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Protein

Cyclic AMP receptor-like protein

Gene

vfr

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei133cAMPCurated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 137cAMPBy similarityAdd BLAST127
DNA bindingi174 – 193H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • pathogenesis Source: PseudoCAP
  • positive regulation of multi-organism process Source: PseudoCAP
  • positive regulation of transcription, DNA-templated Source: CollecTF
  • transcription, DNA-templated Source: CACAO

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation, Virulence
LigandcAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-657-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP receptor-like protein
Gene namesi
Name:vfr
Ordered Locus Names:PA0652
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0652

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: InterPro
  • protein-DNA complex Source: CollecTF

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001001891 – 214Cyclic AMP receptor-like proteinAdd BLAST214

Proteomic databases

PaxDbiP55222

Expressioni

Gene expression databases

CollecTFiEXPREG_00000b50

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0652

Structurei

Secondary structure

1214
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 18Combined sources9
Beta strandi19 – 25Combined sources7
Beta strandi30 – 32Combined sources3
Beta strandi40 – 54Combined sources15
Beta strandi60 – 67Combined sources8
Beta strandi71 – 73Combined sources3
Beta strandi89 – 95Combined sources7
Beta strandi97 – 103Combined sources7
Helixi104 – 113Combined sources10
Helixi115 – 141Combined sources27
Helixi144 – 155Combined sources12
Beta strandi162 – 164Combined sources3
Beta strandi167 – 171Combined sources5
Helixi174 – 181Combined sources8
Helixi185 – 197Combined sources13
Beta strandi200 – 204Combined sources5
Beta strandi207 – 211Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OZ6X-ray2.80A9-214[»]
ProteinModelPortaliP55222
SMRiP55222
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55222

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini142 – 214HTH crp-typePROSITE-ProRule annotationAdd BLAST73

Phylogenomic databases

eggNOGiENOG4107RYR Bacteria
COG0664 LUCA
HOGENOMiHOG000250565
InParanoidiP55222
KOiK10914
OMAiTECEVAE
PhylomeDBiP55222

Family and domain databases

CDDicd00038 CAP_ED, 1 hit
Gene3Di1.10.10.10, 1 hit
2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR012318 HTH_CRP
IPR014710 RmlC-like_jellyroll
IPR018335 Tscrpt_reg_HTH_Crp-type_CS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00027 cNMP_binding, 1 hit
PF00325 Crp, 1 hit
PRINTSiPR00034 HTHCRP
SMARTiView protein in SMART
SM00100 cNMP, 1 hit
SM00419 HTH_CRP, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF51206 SSF51206, 1 hit
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 1 hit
PS00889 CNMP_BINDING_2, 1 hit
PS50042 CNMP_BINDING_3, 1 hit
PS00042 HTH_CRP_1, 1 hit
PS51063 HTH_CRP_2, 1 hit

Sequencei

Sequence statusi: Complete.

P55222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAITHTPKL KHLDKLLAHC HRRRYTAKST IIYAGDRCET LFFIIKGSVT
60 70 80 90 100
ILIEDDDGRE MIIGYLNSGD FFGELGLFEK EGSEQERSAW VRAKVECEVA
110 120 130 140 150
EISYAKFREL SQQDSEILYT LGSQMADRLR KTTRKVGDLA FLDVTGRVAR
160 170 180 190 200
TLLDLCQQPD AMTHPDGMQI KITRQEIGRI VGCSREMVGR VLKSLEEQGL
210
VHVKGKTMVV FGTR
Length:214
Mass (Da):24,225
Last modified:October 1, 1996 - v1
Checksum:i03B7F2A4C2CED7E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16318 Genomic DNA Translation: AAA73464.1
AE004091 Genomic DNA Translation: AAG04041.1
PIRiA55540
RefSeqiNP_249343.1, NC_002516.2
WP_003085214.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG04041; AAG04041; PA0652
GeneIDi880744
KEGGipae:PA0652
PATRICifig|208964.12.peg.683

Similar proteinsi

Entry informationi

Entry nameiVFR_PSEAE
AccessioniPrimary (citable) accession number: P55222
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health