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P55217

- METB_ARATH

UniProt

P55217 - METB_ARATH

Protein

Cystathionine gamma-synthase, chloroplastic

Gene

CGS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 3 (20 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia By similarity.By similarity

    Catalytic activityi

    O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    GO - Molecular functioni

    1. cystathionine gamma-synthase activity Source: TAIR
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. methionine biosynthetic process Source: TAIR
    2. selenium compound metabolic process Source: TAIR

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciARA:AT3G01120-MONOMER.
    MetaCyc:AT3G01120-MONOMER.
    SABIO-RKP55217.
    UniPathwayiUPA00051; UER00077.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cystathionine gamma-synthase, chloroplastic (EC:2.5.1.48)
    Short name:
    CGS
    Alternative name(s):
    O-succinylhomoserine (thiol)-lyase
    Gene namesi
    Name:CGS1
    Synonyms:CYS1
    Ordered Locus Names:At3g01120
    ORF Names:T4P13.19
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G01120.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 182182ChloroplastSequence AnalysisAdd
    BLAST
    Chaini183 – 563381Cystathionine gamma-synthase, chloroplasticPRO_0000033455Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei379 – 3791N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    PaxDbiP55217.
    PRIDEiP55217.

    Expressioni

    Gene expression databases

    GenevestigatoriP55217.

    Structurei

    3D structure databases

    ProteinModelPortaliP55217.
    SMRiP55217. Positions 170-563.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi13 – 186Poly-Ser
    Compositional biasi104 – 1096Poly-Ala
    Compositional biasi119 – 1257Poly-Ala
    Compositional biasi128 – 1314Poly-Ala

    Sequence similaritiesi

    Belongs to the trans-sulfuration enzymes family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0626.
    HOGENOMiHOG000246415.
    InParanoidiP55217.
    KOiK01739.
    OMAiKFPLFTE.
    PhylomeDBiP55217.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PANTHERiPTHR11808. PTHR11808. 1 hit.
    PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P55217-1 [UniParc]FASTAAdd to Basket

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    MAVSSFQCPT IFSSSSISGF QCRSDPDLVG SPVGGSSRRR VHASAGISSS    50
    FTGDAGLSSR ILRFPPNFVR QLSIKARRNC SNIGVAQIVA AKWSNNPSSA 100
    LPSAAAAAAT SSASAVSSAA SAAAASSAAA APVAAAPPVV LKSVDEEVVV 150
    AEEGIREKIG SVQLTDSKHS FLSSDGSLTV HAGERLGRGI VTDAITTPVV 200
    NTSAYFFKKT AELIDFKEKR SVSFEYGRYG NPTTVVLEDK ISALEGAEST 250
    LVMASGMCAS TVMLLALVPA GGHIVTTTDC YRKTRIFMEN FLPKLGITVT 300
    VIDPADIAGL EAAVNEFKVS LFFTESPTNP FLRCVDIELV SKICHKRGTL 350
    VCIDGTFATP LNQKALALGA DLVVHSATKY IGGHNDVLAG CICGSLKLVS 400
    EIRNLHHVLG GTLNPNAAYL IIRGMKTLHL RVQQQNSTAF RMAEILEAHP 450
    KVSHVYYPGL PSHPEHELAK RQMTGFGGVV SFEIDGDIET TIKFVDSLKI 500
    PYIAPSFGGC ESIVDQPAIM SYWDLPQEER LKYGIKDNLV RFSFGVEDFE 550
    DVKADILQAL EAI 563
    Length:563
    Mass (Da):59,919
    Last modified:June 20, 2002 - v3
    Checksum:i4FB902896C062860
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti102 – 1021P → T in CAA64383. 1 PublicationCurated
    Sequence conflicti177 – 1771S → Q in CAA64383. 1 PublicationCurated
    Sequence conflicti547 – 5471E → K in AAM13883. (PubMed:14593172)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti8 – 81C → S.2 Publications
    Natural varianti55 – 551A → G.2 Publications
    Natural varianti91 – 911A → G.2 Publications
    Natural varianti412 – 4121T → P.2 Publications
    Natural varianti459 – 4591G → A.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94756 mRNA. Translation: CAA64383.1.
    U43709 mRNA. Translation: AAC49574.1.
    U83500 mRNA. Translation: AAB41235.1.
    AB010888 Genomic DNA. Translation: BAA24699.1.
    AF039206 Genomic DNA. Translation: AAC25687.1.
    AC008261 Genomic DNA. Translation: AAF26162.1.
    CP002686 Genomic DNA. Translation: AEE73612.1.
    AY094438 mRNA. Translation: AAM19810.1.
    AY091062 mRNA. Translation: AAM13883.1.
    BT002753 mRNA. Translation: AAO22582.1.
    X79707 Genomic DNA. Translation: CAA56143.1.
    PIRiS51579.
    S71228.
    RefSeqiNP_186761.1. NM_110977.2.
    UniGeneiAt.20198.

