P55196 (AFAD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Afadin

Alternative name(s):
ALL1-fused gene from chromosome 6 protein
Short name=Protein AF-6

Gene names

Name:	MLLT4
Synonyms:	AF6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1824 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
Subunit structure	Homodimer. Interacts with F-actin, nectin and PVRL3/nectin-3. Essential for the association of nectin and E-cadherin. Isoform 1/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1 and RIT2 By similarity. Interacts with NRXN1 and BCR. Ref.7
Subcellular location	Cell junction › adherens junction. Note: Not found at cell-matrix AJs.
Domain	The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich N-terminus domain interacts with F-actin.
Involvement in disease	Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.
Sequence similarities	Contains 1 dilute domain. Contains 1 FHA domain. Contains 1 PDZ (DHR) domain. Contains 2 Ras-associating domains.
Sequence caution	The sequence CAB82312.1 differs from that shown. Reason: Frameshift at positions 1509, 1512 and 1701.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell junction
Coding sequence diversity	Alternative splicing Chromosomal rearrangement
Disease	Proto-oncogene
Domain	Coiled coil Repeat
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	adherens junction organization Traceable author statement. Source: Reactome cell adhesion Traceable author statement. Source: ProtInc cell junction assembly Traceable author statement. Source: Reactome cell-cell signaling Traceable author statement. Source: ProtInc signal transduction Traceable author statement. Source: ProtInc
Cellular component	adherens junction Inferred from electronic annotation. Source: UniProtKB-SubCell cell-cell junction Traceable author statement. Source: ProtInc cytosol Traceable author statement. Source: Reactome nucleus Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Molecular function	protein C-terminus binding Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 4 (identifier: P55196-4) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: P55196-2) Also known as: s-afadin; The sequence of this isoform differs from the canonical sequence as follows:      139-139: Missing.      393-407: Missing.      1605-1628: LQDEERRRQQQLEEMRKREAEDRA → VKGGVLWLCPSVVPILASACFPWG      1629-1824: Missing.
Note: May be due to intron retention.
Sequence annotation (Features)
	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
	Sequence conflict	1031 – 1032	2	DV → D in AAC50059. Ref.1
	Sequence conflict	1031 – 1032	2	DV → D in BAA32485. Ref.2
Isoform 2 (identifier: P55196-1) Also known as: l-afadin; The sequence of this isoform differs from the canonical sequence as follows:      139-139: Missing.      393-407: Missing.      1048-1048: Missing.      1747-1824: PNSYPGSTGA...TELENELNTK → QDKYSSTRKS...KRVTNQLSLS
Note: May be due to intron retention.
Isoform 3 (identifier: P55196-3) The sequence of this isoform differs from the canonical sequence as follows:      139-139: Missing.      393-407: Missing.      1048-1048: Missing.      1683-1746: Missing.
Note: No experimental confirmation available.
Isoform 6 (identifier: P55196-6) The sequence of this isoform differs from the canonical sequence as follows:      1605-1606: Missing.      1650-1653: RRQE → VMVL      1654-1824: Missing.
Isoform 5 (identifier: P55196-5) The sequence of this isoform differs from the canonical sequence as follows:      139-139: Missing.      1604-1604: R → RTAMPAISVLDL
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1824

1824

Afadin

PRO_0000215918

Regions

Domain

39 – 133

95

Ras-associating 1

Domain

246 – 348

103

Ras-associating 2

Domain

426 – 492

67

FHA

Domain

668 – 908

241

Dilute

Domain

1007 – 1093

87

PDZ

Coiled coil

146 – 185

40

Potential

Coiled coil

1408 – 1448

41

Potential

Coiled coil

1523 – 1667

145

Potential

Compositional bias

163 – 175

13

Glu/Lys-rich

Compositional bias

1346 – 1392

47

Pro-rich

Compositional bias

1578 – 1588

11

Asp/Glu-rich (acidic)

