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Protein

Glucose-1-phosphate adenylyltransferase small subunit, chloroplastic

Gene

APS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This protein plays a role in synthesis of starch. It catalyzes the synthesis of the activated glycosyl donor, ADP-glucose from Glc-1-P and ATP.

Catalytic activityi

ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-glucose.

Enzyme regulationi

Activated by 3'phosphoglycerate, inhibited by orthophosphate. Allosteric regulation.

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glucose-1-phosphate adenylyltransferase activity Source: TAIR

GO - Biological processi

  • glycogen biosynthetic process Source: InterPro
  • photoperiodism, flowering Source: TAIR
  • starch biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Starch biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-1822.
SABIO-RKP55228.
UniPathwayiUPA00152.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-1-phosphate adenylyltransferase small subunit, chloroplastic (EC:2.7.7.27)
Alternative name(s):
ADP-glucose pyrophosphorylase
ADP-glucose synthase
AGPase B
Alpha-D-glucose-1-phosphate adenyl transferase
Gene namesi
Name:APS1
Synonyms:ADG1, APS
Ordered Locus Names:At5g48300
ORF Names:K23F3.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G48300.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • heterotetrameric ADPG pyrophosphorylase complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7171ChloroplastSequence analysisAdd
BLAST
Chaini72 – 520449Glucose-1-phosphate adenylyltransferase small subunit, chloroplasticPRO_0000011149Add
BLAST

Proteomic databases

PaxDbiP55228.
PRIDEiP55228.

PTM databases

iPTMnetiP55228.

Expressioni

Tissue specificityi

Leaves.

Gene expression databases

GenevisibleiP55228. AT.

Interactioni

Subunit structurei

Heterotetramer.

Protein-protein interaction databases

BioGridi20129. 4 interactions.
IntActiP55228. 2 interactions.
STRINGi3702.AT5G48300.1.

Structurei

3D structure databases

ProteinModelPortaliP55228.
SMRiP55228. Positions 79-520.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IMYM. Eukaryota.
COG0448. LUCA.
HOGENOMiHOG000278604.
InParanoidiP55228.
KOiK00975.
OMAiFGCIDSD.
PhylomeDBiP55228.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005836. ADP_Glu_pyroP_CS.
IPR011831. GlgC.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
TIGRFAMsiTIGR02091. glgC. 1 hit.
PROSITEiPS00808. ADP_GLC_PYROPHOSPH_1. 1 hit.
PS00809. ADP_GLC_PYROPHOSPH_2. 1 hit.
PS00810. ADP_GLC_PYROPHOSPH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVSAIGVL KVPPASTSNS TGKATEAVPT RTLSFSSSVT SSDDKISLKS
60 70 80 90 100
TVSRLCKSVV RRNPIIVSPK AVSDSQNSQT CLDPDASSSV LGIILGGGAG
110 120 130 140 150
TRLYPLTKKR AKPAVPLGAN YRLIDIPVSN CLNSNISKIY VLTQFNSASL
160 170 180 190 200
NRHLSRAYAS NMGGYKNEGF VEVLAAQQSP ENPNWFQGTA DAVRQYLWLF
210 220 230 240 250
EEHNVLEYLI LAGDHLYRMD YEKFIQAHRE TDADITVAAL PMDEQRATAF
260 270 280 290 300
GLMKIDEEGR IIEFAEKPKG EHLKAMKVDT TILGLDDQRA KEMPFIASMG
310 320 330 340 350
IYVVSRDVML DLLRNQFPGA NDFGSEVIPG ATSLGLRVQA YLYDGYWEDI
360 370 380 390 400
GTIEAFYNAN LGITKKPVPD FSFYDRSAPI YTQPRYLPPS KMLDADVTDS
410 420 430 440 450
VIGEGCVIKN CKIHHSVVGL RSCISEGAII EDSLLMGADY YETATEKSLL
460 470 480 490 500
SAKGSVPIGI GKNSHIKRAI IDKNARIGDN VKIINSDNVQ EAARETDGYF
510 520
IKSGIVTVIK DALIPTGTVI
Length:520
Mass (Da):56,651
Last modified:December 1, 2000 - v2
Checksum:i49E8F3141A3C5C0F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti194 – 1952RQ → TD in AAB09585 (Ref. 2) Curated
Sequence conflicti265 – 2651A → S in AAB09585 (Ref. 2) Curated
Sequence conflicti293 – 2986MPFIAS → YPYIAG in CAA51777 (PubMed:8292792).Curated
Sequence conflicti332 – 3332TS → PF in CAA51777 (PubMed:8292792).Curated
Sequence conflicti451 – 4511S → A in AAB09585 (Ref. 2) Curated
Sequence conflicti473 – 4731K → I in CAA51777 (PubMed:8292792).Curated
Sequence conflicti475 – 4751A → S in AAB09585 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72351 mRNA. Translation: AAC39441.1.
U70616 mRNA. Translation: AAB09585.1.
AB039889 Genomic DNA. Translation: BAA92523.1.
AP000372 Genomic DNA. Translation: BAA98187.1.
CP002688 Genomic DNA. Translation: AED95648.1.
AY049265 mRNA. Translation: AAK83607.1.
AY065428 mRNA. Translation: AAL38869.1.
AY090283 mRNA. Translation: AAL90944.1.
AY096379 mRNA. Translation: AAM20020.1.
X73365 mRNA. Translation: CAA51777.1.
RefSeqiNP_199641.1. NM_124205.3.
UniGeneiAt.670.

