Reviewed, UniProtKB/Swiss-Prot P55249 (LX12E_MOUSE)

Names and origin

Protein names

Recommended name:
    Arachidonate 12-lipoxygenase, epidermal-type
      Short name=12-LOX
    EC=1.13.11.31

Gene names

Name:	Alox12e
Synonyms:	Alox12-ps2, Aloxe

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	662 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Arachidonate + O2 = (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate.
Cofactor	Binds 1 iron ion per subunit.
Pathway	Lipid metabolism; leukotriene D4 biosynthesis.
Subcellular location	Cytoplasm Potential.
Tissue specificity	Epidermal.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.
Caution	Was originally thought to be an arachidonate 8-lipoxygenase and was called LOX8.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arachidonate 12-lipoxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 662	662	Arachidonate 12-lipoxygenase, epidermal-type		PRO_0000220688
Regions
	Domain	2 – 114	113	PLAT
	Domain	115 – 662	548	Lipoxygenase
Sites
	Metal binding	360	1	Iron; catalytic By similarity
	Metal binding	365	1	Iron; catalytic By similarity
	Metal binding	540	1	Iron; catalytic By similarity
	Metal binding	662	1	Iron; via carboxylate; catalytic By similarity
Natural variations
	Natural variant	260	1	V → L in strain:FVB/N. Ref.4
	Natural variant	453	1	V → L in strain:FVB/N. Ref.4
	Natural variant	617	1	P → S in strain:FVB/N. Ref.4
Experimental info
	Sequence conflict	619	1	P → A in AAC52324. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P55249-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D67768415EE988F7	FASTA	662	75,456
	10 20 30 40 50 60 MVKYKILVAT GDSVFAGSAN LVHLWLVGEH GEADLGKQLR PLLGRKTELE VDVPLHLGRL 70 80 90 100 110 120 LAVKLRKQKG LLDSDWFCKS ITVQGPGTQG EAFFPCYSWV QGKETICLTE GTALKVTDDT 130 140 150 160 170 180 QNLFRKYREQ ELENRRNVYR WGSWKEGLIL PIAGSTERDL PRNQRFMKDK DLDFSLSLVK 190 200 210 220 230 240 ELKNFAIKGT LDFVSRVQKL EDYQKVFPHT KTALPERVRG SWKEDALFGY QFLNGANPML 250 260 270 280 290 300 LRRSMRLPAR LVLPPGMEDV QTQLEKELKA GSLFEVDFSL LDGVKPNIII FKQQYVTAPL 310 320 330 340 350 360 VMLKLQPDGR LLPMVIQLQP PRHGCPPPLL FLPSDPPMAW LLAKIWVRSS DFQLHQLQSH 370 380 390 400 410 420 LLRGHLMAEV ISVATMRSLP SLHPIYKLLA PHFRYTMEIN TLARNNLVSE WGIFDLVVST 430 440 450 460 470 480 GSGGHVDILQ RATSCLTYRS FCPPDDLADR GLVGVKSSLY AQDALRLWEI ISRYVERMVE 490 500 510 520 530 540 LFYRSDTDVK EDPELQVWCR EVTEVGLLGA QDRGFPLSLE SRAELCRFVA MCIFTCTGQH 550 560 570 580 590 600 ASTHLGQLDW YAWIPNGPCT MRKPPPISKD VTERDIVDSL PCLQQARMQI TVTKFLGRRQ 610 620 630 640 650 660 PVMVALGQHK EEYFSGPRPR DVLKQFQEEL AIMDKEIEVR NASLDLPYEY LRPSLVENSV TI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional expression and cellular localization of a mouse epidermal lipoxygenase." Funk C.D., Keeney D.S., Oliw E.H., Boeglin W.E., Brash A.R. J. Biol. Chem. 271:23338-23344(1996) [PubMed: 8798535] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X 129/Sv. Tissue: Epidermis.
[2]	"Genomic and cDNA cloning of a novel mouse lipoxygenase gene." van Dijk K.W., Steketee K., Havekes L., Frants R., Hofker M. Biochim. Biophys. Acta 1259:4-8(1995) [PubMed: 7492614] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv. Tissue: Epidermis.
[3]	"Murine epidermal lipoxygenase (Aloxe) encodes a 12-lipoxygenase isoform." Kinzig A., Fuerstenberger G., Mueller F., Vogel S., Mueller-Decker K., Mincheva A., Lichter P., Marks F., Krieg P. FEBS Lett. 402:162-166(1997) [PubMed: 9037187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: NMRI. Tissue: Skin.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS LEU-260; LEU-453 AND SER-617. Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U39200 mRNA. Translation: AAC52869.1. U24181 Genomic DNA. Translation: AAC52324.1. X99252 mRNA. Translation: CAA67625.1. BC013751 mRNA. Translation: AAH13751.1. BC051047 mRNA. Translation: AAH51047.1.
IPI	IPI00137960.
RefSeq	NP_663717.1.
UniGene	Mm.274093
3D structure databases
HSSP	HSSP built from PDB template 1LOX based on UniProtKB P12530.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000018907. Mus musculus. [Contig view]
GeneID	11685.
KEGG	mmu:11685.
Organism-specific databases
MGI	MGI:1274790. Alox12e.
Phylogenomic databases
HOGENOM	P55249.
HOVERGEN	P55249.
OMA	P55249. SGGHVDI.
Enzyme and pathway databases
BRENDA	1.13.11.31. 244.
Gene expression databases
ArrayExpress	P55249.
Bgee	P55249.
CleanEx	MM_ALOX12E.
GermOnline	ENSMUSG00000018907. Mus musculus.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001885. LipOase_mml. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00467. MAMLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	279323.
SOURCE	Search...

Entry information

Entry name

LX12E_MOUSE

Accession

Primary (citable) accession number: P55249
Secondary accession number(s): Q91YW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)