Compositional bias

1678 – 1708

31

Pro-rich

Sites

Site

26

1

Breakpoint for translocation to form MLL-MLLT4

Amino acid modifications

Modified residue

94

1

Phosphotyrosine Ref.13

Modified residue

216

1

Phosphoserine Ref.17 Ref.18 Ref.19

Modified residue

557

1

Phosphoserine By similarity

Modified residue

1083

1

Phosphoserine Ref.19

Modified residue

1107

1

Phosphoserine Ref.11 Ref.17

Modified residue

1172

1

Phosphoserine Ref.9 Ref.18

Modified residue

1173

1

Phosphoserine Ref.9 Ref.18

Modified residue

1182

1

Phosphoserine Ref.10 Ref.14 Ref.17 Ref.18 Ref.19

Modified residue

1201

1

Phosphoserine Ref.10

Modified residue

1230

1

Phosphotyrosine Ref.15

Modified residue

1232

1

Phosphothreonine Ref.17

Modified residue

1275

1

Phosphoserine By similarity

Modified residue

1330

1

Phosphothreonine Ref.18

Modified residue

1334

1

Phosphothreonine By similarity

Modified residue

1512

1

Phosphoserine Ref.18

Modified residue

1696

1

Phosphoserine Ref.16

Modified residue

1698

1

Phosphoserine Ref.12

Modified residue

1721

1

Phosphoserine Ref.17 Ref.18 Ref.19

Natural variations

Alternative sequence

139

1

Missing in isoform 1, isoform 2, isoform 3 and isoform 5.

VSP_038707

Alternative sequence

393 – 407

15

Missing in isoform 1, isoform 2 and isoform 3.

VSP_038708

Alternative sequence

1048

1

Missing in isoform 2 and isoform 3.

VSP_038709

Alternative sequence

1604

1

R → RTAMPAISVLDL in isoform 5.

VSP_019257

Alternative sequence

1605 – 1628

24

LQDEE…AEDRA → VKGGVLWLCPSVVPILASAC FPWG in isoform 1.

VSP_000217

Alternative sequence

1605 – 1606

2

Missing in isoform 6.

VSP_041197

Alternative sequence

1629 – 1824

196

Missing in isoform 1.

VSP_000218

Alternative sequence

1650 – 1653

4

RRQE → VMVL in isoform 6.

VSP_041198

Alternative sequence

1654 – 1824

171

Missing in isoform 6.

VSP_041199

Alternative sequence

1683 – 1746

64

Missing in isoform 3.

VSP_038710

Alternative sequence

1747 – 1824

78

PNSYP…ELNTK → QDKYSSTRKSHGDLLPAPLK PRPPPCQPRPASDGVFLSNS FQPPSAKANSTAHKKGQPLP PPKKSSSYHPSHCKGRGKRV TNQLSLS in isoform 2.

VSP_038711

Experimental info

Sequence conflict

374

1

G → V in AAC50059. Ref.1

Sequence conflict

1425

1

R → P in AAC50059. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1824

Helix Strand Turn

Details...

