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Protein

Cystathionine gamma-synthase 1, chloroplastic

Gene

CGS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first committed step of methionine (Met) biosynthesis. Catalyzes the formation of L-cystathionine from homoserine esters and L-cysteine, via a gamma-replacement reaction. Substrate preference for cystathionine synthesis is O-phospho-L-homoserine (OPH) > O4-succinyl-L-homoserine (OSH) >> O-acetyl-L-homoserine (OAH). Is able, at extremely low rate, to catalyze a gamma-elimination of OPH in the absence of cysteine to produce inorganic phosphate (Pi), 2-oxobutanoate and ammonia.1 Publication

Miscellaneous

A DNA region of the first exon coding for a conserved motif of 11 amino acids in CGS1 (positions 77-87) is required for post-transcriptional autoregulation and acts in cis to down-regulate its own mRNA stability in response to excess methionine. This conserved motif is dispensable for CGS enzymatic activity and only found in plant CGSs (PubMed:10558994, PubMed:12121993). It is unclear whether the transit peptide cleavage site is between Phe-68 and Val-69 (PubMed:9531508) or Ala-90 and Ala-91 (PubMed:25146485).4 Publications

Catalytic activityi

O4-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.1 Publication

Cofactori

pyridoxal 5'-phosphate1 PublicationNote: Binds 1 pyridoxal 5'-phosphate per subunit.1 Publication

Enzyme regulationi

Inhibited by propargylglycine.1 Publication

Kineticsi

kcat is 30 sec(-1) with L-cysteine as substrate.1 Publication
  1. KM=0.46 mM for L-cysteine1 Publication
  2. KM=2.5 mM for O-phospho-L-homoserine1 Publication
  1. Vmax=33.6 µmol/min/mg enzyme with L-cysteine as substrate1 Publication

pH dependencei

Optimum pH is 7.5.1 Publication

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine.Curated
Proteins known to be involved in this subpathway in this organism are:
  1. Probable cystathionine gamma-synthase 2 (CGS2), Cystathionine gamma-synthase 1, chloroplastic (CGS1)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • cystathionine gamma-synthase activity Source: TAIR
  • pyridoxal phosphate binding Source: UniProtKB

GO - Biological processi

  • methionine biosynthetic process Source: TAIR
  • selenium compound metabolic process Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G01120-MONOMER
MetaCyc:AT3G01120-MONOMER
BRENDAi2.5.1.48 399
ReactomeiR-ATH-1614558 Degradation of cysteine and homocysteine
R-ATH-1614603 Cysteine formation from homocysteine
SABIO-RKiP55217
UniPathwayiUPA00051; UER00077

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-synthase 1, chloroplasticCurated (EC:2.5.1.481 Publication)
Short name:
AtCGS1Curated
Alternative name(s):
METHIONINE OVERACCUMULATION 11 Publication
O-succinylhomoserine (thiol)-lyase
Gene namesi
Name:CGS1Curated
Synonyms:CYS1Curated, MTO11 Publication
Ordered Locus Names:At3g01120
ORF Names:T4P13.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G01120
TAIRilocus:2102072 AT3G01120

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi77R → H in mto1-4; over-accumulation of soluble methionine. 1 Publication1
Mutagenesisi78R → K in mto1-7; over-accumulation of soluble methionine. 1 Publication1
Mutagenesisi81S → N in mto1-2; over-accumulation of soluble methionine. 1 Publication1
Mutagenesisi84G → D in mto1-3 and mto1-5; over-accumulation of soluble methionine. 1 Publication1
Mutagenesisi84G → S in mto1-1; over-accumulation of soluble methionine. 1 Publication1
Mutagenesisi86A → V in mto1-6; over-accumulation of soluble methionine. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 68Chloroplast1 PublicationAdd BLAST68
ChainiPRO_000003345569 – 563Cystathionine gamma-synthase 1, chloroplasticAdd BLAST495

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei379N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP55217
PRIDEiP55217

Expressioni

Inductioni

Down-regulated by methionine.1 Publication

Gene expression databases

ExpressionAtlasiP55217 baseline and differential
GenevisibleiP55217 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G01120.1