Genome annotation databases

EnsemblPlantsiAT5G48300.1; AT5G48300.1; AT5G48300.
GeneIDi834883.
GrameneiAT5G48300.1; AT5G48300.1; AT5G48300.
KEGGiath:AT5G48300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72351 mRNA. Translation: AAC39441.1.
U70616 mRNA. Translation: AAB09585.1.
AB039889 Genomic DNA. Translation: BAA92523.1.
AP000372 Genomic DNA. Translation: BAA98187.1.
CP002688 Genomic DNA. Translation: AED95648.1.
AY049265 mRNA. Translation: AAK83607.1.
AY065428 mRNA. Translation: AAL38869.1.
AY090283 mRNA. Translation: AAL90944.1.
AY096379 mRNA. Translation: AAM20020.1.
X73365 mRNA. Translation: CAA51777.1.
RefSeqiNP_199641.1. NM_124205.3.
UniGeneiAt.670.

3D structure databases

ProteinModelPortaliP55228.
SMRiP55228. Positions 79-520.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20129. 4 interactions.
IntActiP55228. 2 interactions.
STRINGi3702.AT5G48300.1.

PTM databases

iPTMnetiP55228.

Proteomic databases

PaxDbiP55228.
PRIDEiP55228.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G48300.1; AT5G48300.1; AT5G48300.
GeneIDi834883.
GrameneiAT5G48300.1; AT5G48300.1; AT5G48300.
KEGGiath:AT5G48300.

Organism-specific databases

TAIRiAT5G48300.

Phylogenomic databases

eggNOGiENOG410IMYM. Eukaryota.
COG0448. LUCA.
HOGENOMiHOG000278604.
InParanoidiP55228.
KOiK00975.
OMAiFGCIDSD.
PhylomeDBiP55228.

Enzyme and pathway databases

UniPathwayiUPA00152.
BioCyciMetaCyc:MONOMER-1822.
SABIO-RKP55228.

Miscellaneous databases

PROiP55228.

Gene expression databases

GenevisibleiP55228. AT.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005836. ADP_Glu_pyroP_CS.
IPR011831. GlgC.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
TIGRFAMsiTIGR02091. glgC. 1 hit.
PROSITEiPS00808. ADP_GLC_PYROPHOSPH_1. 1 hit.
PS00809. ADP_GLC_PYROPHOSPH_2. 1 hit.
PS00810. ADP_GLC_PYROPHOSPH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of ADG1, an Arabidopsis locus encoding for ADPG pyrophosphorylase small subunit, demonstrates that the presence of the small subunit is required for large subunit stability."
    Wang S.-M., Lue W.-L., Yu T.-S., Long J.-H., Wang C.-N., Eimert K., Chen J.
    Plant J. 13:63-70(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "cDNA cloning of ADP glucose pyrophosphorylase small subunit in Arabidopsis thaliana."
    Choi S.B., Okita T.W.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. "Isolation of ADPglucose pyrophosphorylase small subunit gene from Arabidopsis thaliana."
    Ito H., Kato C., Matsui H., Okita T.W.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  4. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
    Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Molecular characterization of multiple cDNA clones for ADP-glucose pyrophosphorylase from Arabidopsis thaliana."
    Villand P., Olsen O.-A., Kleczkowski L.A.
    Plant Mol. Biol. 23:1279-1284(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 293-478.
    Strain: cv. Columbia.

Entry informationi

Entry nameiGLGS_ARATH
AccessioniPrimary (citable) accession number: P55228
Secondary accession number(s): O64400, Q96534
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: February 17, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.