Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P55215

- CASP2_RAT

UniProt

P55215 - CASP2_RAT

Protein

Caspase-2

Gene

Casp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (06 Dec 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival By similarity.By similarity

    Catalytic activityi

    Strict requirement for an Asp residue at P1, with 316-asp being essential for proteolytic activity and has a preferred cleavage sequence of Val-Asp-Val-Ala-Asp-|-.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei277 – 2771By similarity
    Active sitei320 – 3201By similarity

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: RefGenome
    2. cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl

    GO - Biological processi

    1. aging Source: RGD
    2. apoptotic process Source: RGD
    3. brain development Source: RGD
    4. cellular response to mechanical stimulus Source: Ensembl
    5. DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Ensembl
    6. ectopic germ cell programmed cell death Source: Ensembl
    7. extrinsic apoptotic signaling pathway in absence of ligand Source: RefGenome
    8. intrinsic apoptotic signaling pathway in response to DNA damage Source: RefGenome
    9. luteolysis Source: RGD
    10. neural retina development Source: RGD
    11. positive regulation of apoptotic signaling pathway Source: Ensembl
    12. positive regulation of neuron apoptotic process Source: RGD
    13. programmed cell death Source: RGD
    14. protein processing Source: Ensembl
    15. proteolysis Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Apoptosis

    Enzyme and pathway databases

    BRENDAi3.4.22.55. 5301.

    Protein family/group databases

    MEROPSiC14.006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Caspase-2 (EC:3.4.22.55)
    Short name:
    CASP-2
    Alternative name(s):
    Protease ICH-1
    Cleaved into the following 3 chains:
    Gene namesi
    Name:Casp2
    Synonyms:Ich1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi69274. Casp2.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: RGD
    2. mitochondrion Source: RGD
    3. nucleus Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Propeptidei2 – 169168PRO_0000043403Add
    BLAST
    Chaini170 – 325156Caspase-2 subunit p18By similarityPRO_0000044573Add
    BLAST
    Propeptidei326 – 3338PRO_0000044574
    Chaini334 – 452119Caspase-2 subunit p13By similarityPRO_0000004551Add
    BLAST
    Chaini348 – 452105Caspase-2 subunit p12By similarityPRO_0000004552Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei340 – 3401PhosphoserineBy similarity

    Post-translational modificationi

    The mature protease can process its own propeptide, but not that of other caspases.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Zymogen

    Proteomic databases

    PaxDbiP55215.

    PTM databases

    PhosphoSiteiP55215.

    Expressioni

    Gene expression databases

    ArrayExpressiP55215.
    GenevestigatoriP55215.

    Interactioni

    Subunit structurei

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-48602N.
    STRINGi10116.ENSRNOP00000022672.

    Structurei

    3D structure databases

    ProteinModelPortaliP55215.
    SMRiP55215. Positions 173-333, 355-452.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 12190CARDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C14A family.Curated
    Contains 1 CARD domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG305976.
    GeneTreeiENSGT00700000104134.
    HOGENOMiHOG000290714.
    HOVERGENiHBG103962.
    InParanoidiP55215.
    KOiK02186.
    OMAiPCLQVKP.
    OrthoDBiEOG7TTQ7K.
    PhylomeDBiP55215.
    TreeFamiTF102023.

    Family and domain databases

    Gene3Di1.10.533.10. 1 hit.
    3.40.50.1460. 1 hit.
    InterProiIPR001315. CARD.
    IPR029030. Caspase-like_dom.
    IPR017350. Caspase_ICE-type.
    IPR011029. DEATH-like_dom.
    IPR011600. Pept_C14_caspase.
    IPR001309. Pept_C14_ICE_p20.
    IPR016129. Pept_C14_ICE_p20_AS.
    IPR002138. Pept_C14_p10.
    IPR015917. Pept_C14A_p45_core.
    [Graphical view]
    PfamiPF00619. CARD. 1 hit.
    PF00656. Peptidase_C14. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038001. Caspase_ICE. 1 hit.
    PRINTSiPR00376. IL1BCENZYME.
    SMARTiSM00114. CARD. 1 hit.
    SM00115. CASc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47986. SSF47986. 1 hit.
    PROSITEiPS50209. CARD. 1 hit.
    PS01122. CASPASE_CYS. 1 hit.
    PS01121. CASPASE_HIS. 1 hit.
    PS50207. CASPASE_P10. 1 hit.
    PS50208. CASPASE_P20. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P55215-1) [UniParc]FASTAAdd to Basket

