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P55217

- METB_ARATH

UniProt

P55217 - METB_ARATH

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Protein

Cystathionine gamma-synthase, chloroplastic

Gene

CGS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia (By similarity).By similarity

Catalytic activityi

O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

Cofactori

Pyridoxal phosphate.

Pathwayi

GO - Molecular functioni

  1. cystathionine gamma-synthase activity Source: TAIR
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. methionine biosynthetic process Source: TAIR
  2. selenium compound metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G01120-MONOMER.
MetaCyc:AT3G01120-MONOMER.
SABIO-RKP55217.
UniPathwayiUPA00051; UER00077.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-synthase, chloroplastic (EC:2.5.1.48)
Short name:
CGS
Alternative name(s):
O-succinylhomoserine (thiol)-lyase
Gene namesi
Name:CGS1
Synonyms:CYS1
Ordered Locus Names:At3g01120
ORF Names:T4P13.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G01120.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 182182ChloroplastSequence AnalysisAdd
BLAST
Chaini183 – 563381Cystathionine gamma-synthase, chloroplasticPRO_0000033455Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei379 – 3791N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiP55217.
PRIDEiP55217.

Expressioni

Gene expression databases

GenevestigatoriP55217.

Structurei

3D structure databases

ProteinModelPortaliP55217.
SMRiP55217. Positions 170-563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi13 – 186Poly-Ser
Compositional biasi104 – 1096Poly-Ala
Compositional biasi119 – 1257Poly-Ala
Compositional biasi128 – 1314Poly-Ala

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0626.
HOGENOMiHOG000246415.
InParanoidiP55217.
KOiK01739.
OMAiKFPLFTE.
PhylomeDBiP55217.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55217-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVSSFQCPT IFSSSSISGF QCRSDPDLVG SPVGGSSRRR VHASAGISSS
60 70 80 90 100
FTGDAGLSSR ILRFPPNFVR QLSIKARRNC SNIGVAQIVA AKWSNNPSSA
110 120 130 140 150
LPSAAAAAAT SSASAVSSAA SAAAASSAAA APVAAAPPVV LKSVDEEVVV
160 170 180 190 200
AEEGIREKIG SVQLTDSKHS FLSSDGSLTV HAGERLGRGI VTDAITTPVV
210 220 230 240 250
NTSAYFFKKT AELIDFKEKR SVSFEYGRYG NPTTVVLEDK ISALEGAEST
260 270 280 290 300
LVMASGMCAS TVMLLALVPA GGHIVTTTDC YRKTRIFMEN FLPKLGITVT
310 320 330 340 350
VIDPADIAGL EAAVNEFKVS LFFTESPTNP FLRCVDIELV SKICHKRGTL
360 370 380 390 400
VCIDGTFATP LNQKALALGA DLVVHSATKY IGGHNDVLAG CICGSLKLVS
410 420 430 440 450
EIRNLHHVLG GTLNPNAAYL IIRGMKTLHL RVQQQNSTAF RMAEILEAHP
460 470 480 490 500
KVSHVYYPGL PSHPEHELAK RQMTGFGGVV SFEIDGDIET TIKFVDSLKI
510 520 530 540 550
PYIAPSFGGC ESIVDQPAIM SYWDLPQEER LKYGIKDNLV RFSFGVEDFE
560
DVKADILQAL EAI
Length:563
Mass (Da):59,919
Last modified:June 20, 2002 - v3
Checksum:i4FB902896C062860
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021P → T in CAA64383. 1 PublicationCurated
Sequence conflicti177 – 1771S → Q in CAA64383. 1 PublicationCurated
Sequence conflicti547 – 5471E → K in AAM13883. (PubMed:14593172)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti8 – 81C → S.2 Publications
Natural varianti55 – 551A → G.2 Publications
Natural varianti91 – 911A → G.2 Publications
Natural varianti412 – 4121T → P.2 Publications
Natural varianti459 – 4591G → A.1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94756 mRNA. Translation: CAA64383.1.
U43709 mRNA. Translation: AAC49574.1.
U83500 mRNA. Translation: AAB41235.1.
AB010888 Genomic DNA. Translation: BAA24699.1.
AF039206 Genomic DNA. Translation: AAC25687.1.
AC008261 Genomic DNA. Translation: AAF26162.1.
CP002686 Genomic DNA. Translation: AEE73612.1.
AY094438 mRNA. Translation: AAM19810.1.
AY091062 mRNA. Translation: AAM13883.1.
BT002753 mRNA. Translation: AAO22582.1.
X79707 Genomic DNA. Translation: CAA56143.1.
PIRiS51579.
S71228.
RefSeqiNP_186761.1. NM_110977.2.
UniGeneiAt.20198.

