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P55196

- AFAD_HUMAN

UniProt

P55196 - AFAD_HUMAN

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Protein
Afadin
Gene
MLLT4, AF6
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei26 – 261Breakpoint for translocation to form KMT2A/MLL1-MLLT4

GO - Molecular functioni

  1. protein C-terminus binding Source: ProtInc
  2. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. adherens junction organization Source: Reactome
  2. cell adhesion Source: ProtInc
  3. cell junction assembly Source: Reactome
  4. cell-cell junction organization Source: Reactome
  5. cell-cell signaling Source: ProtInc
  6. signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_19195. Adherens junctions interactions.
SignaLinkiP55196.

Names & Taxonomyi

Protein namesi
Recommended name:
Afadin
Alternative name(s):
ALL1-fused gene from chromosome 6 protein
Short name:
Protein AF-6
Gene namesi
Name:MLLT4
Synonyms:AF6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:7137. MLLT4.

Subcellular locationi

Cell junctionadherens junction
Note: Not found at cell-matrix AJs.

GO - Cellular componenti

  1. apical part of cell Source: Ensembl
  2. cell junction Source: HPA
  3. cell-cell adherens junction Source: Ensembl
  4. cell-cell junction Source: ProtInc
  5. cytoplasm Source: HPA
  6. cytosol Source: Reactome
  7. nucleus Source: HPA
  8. plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cell junction

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with KMT2A/MLL1. The result is a rogue activator protein.

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18241824Afadin
PRO_0000215918Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei216 – 2161Phosphoserine1 Publication
Modified residuei557 – 5571Phosphoserine By similarity
Modified residuei1107 – 11071Phosphoserine1 Publication
Modified residuei1182 – 11821Phosphoserine4 Publications
Modified residuei1232 – 12321Phosphothreonine1 Publication
Modified residuei1721 – 17211Phosphoserine3 Publications
Modified residuei1779 – 17791Phosphoserine2 Publications
Modified residuei1799 – 17991Phosphoserine2 Publications
Modified residuei1807 – 18071N6-acetyllysine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP55196.
PaxDbiP55196.
PRIDEiP55196.

PTM databases

PhosphoSiteiP55196.

Expressioni

Gene expression databases

ArrayExpressiP55196.
BgeeiP55196.
CleanExiHS_MLLT4.
GenevestigatoriP55196.

Organism-specific databases

HPAiCAB013496.
HPA030212.
HPA030213.
HPA030214.
HPA030215.
HPA049868.

Interactioni

Subunit structurei

Homodimer. Interacts with F-actin, nectin and PVRL3/nectin-3. Essential for the association of nectin and E-cadherin. Isoform 1/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1 and RIT2 By similarity. Interacts with NRXN1 and BCR.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DBN1Q166434EBI-365875,EBI-351394
PVRL2Q926922EBI-365875,EBI-718419
SRCP129317EBI-365875,EBI-621482

Protein-protein interaction databases

BioGridi110447. 47 interactions.
IntActiP55196. 22 interactions.
MINTiMINT-90828.
STRINGi9606.ENSP00000383623.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1006 – 10138
Beta strandi1015 – 10173
Beta strandi1020 – 10256
Beta strandi1028 – 10314
Beta strandi1034 – 10407
Beta strandi1042 – 10443
Helixi1045 – 10495
Beta strandi1054 – 10618
Helixi1071 – 10799
Beta strandi1083 – 10919

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T2MNMR-A1003-1094[»]
1XZ9NMR-A1001-1096[»]
2AINNMR-A1003-1094[»]
2EXGNMR-A1001-1096[»]
ProteinModelPortaliP55196.
SMRiP55196. Positions 42-134, 249-348, 374-503, 603-956, 1001-1096, 1521-1687.