    Also known as: caspase-2L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAASSGRSQS SLHRKGLMAA DRRSRILAVC GMHPDHQETL KKNRVVLAKQ    50
    LLLSELLEHL LEKDIITLEM RELIQAKGGS FSQNVELLNL LPKRGPQAFD 100
    AFCEALRETR QGHLEDLLLT TLSDIQHILP PLSCDYDSSL PFSVCESCPP 150
    HKQSRLSTDT MEHSLDNGDG PPCLQVKPCT PEFYQAHYQL AYRLQSQPRG 200
    LALVMSNVHF TGEKDLEFRS GGDVDHTTLV TLFKLLGYNV HVLYDQTAQE 250
    MQEKLQNFAQ LPAHRVTDSC IVALLSHGVE GGIYGVDGKL LQLQEVFRLF 300
    DNANCPSLQN KPKMFFIQAC RGDETDRGVD QQDGKNHAQS PGCEESDAGK 350
    EELMKMRLPT RSDMICGYAC LKGNAAMRNT KRGSWYIEAL TQVFSERACD 400
    MHVADMLVKV NALIKEREGY APGTEFHRCK EMSEYCSTLC QQLYLFPGYP 450
    PT 452
    Length:452
    Mass (Da):50,728
    Last modified:December 6, 2005 - v3
    Checksum:i03F9D096BB741CE3
    GO
    Isoform 2 (identifier: P55215-2) [UniParc]FASTAAdd to Basket

    Also known as: caspase-2S

    The sequence of this isoform differs from the canonical sequence as follows:
         323-343: DETDRGVDQQDGKNHAQSPGC → GAIGSLGPLLLFTAATASLAL
         344-452: Missing.

    Show »
    Length:343
    Mass (Da):38,004
    Checksum:i0292D6130C63F641
    GO

    Sequence cautioni

    The sequence AAB82567.1 differs from that shown. Reason: Frameshift at positions 137 and 189.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti145 – 1451C → Y in AAB82567. (PubMed:9530276)Curated
    Sequence conflicti166 – 1661D → H in AAB82567. (PubMed:9530276)Curated
    Sequence conflicti177 – 1771K → E in AAB82567. (PubMed:9530276)Curated
    Sequence conflicti197 – 1971Q → R in AAB82567. (PubMed:9530276)Curated
    Sequence conflicti340 – 3401S → P in AAC52260. (PubMed:7588240)Curated
    Sequence conflicti348 – 3492AG → TV in AAC52260. (PubMed:7588240)Curated
    Sequence conflicti367 – 3671G → V in AAC52260. (PubMed:7588240)Curated
    Sequence conflicti373 – 3731G → D in AAC52260. (PubMed:7588240)Curated
    Sequence conflicti376 – 3772AM → PI in AAC52260. (PubMed:7588240)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei323 – 34321DETDR…QSPGC → GAIGSLGPLLLFTAATASLA L in isoform 2. 1 PublicationVSP_016555Add
    BLAST
    Alternative sequencei344 – 452109Missing in isoform 2. 1 PublicationVSP_016556Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77933 mRNA. Translation: AAB96379.1.
    AF136231 mRNA. Translation: AAD33684.1.
    AF136232 mRNA. Translation: AAD33685.1.
    AF025671 mRNA. Translation: AAB82567.1. Frameshift.
    U34684 mRNA. Translation: AAC52260.1.
    PIRiI67436.
    JC6507.
    RefSeqiNP_071967.2. NM_022522.2. [P55215-1]
    UniGeneiRn.1438.

    Genome annotation databases

    EnsembliENSRNOT00000022672; ENSRNOP00000022672; ENSRNOG00000016707. [P55215-1]
    GeneIDi64314.
    KEGGirno:64314.
    UCSCiRGD:69274. rat. [P55215-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77933 mRNA. Translation: AAB96379.1 .
    AF136231 mRNA. Translation: AAD33684.1 .
    AF136232 mRNA. Translation: AAD33685.1 .
    AF025671 mRNA. Translation: AAB82567.1 . Frameshift.
    U34684 mRNA. Translation: AAC52260.1 .
    PIRi I67436.
    JC6507.
    RefSeqi NP_071967.2. NM_022522.2. [P55215-1 ]
    UniGenei Rn.1438.

