P55209 (NP1L1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleosome assembly protein 1-like 1

Alternative name(s):
NAP-1-related protein
Short name=hNRP

Gene names

Name:	NAP1L1
Synonyms:	NRP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in modulating chromatin formation and contribute to regulation of cell proliferation.
Subcellular location	Nucleus. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.7
Tissue specificity	Ubiquitously expressed.
Domain	The acidic domains are probably involved in the interaction with histones.
Post-translational modification	Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human By similarity.
Sequence similarities	Belongs to the nucleosome assembly protein (NAP) family.

Ontologies

Keywords
Cellular component	Nucleus
PTM	Acetylation Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Traceable author statement Ref.1. Source: ProtInc nucleosome assembly Traceable author statement Ref.1. Source: ProtInc positive regulation of cell proliferation Traceable author statement Ref.1. Source: ProtInc
Cellular_component	melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CREBBP	Q92793	3	EBI-356392,EBI-81215
EP300	Q09472	3	EBI-356392,EBI-447295
NR2F6	P10588	2	EBI-356392,EBI-2681496

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Nucleosome assembly protein 1-like 1		PRO_0000185652
	Propeptide	389 – 391	3	Removed in mature form Probable		PRO_0000393943
Regions
	Motif	273 – 279	7	Nuclear localization signal Potential
	Compositional bias	10 – 30	21	Asp/Glu-rich (acidic)
	Compositional bias	129 – 145	17	Asp/Glu-rich (acidic)
	Compositional bias	348 – 378	31	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	10	1	Phosphoserine Ref.10
	Modified residue	62	1	Phosphothreonine Ref.8 Ref.10 Ref.12 Ref.14 Ref.16
	Modified residue	69	1	Phosphoserine Ref.10 Ref.12 Ref.14
	Modified residue	116	1	N6-acetyllysine Ref.13
	Modified residue	143	1	Phosphoserine Ref.10
	Modified residue	388	1	Cysteine methyl ester Probable
	Lipidation	388	1	S-farnesyl cysteine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P55209 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: E5B2EAA4EAE551D2	FASTA	391	45,374
	10 20 30 40 50 60 MADIDNKEQS ELDQDLDDVE EVEEEETGEE TKLKARQLTV QMMQNPQILA ALQERLDGLV 70 80 90 100 110 120 ETPTGYIESL PRVVKRRVNA LKNLQVKCAQ IEAKFYEEVH DLERKYAVLY QPLFDKRFEI 130 140 150 160 170 180 INAIYEPTEE ECEWKPDEED EISEELKEKA KIEDEKKDEE KEDPKGIPEF WLTVFKNVDL 190 200 210 220 230 240 LSDMVQEHDE PILKHLKDIK VKFSDAGQPM SFVLEFHFEP NEYFTNEVLT KTYRMRSEPD 250 260 270 280 290 300 DSDPFSFDGP EIMGCTGCQI DWKKGKNVTL KTIKKKQKHK GRGTVRTVTK TVSNDSFFNF 310 320 330 340 350 360 FAPPEVPESG DLDDDAEAIL AADFEIGHFL RERIIPRSVL YFTGEAIEDD DDDYDEEGEE 370 380 390 ADEEGEEEGD EENDPDYDPK KDQNPAECKQ Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of hNRP, a cDNA encoding a human nucleosome-assembly-protein-I-related gene product involved in the induction of cell proliferation." Simon H.-U., Mills G.B., Kozlowski M., Hogg D., Branch D., Ishimi Y., Siminovitch K.A. Biochem. J. 297:389-397(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Thymus.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Melanoma.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[5]	"A tagging-via-substrate technology for detection and proteomics of farnesylated proteins." Kho Y., Kim S.C., Jiang C., Barma D., Kwon S.W., Cheng J., Jaunbergs J., Weinbaum C., Tamanoi F., Falck J., Zhao Y. Proc. Natl. Acad. Sci. U.S.A. 101:12479-12484(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ISOPRENYLATION AT CYS-388.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F. J. Proteome Res. 5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Melanoma.
[8]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-62, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; THR-62; SER-69 AND SER-143, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"The histone chaperone protein Nucleosome Assembly Protein-1 (hNAP-1) binds HIV-1 Tat and promotes viral transcription." Vardabasso C., Manganaro L., Lusic M., Marcello A., Giacca M. Retrovirology 5:8-8(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HIV-1 TAT.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-62 AND SER-69, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-116, MASS SPECTROMETRY.
[14]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-62 AND SER-69, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-62, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86667 mRNA. Translation: AAC37544.1. BT007023 mRNA. Translation: AAP35669.1. AL162068 mRNA. Translation: CAB82405.1. BC002387 mRNA. Translation: AAH02387.1.
IPI	IPI00023860.
PIR	S40510.
RefSeq	NP_004528.1. NM_004537.4. NP_631946.1. NM_139207.2.
UniGene	Hs.524599. Hs.695185.
3D structure databases
ProteinModelPortal	P55209.
ModBase	Search...
Protein-protein interaction databases
IntAct	P55209. 39 interactions.
MINT	MINT-150144.
STRING	9606.ENSP00000261182.
PTM databases
PhosphoSite	P55209.
Polymorphism databases
DMDM	1709337.
2D gel databases
OGP	P55209.
Proteomic databases
PaxDb	P55209.
PRIDE	P55209.
Protocols and materials databases
DNASU	4673.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261182; ENSP00000261182; ENSG00000187109. ENST00000393263; ENSP00000376947; ENSG00000187109.
GeneID	4673.
KEGG	hsa:4673.
UCSC	uc001sxw.2. human.
Organism-specific databases
CTD	4673.
GeneCards	GC12M076438.
HGNC	HGNC:7637. NAP1L1.
HPA	HPA028861.
MIM	164060. gene.
neXtProt	NX_P55209.
PharmGKB	PA31439.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG285183.
HOGENOM	HOG000171827.
HOVERGEN	HBG052653.
InParanoid	P55209.
KO	K11279.
OMA	QCAQIEA.
OrthoDB	EOG46WZ99.
PhylomeDB	P55209.
Gene expression databases
ArrayExpress	P55209.
Bgee	P55209.
CleanEx	HS_NAP1L1.
Genevestigator	P55209.
GermOnline	ENSG00000187109. Homo sapiens.
Family and domain databases
InterPro	IPR002164. NAP_family. [Graphical view]
PANTHER	PTHR11875. PTHR11875. 1 hit.
Pfam	PF00956. NAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NAP1L1. human.
GenomeRNAi	4673.
NextBio	18010.
PMAP-CutDB	P55209.
SOURCE	Search...

Entry information

Entry name

NP1L1_HUMAN

Accession

Primary (citable) accession number: P55209

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.