Beta strand

1006 – 1013

8

Beta strand

1015 – 1017

3

Beta strand

1020 – 1025

6

Beta strand

1034 – 1040

7

Helix

1045 – 1049

5

Beta strand

1054 – 1061

8

Helix

1071 – 1079

9

Beta strand

1083 – 1091

9

Sequences

	Sequence		Length	Mass (Da)
	Isoform 4 [UniParc]. Last modified February 9, 2010. Version 3. Checksum: 51486232F183A3BA	FASTA	1,824	206,804
	10 20 30 40 50 60 MSAGGRDEER RKLADIIHHW NANRLDLFEI SQPTEDLEFH GVMRFYFQDK AAGNFATKCI 70 80 90 100 110 120 RVSSTATTQD VIETLAEKFR PDMRMLSSPK YSLYEVHVSG ERRLDIDEKP LVVQLNWNKD 130 140 150 160 170 180 DREGRFVLKN ENDAIPPKKA QSNGPEKQEK EGVIQNFKRT LSKKEKKEKK KREKEALRQA 190 200 210 220 230 240 SDKDDRPFQG EDVENSRLAA EVYKDMPETS FTRTISNPEV VMKRRRQQKL EKRMQEFRSS 250 260 270 280 290 300 DGRPDSGGTL RIYADSLKPN IPYKTILLST TDPADFAVAE ALEKYGLEKE NPKDYCIARV 310 320 330 340 350 360 MLPPGAQHSD EKGAKEIILD DDECPLQIFR EWPSDKGILV FQLKRRPPDH IPKKTKKHLE 370 380 390 400 410 420 GKTPKGKERA DGSGYGSTLP PEKLPYLVEL SPGRRNHFAY YNYHTYEDGS DSRDKPKLYR 430 440 450 460 470 480 LQLSVTEVGT EKLDDNSIQL FGPGIQPHHC DLTNMDGVVT VTPRSMDAET YVEGQRISET 490 500 510 520 530 540 TMLQSGMKVQ FGASHVFKFV DPSQDHALAK RSVDGGLMVK GPRHKPGIVQ ETTFDLGGDI 550 560 570 580 590 600 HSGTALPTSK STTRLDSDRV SSASSTAERG MVKPMIRVEQ QPDYRRQESR TQDASGPELI 610 620 630 640 650 660 LPASIEFRES SEDSFLSAII NYTNSSTVHF KLSPTYVLYM ACRYVLSNQY RPDISPTERT 670 680 690 700 710 720 HKVIAVVNKM VSMMEGVIQK QKNIAGALAF WMANASELLN FIKQDRDLSR ITLDAQDVLA 730 740 750 760 770 780 HLVQMAFKYL VHCLQSELNN YMPAFLDDPE ENSLQRPKID DVLHTLTGAM SLLRRCRVNA 790 800 810 820 830 840 ALTIQLFSQL FHFINMWLFN RLVTDPDSGL CSHYWGAIIR QQLGHIEAWA EKQGLELAAD 850 860 870 880 890 900 CHLSRIVQAT TLLTMDKYAP DDIPNINSTC FKLNSLQLQA LLQNYHCAPD EPFIPTDLIE 910 920 930 940 950 960 NVVTVAENTA DELARSDGRE VQLEEDPDLQ LPFLLPEDGY SCDVVRNIPN GLQEFLDPLC 970 980 990 1000 1010 1020 QRGFCRLIPH TRSPGTWTIY FEGADYESHL LRENTELAQP LRKEPEIITV TLKKQNGMGL 1030 1040 1050 1060 1070 1080 SIVAAKGAGQ DKLGIYVKSV VKGGAADVDG RLAAGDQLLS VDGRSLVGLS QERAAELMTR 1090 1100 1110 1120 1130 1140 TSSVVTLEVA KQGAIYHGLA TLLNQPSPMM QRISDRRGSG KPRPKSEGFE LYNNSTQNGS 1150 1160 1170 1180 1190 1200 PESPQLPWAE YSEPKKLPGD DRLMKNRADH RSSPNVANQP PSPGGKSAYA SGTTAKITSV 1210 1220 1230 1240 1250 1260 STGNLCTEEQ TPPPRPEAYP IPTQTYTREY FTFPASKSQD RMAPPQNQWP NYEEKPHMHT 1270 1280 1290 1300 1310 1320 DSNHSSIAIQ RVTRSQEELR EDKAYQLERH RIEAAMDRKS DSDMWINQSS SLDSSTSSQE 1330 1340 1350 1360 1370 1380 HLNHSSKSVT PASTLTKSGP GRWKTPAAIP ATPVAVSQPI RTDLPPPPPP PPVHYAGDFD 1390 1400 1410 1420 1430 1440 GMSMDLPLPP PPSANQIGLP SAQVAAAERR KREEHQRWYE KEKARLEEER