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P55222

- VFR_PSEAE

UniProt

P55222 - VFR_PSEAE

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Protein

Cyclic AMP receptor-like protein

Gene
vfr, PA0652
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei133 – 1331cAMP Inferred

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 137127cAMP By similarityAdd
BLAST
DNA bindingi174 – 19320H-T-H motif By similarityAdd
BLAST

GO - Molecular functioni

  1. cAMP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. pathogenesis Source: PseudoCAP
  2. positive regulation of multi-organism process Source: PseudoCAP
  3. transcription, DNA-templated Source: CACAO
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Virulence

Keywords - Ligandi

cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP receptor-like protein
Gene namesi
Name:vfr
Ordered Locus Names:PA0652
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA0652.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 214214Cyclic AMP receptor-like proteinPRO_0000100189Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0652.

Structurei

Secondary structure

1
214
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 189
Beta strandi19 – 257
Beta strandi30 – 323
Beta strandi40 – 5415
Beta strandi60 – 678
Beta strandi71 – 733
Beta strandi89 – 957
Beta strandi97 – 1037
Helixi104 – 11310
Helixi115 – 14127
Helixi144 – 15512
Beta strandi162 – 1643
Beta strandi167 – 1715
Helixi174 – 1818
Helixi185 – 19713
Beta strandi200 – 2045
Beta strandi207 – 2115

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OZ6X-ray2.80A9-214[»]
ProteinModelPortaliP55222.
SMRiP55222. Positions 9-213.

Miscellaneous databases

EvolutionaryTraceiP55222.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini142 – 21473HTH crp-typeAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000250565.
KOiK10914.
OMAiLGSQMAE.
OrthoDBiEOG69GZGV.
PhylomeDBiP55222.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55222-1 [UniParc]FASTAAdd to Basket

« Hide

MVAITHTPKL KHLDKLLAHC HRRRYTAKST IIYAGDRCET LFFIIKGSVT    50
ILIEDDDGRE MIIGYLNSGD FFGELGLFEK EGSEQERSAW VRAKVECEVA 100
EISYAKFREL SQQDSEILYT LGSQMADRLR KTTRKVGDLA FLDVTGRVAR 150
TLLDLCQQPD AMTHPDGMQI KITRQEIGRI VGCSREMVGR VLKSLEEQGL 200
VHVKGKTMVV FGTR 214
Length:214
Mass (Da):24,225
Last modified:October 1, 1996 - v1
Checksum:i03B7F2A4C2CED7E6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U16318 Genomic DNA. Translation: AAA73464.1.
AE004091 Genomic DNA. Translation: AAG04041.1.
PIRiA55540.
RefSeqiNP_249343.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04041; AAG04041; PA0652.
GeneIDi880744.
KEGGipae:PA0652.
PATRICi19835566. VBIPseAer58763_0683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U16318 Genomic DNA. Translation: AAA73464.1 .
AE004091 Genomic DNA. Translation: AAG04041.1 .
PIRi A55540.
RefSeqi NP_249343.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2OZ6 X-ray 2.80 A 9-214 [» ]
ProteinModelPortali P55222.
SMRi P55222. Positions 9-213.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA0652.

Protocols and materials databases

DNASUi 880744.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG04041 ; AAG04041 ; PA0652 .
GeneIDi 880744.
KEGGi pae:PA0652.
PATRICi 19835566. VBIPseAer58763_0683.

Organism-specific databases

PseudoCAPi PA0652.

Phylogenomic databases

eggNOGi COG0664.
HOGENOMi HOG000250565.
KOi K10914.
OMAi LGSQMAE.
OrthoDBi EOG69GZGV.
PhylomeDBi P55222.

Miscellaneous databases

EvolutionaryTracei P55222.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProi IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view ]
PRINTSi PR00034. HTHCRP.
SMARTi SM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The vfr gene product, required for Pseudomonas aeruginosa exotoxin A and protease production, belongs to the cyclic AMP receptor protein family."
    West S.E.H., Sample A.K., Runyen-Janecky L.J.
    J. Bacteriol. 176:7532-7542(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiVFR_PSEAE
AccessioniPrimary (citable) accession number: P55222
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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