    3D structure databases

    ProteinModelPortali P55215.
    SMRi P55215. Positions 173-333, 355-452.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48602N.
    STRINGi 10116.ENSRNOP00000022672.

    Protein family/group databases

    MEROPSi C14.006.

    PTM databases

    PhosphoSitei P55215.

    Proteomic databases

    PaxDbi P55215.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000022672 ; ENSRNOP00000022672 ; ENSRNOG00000016707 . [P55215-1 ]
    GeneIDi 64314.
    KEGGi rno:64314.
    UCSCi RGD:69274. rat. [P55215-1 ]

    Organism-specific databases

    CTDi 835.
    RGDi 69274. Casp2.

    Phylogenomic databases

    eggNOGi NOG305976.
    GeneTreei ENSGT00700000104134.
    HOGENOMi HOG000290714.
    HOVERGENi HBG103962.
    InParanoidi P55215.
    KOi K02186.
    OMAi PCLQVKP.
    OrthoDBi EOG7TTQ7K.
    PhylomeDBi P55215.
    TreeFami TF102023.

    Enzyme and pathway databases

    BRENDAi 3.4.22.55. 5301.

    Miscellaneous databases

    NextBioi 612988.
    PROi P55215.

    Gene expression databases

    ArrayExpressi P55215.
    Genevestigatori P55215.

    Family and domain databases

    Gene3Di 1.10.533.10. 1 hit.
    3.40.50.1460. 1 hit.
    InterProi IPR001315. CARD.
    IPR029030. Caspase-like_dom.
    IPR017350. Caspase_ICE-type.
    IPR011029. DEATH-like_dom.
    IPR011600. Pept_C14_caspase.
    IPR001309. Pept_C14_ICE_p20.
    IPR016129. Pept_C14_ICE_p20_AS.
    IPR002138. Pept_C14_p10.
    IPR015917. Pept_C14A_p45_core.
    [Graphical view ]
    Pfami PF00619. CARD. 1 hit.
    PF00656. Peptidase_C14. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038001. Caspase_ICE. 1 hit.
    PRINTSi PR00376. IL1BCENZYME.
    SMARTi SM00114. CARD. 1 hit.
    SM00115. CASc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47986. SSF47986. 1 hit.
    PROSITEi PS50209. CARD. 1 hit.
    PS01122. CASPASE_CYS. 1 hit.
    PS01121. CASPASE_HIS. 1 hit.
    PS50207. CASPASE_P10. 1 hit.
    PS50208. CASPASE_P20. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the cDNA encoding rat caspase-2."
      Sato N., Milligan C.E., Uchiyama Y., Oppenheim R.W.
      Gene 202:127-132(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Two caspase-2 transcripts are expressed in rat hippocampus after global cerebral ischemia."
      Jin K., Nagayama T., Mao X., Kawaguchi K., Hickey R.W., Greenberg D.A., Simon R.P., Graham S.H.
      J. Neurochem. 81:25-35(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Strain: Sprague-Dawley.
      Tissue: Brain.
    3. "Identification of gene family of caspases in rat kidney and altered expression in ischemia-reperfusion injury."
      Kaushal G.P., Singh A.B., Shah S.V.
      Am. J. Physiol. 274:F587-F595(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 67-324.
      Strain: Sprague-Dawley.
      Tissue: Kidney cortex.
    4. "Interleukin-1 beta-converting enzyme-related proteases (IRPs) and mammalian cell death: dissociation of IRP-induced oligonucleosomal endonuclease activity from morphological apoptosis in granulosa cells of the ovarian follicle."
      Flaws J.A., Kugu K., Trbovich A.M., Desanti A., Tilly K.I., Hirshfield A.N., Tilly J.L.
      Endocrinology 136:5042-5053(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 198-379.
      Tissue: Ovary.

    Entry informationi

    Entry nameiCASP2_RAT
    AccessioniPrimary (citable) accession number: P55215
    Secondary accession number(s): O35398, O55194, Q9WUI6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3