Genome annotation databases

EnsemblPlantsiAT3G01120.1; AT3G01120.1; AT3G01120.
GeneIDi821292.
KEGGiath:AT3G01120.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94756 mRNA. Translation: CAA64383.1 .
U43709 mRNA. Translation: AAC49574.1 .
U83500 mRNA. Translation: AAB41235.1 .
AB010888 Genomic DNA. Translation: BAA24699.1 .
AF039206 Genomic DNA. Translation: AAC25687.1 .
AC008261 Genomic DNA. Translation: AAF26162.1 .
CP002686 Genomic DNA. Translation: AEE73612.1 .
AY094438 mRNA. Translation: AAM19810.1 .
AY091062 mRNA. Translation: AAM13883.1 .
BT002753 mRNA. Translation: AAO22582.1 .
X79707 Genomic DNA. Translation: CAA56143.1 .
PIRi S51579.
S71228.
RefSeqi NP_186761.1. NM_110977.2.
UniGenei At.20198.

3D structure databases

ProteinModelPortali P55217.
SMRi P55217. Positions 170-563.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P55217.
PRIDEi P55217.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G01120.1 ; AT3G01120.1 ; AT3G01120 .
GeneIDi 821292.
KEGGi ath:AT3G01120.

Organism-specific databases

TAIRi AT3G01120.

Phylogenomic databases

eggNOGi COG0626.
HOGENOMi HOG000246415.
InParanoidi P55217.
KOi K01739.
OMAi KFPLFTE.
PhylomeDBi P55217.

Enzyme and pathway databases

UniPathwayi UPA00051 ; UER00077 .
BioCyci ARA:AT3G01120-MONOMER.
MetaCyc:AT3G01120-MONOMER.
SABIO-RK P55217.

Gene expression databases

Genevestigatori P55217.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
PANTHERi PTHR11808. PTHR11808. 1 hit.
Pfami PF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Lessard P., Kreis M., Thomas M.
    Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Cloning and analysis of the gene for cystathionine gamma-synthase from Arabidopsis thaliana."
    Kim J., Leustek T.
    Plant Mol. Biol. 32:1117-1124(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS SER-8; GLY-55; GLY-91 AND ALA-459.
    Strain: cv. Landsberg erecta.
  3. Gakiere B., Ravanel S., Job D., Douce R.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: cv. Columbia.
  4. "Nucleotide sequence polymorphisms in the cystathionine gamma-synthase gene of Arabidopsis thaliana."
    Kim J., Chiba Y., Yamamoto A., Naito S., Leustek T.
    Plant Gene Register PGR99-087
    Cited for: NUCLEOTIDE SEQUENCE, VARIANTS SER-8; GLY-55; GLY-91 AND PRO-412.
    Strain: cv. Columbia and cv. Landsberg erecta.
  5. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  6. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  8. "Gene density and organization in a small region of the Arabidopsis thaliana genome."
    le Guen L., Thomas M., Kreis M.
    Mol. Gen. Genet. 245:390-396(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 340-563, VARIANT PRO-412.
    Strain: cv. Columbia.

Entry informationi

Entry nameiMETB_ARATH
AccessioniPrimary (citable) accession number: P55217
Secondary accession number(s): P92944, P93038, Q42550
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 20, 2002
Last modified: October 1, 2014
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3