic databases

PaxDb

P55258.

PRIDE

P55258.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000003121; ENSMUSP00000003121; ENSMUSG00000003037.

GeneID

17274.

KEGG

mmu:17274.

Organism-specific databases

CTD

4218.

MGI

MGI:96960. Rab8a.

Phylogenomic databases

eggNOG

COG1100.

HOGENOM

HOG000233968.

HOVERGEN

HBG009351.

KO

K07901.

OMA

SNVEQHA.

OrthoDB

EOG4K3KX6.

Enzyme and pathway databases

Reactome

REACT_147847. Translocation of Glut4 to the Plasma Membrane.

Gene expression databases

ArrayExpress

P55258.

Bgee

P55258.

CleanEx

MM_RAB8A.

Genevestigator

P55258.

GermOnline

ENSMUSG00000003037. Mus musculus.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P55258.

NextBio

291770.

SOURCE

Search...

Entry information

Entry name

RAB8A_MOUSE

Accession

Primary (citable) accession number: P55258
Secondary accession number(s): Q8VCF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program