Glucose-1-phosphate adenylyltransferase small subunit, chloroplastic precursor

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P55228 (GLGS_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glucose-1-phosphate adenylyltransferase small subunit, chloroplastic
EC=2.7.7.27

Alternative name(s):
ADP-glucose pyrophosphorylase
ADP-glucose synthase
AGPase B
Alpha-D-glucose-1-phosphate adenyl transferase

Gene names

Name:	APS1
Synonyms:	ADG1, APS
Ordered Locus Names:	At5g48300
ORF Names:	K23F3.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This protein plays a role in synthesis of starch. It catalyzes the synthesis of the activated glycosyl donor, ADP-glucose from Glc-1-P and ATP.
Catalytic activity	ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-glucose.
Enzyme regulation	Activated by 3'phosphoglycerate, inhibited by orthophosphate. Allosteric regulation.
Pathway	Glycan biosynthesis; starch biosynthesis.
Subunit structure	Heterotetramer.
Subcellular location	Plastid › chloroplast By similarity.
Tissue specificity	Leaves.
Sequence similarities	Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.

Ontologies

Keywords
Biological process	Starch biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: InterPro photoperiodism, flowering Inferred from mutant phenotype PubMed 18614708. Source: TAIR starch biosynthetic process Inferred from mutant phenotype PubMed 18614708. Source: TAIR
Cellular_component	amyloplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene apoplast Inferred from direct assay PubMed 18538804. Source: TAIR chloroplast stroma Inferred from direct assay PubMed 16207701 PubMed 18633119 PubMed 20061580. Source: TAIR cytosol Inferred from electronic annotation. Source: EnsemblPlants/Gramene heterotetrameric ADPG pyrophosphorylase complex Inferred from mutant phenotype Ref.1. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glucose-1-phosphate adenylyltransferase activity Inferred from direct assay PubMed 17452341 PubMed 18614708. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 71

Chloroplast Potential

Chain

72 – 520

449

Glucose-1-phosphate adenylyltransferase small subunit, chloroplastic

PRO_0000011149

Experimental info

Sequence conflict

194 – 195

RQ → TD in AAB09585. Ref.2

Sequence conflict

265

A → S in AAB09585. Ref.2

Sequence conflict

293 – 298

MPFIAS → YPYIAG in CAA51777. Ref.7

Sequence conflict

332 – 333

TS → PF in CAA51777. Ref.7

Sequence conflict

451

S → A in AAB09585. Ref.2

Sequence conflict

473

K → I in CAA51777. Ref.7

Sequence conflict

475

A → S in AAB09585. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P55228 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 49E8F3141A3C5C0F
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FASTA

520

56,651

        10         20         30         40         50         60 
MASVSAIGVL KVPPASTSNS TGKATEAVPT RTLSFSSSVT SSDDKISLKS TVSRLCKSVV 

        70         80         90        100        110        120 
RRNPIIVSPK AVSDSQNSQT CLDPDASSSV LGIILGGGAG TRLYPLTKKR AKPAVPLGAN 

       130        140        150        160        170        180 
YRLIDIPVSN CLNSNISKIY VLTQFNSASL NRHLSRAYAS NMGGYKNEGF VEVLAAQQSP 

       190        200        210        220        230        240 
ENPNWFQGTA DAVRQYLWLF EEHNVLEYLI LAGDHLYRMD YEKFIQAHRE TDADITVAAL 

       250        260        270        280        290        300 
PMDEQRATAF GLMKIDEEGR IIEFAEKPKG EHLKAMKVDT TILGLDDQRA KEMPFIASMG 

       310        320        330        340        350        360 
IYVVSRDVML DLLRNQFPGA NDFGSEVIPG ATSLGLRVQA YLYDGYWEDI GTIEAFYNAN 

       370        380        390        400        410        420 
LGITKKPVPD FSFYDRSAPI YTQPRYLPPS KMLDADVTDS VIGEGCVIKN CKIHHSVVGL 

       430        440        450        460        470        480 
RSCISEGAII EDSLLMGADY YETATEKSLL SAKGSVPIGI GKNSHIKRAI IDKNARIGDN 

       490        500        510        520 
VKIINSDNVQ EAARETDGYF IKSGIVTVIK DALIPTGTVI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of ADG1, an Arabidopsis locus encoding for ADPG pyrophosphorylase small subunit, demonstrates that the presence of the small subunit is required for large subunit stability." Wang S.-M., Lue W.-L., Yu T.-S., Long J.-H., Wang C.-N., Eimert K., Chen J. Plant J. 13:63-70(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"cDNA cloning of ADP glucose pyrophosphorylase small subunit in Arabidopsis thaliana." Choi S.B., Okita T.W. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"Isolation of ADPglucose pyrophosphorylase small subunit gene from Arabidopsis thaliana." Ito H., Kato C., Matsui H., Okita T.W. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Molecular characterization of multiple cDNA clones for ADP-glucose pyrophosphorylase from Arabidopsis thaliana." Villand P., Olsen O.-A., Kleczkowski L.A. Plant Mol. Biol. 23:1279-1284(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 293-478. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U72351 mRNA. Translation: AAC39441.1. U70616 mRNA. Translation: AAB09585.1. AB039889 Genomic DNA. Translation: BAA92523.1. AP000372 Genomic DNA. Translation: BAA98187.1. CP002688 Genomic DNA. Translation: AED95648.1. AY049265 mRNA. Translation: AAK83607.1. AY065428 mRNA. Translation: AAL38869.1. AY090283 mRNA. Translation: AAL90944.1. AY096379 mRNA. Translation: AAM20020.1. X73365 mRNA. Translation: CAA51777.1.
IPI	IPI00542391.
RefSeq	NP_199641.1. NM_124205.3.
UniGene	At.670.
3D structure databases
ProteinModelPortal	P55228.
SMR	P55228. Positions 79-520.
ModBase	Search...
Protein-protein interaction databases
IntAct	P55228. 2 interactions.
Proteomic databases
PaxDb	P55228.
PRIDE	P55228.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G48300.1; AT5G48300.1; AT5G48300.
GeneID	834883.
KEGG	ath:AT5G48300.
Organism-specific databases
TAIR	At5g48300.
Phylogenomic databases
eggNOG	COG0448.
HOGENOM	HOG000278604.
InParanoid	P55228.
KO	K00975.
OMA	DEESAHD.
PhylomeDB	P55228.
ProtClustDB	PLN02241.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-1822.
BRENDA	2.7.7.27. 399.
UniPathway	UPA00152.
Gene expression databases
ArrayExpress	P55228.
Genevestigator	P55228.
GermOnline	AT5G48300. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005836. ADP_Glu_pyroP_CS. IPR011831. GlgC. IPR005835. NTP_transferase. IPR011004. Trimer_LpxA-like. [Graphical view]
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR02091. glgC. 1 hit.
PROSITE	PS00808. ADP_GLC_PYROPHOSPH_1. 1 hit. PS00809. ADP_GLC_PYROPHOSPH_2. 1 hit. PS00810. ADP_GLC_PYROPHOSPH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLGS_ARATH

Accession

Primary (citable) accession number: P55228
Secondary accession number(s): O64400, Q96534

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents