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Protein

Cyclic AMP receptor-like protein

Gene

vfr

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei133cAMPCurated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 137cAMPBy similarityAdd BLAST127
DNA bindingi174 – 193H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • pathogenesis Source: PseudoCAP
  • positive regulation of multi-organism process Source: PseudoCAP
  • transcription, DNA-templated Source: CACAO
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Virulence

Keywords - Ligandi

cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP receptor-like protein
Gene namesi
Name:vfr
Ordered Locus Names:PA0652
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0652.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001001891 – 214Cyclic AMP receptor-like proteinAdd BLAST214

Proteomic databases

PaxDbiP55222.
PRIDEiP55222.

Expressioni

Gene expression databases

CollecTFiEXPREG_00000b50.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0652.

Structurei

Secondary structure

1214
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 18Combined sources9
Beta strandi19 – 25Combined sources7
Beta strandi30 – 32Combined sources3
Beta strandi40 – 54Combined sources15
Beta strandi60 – 67Combined sources8
Beta strandi71 – 73Combined sources3
Beta strandi89 – 95Combined sources7
Beta strandi97 – 103Combined sources7
Helixi104 – 113Combined sources10
Helixi115 – 141Combined sources27
Helixi144 – 155Combined sources12
Beta strandi162 – 164Combined sources3
Beta strandi167 – 171Combined sources5
Helixi174 – 181Combined sources8
Helixi185 – 197Combined sources13
Beta strandi200 – 204Combined sources5
Beta strandi207 – 211Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OZ6X-ray2.80A9-214[»]
ProteinModelPortaliP55222.
SMRiP55222.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55222.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini142 – 214HTH crp-typePROSITE-ProRule annotationAdd BLAST73

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107RYR. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000250565.
InParanoidiP55222.
KOiK10914.
OMAiKTMVVYG.
PhylomeDBiP55222.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAITHTPKL KHLDKLLAHC HRRRYTAKST IIYAGDRCET LFFIIKGSVT
60 70 80 90 100
ILIEDDDGRE MIIGYLNSGD FFGELGLFEK EGSEQERSAW VRAKVECEVA
110 120 130 140 150
EISYAKFREL SQQDSEILYT LGSQMADRLR KTTRKVGDLA FLDVTGRVAR
160 170 180 190 200
TLLDLCQQPD AMTHPDGMQI KITRQEIGRI VGCSREMVGR VLKSLEEQGL
210
VHVKGKTMVV FGTR
Length:214
Mass (Da):24,225
Last modified:October 1, 1996 - v1
Checksum:i03B7F2A4C2CED7E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16318 Genomic DNA. Translation: AAA73464.1.
AE004091 Genomic DNA. Translation: AAG04041.1.
PIRiA55540.
RefSeqiNP_249343.1. NC_002516.2.
WP_003085214.1. NZ_ASJY01000103.1.

Genome annotation databases

EnsemblBacteriaiAAG04041; AAG04041; PA0652.
GeneIDi880744.
KEGGipae:PA0652.
PATRICi19835566. VBIPseAer58763_0683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16318 Genomic DNA. Translation: AAA73464.1.
AE004091 Genomic DNA. Translation: AAG04041.1.
PIRiA55540.
RefSeqiNP_249343.1. NC_002516.2.
WP_003085214.1. NZ_ASJY01000103.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OZ6X-ray2.80A9-214[»]
ProteinModelPortaliP55222.
SMRiP55222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0652.

Proteomic databases

PaxDbiP55222.
PRIDEiP55222.

Protocols and materials databases

DNASUi880744.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04041; AAG04041; PA0652.
GeneIDi880744.
KEGGipae:PA0652.
PATRICi19835566. VBIPseAer58763_0683.

Organism-specific databases

PseudoCAPiPA0652.

Phylogenomic databases

eggNOGiENOG4107RYR. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000250565.
InParanoidiP55222.
KOiK10914.
OMAiKTMVVYG.
PhylomeDBiP55222.

Miscellaneous databases

EvolutionaryTraceiP55222.

Gene expression databases

CollecTFiEXPREG_00000b50.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVFR_PSEAE
AccessioniPrimary (citable) accession number: P55222
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.