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Protein

Glucose-1-phosphate adenylyltransferase large subunit 3, chloroplastic

Gene

APL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This protein plays a role in synthesis of starch. It catalyzes the synthesis of the activated glycosyl donor, ADP-glucose from Glc-1-P and ATP.

Catalytic activityi

ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-glucose.

Enzyme regulationi

Activated by 3'phosphoglycerate, inhibited by orthophosphate. Allosteric regulation.

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glucose-1-phosphate adenylyltransferase activity Source: TAIR

GO - Biological processi

  • glycogen biosynthetic process Source: InterPro
  • starch biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Starch biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.7.27. 399.
SABIO-RKP55231.
UniPathwayiUPA00152.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-1-phosphate adenylyltransferase large subunit 3, chloroplastic (EC:2.7.7.27)
Alternative name(s):
ADP-glucose pyrophosphorylase
ADP-glucose synthase
AGPase S
Alpha-D-glucose-1-phosphate adenyl transferase
Gene namesi
Name:APL3
Ordered Locus Names:At4g39210
ORF Names:T22F8.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G39210.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 521Glucose-1-phosphate adenylyltransferase large subunit 3, chloroplasticPRO_0000011161
Transit peptidei1 – ?ChloroplastSequence analysis

Proteomic databases

PaxDbiP55231.
PRIDEiP55231.

PTM databases

iPTMnetiP55231.

Expressioni

Tissue specificityi

Probably are expressed in roots, flowers and/or seeds.

Gene expression databases

GenevisibleiP55231. AT.

Interactioni

Subunit structurei

Heterotetramer.

Protein-protein interaction databases

STRINGi3702.AT4G39210.1.

Structurei

3D structure databases

ProteinModelPortaliP55231.
SMRiP55231. Positions 101-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IMYM. Eukaryota.
COG0448. LUCA.
HOGENOMiHOG000278604.
InParanoidiP55231.
KOiK00975.
OMAiRMIDIPM.
OrthoDBiEOG093608F5.
PhylomeDBiP55231.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005836. ADP_Glu_pyroP_CS.
IPR011831. GlgC.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
TIGRFAMsiTIGR02091. glgC. 1 hit.
PROSITEiPS00808. ADP_GLC_PYROPHOSPH_1. 1 hit.
PS00809. ADP_GLC_PYROPHOSPH_2. 1 hit.
PS00810. ADP_GLC_PYROPHOSPH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSCCNFSLG TKTVLAKDSF KNVENKFLGE KIKGSVLKPF SSDLSSKKFR
60 70 80 90 100
NRKLRPGVAY AIATSKNAKE ALKNQPSMFE RRRADPKNVA AIILGGGDGA
110 120 130 140 150
KLFPLTKRAA TPAVPVGGCY RMIDIPMSNC INSCINKIFV LTQFNSASLN
160 170 180 190 200
RHLARTYFGN GINFGDGFVE VLAATQTPGE AGKKWFQGTA DAVRKFLWVF
210 220 230 240 250
EDAKNRNIEN IIILSGDHLY RMNYMDFVQH HVDSKADITL SCAPVDESRA
260 270 280 290 300
SEYGLVNIDR SGRVVHFSEK PTGIDLKSMQ TDTTMHGLSH QEAAKSPYIA
310 320 330 340 350
SMGVYCFKTE ALLKLLTWRY PSSNDFGSEI IPAAIKDHNV QGYIYRDYWE
360 370 380 390 400
DIGTIKSFYE ANIALVEEHP KFEFYDQNTP FYTSPRFLPP TKTEKCRIVN
410 420 430 440 450
SVISHGCFLG ECSIQRSIIG ERSRLDYGVE LQDTLMLGAD SYQTESEIAS
460 470 480 490 500
LLAEGNVPIG IGRDTKIRKC IIDKNAKIGK NVVIMNKDDV KEADRPEEGF
510 520
YIRSGITVVV EKATIKDGTV I
Length:521
Mass (Da):58,029
Last modified:December 5, 2001 - v2
Checksum:i2E6AAE91F9E6E367
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti296 – 2961S → Y (PubMed:8292792).Curated
Sequence conflicti301 – 3011S → G in CAA51776 (PubMed:8292792).Curated
Sequence conflicti362 – 3621N → S in CAA51776 (PubMed:8292792).Curated
Sequence conflicti448 – 4503IAS → SR in CAA51776 (PubMed:8292792).Curated
Sequence conflicti477 – 4771K → R in CAA51776 (PubMed:8292792).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18432 Genomic DNA. Translation: CAA77173.1.
AL050351 Genomic DNA. Translation: CAB43636.1.
AL161594 Genomic DNA. Translation: CAB80584.1.
CP002687 Genomic DNA. Translation: AEE87038.1.
AY059862 mRNA. Translation: AAL24344.1.
BT010378 mRNA. Translation: AAQ56821.1.
X73364 mRNA. Translation: CAA51776.1.
PIRiT08569.
RefSeqiNP_195632.1. NM_120081.1.
UniGeneiAt.75.

Genome annotation databases

EnsemblPlantsiAT4G39210.1; AT4G39210.1; AT4G39210.
GeneIDi830076.
GrameneiAT4G39210.1; AT4G39210.1; AT4G39210.
KEGGiath:AT4G39210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18432 Genomic DNA. Translation: CAA77173.1.
AL050351 Genomic DNA. Translation: CAB43636.1.
AL161594 Genomic DNA. Translation: CAB80584.1.
CP002687 Genomic DNA. Translation: AEE87038.1.
AY059862 mRNA. Translation: AAL24344.1.
BT010378 mRNA. Translation: AAQ56821.1.
X73364 mRNA. Translation: CAA51776.1.
PIRiT08569.
RefSeqiNP_195632.1. NM_120081.1.
UniGeneiAt.75.

3D structure databases

ProteinModelPortaliP55231.
SMRiP55231. Positions 101-521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G39210.1.

PTM databases

iPTMnetiP55231.

Proteomic databases

PaxDbiP55231.
PRIDEiP55231.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G39210.1; AT4G39210.1; AT4G39210.
GeneIDi830076.
GrameneiAT4G39210.1; AT4G39210.1; AT4G39210.
KEGGiath:AT4G39210.

Organism-specific databases

TAIRiAT4G39210.

Phylogenomic databases

eggNOGiENOG410IMYM. Eukaryota.
COG0448. LUCA.
HOGENOMiHOG000278604.
InParanoidiP55231.
KOiK00975.
OMAiRMIDIPM.
OrthoDBiEOG093608F5.
PhylomeDBiP55231.

Enzyme and pathway databases

UniPathwayiUPA00152.
BRENDAi2.7.7.27. 399.
SABIO-RKP55231.

Miscellaneous databases

PROiP55231.

Gene expression databases

GenevisibleiP55231. AT.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005836. ADP_Glu_pyroP_CS.
IPR011831. GlgC.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
TIGRFAMsiTIGR02091. glgC. 1 hit.
PROSITEiPS00808. ADP_GLC_PYROPHOSPH_1. 1 hit.
PS00809. ADP_GLC_PYROPHOSPH_2. 1 hit.
PS00810. ADP_GLC_PYROPHOSPH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLGL3_ARATH
AccessioniPrimary (citable) accession number: P55231
Secondary accession number(s): Q9ZR38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 5, 2001
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.