ERKRREQERK 1450 1460 1470 1480 1490 1500 LGQMRTQSLN PAPFSPLTAQ QMKPEKPSTL QRPQETVIRE LQPQQQPRTI ERRDLQYITV 1510 1520 1530 1540 1550 1560 SKEELSSGDS LSPDPWKRDA KEKLEKQQQM HIVDMLSKEI QELQSKPDRS AEESDRLRKL 1570 1580 1590 1600 1610 1620 MLEWQFQKRL QESKQKDEDD EEEEDDDVDT MLIMQRLEAE RRARLQDEER RRQQQLEEMR 1630 1640 1650 1660 1670 1680 KREAEDRARQ EEERRRQEEE RTKRDAEEKR RQEEGYYSRL EAERRRQHDE AARRLLEPEA 1690 1700 1710 1720 1730 1740 PGLCRPPLPR DYEPPSPSPA PGAPPPPPQR NASYLKTQVL SPDSLFTAKF VAYNEEEEEE 1750 1760 1770 1780 1790 1800 DCSLAGPNSY PGSTGAAVGA HDACRDAKEK RSKSQDADSP GSSGAPENLT FKERQRLFSQ 1810 1820 GQDVSNKVKA SRKLTELENE LNTK
	Isoform 1 (s-afadin) [UniParc]. Checksum: 202D7BEC1E825AB6	FASTA	1,612	182,000
	10 20 30 40 50 60 MSAGGRDEER RKLADIIHHW NANRLDLFEI SQPTEDLEFH GVMRFYFQDK AAGNFATKCI 70 80 90 100 110 120 RVSSTATTQD VIETLAEKFR PDMRMLSSPK YSLYEVHVSG ERRLDIDEKP LVVQLNWNKD 130 140 150 160 170 180 DREGRFVLKN ENDAIPPKAQ SNGPEKQEKE GVIQNFKRTL SKKEKKEKKK REKEALRQAS 190 200 210 220 230 240 DKDDRPFQGE DVENSRLAAE VYKDMPETSF TRTISNPEVV MKRRRQQKLE KRMQEFRSSD 250 260 270 280 290 300 GRPDSGGTLR IYADSLKPNI PYKTILLSTT DPADFAVAEA LEKYGLEKEN PKDYCIARVM 310 320 330 340 350 360 LPPGAQHSDE KGAKEIILDD DECPLQIFRE WPSDKGILVF QLKRRPPDHI PKKTKKHLEG 370 380 390 400 410 420 KTPKGKERAD GSGYGSTLPP EKLPYLVELS PDGSDSRDKP KLYRLQLSVT EVGTEKLDDN 430 440 450 460 470 480 SIQLFGPGIQ PHHCDLTNMD GVVTVTPRSM DAETYVEGQR ISETTMLQSG MKVQFGASHV 490 500 510 520 530 540 FKFVDPSQDH ALAKRSVDGG LMVKGPRHKP GIVQETTFDL GGDIHSGTAL PTSKSTTRLD 550 560 570 580 590 600 SDRVSSASST AERGMVKPMI RVEQQPDYRR QESRTQDASG PELILPASIE FRESSEDSFL 610 620 630 640 650 660 SAIINYTNSS TVHFKLSPTY VLYMACRYVL SNQYRPDISP TERTHKVIAV VNKMVSMMEG 670 680 690 700 710 720 VIQKQKNIAG ALAFWMANAS ELLNFIKQDR DLSRITLDAQ DVLAHLVQMA FKYLVHCLQS 730 740 750 760 770 780 ELNNYMPAFL DDPEENSLQR PKIDDVLHTL TGAMSLLRRC RVNAALTIQL FSQLFHFINM 790 800 810 820 830 840 WLFNRLVTDP DSGLCSHYWG AIIRQQLGHI EAWAEKQGLE LAADCHLSRI VQATTLLTMD 850 860 870 880 890 900 KYAPDDIPNI NSTCFKLNSL QLQALLQNYH CAPDEPFIPT DLIENVVTVA ENTADELARS 910 920 930 940 950 960 DGREVQLEED PDLQLPFLLP EDGYSCDVVR NIPNGLQEFL DPLCQRGFCR LIPHTRSPGT 970 980 990 1000 1010 1020 WTIYFEGADY ESHLLRENTE LAQPLRKEPE IITVTLKKQN GMGLSIVAAK GAGQDKLGIY 1030 1040 1050 1060 1070 1080 VKSVVKGGAA DVDGRLAAGD QLLSVDGRSL VGLSQERAAE LMTRTSSVVT LEVAKQGAIY 1090 1100 1110 1120 1130 1140 HGLATLLNQP SPMMQRISDR RGSGKPRPKS EGFELYNNST QNGSPESPQL PWAEYSEPKK 1150 1160 1170 1180 1190 1200 LPGDDRLMKN RADHRSSPNV ANQPPSPGGK SAYASGTTAK ITSVSTGNLC TEEQTPPPRP 1210 1220 1230 1240 1250 1260 EAYPIPTQTY