Miscellaneous databases

EvolutionaryTraceiP55196.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 13395Ras-associating 1
Add
BLAST
Domaini246 – 348103Ras-associating 2
Add
BLAST
Domaini426 – 49267FHA
Add
BLAST
Domaini668 – 908241Dilute
Add
BLAST
Domaini1007 – 109387PDZ
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili146 – 18540 Reviewed prediction
Add
BLAST
Coiled coili1408 – 144841 Reviewed prediction
Add
BLAST
Coiled coili1523 – 1667145 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi163 – 17513Glu/Lys-rich
Add
BLAST
Compositional biasi1346 – 139247Pro-rich
Add
BLAST
Compositional biasi1578 – 158811Asp/Glu-rich (acidic)
Add
BLAST
Compositional biasi1678 – 170831Pro-rich
Add
BLAST

Domaini

The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich C-terminal domain interacts with F-actin.

Sequence similaritiesi

Contains 1 dilute domain.
Contains 1 FHA domain.
Contains 1 PDZ (DHR) domain.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG291597.
HOVERGENiHBG050463.
KOiK05702.
OMAiLIPHTRS.
PhylomeDBiP55196.
TreeFamiTF350731.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
2.60.200.20. 1 hit.
InterProiIPR018444. Dil_domain.
IPR002710. Dilute.
IPR000253. FHA_dom.
IPR001478. PDZ.
IPR000159. Ras-assoc.
IPR008984. SMAD_FHA_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF01843. DIL. 1 hit.
PF00498. FHA. 1 hit.
PF00595. PDZ. 1 hit.
PF00788. RA. 2 hits.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
SM00228. PDZ. 1 hit.
SM00314. RA. 2 hits.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 2 hits.
PROSITEiPS51126. DILUTE. 1 hit.
PS50106. PDZ. 1 hit.
PS50200. RA. 2 hits.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform 4 (identifier: P55196-4) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSAGGRDEER RKLADIIHHW NANRLDLFEI SQPTEDLEFH GVMRFYFQDK     50
AAGNFATKCI RVSSTATTQD VIETLAEKFR PDMRMLSSPK YSLYEVHVSG 100
ERRLDIDEKP LVVQLNWNKD DREGRFVLKN ENDAIPPKKA QSNGPEKQEK 150
EGVIQNFKRT LSKKEKKEKK KREKEALRQA SDKDDRPFQG EDVENSRLAA 200
EVYKDMPETS FTRTISNPEV VMKRRRQQKL EKRMQEFRSS DGRPDSGGTL 250
