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Protein

Cystathionine gamma-synthase 1, chloroplastic

Gene

CGS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first committed step of methionine (Met) biosynthesis. Catalyzes the formation of L-cystathionine from homoserine esters and L-cysteine, via a gamma-replacement reaction. Substrate preference for cystathionine synthesis is O-phospho-L-homoserine (OPH) > O(4)-succinyl-L-homoserine (OSH) >> O-acetyl-L-homoserine (OAH). Is able, at extremely low rate, to catalyze a gamma-elimination of OPH in the absence of cysteine to produce inorganic phosphate (Pi), 2-oxobutanoate and ammonia.1 Publication

Catalytic activityi

O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.1 Publication

Cofactori

pyridoxal 5'-phosphate1 PublicationNote: Binds 1 pyridoxal 5'-phosphate per subunit.1 Publication

Enzyme regulationi

Inhibited by propargylglycine.1 Publication

Kineticsi

kcat is 30 sec(-1) with L-cysteine as substrate.1 Publication

Manual assertion based on experiment ini

  1. KM=0.46 mM for L-cysteine1 Publication
  2. KM=2.5 mM for O-phospho-L-homoserine1 Publication
  1. Vmax=33.6 µmol/min/mg enzyme with L-cysteine as substrate1 Publication

pH dependencei

Optimum pH is 7.5.1 Publication

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine.Curated
Proteins known to be involved in this subpathway in this organism are:
  1. Probable cystathionine gamma-synthase 2 (CGS2), Cystathionine gamma-synthase 1, chloroplastic (CGS1)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • cystathionine gamma-synthase activity Source: TAIR
  • pyridoxal phosphate binding Source: UniProtKB

GO - Biological processi

  • 'de novo' L-methionine biosynthetic process Source: GO_Central
  • cysteine biosynthetic process via cystathionine Source: GO_Central
  • methionine biosynthetic process Source: TAIR
  • selenium compound metabolic process Source: TAIR
  • transsulfuration Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G01120-MONOMER.
MetaCyc:AT3G01120-MONOMER.
BRENDAi2.5.1.48. 399.
SABIO-RKP55217.
UniPathwayiUPA00051; UER00077.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-synthase 1, chloroplasticCurated (EC:2.5.1.481 Publication)
Short name:
AtCGS1Curated
Alternative name(s):
METHIONINE OVERACCUMULATION 11 Publication
O-succinylhomoserine (thiol)-lyase
Gene namesi
Name:CGS1Curated
Synonyms:CYS1Curated, MTO11 Publication
Ordered Locus Names:At3g01120
ORF Names:T4P13.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G01120.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi77R → H in mto1-4; over-accumulation of soluble methionine. 1 Publication1
Mutagenesisi78R → K in mto1-7; over-accumulation of soluble methionine. 1 Publication1
Mutagenesisi81S → N in mto1-2; over-accumulation of soluble methionine. 1 Publication1
Mutagenesisi84G → D in mto1-3 and mto1-5; over-accumulation of soluble methionine. 1 Publication1
Mutagenesisi84G → S in mto1-1; over-accumulation of soluble methionine. 1 Publication1
Mutagenesisi86A → V in mto1-6; over-accumulation of soluble methionine. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 68Chloroplast1 PublicationAdd BLAST68
ChainiPRO_000003345569 – 563Cystathionine gamma-synthase 1, chloroplasticAdd BLAST495

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei379N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP55217.
PRIDEiP55217.

Expressioni

Inductioni

Down-regulated by methionine.1 Publication

Gene expression databases

GenevisibleiP55217. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G01120.1.

Structurei

3D structure databases

ProteinModelPortaliP55217.
SMRiP55217.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi104 – 136Ala-richSequence analysisAdd BLAST33