TREYFTFPAS KSQDRMAPPQ NQWPNYEEKP HMHTDSNHSS IAIQRVTRSQ 1270 1280 1290 1300 1310 1320 EELREDKAYQ LERHRIEAAM DRKSDSDMWI NQSSSLDSST SSQEHLNHSS KSVTPASTLT 1330 1340 1350 1360 1370 1380 KSGPGRWKTP AAIPATPVAV SQPIRTDLPP PPPPPPVHYA GDFDGMSMDL PLPPPPSANQ 1390 1400 1410 1420 1430 1440 IGLPSAQVAA AERRKREEHQ RWYEKEKARL EEERERKRRE QERKLGQMRT QSLNPAPFSP 1450 1460 1470 1480 1490 1500 LTAQQMKPEK PSTLQRPQET VIRELQPQQQ PRTIERRDLQ YITVSKEELS SGDSLSPDPW 1510 1520 1530 1540 1550 1560 KRDAKEKLEK QQQMHIVDML SKEIQELQSK PDRSAEESDR LRKLMLEWQF QKRLQESKQK 1570 1580 1590 1600 1610 DEDDEEEEDD DVDTMLIMQR LEAERRARVK GGVLWLCPSV VPILASACFP WG
	Isoform 2 (l-afadin) [UniParc]. Checksum: EB1FE7F04879CE8F	FASTA	1,816	205,605
	10 20 30 40 50 60 MSAGGRDEER RKLADIIHHW NANRLDLFEI SQPTEDLEFH GVMRFYFQDK AAGNFATKCI 70 80 90 100 110 120 RVSSTATTQD VIETLAEKFR PDMRMLSSPK YSLYEVHVSG ERRLDIDEKP LVVQLNWNKD 130 140 150 160 170 180 DREGRFVLKN ENDAIPPKAQ SNGPEKQEKE GVIQNFKRTL SKKEKKEKKK REKEALRQAS 190 200 210 220 230 240 DKDDRPFQGE DVENSRLAAE VYKDMPETSF TRTISNPEVV MKRRRQQKLE KRMQEFRSSD 250 260 270 280 290 300 GRPDSGGTLR IYADSLKPNI PYKTILLSTT DPADFAVAEA LEKYGLEKEN PKDYCIARVM 310 320 330 340 350 360 LPPGAQHSDE KGAKEIILDD DECPLQIFRE WPSDKGILVF QLKRRPPDHI PKKTKKHLEG 370 380 390 400 410 420 KTPKGKERAD GSGYGSTLPP EKLPYLVELS PDGSDSRDKP KLYRLQLSVT EVGTEKLDDN 430 440 450 460 470 480 SIQLFGPGIQ PHHCDLTNMD GVVTVTPRSM DAETYVEGQR ISETTMLQSG MKVQFGASHV 490 500 510 520 530 540 FKFVDPSQDH ALAKRSVDGG LMVKGPRHKP GIVQETTFDL GGDIHSGTAL PTSKSTTRLD 550 560 570 580 590 600 SDRVSSASST AERGMVKPMI RVEQQPDYRR QESRTQDASG PELILPASIE FRESSEDSFL 610 620 630 640 650 660 SAIINYTNSS TVHFKLSPTY VLYMACRYVL SNQYRPDISP TERTHKVIAV VNKMVSMMEG 670 680 690 700 710 720 VIQKQKNIAG ALAFWMANAS ELLNFIKQDR DLSRITLDAQ DVLAHLVQMA FKYLVHCLQS 730 740 750 760 770 780 ELNNYMPAFL DDPEENSLQR PKIDDVLHTL TGAMSLLRRC RVNAALTIQL FSQLFHFINM 790 800 810 820 830 840 WLFNRLVTDP DSGLCSHYWG AIIRQQLGHI EAWAEKQGLE LAADCHLSRI VQATTLLTMD 850 860 870 880 890 900 KYAPDDIPNI NSTCFKLNSL QLQALLQNYH CAPDEPFIPT DLIENVVTVA ENTADELARS 910 920 930 940 950 960 DGREVQLEED PDLQLPFLLP EDGYSCDVVR NIPNGLQEFL DPLCQRGFCR LIPHTRSPGT 970 980 990 1000 1010 1020 WTIYFEGADY ESHLLRENTE LAQPLRKEPE IITVTLKKQN GMGLSIVAAK GAGQDKLGIY 1030 1040 1050 1060 1070 1080 VKSVVKGGAA DDGRLAAGDQ LLSVDGRSLV GLSQERAAEL MTRTSSVVTL EVAKQGAIYH 1090 1100 1110 1120 1130 1140 GLATLLNQPS PMMQRISDRR GSGKPRPKSE GFELYNNSTQ NGSPESPQLP WAEYSEPKKL 1150 1160 1170 1180 1190 1200 PGDDRLMKNR ADHRSSPNVA NQPPSPGGKS AYASGTTAKI TSVSTGNLCT EEQTPPPRPE 1210 1220 1230 1240 1250 1260 AYPIPTQTYT