RIYADSLKPN IPYKTILLST TDPADFAVAE ALEKYGLEKE NPKDYCIARV 300
MLPPGAQHSD EKGAKEIILD DDECPLQIFR EWPSDKGILV FQLKRRPPDH 350
IPKKTKKHLE GKTPKGKERA DGSGYGSTLP PEKLPYLVEL SPGRRNHFAY 400
YNYHTYEDGS DSRDKPKLYR LQLSVTEVGT EKLDDNSIQL FGPGIQPHHC 450
DLTNMDGVVT VTPRSMDAET YVEGQRISET TMLQSGMKVQ FGASHVFKFV 500
DPSQDHALAK RSVDGGLMVK GPRHKPGIVQ ETTFDLGGDI HSGTALPTSK 550
STTRLDSDRV SSASSTAERG MVKPMIRVEQ QPDYRRQESR TQDASGPELI 600
LPASIEFRES SEDSFLSAII NYTNSSTVHF KLSPTYVLYM ACRYVLSNQY 650
RPDISPTERT HKVIAVVNKM VSMMEGVIQK QKNIAGALAF WMANASELLN 700
FIKQDRDLSR ITLDAQDVLA HLVQMAFKYL VHCLQSELNN YMPAFLDDPE 750
ENSLQRPKID DVLHTLTGAM SLLRRCRVNA ALTIQLFSQL FHFINMWLFN 800
RLVTDPDSGL CSHYWGAIIR QQLGHIEAWA EKQGLELAAD CHLSRIVQAT 850
TLLTMDKYAP DDIPNINSTC FKLNSLQLQA LLQNYHCAPD EPFIPTDLIE 900
NVVTVAENTA DELARSDGRE VQLEEDPDLQ LPFLLPEDGY SCDVVRNIPN 950
GLQEFLDPLC QRGFCRLIPH TRSPGTWTIY FEGADYESHL LRENTELAQP 1000
LRKEPEIITV TLKKQNGMGL SIVAAKGAGQ DKLGIYVKSV VKGGAADVDG 1050
RLAAGDQLLS VDGRSLVGLS QERAAELMTR TSSVVTLEVA KQGAIYHGLA 1100
TLLNQPSPMM QRISDRRGSG KPRPKSEGFE LYNNSTQNGS PESPQLPWAE 1150
YSEPKKLPGD DRLMKNRADH RSSPNVANQP PSPGGKSAYA SGTTAKITSV 1200
STGNLCTEEQ TPPPRPEAYP IPTQTYTREY FTFPASKSQD RMAPPQNQWP 1250
NYEEKPHMHT DSNHSSIAIQ RVTRSQEELR EDKAYQLERH RIEAAMDRKS 1300
DSDMWINQSS SLDSSTSSQE HLNHSSKSVT PASTLTKSGP GRWKTPAAIP 1350
ATPVAVSQPI RTDLPPPPPP PPVHYAGDFD GMSMDLPLPP PPSANQIGLP 1400
SAQVAAAERR KREEHQRWYE KEKARLEEER ERKRREQERK LGQMRTQSLN 1450
PAPFSPLTAQ QMKPEKPSTL QRPQETVIRE LQPQQQPRTI ERRDLQYITV 1500
SKEELSSGDS LSPDPWKRDA KEKLEKQQQM HIVDMLSKEI QELQSKPDRS 1550
AEESDRLRKL MLEWQFQKRL QESKQKDEDD EEEEDDDVDT MLIMQRLEAE 1600
RRARLQDEER RRQQQLEEMR KREAEDRARQ EEERRRQEEE RTKRDAEEKR 1650
RQEEGYYSRL EAERRRQHDE AARRLLEPEA PGLCRPPLPR DYEPPSPSPA 1700
PGAPPPPPQR NASYLKTQVL SPDSLFTAKF VAYNEEEEEE DCSLAGPNSY 1750
PGSTGAAVGA HDACRDAKEK RSKSQDADSP GSSGAPENLT FKERQRLFSQ 1800
GQDVSNKVKA SRKLTELENE LNTK 1824
Length:1,824
Mass (Da):206,804
Last modified:February 9, 2010 - v3
Checksum:i51486232F183A3BA
GO
Isoform 1 (identifier: P55196-2) [UniParc]FASTAAdd to Basket

