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P55205 (GUC2G_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Guanylate cyclase 2G
EC=4.6.1.2
Alternative name(s):
Guanylyl cyclase receptor G
Short name=GC-G
Kinase-like domain-containing soluble guanylyl cyclase
Short name=ksGC
Gene names
Name:Gucy2g
Synonyms:Ksgc
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length1100 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds to a yet not identified ligand.

Possible role in processes such as water and electrolyte balance, and muscle contraction.

Catalytic activity

GTP = 3',5'-cyclic GMP + diphosphate.

Subunit structure

Homodimer or heterodimer with NPR1 By similarity.

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein Potential.

Isoform 2: Cytoplasm Potential.

Tissue specificity

Expressed in lung, kidney and skeletal muscle. Low levels in intestine. Ref.1

Sequence similarities

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Contains 1 guanylate cyclase domain.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processcGMP biosynthesis
   Cellular componentCell membrane
Cytoplasm
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   LigandGTP-binding
Nucleotide-binding
   Molecular functionLyase
Receptor
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processintracellular signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: InterPro

GTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

guanylate cyclase activity

Traceable author statement Ref.2. Source: RGD

protein kinase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P55205-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P55205-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-666: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4343 Potential
Chain44 – 11001057Guanylate cyclase 2G
PRO_0000042179

Regions

Topological domain44 – 481438Extracellular Potential
Transmembrane482 – 50221Helical; Potential
Topological domain503 – 1100598Cytoplasmic Potential
Domain549 – 826278Protein kinase
Domain901 – 1031131Guanylate cyclase

Amino acid modifications

Glycosylation551N-linked (GlcNAc...) Potential
Glycosylation851N-linked (GlcNAc...) Potential
Glycosylation941N-linked (GlcNAc...) Potential
Glycosylation4181N-linked (GlcNAc...) Potential
Glycosylation4431N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 666666Missing in isoform 2.
VSP_015787

Experimental info

Sequence conflict738 – 7447WSGTPQG → GPAPRR in AAC52417. Ref.1
Sequence conflict7821P → S in AAC52417. Ref.1
Sequence conflict7861V → F in AAC52417. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 27, 2005. Version 2.
Checksum: E487701031EF9965

FASTA1,100122,497
        10         20         30         40         50         60 
MASRARSEPP LEHRFYGGAE SHAGHSSLVL TLFVVMLMTC LEAAKLTVGF HAPWNISHPF 

        70         80         90        100        110        120 
SVQRLGAGLQ IAVDKLNSEP VGPGNLSWEF TYTNATCNAK ESLAAFIDQV QREHISVLIG 

       130        140        150        160        170        180 
PACPEAAEVI GLLASEWDIP LFDFVGQMTA LEDHFWCDTC VTLVPPKQEI GTVLRESLQY 

       190        200        210        220        230        240 
LGWEYIGVFG GSSAGSSWGE VNELWKAVED ELQLHFTITA RVRYSSGHSD LLQEGLRSMS 

       250        260        270        280        290        300 
SVARVIILIC SSEDAKHILQ AAEDLGLNSG EFVFLLLQQL EDSFWKEVLA EDKVTRFPKV 

       310        320        330        340        350        360 
YESVFLIAPS TYGGSAGDDD FRKQVYQRLR RPPFQSSISS EDQVSPYSAY LHDALLLYAQ 

       370        380        390        400        410        420 
TVEEMMKAEK DFRDGRQLIS TLRADQVTLQ GITGPVLLDA QGKRHMDYSV YALQKSGNGS 

       430        440        450        460        470        480 
RFLPFLHYDS FQKVIRPWRD DLNASGPHGS HPEYKPDCGF HEDLCRTKPP TGAGMTASVT 

       490        500        510        520        530        540 
AVIPTVTLLV VASAAAITGL MLWRLRGKVQ NHPGDTWWQI HYDSITLLPQ HKPSHRGTPM 

       550        560        570        580        590        600 
SRCNVSNAST VKISADCGSF AKTHQDEELF YAPVGLYQGN HVALCYIGEE AEARIKKPTV 

       610        620        630        640        650        660 
LREVWLMCEL KHENIVPFFG VCTEPPNICI VTQYCKKGSL KDVLRNSDHE MDWIFKLSFV 

       670        680        690        700        710        720 
YDIVNGMLFL HGSPLRSHGN LKPSNCLVDS HMQLKLAGFG LWEFKHGSTC RIYNQEATDH 

       730        740        750        760        770        780 
SELYWTAPEL LRLRELPWSG TPQGDVYSFA ILLRDLIHQQ AHGPFEDLEA APEEIISCIK 

       790        800        810        820        830        840 
DPRAPVPLRP SLLEDKGDER IVALVRACWA ESPEQRPAFP SIKKTLREAS PRGRVSILDS 

       850        860        870        880        890        900 
MMGKLEMYAS HLEEVVEERT CQLVAEKRKV EKLLSTMLPS FVGEQLIAGK SVEPEHFESV 

       910        920        930        940        950        960 
TIFFSDIVGF TKLCSLSSPL QVVKLLNDLY SLFDHTIQTH DVYKVETIGD AYMVASGLPI 

       970        980        990       1000       1010       1020 
RNGAQHADEI ATMSLHLLSV TTNFQIGHMP EERLKLRIGL HTGPVVAGVV GITMPRYCLF 

      1030       1040       1050       1060       1070       1080 
GDTVNMASRM ESSSLPLRIH VSQSTARALL VAGGYHLQKR GTISVKGKGE QTTFWLTGKD 

      1090       1100 
GFAVPLPEFT EEEAKVPEIL

« Hide

Isoform 2 [UniParc].

Checksum: B4829C053CEFAE31
Show »

FASTA43448,319

References

[1]"A new type soluble guanylyl cyclase, which contains a kinase-like domain: its structure and expression."
Kojima M., Hisaki K., Matsuo H., Kangawa K.
Biochem. Biophys. Res. Commun. 217:993-1000(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Kidney.
[2]"The cloning and expression of a new guanylyl cyclase orphan receptor."
Schulz S., Wedel B.J., Matthews A., Garbers D.L.
J. Biol. Chem. 273:1032-1037(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF024622 mRNA. Translation: AAC01752.1.
U33847 mRNA. Translation: AAC52417.1.
IPIIPI00337183.
IPI00654433.
PIRT42260.
UniGeneRn.10198.

3D structure databases

ProteinModelPortalP55205.
ModBaseSearch...

Proteomic databases

PaxDbP55205.
PRIDEP55205.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:621853. rat.

Organism-specific databases

RGD621853. Gucy2g.

Phylogenomic databases

eggNOGCOG2114.
HOGENOMHOG000293307.
HOVERGENHBG051862.
InParanoidP55205.
OrthoDBEOG4TQM89.

Gene expression databases

ArrayExpressP55205.
GenevestigatorP55205.
GermOnlineENSRNOG00000015724. Rattus norvegicus.

Family and domain databases

Gene3D3.30.70.1230. 1 hit.
InterProIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR001170. Ntpep_rcpt.
IPR000719. Prot_kinase_cat_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSPR00255. NATPEPTIDER.
SMARTSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMSSF55073. A/G_cyclase. 1 hit.
SSF56112. Kinase_like. 1 hit.
PROSITEPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio623135.

Entry information

Entry nameGUC2G_RAT
AccessionPrimary (citable) accession number: P55205
Secondary accession number(s): O54884
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 27, 2005
Last modified: April 3, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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