    Genome annotation databases

    EnsemblPlantsiAT3G01120.1; AT3G01120.1; AT3G01120.
    GeneIDi821292.
    KEGGiath:AT3G01120.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94756 mRNA. Translation: CAA64383.1 .
    U43709 mRNA. Translation: AAC49574.1 .
    U83500 mRNA. Translation: AAB41235.1 .
    AB010888 Genomic DNA. Translation: BAA24699.1 .
    AF039206 Genomic DNA. Translation: AAC25687.1 .
    AC008261 Genomic DNA. Translation: AAF26162.1 .
    CP002686 Genomic DNA. Translation: AEE73612.1 .
    AY094438 mRNA. Translation: AAM19810.1 .
    AY091062 mRNA. Translation: AAM13883.1 .
    BT002753 mRNA. Translation: AAO22582.1 .
    X79707 Genomic DNA. Translation: CAA56143.1 .
    PIRi S51579.
    S71228.
    RefSeqi NP_186761.1. NM_110977.2.
    UniGenei At.20198.

    3D structure databases

    ProteinModelPortali P55217.
    SMRi P55217. Positions 170-563.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P55217.
    PRIDEi P55217.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G01120.1 ; AT3G01120.1 ; AT3G01120 .
    GeneIDi 821292.
    KEGGi ath:AT3G01120.

    Organism-specific databases

    TAIRi AT3G01120.

    Phylogenomic databases

    eggNOGi COG0626.
    HOGENOMi HOG000246415.
    InParanoidi P55217.
    KOi K01739.
    OMAi KFPLFTE.
    PhylomeDBi P55217.

    Enzyme and pathway databases

    UniPathwayi UPA00051 ; UER00077 .
    BioCyci ARA:AT3G01120-MONOMER.
    MetaCyc:AT3G01120-MONOMER.
    SABIO-RK P55217.

    Gene expression databases

    Genevestigatori P55217.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    PANTHERi PTHR11808. PTHR11808. 1 hit.
    Pfami PF01053. Cys_Met_Meta_PP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00868. CYS_MET_METAB_PP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Lessard P., Kreis M., Thomas M.
      Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Cloning and analysis of the gene for cystathionine gamma-synthase from Arabidopsis thaliana."
      Kim J., Leustek T.
      Plant Mol. Biol. 32:1117-1124(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS SER-8; GLY-55; GLY-91 AND ALA-459.
      Strain: cv. Landsberg erecta.
    3. Gakiere B., Ravanel S., Job D., Douce R.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: cv. Columbia.
    4. "Nucleotide sequence polymorphisms in the cystathionine gamma-synthase gene of Arabidopsis thaliana."
      Kim J., Chiba Y., Yamamoto A., Naito S., Leustek T.
      Plant Gene Register PGR99-087
      Cited for: NUCLEOTIDE SEQUENCE, VARIANTS SER-8; GLY-55; GLY-91 AND PRO-412.
      Strain: cv. Columbia and cv. Landsberg erecta.
    5. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    6. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    8. "Gene density and organization in a small region of the Arabidopsis thaliana genome."
      le Guen L., Thomas M., Kreis M.
      Mol. Gen. Genet. 245:390-396(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 340-563, VARIANT PRO-412.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiMETB_ARATH
    AccessioniPrimary (citable) accession number: P55217
    Secondary accession number(s): P92944, P93038, Q42550
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: June 20, 2002
    Last modified: October 1, 2014
    This is version 116 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3