REYFTFPASK SQDRMAPPQN QWPNYEEKPH MHTDSNHSSI AIQRVTRSQE 1270 1280 1290 1300 1310 1320 ELREDKAYQL ERHRIEAAMD RKSDSDMWIN QSSSLDSSTS SQEHLNHSSK SVTPASTLTK 1330 1340 1350 1360 1370 1380 SGPGRWKTPA AIPATPVAVS QPIRTDLPPP PPPPPVHYAG DFDGMSMDLP LPPPPSANQI 1390 1400 1410 1420 1430 1440 GLPSAQVAAA ERRKREEHQR WYEKEKARLE EERERKRREQ ERKLGQMRTQ SLNPAPFSPL 1450 1460 1470 1480 1490 1500 TAQQMKPEKP STLQRPQETV IRELQPQQQP RTIERRDLQY ITVSKEELSS GDSLSPDPWK 1510 1520 1530 1540 1550 1560 RDAKEKLEKQ QQMHIVDMLS KEIQELQSKP DRSAEESDRL RKLMLEWQFQ KRLQESKQKD 1570 1580 1590 1600 1610 1620 EDDEEEEDDD VDTMLIMQRL EAERRARLQD EERRRQQQLE EMRKREAEDR ARQEEERRRQ 1630 1640 1650 1660 1670 1680 EEERTKRDAE EKRRQEEGYY SRLEAERRRQ HDEAARRLLE PEAPGLCRPP LPRDYEPPSP 1690 1700 1710 1720 1730 1740 SPAPGAPPPP PQRNASYLKT QVLSPDSLFT AKFVAYNEEE EEEDCSLAGQ DKYSSTRKSH 1750 1760 1770 1780 1790 1800 GDLLPAPLKP RPPPCQPRPA SDGVFLSNSF QPPSAKANST AHKKGQPLPP PKKSSSYHPS 1810 HCKGRGKRVT NQLSLS
	Isoform 3 [UniParc]. Checksum: ECCDE46AD8C401B9	FASTA	1,743	197,653
	10 20 30 40 50 60 MSAGGRDEER RKLADIIHHW NANRLDLFEI SQPTEDLEFH GVMRFYFQDK AAGNFATKCI 70 80 90 100 110 120 RVSSTATTQD VIETLAEKFR PDMRMLSSPK YSLYEVHVSG ERRLDIDEKP LVVQLNWNKD 130 140 150 160 170 180 DREGRFVLKN ENDAIPPKAQ SNGPEKQEKE GVIQNFKRTL SKKEKKEKKK REKEALRQAS 190 200 210 220 230 240 DKDDRPFQGE DVENSRLAAE VYKDMPETSF TRTISNPEVV MKRRRQQKLE KRMQEFRSSD 250 260 270 280 290 300 GRPDSGGTLR IYADSLKPNI PYKTILLSTT DPADFAVAEA LEKYGLEKEN PKDYCIARVM 310 320 330 340 350 360 LPPGAQHSDE KGAKEIILDD DECPLQIFRE WPSDKGILVF QLKRRPPDHI PKKTKKHLEG 370 380 390 400 410 420 KTPKGKERAD GSGYGSTLPP EKLPYLVELS PDGSDSRDKP KLYRLQLSVT EVGTEKLDDN 430 440 450 460 470 480 SIQLFGPGIQ PHHCDLTNMD GVVTVTPRSM DAETYVEGQR ISETTMLQSG MKVQFGASHV 490 500 510 520 530 540 FKFVDPSQDH ALAKRSVDGG LMVKGPRHKP GIVQETTFDL GGDIHSGTAL PTSKSTTRLD 550 560 570 580 590 600 SDRVSSASST AERGMVKPMI RVEQQPDYRR QESRTQDASG PELILPASIE FRESSEDSFL 610 620 630 640 650 660 SAIINYTNSS TVHFKLSPTY VLYMACRYVL SNQYRPDISP TERTHKVIAV VNKMVSMMEG 670 680 690 700 710 720 VIQKQKNIAG ALAFWMANAS ELLNFIKQDR DLSRITLDAQ DVLAHLVQMA FKYLVHCLQS 730 740 750 760 770 780 ELNNYMPAFL DDPEENSLQR PKIDDVLHTL TGAMSLLRRC RVNAALTIQL FSQLFHFINM 790 800 810 820 830 840 WLFNRLVTDP DSGLCSHYWG AIIRQQLGHI EAWAEKQGLE LAADCHLSRI VQATTLLTMD 850 860 870 880 890 900 KYAPDDIPNI NSTCFKLNSL QLQALLQNYH CAPDEPFIPT DLIENVVTVA ENTADELARS 910 920 930 940 950 960 DGREVQLEED PDLQLPFLLP EDGYSCDVVR NIPNGLQEFL DPLCQRGFCR LIPHTRSPGT 970 980 990 1000 1010 1020 WTIYFEGADY ESHLLRENTE LAQPLRKEPE IITVTLKKQN GMGLSIVAAK GAGQDKLGIY 1030 1040 1050 1060 