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Protein

Glucose-1-phosphate adenylyltransferase small subunit, chloroplastic

Gene

APS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

This protein plays a role in synthesis of starch. It catalyzes the synthesis of the activated glycosyl donor, ADP-glucose from Glc-1-P and ATP.

Catalytic activityi

ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-glucose.

Enzyme regulationi

Activated by 3'phosphoglycerate, inhibited by orthophosphate. Allosteric regulation.

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glucose-1-phosphate adenylyltransferase activity Source: TAIR

GO - Biological processi

  • glycogen biosynthetic process Source: InterPro
  • photoperiodism, flowering Source: TAIR
  • starch biosynthetic process Source: TAIR

Keywordsi

Molecular functionAllosteric enzyme, Nucleotidyltransferase, Transferase
Biological processStarch biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:MONOMER-1822
MetaCyc:MONOMER-1822
SABIO-RKiP55228
UniPathwayiUPA00152

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-1-phosphate adenylyltransferase small subunit, chloroplastic (EC:2.7.7.27)
Alternative name(s):
ADP-glucose pyrophosphorylase
ADP-glucose synthase
AGPase B
Alpha-D-glucose-1-phosphate adenyl transferase
Gene namesi
Name:APS1
Synonyms:ADG1, APS
Ordered Locus Names:At5g48300
ORF Names:K23F3.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G48300
TAIRilocus:2156263 AT5G48300

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 71ChloroplastSequence analysisAdd BLAST71
ChainiPRO_000001114972 – 520Glucose-1-phosphate adenylyltransferase small subunit, chloroplasticAdd BLAST449

Proteomic databases

PaxDbiP55228
PRIDEiP55228

PTM databases

iPTMnetiP55228

Expressioni

Tissue specificityi

Leaves.

Gene expression databases

ExpressionAtlasiP55228 baseline and differential
GenevisibleiP55228 AT

Interactioni

Subunit structurei

Heterotetramer.

Protein-protein interaction databases

BioGridi20129, 4 interactors
IntActiP55228, 2 interactors
STRINGi3702.AT5G48300.1

Structurei

3D structure databases

ProteinModelPortaliP55228
SMRiP55228
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IMYM Eukaryota
COG0448 LUCA
HOGENOMiHOG000278604
InParanoidiP55228
KOiK00975
OMAiQLQAMKV
OrthoDBiEOG093606WL
PhylomeDBiP55228

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR011831 ADP-Glc_PPase
IPR005836 ADP_Glu_pyroP_CS
IPR005835 NTP_transferase_dom
IPR029044 Nucleotide-diphossugar_trans
IPR011004 Trimer_LpxA-like_sf
PfamiView protein in Pfam
PF00483 NTP_transferase, 1 hit
SUPFAMiSSF51161 SSF51161, 1 hit
SSF53448 SSF53448, 2 hits
TIGRFAMsiTIGR02091 glgC, 1 hit
PROSITEiView protein in PROSITE
PS00808 ADP_GLC_PYROPHOSPH_1, 1 hit
PS00809 ADP_GLC_PYROPHOSPH_2, 1 hit
PS00810 ADP_GLC_PYROPHOSPH_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVSAIGVL KVPPASTSNS TGKATEAVPT RTLSFSSSVT SSDDKISLKS
60 70 80 90 100
TVSRLCKSVV RRNPIIVSPK AVSDSQNSQT CLDPDASSSV LGIILGGGAG
110 120 130 140 150
TRLYPLTKKR AKPAVPLGAN YRLIDIPVSN CLNSNISKIY VLTQFNSASL
160 170 180 190 200
NRHLSRAYAS NMGGYKNEGF VEVLAAQQSP ENPNWFQGTA DAVRQYLWLF
210 220 230 240 250
EEHNVLEYLI LAGDHLYRMD YEKFIQAHRE TDADITVAAL PMDEQRATAF
260 270 280 290 300
GLMKIDEEGR IIEFAEKPKG EHLKAMKVDT TILGLDDQRA KEMPFIASMG
310 320 330 340 350
IYVVSRDVML DLLRNQFPGA NDFGSEVIPG ATSLGLRVQA YLYDGYWEDI
360 370 380 390 400
GTIEAFYNAN LGITKKPVPD FSFYDRSAPI YTQPRYLPPS KMLDADVTDS
410 420 430 440 450
VIGEGCVIKN CKIHHSVVGL RSCISEGAII EDSLLMGADY YETATEKSLL
460 470 480 490 500
SAKGSVPIGI GKNSHIKRAI IDKNARIGDN VKIINSDNVQ EAARETDGYF
510 520
IKSGIVTVIK DALIPTGTVI
Length:520
Mass (Da):56,651
Last modified:December 1, 2000 - v2
Checksum:i49E8F3141A3C5C0F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194 – 195RQ → TD in AAB09585 (Ref. 2) Curated2
Sequence conflicti265A → S in AAB09585 (Ref. 2) Curated1
Sequence conflicti293 – 298MPFIAS → YPYIAG in CAA51777 (PubMed:8292792).Curated6
Sequence conflicti332 – 333TS → PF in CAA51777 (PubMed:8292792).Curated2
Sequence conflicti451S → A in AAB09585 (Ref. 2) Curated1
Sequence conflicti473K → I in CAA51777 (PubMed:8292792).Curated1
Sequence conflicti475A → S in AAB09585 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72351 mRNA Translation: AAC39441.1
U70616 mRNA Translation: AAB09585.1
AB039889 Genomic DNA Translation: BAA92523.1
AP000372 Genomic DNA Translation: BAA98187.1
CP002688 Genomic DNA Translation: AED95648.1
AY049265 mRNA Translation: AAK83607.1
AY065428 mRNA Translation: AAL38869.1
AY090283 mRNA Translation: AAL90944.1
AY096379 mRNA Translation: AAM20020.1
X73365 mRNA Translation: CAA51777.1
RefSeqiNP_199641.1, NM_124205.4
UniGeneiAt.670

Genome annotation databases

EnsemblPlantsiAT5G48300.1; AT5G48300.1; AT5G48300
GeneIDi834883
GrameneiAT5G48300.1; AT5G48300.1; AT5G48300
KEGGiath:AT5G48300

Similar proteinsi

Entry informationi

Entry nameiGLGS_ARATH
AccessioniPrimary (citable) accession number: P55228
Secondary accession number(s): O64400, Q96534
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: April 25, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health