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P55249 (LX12E_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
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Names and origin

Protein namesRecommended name:
Arachidonate 12-lipoxygenase, epidermal-type
Short name=12-LOX
EC=1.13.11.31
Gene names
Name:Alox12e
Synonyms:Alox12-ps2, Aloxe
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length662 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Arachidonate + O2 = (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate.

Cofactor

Binds 1 iron ion per subunit.

Pathway

Lipid metabolism; leukotriene D4 biosynthesis.

Subcellular location

Cytoplasm Potential.

Tissue specificity

Epidermal.

Sequence similarities

Belongs to the lipoxygenase family.

Contains 1 lipoxygenase domain.

Contains 1 PLAT domain.

Caution

Was originally thought to be an arachidonate 8-lipoxygenase and was called LOX8.

Ontologies

Keywords
   Biological processLeukotriene biosynthesis
   Cellular componentCytoplasm
   LigandIron
Metal-binding
   Molecular functionDioxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleukotriene biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionarachidonate 12-lipoxygenase activity

Inferred from electronic annotation. Source: EC

iron ion binding

Inferred from electronic annotation. Source: InterPro

lipoxygenase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 662662Arachidonate 12-lipoxygenase, epidermal-type
PRO_0000220688

Regions

Domain2 – 114113PLAT
Domain115 – 662548Lipoxygenase

Sites

Metal binding3601Iron; catalytic By similarity
Metal binding3651Iron; catalytic By similarity
Metal binding5401Iron; catalytic By similarity
Metal binding6621Iron; via carboxylate; catalytic By similarity

Natural variations

Natural variant2601V → L in strain:FVB/N. Ref.4
Natural variant4531V → L in strain:FVB/N. Ref.4
Natural variant6171P → S in strain:FVB/N. Ref.4

Experimental info

Sequence conflict6191P → A in AAC52324. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P55249 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: D67768415EE988F7

FASTA66275,456
        10         20         30         40         50         60 
MVKYKILVAT GDSVFAGSAN LVHLWLVGEH GEADLGKQLR PLLGRKTELE VDVPLHLGRL 

        70         80         90        100        110        120 
LAVKLRKQKG LLDSDWFCKS ITVQGPGTQG EAFFPCYSWV QGKETICLTE GTALKVTDDT 

       130        140        150        160        170        180 
QNLFRKYREQ ELENRRNVYR WGSWKEGLIL PIAGSTERDL PRNQRFMKDK DLDFSLSLVK 

       190        200        210        220        230        240 
ELKNFAIKGT LDFVSRVQKL EDYQKVFPHT KTALPERVRG SWKEDALFGY QFLNGANPML 

       250        260        270        280        290        300 
LRRSMRLPAR LVLPPGMEDV QTQLEKELKA GSLFEVDFSL LDGVKPNIII FKQQYVTAPL 

       310        320        330        340        350        360 
VMLKLQPDGR LLPMVIQLQP PRHGCPPPLL FLPSDPPMAW LLAKIWVRSS DFQLHQLQSH 

       370        380        390        400        410        420 
LLRGHLMAEV ISVATMRSLP SLHPIYKLLA PHFRYTMEIN TLARNNLVSE WGIFDLVVST 

       430        440        450        460        470        480 
GSGGHVDILQ RATSCLTYRS FCPPDDLADR GLVGVKSSLY AQDALRLWEI ISRYVERMVE 

       490        500        510        520        530        540 
LFYRSDTDVK EDPELQVWCR EVTEVGLLGA QDRGFPLSLE SRAELCRFVA MCIFTCTGQH 

       550        560        570        580        590        600 
ASTHLGQLDW YAWIPNGPCT MRKPPPISKD VTERDIVDSL PCLQQARMQI TVTKFLGRRQ 

       610        620        630        640        650        660 
PVMVALGQHK EEYFSGPRPR DVLKQFQEEL AIMDKEIEVR NASLDLPYEY LRPSLVENSV 


TI

« Hide

References

« Hide 'large scale' references
[1]"Functional expression and cellular localization of a mouse epidermal lipoxygenase."
Funk C.D., Keeney D.S., Oliw E.H., Boeglin W.E., Brash A.R.
J. Biol. Chem. 271:23338-23344(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X 129/Sv.
Tissue: Epidermis.
[2]"Genomic and cDNA cloning of a novel mouse lipoxygenase gene."
van Dijk K.W., Steketee K., Havekes L., Frants R., Hofker M.
Biochim. Biophys. Acta 1259:4-8(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
Tissue: Epidermis.
[3]"Murine epidermal lipoxygenase (Aloxe) encodes a 12-lipoxygenase isoform."
Kinzig A., Fuerstenberger G., Mueller F., Vogel S., Mueller-Decker K., Mincheva A., Lichter P., Marks F., Krieg P.
FEBS Lett. 402:162-166(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: NMRI.
Tissue: Skin.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS LEU-260; LEU-453 AND SER-617.
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U39200 mRNA. Translation: AAC52869.1.
U24181 Genomic DNA. Translation: AAC52324.1.
X99252 mRNA. Translation: CAA67625.1.
BC013751 mRNA. Translation: AAH13751.1.
BC051047 mRNA. Translation: AAH51047.1.
IPIIPI00137960.
RefSeqNP_663717.1. NM_145684.1.
UniGeneMm.274093.

3D structure databases

ProteinModelPortalP55249.
SMRP55249. Positions 4-662.
ModBaseSearch...

PTM databases

PhosphoSiteP55249.

Proteomic databases

PRIDEP55249.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019051; ENSMUSP00000019051; ENSMUSG00000018907.
GeneID11685.
KEGGmmu:11685.

Organism-specific databases

CTD11685.
MGIMGI:1274790. Alox12e.

Phylogenomic databases

eggNOGNOG133298.
HOGENOMHOG000234358.
HOVERGENHBG005150.
InParanoidP55249.
KOK00458.
OMAKFLGRRQ.
OrthoDBEOG4W0XCM.

Enzyme and pathway databases

UniPathwayUPA00880.

Gene expression databases

ArrayExpressP55249.
BgeeP55249.
CleanExMM_ALOX12E.
GenevestigatorP55249.
GermOnlineENSMUSG00000018907. Mus musculus.

Family and domain databases

Gene3D2.60.60.20. 1 hit.
InterProIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001024. LipOase_LH2.
IPR001885. LipOase_mml.
[Graphical view]
PANTHERPTHR11771. PTHR11771. 1 hit.
PfamPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSPR00087. LIPOXYGENASE.
PR00467. MAMLPOXGNASE.
SMARTSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMSSF49723. Lipase_LipOase. 1 hit.
SSF48484. Lipoxygenase. 1 hit.
PROSITEPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279323.
SOURCESearch...

Entry information

Entry nameLX12E_MOUSE
AccessionPrimary (citable) accession number: P55249
Secondary accession number(s): Q91YW6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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