Also known as: s-afadin

The sequence of this isoform differs from the canonical sequence as follows:
     139-139: Missing.
     393-407: Missing.
     1605-1628: LQDEERRRQQQLEEMRKREAEDRA → VKGGVLWLCPSVVPILASACFPWG
     1629-1824: Missing.

Note: May be due to intron retention.

Show »
Length:1,612
Mass (Da):182,000
Checksum:i202D7BEC1E825AB6
GO
Isoform 2 (identifier: P55196-1) [UniParc]FASTAAdd to Basket

Also known as: l-afadin

The sequence of this isoform differs from the canonical sequence as follows:
     139-139: Missing.
     393-407: Missing.
     1048-1048: Missing.
     1747-1824: PNSYPGSTGA...TELENELNTK → QDKYSSTRKS...KRVTNQLSLS

Note: May be due to intron retention.

Show »
Length:1,816
Mass (Da):205,605
Checksum:iEB1FE7F04879CE8F
GO
Isoform 3 (identifier: P55196-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     139-139: Missing.
     393-407: Missing.
     1048-1048: Missing.
     1683-1746: Missing.

Note: No experimental confirmation available.

Show »
Length:1,743
Mass (Da):197,653
Checksum:iECCDE46AD8C401B9
GO
Isoform 6 (identifier: P55196-6) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1605-1606: Missing.
     1650-1653: RRQE → VMVL
     1654-1824: Missing.

Show »
Length:1,651
Mass (Da):187,672
Checksum:i6F55A481FC0DB6B3
GO
Isoform 5 (identifier: P55196-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     139-139: Missing.
     1604-1604: R → RTAMPAISVLDL

Note: No experimental confirmation available.

Show »
Length:1,834
Mass (Da):207,788
Checksum:i3D57FDBD6A521DFD
GO

Sequence cautioni

The sequence CAB82312.1 differs from that shown. Reason: Frameshift at positions 1509, 1512 and 1701.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei139 – 1391Missing in isoform 1, isoform 2, isoform 3 and isoform 5.
VSP_038707
Alternative sequencei393 – 40715Missing in isoform 1, isoform 2 and isoform 3.
VSP_038708Add
BLAST
Alternative sequencei1048 – 10481Missing in isoform 2 and isoform 3.
VSP_038709
Alternative sequencei1604 – 16041R → RTAMPAISVLDL in isoform 5.
VSP_019257
Alternative sequencei1605 – 162824LQDEE…AEDRA → VKGGVLWLCPSVVPILASAC FPWG in isoform 1.
VSP_000217Add
BLAST
Alternative sequencei1605 – 16062Missing in isoform 6.
VSP_041197
Alternative sequencei1629 – 1824196Missing in isoform 1.
VSP_000218Add
BLAST
Alternative sequencei1650 – 16534RRQE → VMVL in isoform 6.
VSP_041198
Alternative sequencei1654 – 1824171Missing in isoform 6.
VSP_041199Add
BLAST
Alternative sequencei1683 – 174664Missing in isoform 3.
VSP_038710Add
BLAST
Alternative sequencei1747 – 182478PNSYP…ELNTK → QDKYSSTRKSHGDLLPAPLK PRPPPCQPRPASDGVFLSNS FQPPSAKANSTAHKKGQPLP PPKKSSSYHPSHCKGRGKRV TNQLSLS in isoform 2.
VSP_038711Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti374 – 3741G → V in AAC50059. 1 Publication
Sequence conflicti1425 – 14251R → P in AAC50059. 1 Publication
Isoform 1 (identifier: P55196-2)
Sequence conflicti1031 – 10322DV → D in AAC50059. 1 Publication
Sequence conflicti1031 – 10322DV → D in BAA32485. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02478 mRNA. Translation: AAC50059.1.
AB011399 Genomic DNA. Translation: BAA32483.1.
AB011399 Genomic DNA. Translation: BAA32484.1.
AB011399 Genomic DNA. Translation: BAA32485.1.
AL009178, AL049698, AL731868 Genomic DNA. Translation: CAI20900.1.
AL009178, AL049698, AL731868 Genomic DNA. Translation: CAI20902.1.
AL731868, AL009178, AL049698 Genomic DNA. Translation: CAI40824.1.
AL731868, AL009178, AL049698 Genomic DNA. Translation: CAI40826.1.
AL049698, AL009178, AL731868 Genomic DNA. Translation: CAI42812.1.
AL049698, AL009178, AL731868 Genomic DNA. Translation: CAI42814.1.
CH471051 Genomic DNA. Translation: EAW47482.1.
AB209420 mRNA. Translation: BAD92657.1.
AL161973 mRNA. Translation: CAB82312.1. Frameshift.
CCDSiCCDS47517.1. [P55196-6]
PIRiT47137.
RefSeqiNP_001035089.1. NM_001040000.2. [P55196-6]
NP_001193937.1. NM_001207008.1. [P55196-3]
XP_005267053.1. XM_005266996.1. [P55196-4]
UniGeneiHs.614974.
Hs.728849.
Hs.741785.

