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P55222

- VFR_PSEAE

UniProt

P55222 - VFR_PSEAE

Protein

Cyclic AMP receptor-like protein

Gene

vfr

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei133 – 1331cAMPCurated

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 137127cAMPBy similarityAdd
    BLAST
    DNA bindingi174 – 19320H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cAMP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. pathogenesis Source: PseudoCAP
    2. positive regulation of multi-organism process Source: PseudoCAP
    3. transcription, DNA-templated Source: CACAO

    Keywords - Biological processi

    Transcription, Transcription regulation, Virulence

    Keywords - Ligandi

    cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclic AMP receptor-like protein
    Gene namesi
    Name:vfr
    Ordered Locus Names:PA0652
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA0652.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 214214Cyclic AMP receptor-like proteinPRO_0000100189Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi208964.PA0652.

    Structurei

    Secondary structure

    1
    214
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi10 – 189
    Beta strandi19 – 257
    Beta strandi30 – 323
    Beta strandi40 – 5415
    Beta strandi60 – 678
    Beta strandi71 – 733
    Beta strandi89 – 957
    Beta strandi97 – 1037
    Helixi104 – 11310
    Helixi115 – 14127
    Helixi144 – 15512
    Beta strandi162 – 1643
    Beta strandi167 – 1715
    Helixi174 – 1818
    Helixi185 – 19713
    Beta strandi200 – 2045
    Beta strandi207 – 2115

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2OZ6X-ray2.80A9-214[»]
    ProteinModelPortaliP55222.
    SMRiP55222. Positions 9-213.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP55222.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini142 – 21473HTH crp-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0664.
    HOGENOMiHOG000250565.
    KOiK10914.
    OMAiLGSQMAE.
    OrthoDBiEOG69GZGV.
    PhylomeDBiP55222.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view]
    PRINTSiPR00034. HTHCRP.
    SMARTiSM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P55222-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAITHTPKL KHLDKLLAHC HRRRYTAKST IIYAGDRCET LFFIIKGSVT    50
    ILIEDDDGRE MIIGYLNSGD FFGELGLFEK EGSEQERSAW VRAKVECEVA 100
    EISYAKFREL SQQDSEILYT LGSQMADRLR KTTRKVGDLA FLDVTGRVAR 150
    TLLDLCQQPD AMTHPDGMQI KITRQEIGRI VGCSREMVGR VLKSLEEQGL 200
    VHVKGKTMVV FGTR 214
    Length:214
    Mass (Da):24,225
    Last modified:October 1, 1996 - v1
    Checksum:i03B7F2A4C2CED7E6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16318 Genomic DNA. Translation: AAA73464.1.
    AE004091 Genomic DNA. Translation: AAG04041.1.
    PIRiA55540.
    RefSeqiNP_249343.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG04041; AAG04041; PA0652.
    GeneIDi880744.
    KEGGipae:PA0652.
    PATRICi19835566. VBIPseAer58763_0683.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16318 Genomic DNA. Translation: AAA73464.1 .
    AE004091 Genomic DNA. Translation: AAG04041.1 .
    PIRi A55540.
    RefSeqi NP_249343.1. NC_002516.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2OZ6 X-ray 2.80 A 9-214 [» ]
    ProteinModelPortali P55222.
    SMRi P55222. Positions 9-213.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA0652.

    Protocols and materials databases

    DNASUi 880744.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG04041 ; AAG04041 ; PA0652 .
    GeneIDi 880744.
    KEGGi pae:PA0652.
    PATRICi 19835566. VBIPseAer58763_0683.

    Organism-specific databases

    PseudoCAPi PA0652.

    Phylogenomic databases

    eggNOGi COG0664.
    HOGENOMi HOG000250565.
    KOi K10914.
    OMAi LGSQMAE.
    OrthoDBi EOG69GZGV.
    PhylomeDBi P55222.

    Miscellaneous databases

    EvolutionaryTracei P55222.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view ]
    PRINTSi PR00034. HTHCRP.
    SMARTi SM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The vfr gene product, required for Pseudomonas aeruginosa exotoxin A and protease production, belongs to the cyclic AMP receptor protein family."
      West S.E.H., Sample A.K., Runyen-Janecky L.J.
      J. Bacteriol. 176:7532-7542(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiVFR_PSEAE
    AccessioniPrimary (citable) accession number: P55222
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3