P55255 (RMLA_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-1-phosphate thymidylyltransferase
EC=2.7.7.24

Alternative name(s):
dTDP-glucose pyrophosphorylase
dTDP-glucose synthase

Gene names

Name:	rmlA1
Synonyms:	rfbA1
Ordered Locus Names:	NMB0062
AND
Name:	rmlA2
Synonyms:	rfbA2
Ordered Locus Names:	NMB0080

Organism

Neisseria meningitidis serogroup B

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis By similarity.
Catalytic activity	dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glucose-1-phosphate thymidylyltransferase family.
Sequence caution	The sequence AAC37050.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	extracellular polysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	glucose-1-phosphate thymidylyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Glucose-1-phosphate thymidylyltransferase		PRO_0000207998
Sites
	Metal binding	108	1	Magnesium By similarity
	Metal binding	223	1	Magnesium By similarity
Experimental info
	Sequence conflict	125	1	A → R in AAC37050. Ref.1
	Sequence conflict	198	1	T → S in AAC37050. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P55255 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 8A65B50B531F2907	FASTA	288	32,162
	10 20 30 40 50 60 MKGIILAGGS GTRLYPITRG VSKQLLPVYD KPMIYYPLSV LMLAGIRDIL VITAPEDNAS 70 80 90 100 110 120 FKRLLGDGSD FGISISYAVQ PSPDGLAQAF IIGEEFIGND NVCLVLGDNI FYGQSFTQTL 130 140 150 160 170 180 KQAAAQTHGA TVFAYQVKNP ERFGVVEFNE NFRAVSIEEK PQRPKSDWAV TGLYFYDNRA 190 200 210 220 230 240 VEFAKQLKPS ARGELEITDL NRMYLEDGSL SVQILGRGFA WLDTGTHESL HEAASFVQTV 250 260 270 280 QNIQNLHIAC LEEIAWRNGW LSDEKLEELA RPMAKNQYGQ YLLRLLKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Contribution of genes from the capsule gene complex (cps) to lipooligosaccharide biosynthesis and serum resistance in Neisseria meningitidis." Hammerschmidt S., Birkholz C., Zaehringer U., Robertson B.D., van Putten J.P.M., Ebeling O., Frosch M. Mol. Microbiol. 11:885-896(1994) [PubMed: 8022265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B1940 / Serogroup B.
[2]	"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L09189 Genomic DNA. Translation: AAC37050.1. Different initiation. AE002098 Genomic DNA. Translation: AAF40530.1. AE002098 Genomic DNA. Translation: AAF40544.1.
PIR	B81240.
RefSeq	NP_273126.1. NC_003112.2. NP_273143.1. NC_003112.2.
3D structure databases
ProteinModelPortal	P55255.
SMR	P55255. Positions 1-287.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000010814; EBNEIP00000010434; EBNEIG00000010814. EBNEIT00000010900; EBNEIP00000010520; EBNEIG00000010900.
GeneID	902169. 902184.
GenomeReviews	Gene locus NMB0062 in contig AE002098_GR. Gene locus NMB0080 in contig AE002098_GR.
KEGG	nme:NMB0062. nme:NMB0080.
PATRIC	20355129. VBINeiMen85645_0098.
TIGR	NMB0062. NMB0080.
Phylogenomic databases
GeneTree	EBGT00050000021321.
HOGENOM	HBG688195.
OMA	EDNASFK.
ProtClustDB	CLSK2517808.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0062-MONOMER. NMEN122586:NMB_0080-MONOMER.
Family and domain databases
InterPro	IPR005907. G1P_thy_trans_s. IPR005835. NTP_transferase. [Graphical view]
KO	K00973.
PANTHER	PTHR22572:SF13. PTHR22572:SF13. 1 hit.
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01207. RmlA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RMLA_NEIMB

Accession

Primary (citable) accession number: P55255

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program