Structurei

3D structure databases

ProteinModelPortaliP55217
SMRiP55217
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi104 – 136Ala-richSequence analysisAdd BLAST33

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0053 Eukaryota
COG0626 LUCA
HOGENOMiHOG000246415
InParanoidiP55217
KOiK01739
OMAiNPRFNIP
OrthoDBiEOG093609C5
PhylomeDBiP55217

Family and domain databases

CDDicd00614 CGS_like, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
InterProiView protein in InterPro
IPR000277 Cys/Met-Metab_PyrdxlP-dep_enz
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF01053 Cys_Met_Meta_PP, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00868 CYS_MET_METAB_PP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55217-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSSFQCPT IFSSSSISGF QCRSDPDLVG SPVGGSSRRR VHASAGISSS
60 70 80 90 100
FTGDAGLSSR ILRFPPNFVR QLSIKARRNC SNIGVAQIVA AKWSNNPSSA
110 120 130 140 150
LPSAAAAAAT SSASAVSSAA SAAAASSAAA APVAAAPPVV LKSVDEEVVV
160 170 180 190 200
AEEGIREKIG SVQLTDSKHS FLSSDGSLTV HAGERLGRGI VTDAITTPVV
210 220 230 240 250
NTSAYFFKKT AELIDFKEKR SVSFEYGRYG NPTTVVLEDK ISALEGAEST
260 270 280 290 300
LVMASGMCAS TVMLLALVPA GGHIVTTTDC YRKTRIFMEN FLPKLGITVT
310 320 330 340 350
VIDPADIAGL EAAVNEFKVS LFFTESPTNP FLRCVDIELV SKICHKRGTL
360 370 380 390 400
VCIDGTFATP LNQKALALGA DLVVHSATKY IGGHNDVLAG CICGSLKLVS
410 420 430 440 450
EIRNLHHVLG GTLNPNAAYL IIRGMKTLHL RVQQQNSTAF RMAEILEAHP
460 470 480 490 500
KVSHVYYPGL PSHPEHELAK RQMTGFGGVV SFEIDGDIET TIKFVDSLKI
510 520 530 540 550
PYIAPSFGGC ESIVDQPAIM SYWDLPQEER LKYGIKDNLV RFSFGVEDFE
560
DVKADILQAL EAI
Length:563
Mass (Da):59,919
Last modified:June 20, 2002 - v3
Checksum:i4FB902896C062860
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102P → T in CAA64383 (Ref. 4) Curated1
Sequence conflicti177S → Q in CAA64383 (Ref. 4) Curated1
Sequence conflicti547E → K in AAM13883 (PubMed:14593172).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti8C → S3 Publications1
Natural varianti55A → G3 Publications1
Natural varianti91A → G3 Publications1
Natural varianti412T → P3 Publications1
Natural varianti459G → A1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43709 mRNA Translation: AAC49574.1
U83500 mRNA Translation: AAB41235.1
AB010888 Genomic DNA Translation: BAA24699.1
AF039206 Genomic DNA Translation: AAC25687.1
X94756 mRNA Translation: CAA64383.1
AC008261 Genomic DNA Translation: AAF26162.1
CP002686 Genomic DNA Translation: AEE73612.1
AY094438 mRNA Translation: AAM19810.1
AY091062 mRNA Translation: AAM13883.1
BT002753 mRNA Translation: AAO22582.1
X79707 Genomic DNA Translation: CAA56143.1
PIRiS51579
S71228
RefSeqiNP_186761.1, NM_110977.3
UniGeneiAt.20198

Genome annotation databases

EnsemblPlantsiAT3G01120.1; AT3G01120.1; AT3G01120
GeneIDi821292
GrameneiAT3G01120.1; AT3G01120.1; AT3G01120
KEGGiath:AT3G01120

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCGS1_ARATH
AccessioniPrimary (citable) accession number: P55217
Secondary accession number(s): P92944, P93038, Q42550
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 20, 2002
Last modified: April 25, 2018
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health