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Protein

Guanylate cyclase 2G

Gene

Gucy2g

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Binds to a yet not identified ligand.
Possible role in processes such as water and electrolyte balance, and muscle contraction.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. GTP binding Source: UniProtKB-KW
  3. guanylate cyclase activity Source: RGD
  4. protein kinase activity Source: InterPro

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Receptor

Keywords - Biological processi

cGMP biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate cyclase 2G (EC:4.6.1.2)
Alternative name(s):
Guanylyl cyclase receptor G
Short name:
GC-G
Kinase-like domain-containing soluble guanylyl cyclase
Short name:
ksGC
Gene namesi
Name:Gucy2g
Synonyms:Ksgc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621853. Gucy2g.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini44 – 481438ExtracellularSequence AnalysisAdd
BLAST
Transmembranei482 – 50221HelicalSequence AnalysisAdd
BLAST
Topological domaini503 – 1100598CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4343Sequence AnalysisAdd
BLAST
Chaini44 – 11001057Guanylate cyclase 2GPRO_0000042179Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi55 – 551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi418 – 4181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi443 – 4431N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP55205.
PRIDEiP55205.

Expressioni

Tissue specificityi

Expressed in lung, kidney and skeletal muscle. Low levels in intestine.1 Publication

Gene expression databases

GenevestigatoriP55205.

Interactioni

Subunit structurei

Homodimer or heterodimer with NPR1.By similarity

Structurei

3D structure databases

ProteinModelPortaliP55205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini549 – 826278Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini901 – 1031131Guanylate cyclasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2114.
HOGENOMiHOG000293307.
HOVERGENiHBG051862.
InParanoidiP55205.
PhylomeDBiP55205.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR001170. Ntpep_rcpt.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00255. NATPEPTIDER.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P55205-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASRARSEPP LEHRFYGGAE SHAGHSSLVL TLFVVMLMTC LEAAKLTVGF
60 70 80 90 100
HAPWNISHPF SVQRLGAGLQ IAVDKLNSEP VGPGNLSWEF TYTNATCNAK
110 120 130 140 150
ESLAAFIDQV QREHISVLIG PACPEAAEVI GLLASEWDIP LFDFVGQMTA
160 170 180 190 200
LEDHFWCDTC VTLVPPKQEI GTVLRESLQY LGWEYIGVFG GSSAGSSWGE
210 220 230 240 250
VNELWKAVED ELQLHFTITA RVRYSSGHSD LLQEGLRSMS SVARVIILIC
260 270 280 290 300
SSEDAKHILQ AAEDLGLNSG EFVFLLLQQL EDSFWKEVLA EDKVTRFPKV
310 320 330 340 350
YESVFLIAPS TYGGSAGDDD FRKQVYQRLR RPPFQSSISS EDQVSPYSAY
360 370 380 390 400
LHDALLLYAQ TVEEMMKAEK DFRDGRQLIS TLRADQVTLQ GITGPVLLDA
410 420 430 440 450
QGKRHMDYSV YALQKSGNGS RFLPFLHYDS FQKVIRPWRD DLNASGPHGS
460 470 480 490 500
HPEYKPDCGF HEDLCRTKPP TGAGMTASVT AVIPTVTLLV VASAAAITGL
510 520 530 540 550
MLWRLRGKVQ NHPGDTWWQI HYDSITLLPQ HKPSHRGTPM SRCNVSNAST
560 570 580 590 600
VKISADCGSF AKTHQDEELF YAPVGLYQGN HVALCYIGEE AEARIKKPTV
610 620 630 640 650
LREVWLMCEL KHENIVPFFG VCTEPPNICI VTQYCKKGSL KDVLRNSDHE
660 670 680 690 700
MDWIFKLSFV YDIVNGMLFL HGSPLRSHGN LKPSNCLVDS HMQLKLAGFG
710 720 730 740 750
LWEFKHGSTC RIYNQEATDH SELYWTAPEL LRLRELPWSG TPQGDVYSFA
760 770 780 790 800
ILLRDLIHQQ AHGPFEDLEA APEEIISCIK DPRAPVPLRP SLLEDKGDER
810 820 830 840 850
IVALVRACWA ESPEQRPAFP SIKKTLREAS PRGRVSILDS MMGKLEMYAS
860 870 880 890 900
HLEEVVEERT CQLVAEKRKV EKLLSTMLPS FVGEQLIAGK SVEPEHFESV
910 920 930 940 950
TIFFSDIVGF TKLCSLSSPL QVVKLLNDLY SLFDHTIQTH DVYKVETIGD
960 970 980 990 1000
AYMVASGLPI RNGAQHADEI ATMSLHLLSV TTNFQIGHMP EERLKLRIGL
1010 1020 1030 1040 1050
HTGPVVAGVV GITMPRYCLF GDTVNMASRM ESSSLPLRIH VSQSTARALL
1060 1070 1080 1090 1100
VAGGYHLQKR GTISVKGKGE QTTFWLTGKD GFAVPLPEFT EEEAKVPEIL
Length:1,100
Mass (Da):122,497
Last modified:September 27, 2005 - v2
Checksum:iE487701031EF9965
GO
Isoform 2 (identifier: P55205-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-666: Missing.

Show »
Length:434
Mass (Da):48,319
Checksum:iB4829C053CEFAE31
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti738 – 7447WSGTPQG → GPAPRR in AAC52417 (PubMed:8554626).Curated
Sequence conflicti782 – 7821P → S in AAC52417 (PubMed:8554626).Curated
Sequence conflicti786 – 7861V → F in AAC52417 (PubMed:8554626).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 666666Missing in isoform 2. 1 PublicationVSP_015787Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024622 mRNA. Translation: AAC01752.1.
U33847 mRNA. Translation: AAC52417.1.
PIRiT42260.
UniGeneiRn.10198.

Genome annotation databases

UCSCiRGD:621853. rat. [P55205-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024622 mRNA. Translation: AAC01752.1.
U33847 mRNA. Translation: AAC52417.1.
PIRiT42260.
UniGeneiRn.10198.

3D structure databases

ProteinModelPortaliP55205.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP55205.
PRIDEiP55205.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621853. rat. [P55205-1]

Organism-specific databases

RGDi621853. Gucy2g.

Phylogenomic databases

eggNOGiCOG2114.
HOGENOMiHOG000293307.
HOVERGENiHBG051862.
InParanoidiP55205.
PhylomeDBiP55205.

Miscellaneous databases

NextBioi623135.

Gene expression databases

GenevestigatoriP55205.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR001170. Ntpep_rcpt.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00255. NATPEPTIDER.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A new type soluble guanylyl cyclase, which contains a kinase-like domain: its structure and expression."
    Kojima M., Hisaki K., Matsuo H., Kangawa K.
    Biochem. Biophys. Res. Commun. 217:993-1000(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. "The cloning and expression of a new guanylyl cyclase orphan receptor."
    Schulz S., Wedel B.J., Matthews A., Garbers D.L.
    J. Biol. Chem. 273:1032-1037(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).

Entry informationi

Entry nameiGUC2G_RAT
AccessioniPrimary (citable) accession number: P55205
Secondary accession number(s): O54884
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 27, 2005
Last modified: January 7, 2015
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.