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Protein

Cyclic AMP receptor-like protein

Gene

vfr

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei133 – 1331cAMPCurated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 137127cAMPBy similarityAdd
BLAST
DNA bindingi174 – 19320H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cAMP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. pathogenesis Source: PseudoCAP
  2. positive regulation of multi-organism process Source: PseudoCAP
  3. transcription, DNA-templated Source: CACAO
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Virulence

Keywords - Ligandi

cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP receptor-like protein
Gene namesi
Name:vfr
Ordered Locus Names:PA0652
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA0652.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 214214Cyclic AMP receptor-like proteinPRO_0000100189Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0652.

Structurei

Secondary structure

1
214
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 189Combined sources
Beta strandi19 – 257Combined sources
Beta strandi30 – 323Combined sources
Beta strandi40 – 5415Combined sources
Beta strandi60 – 678Combined sources
Beta strandi71 – 733Combined sources
Beta strandi89 – 957Combined sources
Beta strandi97 – 1037Combined sources
Helixi104 – 11310Combined sources
Helixi115 – 14127Combined sources
Helixi144 – 15512Combined sources
Beta strandi162 – 1643Combined sources
Beta strandi167 – 1715Combined sources
Helixi174 – 1818Combined sources
Helixi185 – 19713Combined sources
Beta strandi200 – 2045Combined sources
Beta strandi207 – 2115Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OZ6X-ray2.80A9-214[»]
ProteinModelPortaliP55222.
SMRiP55222. Positions 9-213.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55222.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini142 – 21473HTH crp-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000250565.
InParanoidiP55222.
KOiK10914.
OMAiRAKSACE.
OrthoDBiEOG69GZGV.
PhylomeDBiP55222.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55222-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVAITHTPKL KHLDKLLAHC HRRRYTAKST IIYAGDRCET LFFIIKGSVT
60 70 80 90 100
ILIEDDDGRE MIIGYLNSGD FFGELGLFEK EGSEQERSAW VRAKVECEVA
110 120 130 140 150
EISYAKFREL SQQDSEILYT LGSQMADRLR KTTRKVGDLA FLDVTGRVAR
160 170 180 190 200
TLLDLCQQPD AMTHPDGMQI KITRQEIGRI VGCSREMVGR VLKSLEEQGL
210
VHVKGKTMVV FGTR
Length:214
Mass (Da):24,225
Last modified:October 1, 1996 - v1
Checksum:i03B7F2A4C2CED7E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16318 Genomic DNA. Translation: AAA73464.1.
AE004091 Genomic DNA. Translation: AAG04041.1.
PIRiA55540.
RefSeqiNP_249343.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04041; AAG04041; PA0652.
GeneIDi880744.
KEGGipae:PA0652.
PATRICi19835566. VBIPseAer58763_0683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16318 Genomic DNA. Translation: AAA73464.1.
AE004091 Genomic DNA. Translation: AAG04041.1.
PIRiA55540.
RefSeqiNP_249343.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OZ6X-ray2.80A9-214[»]
ProteinModelPortaliP55222.
SMRiP55222. Positions 9-213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0652.

Protocols and materials databases

DNASUi880744.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04041; AAG04041; PA0652.
GeneIDi880744.
KEGGipae:PA0652.
PATRICi19835566. VBIPseAer58763_0683.

Organism-specific databases

PseudoCAPiPA0652.

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000250565.
InParanoidiP55222.
KOiK10914.
OMAiRAKSACE.
OrthoDBiEOG69GZGV.
PhylomeDBiP55222.

Miscellaneous databases

EvolutionaryTraceiP55222.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The vfr gene product, required for Pseudomonas aeruginosa exotoxin A and protease production, belongs to the cyclic AMP receptor protein family."
    West S.E.H., Sample A.K., Runyen-Janecky L.J.
    J. Bacteriol. 176:7532-7542(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiVFR_PSEAE
AccessioniPrimary (citable) accession number: P55222
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.