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P55205

- GUC2G_RAT

UniProt

P55205 - GUC2G_RAT

Protein

Guanylate cyclase 2G

Gene

Gucy2g

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (27 Sep 2005)
      Previous versions | rss
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    Functioni

    Binds to a yet not identified ligand.
    Possible role in processes such as water and electrolyte balance, and muscle contraction.

    Catalytic activityi

    GTP = 3',5'-cyclic GMP + diphosphate.

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. guanylate cyclase activity Source: RGD
    4. protein kinase activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro

    Keywords - Molecular functioni

    Lyase, Receptor

    Keywords - Biological processi

    cGMP biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanylate cyclase 2G (EC:4.6.1.2)
    Alternative name(s):
    Guanylyl cyclase receptor G
    Short name:
    GC-G
    Kinase-like domain-containing soluble guanylyl cyclase
    Short name:
    ksGC
    Gene namesi
    Name:Gucy2g
    Synonyms:Ksgc
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621853. Gucy2g.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4343Sequence AnalysisAdd
    BLAST
    Chaini44 – 11001057Guanylate cyclase 2GPRO_0000042179Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi55 – 551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi418 – 4181N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi443 – 4431N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP55205.
    PRIDEiP55205.

    Expressioni

    Tissue specificityi

    Expressed in lung, kidney and skeletal muscle. Low levels in intestine.1 Publication

    Gene expression databases

    GenevestigatoriP55205.

    Interactioni

    Subunit structurei

    Homodimer or heterodimer with NPR1.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP55205.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini44 – 481438ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini503 – 1100598CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei482 – 50221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini549 – 826278Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini901 – 1031131Guanylate cyclasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
    Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2114.
    HOGENOMiHOG000293307.
    HOVERGENiHBG051862.
    InParanoidiP55205.
    PhylomeDBiP55205.

    Family and domain databases

    Gene3Di3.30.70.1230. 1 hit.
    InterProiIPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR001828. ANF_lig-bd_rcpt.
    IPR011009. Kinase-like_dom.
    IPR001170. Ntpep_rcpt.
    IPR028082. Peripla_BP_I.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF01094. ANF_receptor. 1 hit.
    PF00211. Guanylate_cyc. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    PRINTSiPR00255. NATPEPTIDER.
    SMARTiSM00044. CYCc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.
    SSF55073. SSF55073. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P55205-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASRARSEPP LEHRFYGGAE SHAGHSSLVL TLFVVMLMTC LEAAKLTVGF     50
    HAPWNISHPF SVQRLGAGLQ IAVDKLNSEP VGPGNLSWEF TYTNATCNAK 100
    ESLAAFIDQV QREHISVLIG PACPEAAEVI GLLASEWDIP LFDFVGQMTA 150
    LEDHFWCDTC VTLVPPKQEI GTVLRESLQY LGWEYIGVFG GSSAGSSWGE 200
    VNELWKAVED ELQLHFTITA RVRYSSGHSD LLQEGLRSMS SVARVIILIC 250
    SSEDAKHILQ AAEDLGLNSG EFVFLLLQQL EDSFWKEVLA EDKVTRFPKV 300
    YESVFLIAPS TYGGSAGDDD FRKQVYQRLR RPPFQSSISS EDQVSPYSAY 350
    LHDALLLYAQ TVEEMMKAEK DFRDGRQLIS TLRADQVTLQ GITGPVLLDA 400
    QGKRHMDYSV YALQKSGNGS RFLPFLHYDS FQKVIRPWRD DLNASGPHGS 450
    HPEYKPDCGF HEDLCRTKPP TGAGMTASVT AVIPTVTLLV VASAAAITGL 500
    MLWRLRGKVQ NHPGDTWWQI HYDSITLLPQ HKPSHRGTPM SRCNVSNAST 550
    VKISADCGSF AKTHQDEELF YAPVGLYQGN HVALCYIGEE AEARIKKPTV 600
    LREVWLMCEL KHENIVPFFG VCTEPPNICI VTQYCKKGSL KDVLRNSDHE 650
    MDWIFKLSFV YDIVNGMLFL HGSPLRSHGN LKPSNCLVDS HMQLKLAGFG 700
    LWEFKHGSTC RIYNQEATDH SELYWTAPEL LRLRELPWSG TPQGDVYSFA 750
    ILLRDLIHQQ AHGPFEDLEA APEEIISCIK DPRAPVPLRP SLLEDKGDER 800
    IVALVRACWA ESPEQRPAFP SIKKTLREAS PRGRVSILDS MMGKLEMYAS 850
    HLEEVVEERT CQLVAEKRKV EKLLSTMLPS FVGEQLIAGK SVEPEHFESV 900
    TIFFSDIVGF TKLCSLSSPL QVVKLLNDLY SLFDHTIQTH DVYKVETIGD 950
    AYMVASGLPI RNGAQHADEI ATMSLHLLSV TTNFQIGHMP EERLKLRIGL 1000
    HTGPVVAGVV GITMPRYCLF GDTVNMASRM ESSSLPLRIH VSQSTARALL 1050
    VAGGYHLQKR GTISVKGKGE QTTFWLTGKD GFAVPLPEFT EEEAKVPEIL 1100
    Length:1,100
    Mass (Da):122,497
    Last modified:September 27, 2005 - v2
    Checksum:iE487701031EF9965
    GO
    Isoform 2 (identifier: P55205-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-666: Missing.