1070 1080 VKSVVKGGAA DDGRLAAGDQ LLSVDGRSLV GLSQERAAEL MTRTSSVVTL EVAKQGAIYH 1090 1100 1110 1120 1130 1140 GLATLLNQPS PMMQRISDRR GSGKPRPKSE GFELYNNSTQ NGSPESPQLP WAEYSEPKKL 1150 1160 1170 1180 1190 1200 PGDDRLMKNR ADHRSSPNVA NQPPSPGGKS AYASGTTAKI TSVSTGNLCT EEQTPPPRPE 1210 1220 1230 1240 1250 1260 AYPIPTQTYT REYFTFPASK SQDRMAPPQN QWPNYEEKPH MHTDSNHSSI AIQRVTRSQE 1270 1280 1290 1300 1310 1320 ELREDKAYQL ERHRIEAAMD RKSDSDMWIN QSSSLDSSTS SQEHLNHSSK SVTPASTLTK 1330 1340 1350 1360 1370 1380 SGPGRWKTPA AIPATPVAVS QPIRTDLPPP PPPPPVHYAG DFDGMSMDLP LPPPPSANQI 1390 1400 1410 1420 1430 1440 GLPSAQVAAA ERRKREEHQR WYEKEKARLE EERERKRREQ ERKLGQMRTQ SLNPAPFSPL 1450 1460 1470 1480 1490 1500 TAQQMKPEKP STLQRPQETV IRELQPQQQP RTIERRDLQY ITVSKEELSS GDSLSPDPWK 1510 1520 1530 1540 1550 1560 RDAKEKLEKQ QQMHIVDMLS KEIQELQSKP DRSAEESDRL RKLMLEWQFQ KRLQESKQKD 1570 1580 1590 1600 1610 1620 EDDEEEEDDD VDTMLIMQRL EAERRARLQD EERRRQQQLE EMRKREAEDR ARQEEERRRQ 1630 1640 1650 1660 1670 1680 EEERTKRDAE EKRRQEEGYY SRLEAERRRQ HDEAARRLLE PEAPGPNSYP GSTGAAVGAH 1690 1700 1710 1720 1730 1740 DACRDAKEKR SKSQDADSPG SSGAPENLTF KERQRLFSQG QDVSNKVKAS RKLTELENEL NTK
	Isoform 6 [UniParc]. Checksum: 6F55A481FC0DB6B3	FASTA	1,651	187,672
	10 20 30 40 50 60 MSAGGRDEER RKLADIIHHW NANRLDLFEI SQPTEDLEFH GVMRFYFQDK AAGNFATKCI 70 80 90 100 110 120 RVSSTATTQD VIETLAEKFR PDMRMLSSPK YSLYEVHVSG ERRLDIDEKP LVVQLNWNKD 130 140 150 160 170 180 DREGRFVLKN ENDAIPPKKA QSNGPEKQEK EGVIQNFKRT LSKKEKKEKK KREKEALRQA 190 200 210 220 230 240 SDKDDRPFQG EDVENSRLAA EVYKDMPETS FTRTISNPEV VMKRRRQQKL EKRMQEFRSS 250 260 270 280 290 300 DGRPDSGGTL RIYADSLKPN IPYKTILLST TDPADFAVAE ALEKYGLEKE NPKDYCIARV 310 320 330 340 350 360 MLPPGAQHSD EKGAKEIILD DDECPLQIFR EWPSDKGILV FQLKRRPPDH IPKKTKKHLE 370 380 390 400 410 420 GKTPKGKERA DGSGYGSTLP PEKLPYLVEL SPGRRNHFAY YNYHTYEDGS DSRDKPKLYR 430 440 450 460 470 480 LQLSVTEVGT EKLDDNSIQL FGPGIQPHHC DLTNMDGVVT VTPRSMDAET YVEGQRISET 490 500 510 520 530 540 TMLQSGMKVQ FGASHVFKFV DPSQDHALAK RSVDGGLMVK GPRHKPGIVQ ETTFDLGGDI 550 560 570 580 590 600 HSGTALPTSK STTRLDSDRV SSASSTAERG MVKPMIRVEQ QPDYRRQESR TQDASGPELI 610 620 630 640 650 660 LPASIEFRES SEDSFLSAII NYTNSSTVHF KLSPTYVLYM ACRYVLSNQY RPDISPTERT 670 680 690 700 710 720 HKVIAVVNKM VSMMEGVIQK QKNIAGALAF WMANASELLN FIKQDRDLSR ITLDAQDVLA 730 740 750 760 770 780 HLVQMAFKYL VHCLQSELNN YMPAFLDDPE ENSLQRPKID DVLHTLTGAM SLLRRCRVNA 790 800 810 820 830 840 ALTIQLFSQL FHFINMWLFN RLVTDPDSGL CSHYWGAIIR QQLGHIEAWA EKQGLELAAD 850 860 870 880 890 900 CHLSRIVQAT TLLTMDKYAP DDIPNINSTC FKLNSLQLQA LLQNYHCAPD EPFIPTDLIE 910 920 