Reviewed, UniProtKB/Swiss-Prot P55217 (METB_ARATH)

Names and origin

Protein names

Recommended name:
    Cystathionine gamma-synthase, chloroplastic
      Short name=CGS
    EC=2.5.1.48
Alternative name(s):
    O-succinylhomoserine (thiol)-lyase

Gene names

Name:	CGS1
Synonyms:	CYS1
Ordered Locus Names:	At3g01120
ORF Names:	T4P13.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Polymorphism
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from mutant phenotype. Source: TAIR
Cellular component	chloroplast Inferred from direct assay. Source: TAIR
Molecular function	cystathionine gamma-synthase activity Inferred from direct assay. Source: TAIR pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 182	182	Chloroplast Potential
	Chain	183 – 563	381	Cystathionine gamma-synthase, chloroplastic		PRO_0000033455
Regions
	Compositional bias	13 – 18	6	Poly-Ser
	Compositional bias	104 – 109	6	Poly-Ala
	Compositional bias	119 – 125	7	Poly-Ala
	Compositional bias	128 – 131	4	Poly-Ala
Amino acid modifications
	Modified residue	379	1	N6-(pyridoxal phosphate)lysine By similarity
Natural variations
	Natural variant	8	1	C → S
	Natural variant	55	1	A → G
	Natural variant	91	1	A → G
	Natural variant	412	1	T → P
	Natural variant	459	1	G → A
Experimental info
	Sequence conflict	102	1	P → T in CAA64383. Ref.1
	Sequence conflict	177	1	S → Q in CAA64383. Ref.1
	Sequence conflict	547	1	E → K in AAM13883. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P55217-1 [UniParc]. Last modified June 20, 2002. Version 3. Checksum: 4FB902896C062860	FASTA	563	59,919
	10 20 30 40 50 60 MAVSSFQCPT IFSSSSISGF QCRSDPDLVG SPVGGSSRRR VHASAGISSS FTGDAGLSSR 70 80 90 100 110 120 ILRFPPNFVR QLSIKARRNC SNIGVAQIVA AKWSNNPSSA LPSAAAAAAT SSASAVSSAA 130 140 150 160 170 180 SAAAASSAAA APVAAAPPVV LKSVDEEVVV AEEGIREKIG SVQLTDSKHS FLSSDGSLTV 190 200 210 220 230 240 HAGERLGRGI VTDAITTPVV NTSAYFFKKT AELIDFKEKR SVSFEYGRYG NPTTVVLEDK 250 260 270 280 290 300 ISALEGAEST LVMASGMCAS TVMLLALVPA GGHIVTTTDC YRKTRIFMEN FLPKLGITVT 310 320 330 340 350 360 VIDPADIAGL EAAVNEFKVS LFFTESPTNP FLRCVDIELV SKICHKRGTL VCIDGTFATP 370 380 390 400 410 420 LNQKALALGA DLVVHSATKY IGGHNDVLAG CICGSLKLVS EIRNLHHVLG GTLNPNAAYL 430 440 450 460 470 480 IIRGMKTLHL RVQQQNSTAF RMAEILEAHP KVSHVYYPGL PSHPEHELAK RQMTGFGGVV 490 500 510 520 530 540 SFEIDGDIET TIKFVDSLKI PYIAPSFGGC ESIVDQPAIM SYWDLPQEER LKYGIKDNLV 550 560 RFSFGVEDFE DVKADILQAL EAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Lessard P., Kreis M., Thomas M. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Cloning and analysis of the gene for cystathionine gamma-synthase from Arabidopsis thaliana." Kim J., Leustek T. Plant Mol. Biol. 32:1117-1124(1996) [PubMed: 9002610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS SER-8; GLY-55; GLY-91 AND ALA-459. Strain: cv. Landsberg erecta.
[3]	Gakiere B., Ravanel S., Job D., Douce R. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Columbia.
[4]	"Nucleotide sequence polymorphisms in the cystathionine gamma-synthase gene of Arabidopsis thaliana." Kim J., Chiba Y., Yamamoto A., Naito S., Leustek T. Plant Gene Register PGR99-087 Cited for: NUCLEOTIDE SEQUENCE, VARIANTS SER-8; GLY-55; GLY-91 AND PRO-412. Strain: cv. Columbia and cv. Landsberg erecta.
[5]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Gene density and organization in a small region of the Arabidopsis thaliana genome." le Guen L., Thomas M., Kreis M. Mol. Gen. Genet. 245:390-396(1994) [PubMed: 7816049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 340-563, VARIANT PRO-412. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X94756 mRNA. Translation: CAA64383.1. U43709 mRNA. Translation: AAC49574.1. U83500 mRNA. Translation: AAB41235.1. AB010888 Genomic DNA. Translation: BAA24699.1. AF039206 Genomic DNA. Translation: AAC25687.1. AC008261 Genomic DNA. Translation: AAF26162.1. AY094438 mRNA. Translation: AAM19810.1. AY091062 mRNA. Translation: AAM13883.1. BT002753 mRNA. Translation: AAO22582.1. X79707 Genomic DNA. Translation: CAA56143.1.
IPI	IPI00534631.
PIR	S51579. S71228.
RefSeq	NP_186761.1.
UniGene	At.20198
3D structure databases
HSSP	HSSP built from PDB template 1QGN based on UniProtKB Q9ZPL5.
SMR	P55217. Positions 170-563.
ModBase	Search...
Protein-protein interaction databases
STRING	P55217.
Proteomic databases
PRIDE	P55217.
Genome annotation databases
GeneID	821292.
GenomeReviews	Gene locus AT3G01120 in contig BA000014_GR.
KEGG	ath:AT3G01120.
NMPDR	fig|3702.1.peg.12026.
Organism-specific databases
TAIR	At3g01120.
Phylogenomic databases
OMA	VDRISEN.
Enzyme and pathway databases
BRENDA	2.5.1.48. 302.
Gene expression databases
ArrayExpress	P55217.
Genevestigator	P55217.
GermOnline	AT3G01120. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METB_ARATH

Accession

Primary (citable) accession number: P55217
Secondary accession number(s): P92944, P93038, Q42550

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 20, 2002
Last modified:	November 3, 2009
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)