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P55222 (VFR_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cyclic AMP receptor-like protein
Gene names
Name:vfr
Ordered Locus Names:PA0652
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production.

Sequence similarities

Contains 1 cyclic nucleotide-binding domain.

Contains 1 HTH crp-type DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 214214Cyclic AMP receptor-like protein
PRO_0000100189

Regions

Domain142 – 21473HTH crp-type
Nucleotide binding11 – 137127cAMP By similarity
DNA binding174 – 19320H-T-H motif By similarity

Sites

Binding site1331cAMP Probable

Secondary structure

................................. 214
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P55222 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 03B7F2A4C2CED7E6

FASTA21424,225
        10         20         30         40         50         60 
MVAITHTPKL KHLDKLLAHC HRRRYTAKST IIYAGDRCET LFFIIKGSVT ILIEDDDGRE 

        70         80         90        100        110        120 
MIIGYLNSGD FFGELGLFEK EGSEQERSAW VRAKVECEVA EISYAKFREL SQQDSEILYT 

       130        140        150        160        170        180 
LGSQMADRLR KTTRKVGDLA FLDVTGRVAR TLLDLCQQPD AMTHPDGMQI KITRQEIGRI 

       190        200        210 
VGCSREMVGR VLKSLEEQGL VHVKGKTMVV FGTR 

« Hide

References

« Hide 'large scale' references
[1]"The vfr gene product, required for Pseudomonas aeruginosa exotoxin A and protease production, belongs to the cyclic AMP receptor protein family."
West S.E.H., Sample A.K., Runyen-Janecky L.J.
J. Bacteriol. 176:7532-7542(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U16318 Genomic DNA. Translation: AAA73464.1.
AE004091 Genomic DNA. Translation: AAG04041.1.
PIRA55540.
RefSeqNP_249343.1. NC_002516.2.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2OZ6X-ray2.80A9-214[»]
ProteinModelPortalP55222.
SMRP55222. Positions 9-213.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA0652.

Protocols and materials databases

DNASU880744.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG04041; AAG04041; PA0652.
GeneID880744.
KEGGpae:PA0652.
PATRIC19835566. VBIPseAer58763_0683.

Organism-specific databases

PseudoCAPPA0652.

Phylogenomic databases

eggNOGCOG0664.
HOGENOMHOG000250565.
KOK10914.
OMALGSQMAE.
OrthoDBEOG69GZGV.
PhylomeDBP55222.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSPR00034. HTHCRP.
SMARTSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
PROSITEPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP55222.

Entry information

Entry nameVFR_PSEAE
AccessionPrimary (citable) accession number: P55222
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references