P55217 (METB_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cystathionine gamma-synthase, chloroplastic
Short name=CGS
EC=2.5.1.48

Alternative name(s):
O-succinylhomoserine (thiol)-lyase

Gene names

Name:	CGS1
Synonyms:	CYS1
Ordered Locus Names:	At3g01120
ORF Names:	T4P13.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia By similarity.
Catalytic activity	O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Polymorphism
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionine biosynthetic process Inferred from mutant phenotype PubMed 12232133. Source: TAIR selenium compound metabolic process Inferred from direct assay PubMed 14618405. Source: TAIR
Cellular_component	chloroplast stroma Inferred from direct assay PubMed 20061580. Source: TAIR
Molecular_function	cystathionine gamma-synthase activity Inferred from direct assay PubMed 9531508. Source: TAIR pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 182	182	Chloroplast Potential
	Chain	183 – 563	381	Cystathionine gamma-synthase, chloroplastic		PRO_0000033455
Regions
	Compositional bias	13 – 18	6	Poly-Ser
	Compositional bias	104 – 109	6	Poly-Ala
	Compositional bias	119 – 125	7	Poly-Ala
	Compositional bias	128 – 131	4	Poly-Ala
Amino acid modifications
	Modified residue	379	1	N6-(pyridoxal phosphate)lysine By similarity
Natural variations
	Natural variant	8	1	C → S. Ref.2 Ref.4
	Natural variant	55	1	A → G. Ref.2 Ref.4
	Natural variant	91	1	A → G. Ref.2 Ref.4
	Natural variant	412	1	T → P. Ref.4 Ref.8
	Natural variant	459	1	G → A. Ref.2
Experimental info
	Sequence conflict	102	1	P → T in CAA64383. Ref.1
	Sequence conflict	177	1	S → Q in CAA64383. Ref.1
	Sequence conflict	547	1	E → K in AAM13883. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P55217 [UniParc]. Last modified June 20, 2002. Version 3. Checksum: 4FB902896C062860	FASTA	563	59,919
	10 20 30 40 50 60 MAVSSFQCPT IFSSSSISGF QCRSDPDLVG SPVGGSSRRR VHASAGISSS FTGDAGLSSR 70 80 90 100 110 120 ILRFPPNFVR QLSIKARRNC SNIGVAQIVA AKWSNNPSSA LPSAAAAAAT SSASAVSSAA 130 140 150 160 170 180 SAAAASSAAA APVAAAPPVV LKSVDEEVVV AEEGIREKIG SVQLTDSKHS FLSSDGSLTV 190 200 210 220 230 240 HAGERLGRGI VTDAITTPVV NTSAYFFKKT AELIDFKEKR SVSFEYGRYG NPTTVVLEDK 250 260 270 280 290 300 ISALEGAEST LVMASGMCAS TVMLLALVPA GGHIVTTTDC YRKTRIFMEN FLPKLGITVT 310 320 330 340 350 360 VIDPADIAGL EAAVNEFKVS LFFTESPTNP FLRCVDIELV SKICHKRGTL VCIDGTFATP 370 380 390 400 410 420 LNQKALALGA DLVVHSATKY IGGHNDVLAG CICGSLKLVS EIRNLHHVLG GTLNPNAAYL 430 440 450 460 470 480 IIRGMKTLHL RVQQQNSTAF RMAEILEAHP KVSHVYYPGL PSHPEHELAK RQMTGFGGVV 490 500 510 520 530 540 SFEIDGDIET TIKFVDSLKI PYIAPSFGGC ESIVDQPAIM SYWDLPQEER LKYGIKDNLV 550 560 RFSFGVEDFE DVKADILQAL EAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Lessard P., Kreis M., Thomas M. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Cloning and analysis of the gene for cystathionine gamma-synthase from Arabidopsis thaliana." Kim J., Leustek T. Plant Mol. Biol. 32:1117-1124(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS SER-8; GLY-55; GLY-91 AND ALA-459. Strain: cv. Landsberg erecta.
[3]	Gakiere B., Ravanel S., Job D., Douce R. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Columbia.
[4]	"Nucleotide sequence polymorphisms in the cystathionine gamma-synthase gene of Arabidopsis thaliana." Kim J., Chiba Y., Yamamoto A., Naito S., Leustek T. Plant Gene Register PGR99-087 Cited for: NUCLEOTIDE SEQUENCE, VARIANTS SER-8; GLY-55; GLY-91 AND PRO-412. Strain: cv. Columbia and cv. Landsberg erecta.
[5]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[6]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[7]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[8]	"Gene density and organization in a small region of the Arabidopsis thaliana genome." le Guen L., Thomas M., Kreis M. Mol. Gen. Genet. 245:390-396(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 340-563, VARIANT PRO-412. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94756 mRNA. Translation: CAA64383.1. U43709 mRNA. Translation: AAC49574.1. U83500 mRNA. Translation: AAB41235.1. AB010888 Genomic DNA. Translation: BAA24699.1. AF039206 Genomic DNA. Translation: AAC25687.1. AC008261 Genomic DNA. Translation: AAF26162.1. CP002686 Genomic DNA. Translation: AEE73612.1. AY094438 mRNA. Translation: AAM19810.1. AY091062 mRNA. Translation: AAM13883.1. BT002753 mRNA. Translation: AAO22582.1. X79707 Genomic DNA. Translation: CAA56143.1.
IPI	IPI00534631.
PIR	S51579. S71228.
RefSeq	NP_186761.1. NM_110977.2.
UniGene	At.20198.
3D structure databases
ProteinModelPortal	P55217.
SMR	P55217. Positions 170-563.
ModBase	Search...
Proteomic databases
PaxDb	P55217.
PRIDE	P55217.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G01120.1; AT3G01120.1; AT3G01120.
GeneID	821292.
KEGG	ath:AT3G01120.
Organism-specific databases
TAIR	At3g01120.
Phylogenomic databases
eggNOG	COG0626.
HOGENOM	HOG000246415.
InParanoid	P55217.
KO	K01739.
OMA	NAEHGAN.
PhylomeDB	P55217.
ProtClustDB	CLSN2679254.
Enzyme and pathway databases
BioCyc	ARA:AT3G01120-MONOMER. MetaCyc:AT3G01120-MONOMER.
UniPathway	UPA00051; UER00077.
Gene expression databases
Genevestigator	P55217.
GermOnline	AT3G01120. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11808. PTHR11808. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METB_ARATH

Accession

Primary (citable) accession number: P55217
Secondary accession number(s): P92944, P93038, Q42550

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 20, 2002
Last modified:	May 1, 2013
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program