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0053. Eukaryota.
COG0626. LUCA.
HOGENOMiHOG000246415.
InParanoidiP55217.
KOiK01739.
OMAiFLAIVNC.
OrthoDBiEOG093609C5.
PhylomeDBiP55217.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55217-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSSFQCPT IFSSSSISGF QCRSDPDLVG SPVGGSSRRR VHASAGISSS
60 70 80 90 100
FTGDAGLSSR ILRFPPNFVR QLSIKARRNC SNIGVAQIVA AKWSNNPSSA
110 120 130 140 150
LPSAAAAAAT SSASAVSSAA SAAAASSAAA APVAAAPPVV LKSVDEEVVV
160 170 180 190 200
AEEGIREKIG SVQLTDSKHS FLSSDGSLTV HAGERLGRGI VTDAITTPVV
210 220 230 240 250
NTSAYFFKKT AELIDFKEKR SVSFEYGRYG NPTTVVLEDK ISALEGAEST
260 270 280 290 300
LVMASGMCAS TVMLLALVPA GGHIVTTTDC YRKTRIFMEN FLPKLGITVT
310 320 330 340 350
VIDPADIAGL EAAVNEFKVS LFFTESPTNP FLRCVDIELV SKICHKRGTL
360 370 380 390 400
VCIDGTFATP LNQKALALGA DLVVHSATKY IGGHNDVLAG CICGSLKLVS
410 420 430 440 450
EIRNLHHVLG GTLNPNAAYL IIRGMKTLHL RVQQQNSTAF RMAEILEAHP
460 470 480 490 500
KVSHVYYPGL PSHPEHELAK RQMTGFGGVV SFEIDGDIET TIKFVDSLKI
510 520 530 540 550
PYIAPSFGGC ESIVDQPAIM SYWDLPQEER LKYGIKDNLV RFSFGVEDFE
560
DVKADILQAL EAI
Length:563
Mass (Da):59,919
Last modified:June 20, 2002 - v3
Checksum:i4FB902896C062860
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102P → T in CAA64383 (Ref. 4) Curated1
Sequence conflicti177S → Q in CAA64383 (Ref. 4) Curated1
Sequence conflicti547E → K in AAM13883 (PubMed:14593172).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti8C → S.3 Publications1
Natural varianti55A → G.3 Publications1
Natural varianti91A → G.3 Publications1
Natural varianti412T → P.3 Publications1
Natural varianti459G → A.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43709 mRNA. Translation: AAC49574.1.
U83500 mRNA. Translation: AAB41235.1.
AB010888 Genomic DNA. Translation: BAA24699.1.
AF039206 Genomic DNA. Translation: AAC25687.1.
X94756 mRNA. Translation: CAA64383.1.
AC008261 Genomic DNA. Translation: AAF26162.1.
CP002686 Genomic DNA. Translation: AEE73612.1.
AY094438 mRNA. Translation: AAM19810.1.
AY091062 mRNA. Translation: AAM13883.1.
BT002753 mRNA. Translation: AAO22582.1.
X79707 Genomic DNA. Translation: CAA56143.1.
PIRiS51579.
S71228.
RefSeqiNP_186761.1. NM_110977.3.
UniGeneiAt.20198.

Genome annotation databases

EnsemblPlantsiAT3G01120.1; AT3G01120.1; AT3G01120.
GeneIDi821292.
GrameneiAT3G01120.1; AT3G01120.1; AT3G01120.
KEGGiath:AT3G01120.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43709 mRNA. Translation: AAC49574.1.
U83500 mRNA. Translation: AAB41235.1.
AB010888 Genomic DNA. Translation: BAA24699.1.
AF039206 Genomic DNA. Translation: AAC25687.1.
X94756 mRNA. Translation: CAA64383.1.
AC008261 Genomic DNA. Translation: AAF26162.1.
CP002686 Genomic DNA. Translation: AEE73612.1.
AY094438 mRNA. Translation: AAM19810.1.
AY091062 mRNA. Translation: AAM13883.1.
BT002753 mRNA. Translation: AAO22582.1.
X79707 Genomic DNA. Translation: CAA56143.1.
PIRiS51579.
S71228.
RefSeqiNP_186761.1. NM_110977.3.
UniGeneiAt.20198.

3D structure databases

ProteinModelPortaliP55217.
SMRiP55217.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G01120.1.

Proteomic databases

PaxDbiP55217.
PRIDEiP55217.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G01120.1; AT3G01120.1; AT3G01120.
GeneIDi821292.
GrameneiAT3G01120.1; AT3G01120.1; AT3G01120.
KEGGiath:AT3G01120.

Organism-specific databases

TAIRiAT3G01120.

Phylogenomic databases

eggNOGiKOG0053. Eukaryota.
COG0626. LUCA.
HOGENOMiHOG000246415.
InParanoidiP55217.
KOiK01739.
OMAiFLAIVNC.
OrthoDBiEOG093609C5.
PhylomeDBiP55217.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00077.
BioCyciARA:AT3G01120-MONOMER.
MetaCyc:AT3G01120-MONOMER.
BRENDAi2.5.1.48. 399.
SABIO-RKP55217.

Miscellaneous databases

PROiP55217.

Gene expression databases

GenevisibleiP55217. AT.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCGS1_ARATH
AccessioniPrimary (citable) accession number: P55217
Secondary accession number(s): P92944, P93038, Q42550
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 20, 2002
Last modified: November 30, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

A DNA region of the first exon coding for a conserved motif of 11 amino acids in CGS1 (positions 77-87) is required for post-transcriptional autoregulation and acts in cis to down-regulate its own mRNA stability in response to excess methionine. This conserved motif is dispensable for CGS enzymatic activity and only found in plant CGSs (PubMed:10558994, PubMed:12121993). It is unclear whether the transit peptide cleavage site is between Phe-68 and Val-69 (PubMed:9531508) or Ala-90 and Ala-91 (PubMed:25146485).4 Publications

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.