Genome annotation databases

EnsembliENST00000392108; ENSP00000375956; ENSG00000130396. [P55196-6]
ENST00000400822; ENSP00000383623; ENSG00000130396. [P55196-5]
ENST00000447894; ENSP00000404595; ENSG00000130396. [P55196-4]
GeneIDi4301.
KEGGihsa:4301.
UCSCiuc003qwb.1. human. [P55196-2]
uc003qwc.2. human. [P55196-6]
uc003qwg.1. human. [P55196-4]
uc021zij.1. human. [P55196-3]

Polymorphism databases

DMDMi288558835.

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02478 mRNA. Translation: AAC50059.1 .
AB011399 Genomic DNA. Translation: BAA32483.1 .
AB011399 Genomic DNA. Translation: BAA32484.1 .
AB011399 Genomic DNA. Translation: BAA32485.1 .
AL009178 , AL049698 , AL731868 Genomic DNA. Translation: CAI20900.1 .
AL009178 , AL049698 , AL731868 Genomic DNA. Translation: CAI20902.1 .
AL731868 , AL009178 , AL049698 Genomic DNA. Translation: CAI40824.1 .
AL731868 , AL009178 , AL049698 Genomic DNA. Translation: CAI40826.1 .
AL049698 , AL009178 , AL731868 Genomic DNA. Translation: CAI42812.1 .
AL049698 , AL009178 , AL731868 Genomic DNA. Translation: CAI42814.1 .
CH471051 Genomic DNA. Translation: EAW47482.1 .
AB209420 mRNA. Translation: BAD92657.1 .
AL161973 mRNA. Translation: CAB82312.1 . Frameshift.
CCDSi CCDS47517.1. [P55196-6 ]
PIRi T47137.
RefSeqi NP_001035089.1. NM_001040000.2. [P55196-6 ]
NP_001193937.1. NM_001207008.1. [P55196-3 ]
XP_005267053.1. XM_005266996.1. [P55196-4 ]
UniGenei Hs.614974.
Hs.728849.
Hs.741785.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1T2M NMR - A 1003-1094 [» ]
1XZ9 NMR - A 1001-1096 [» ]
2AIN NMR - A 1003-1094 [» ]
2EXG NMR - A 1001-1096 [» ]
ProteinModelPortali P55196.
SMRi P55196. Positions 42-134, 249-348, 374-503, 603-956, 1001-1096, 1521-1687.
ModBasei Search...

Protein-protein interaction databases

BioGridi 110447. 47 interactions.
IntActi P55196. 22 interactions.
MINTi MINT-90828.
STRINGi 9606.ENSP00000383623.

PTM databases

PhosphoSitei P55196.

Polymorphism databases

DMDMi 288558835.

Proteomic databases

MaxQBi P55196.
PaxDbi P55196.
PRIDEi P55196.

Protocols and materials databases

DNASUi 4301.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000392108 ; ENSP00000375956 ; ENSG00000130396 . [P55196-6 ]
ENST00000400822 ; ENSP00000383623 ; ENSG00000130396 . [P55196-5 ]
ENST00000447894 ; ENSP00000404595 ; ENSG00000130396 . [P55196-4 ]
GeneIDi 4301.
KEGGi hsa:4301.
UCSCi uc003qwb.1. human. [P55196-2 ]
uc003qwc.2. human. [P55196-6 ]
uc003qwg.1. human. [P55196-4 ]
uc021zij.1. human. [P55196-3 ]

Organism-specific databases

CTDi 4301.
GeneCardsi GC06P168227.
HGNCi HGNC:7137. MLLT4.
HPAi CAB013496.
HPA030212.
HPA030213.
HPA030214.
HPA030215.
HPA049868.
MIMi 159559. gene.
neXtProti NX_P55196.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG291597.
HOVERGENi HBG050463.
KOi K05702.
OMAi LIPHTRS.
PhylomeDBi P55196.
TreeFami TF350731.