    Show »
    Length:434
    Mass (Da):48,319
    Checksum:iB4829C053CEFAE31
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti738 – 7447WSGTPQG → GPAPRR in AAC52417. (PubMed:8554626)Curated
    Sequence conflicti782 – 7821P → S in AAC52417. (PubMed:8554626)Curated
    Sequence conflicti786 – 7861V → F in AAC52417. (PubMed:8554626)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 666666Missing in isoform 2. 1 PublicationVSP_015787Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF024622 mRNA. Translation: AAC01752.1.
    U33847 mRNA. Translation: AAC52417.1.
    PIRiT42260.
    UniGeneiRn.10198.

    Genome annotation databases

    UCSCiRGD:621853. rat. [P55205-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF024622 mRNA. Translation: AAC01752.1 .
    U33847 mRNA. Translation: AAC52417.1 .
    PIRi T42260.
    UniGenei Rn.10198.

    3D structure databases

    ProteinModelPortali P55205.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P55205.
    PRIDEi P55205.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:621853. rat. [P55205-1 ]

    Organism-specific databases

    RGDi 621853. Gucy2g.

    Phylogenomic databases

    eggNOGi COG2114.
    HOGENOMi HOG000293307.
    HOVERGENi HBG051862.
    InParanoidi P55205.
    PhylomeDBi P55205.

    Miscellaneous databases

    NextBioi 623135.

    Gene expression databases

    Genevestigatori P55205.

    Family and domain databases

    Gene3Di 3.30.70.1230. 1 hit.
    InterProi IPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR001828. ANF_lig-bd_rcpt.
    IPR011009. Kinase-like_dom.
    IPR001170. Ntpep_rcpt.
    IPR028082. Peripla_BP_I.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF01094. ANF_receptor. 1 hit.
    PF00211. Guanylate_cyc. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    PRINTSi PR00255. NATPEPTIDER.
    SMARTi SM00044. CYCc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    SSF55073. SSF55073. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new type soluble guanylyl cyclase, which contains a kinase-like domain: its structure and expression."
      Kojima M., Hisaki K., Matsuo H., Kangawa K.
      Biochem. Biophys. Res. Commun. 217:993-1000(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    2. "The cloning and expression of a new guanylyl cyclase orphan receptor."
      Schulz S., Wedel B.J., Matthews A., Garbers D.L.
      J. Biol. Chem. 273:1032-1037(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).

    Entry informationi

    Entry nameiGUC2G_RAT
    AccessioniPrimary (citable) accession number: P55205
    Secondary accession number(s): O54884
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 27, 2005
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3