Enzyme and pathway databases

Reactomei REACT_19195. Adherens junctions interactions.
SignaLinki P55196.

Miscellaneous databases

ChiTaRSi MLLT4. human.
EvolutionaryTracei P55196.
GeneWikii MLLT4.
GenomeRNAii 4301.
NextBioi 16931.
PROi P55196.
SOURCEi Search...

Gene expression databases

ArrayExpressi P55196.
Bgeei P55196.
CleanExi HS_MLLT4.
Genevestigatori P55196.

Family and domain databases

Gene3Di 2.30.42.10. 1 hit.
2.60.200.20. 1 hit.
InterProi IPR018444. Dil_domain.
IPR002710. Dilute.
IPR000253. FHA_dom.
IPR001478. PDZ.
IPR000159. Ras-assoc.
IPR008984. SMAD_FHA_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF01843. DIL. 1 hit.
PF00498. FHA. 1 hit.
PF00595. PDZ. 1 hit.
PF00788. RA. 2 hits.
[Graphical view ]
SMARTi SM00240. FHA. 1 hit.
SM00228. PDZ. 1 hit.
SM00314. RA. 2 hits.
[Graphical view ]
SUPFAMi SSF49879. SSF49879. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 2 hits.
PROSITEi PS51126. DILUTE. 1 hit.
PS50106. PDZ. 1 hit.
PS50200. RA. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in acute myeloid leukemias with the t(6;11) chromosome translocation."
    Prasad R., Gu Y., Alder H., Nakamura T., Canaani O., Saito H., Huebner K., Gale R.P., Nowell P.C., Kuriyama K., Miyazaki Y., Croce C.M., Canaani E.
    Cancer Res. 53:5624-5628(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHROMOSOMAL TRANSLOCATION WITH KMT2A.
  2. "Complete genomic structure, DNA polymorphisms, and alternative splicing of the human AF-6 gene."
    Saito S., Matsushima M., Shirahama S., Minaguchi T., Kanamori Y., Minami M., Nakamura Y.
    DNA Res. 5:115-120(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1; 2 AND 3).
    Tissue: Fetal brain.
  3. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Homo sapiens protein coding cDNA."
    Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 13-1824 (ISOFORM 6).
    Tissue: Brain.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 335-1824 (ISOFORM 5).
    Tissue: Amygdala.
  7. "Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin."
    Reymond N., Borg J.-P., Lecocq E., Adelaide J., Campadelli-Fiume G., Dubreuil P., Lopez M.
    Gene 255:347-355(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PVRL3.
  8. "Nectin and afadin: novel organizers of intercellular junctions."
    Takai Y., Nakanishi H.
    J. Cell Sci. 116:17-27(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON INTERACTION.
  9. "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
    Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.
    Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1182, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  10. "Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction."
    Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S., Li S.-J., Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R.
    Mol. Cell. Proteomics 6:1952-1967(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1182, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  11. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216; SER-1107; SER-1182; THR-1232; SER-1721; SER-1779 AND SER-1799, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  13. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1182 AND SER-1721, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  15. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1721; SER-1779 AND SER-1799, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  16. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "Solution structure of AF-6 PDZ domain and its interaction with the C-terminal peptides from Neurexin and Bcr."
    Zhou H., Xu Y., Yang Y., Huang A., Wu J., Shi Y.
    J. Biol. Chem. 280:13841-13847(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1003-1094 IN COMPLEX WITH NRXN1 AND BCR.
  18. Cited for: STRUCTURE BY NMR OF 1001-1095.

Entry informationi

Entry nameiAFAD_HUMAN
AccessioniPrimary (citable) accession number: P55196
Secondary accession number(s): O75087
, O75088, O75089, Q59FP0, Q5TIG6, Q5TIG7, Q9NSN7, Q9NU92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: February 9, 2010
Last modified: September 3, 2014
This is version 157 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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