930 940 950 960 NVVTVAENTA DELARSDGRE VQLEEDPDLQ LPFLLPEDGY SCDVVRNIPN GLQEFLDPLC 970 980 990 1000 1010 1020 QRGFCRLIPH TRSPGTWTIY FEGADYESHL LRENTELAQP LRKEPEIITV TLKKQNGMGL 1030 1040 1050 1060 1070 1080 SIVAAKGAGQ DKLGIYVKSV VKGGAADVDG RLAAGDQLLS VDGRSLVGLS QERAAELMTR 1090 1100 1110 1120 1130 1140 TSSVVTLEVA KQGAIYHGLA TLLNQPSPMM QRISDRRGSG KPRPKSEGFE LYNNSTQNGS 1150 1160 1170 1180 1190 1200 PESPQLPWAE YSEPKKLPGD DRLMKNRADH RSSPNVANQP PSPGGKSAYA SGTTAKITSV 1210 1220 1230 1240 1250 1260 STGNLCTEEQ TPPPRPEAYP IPTQTYTREY FTFPASKSQD RMAPPQNQWP NYEEKPHMHT 1270 1280 1290 1300 1310 1320 DSNHSSIAIQ RVTRSQEELR EDKAYQLERH RIEAAMDRKS DSDMWINQSS SLDSSTSSQE 1330 1340 1350 1360 1370 1380 HLNHSSKSVT PASTLTKSGP GRWKTPAAIP ATPVAVSQPI RTDLPPPPPP PPVHYAGDFD 1390 1400 1410 1420 1430 1440 GMSMDLPLPP PPSANQIGLP SAQVAAAERR KREEHQRWYE KEKARLEEER ERKRREQERK 1450 1460 1470 1480 1490 1500 LGQMRTQSLN PAPFSPLTAQ QMKPEKPSTL QRPQETVIRE LQPQQQPRTI ERRDLQYITV 1510 1520 1530 1540 1550 1560 SKEELSSGDS LSPDPWKRDA KEKLEKQQQM HIVDMLSKEI QELQSKPDRS AEESDRLRKL 1570 1580 1590 1600 1610 1620 MLEWQFQKRL QESKQKDEDD EEEEDDDVDT MLIMQRLEAE RRARDEERRR QQQLEEMRKR 1630 1640 1650 EAEDRARQEE ERRRQEEERT KRDAEEKVMV L
	Isoform 5 [UniParc]. Checksum: 3D57FDBD6A521DFD	FASTA	1,834	207,788
	10 20 30 40 50 60 MSAGGRDEER RKLADIIHHW NANRLDLFEI SQPTEDLEFH GVMRFYFQDK AAGNFATKCI 70 80 90 100 110 120 RVSSTATTQD VIETLAEKFR PDMRMLSSPK YSLYEVHVSG ERRLDIDEKP LVVQLNWNKD 130 140 150 160 170 180 DREGRFVLKN ENDAIPPKAQ SNGPEKQEKE GVIQNFKRTL SKKEKKEKKK REKEALRQAS 190 200 210 220 230 240 DKDDRPFQGE DVENSRLAAE VYKDMPETSF TRTISNPEVV MKRRRQQKLE KRMQEFRSSD 250 260 270 280 290 300 GRPDSGGTLR IYADSLKPNI PYKTILLSTT DPADFAVAEA LEKYGLEKEN PKDYCIARVM 310 320 330 340 350 360 LPPGAQHSDE KGAKEIILDD DECPLQIFRE WPSDKGILVF QLKRRPPDHI PKKTKKHLEG 370 380 390 400 410 420 KTPKGKERAD GSGYGSTLPP EKLPYLVELS PGRRNHFAYY NYHTYEDGSD SRDKPKLYRL 430 440 450 460 470 480 QLSVTEVGTE KLDDNSIQLF GPGIQPHHCD LTNMDGVVTV TPRSMDAETY VEGQRISETT 490 500 510 520 530 540 MLQSGMKVQF GASHVFKFVD PSQDHALAKR SVDGGLMVKG PRHKPGIVQE TTFDLGGDIH 550 560 570 580 590 600 SGTALPTSKS TTRLDSDRVS SASSTAERGM VKPMIRVEQQ PDYRRQESRT QDASGPELIL 610 620 630 640 650 660 PASIEFRESS EDSFLSAIIN YTNSSTVHFK LSPTYVLYMA CRYVLSNQYR PDISPTERTH 670 680 690 700 710 720 KVIAVVNKMV SMMEGVIQKQ KNIAGALAFW MANASELLNF IKQDRDLSRI TLDAQDVLAH 730 740 750 760 770 780 LVQMAFKYLV HCLQSELNNY MPAFLDDPEE NSLQRPKIDD VLHTLTGAMS LLRRCRVNAA 790 800 810 820 830 840 LTIQLFSQLF HFINMWLFNR LVTDPDSGLC SHYWGAIIRQ QLGHIEAWAE KQGLELAADC 850 860 870 880 890 900 HLSRIVQATT LLTMDKYAPD DIPNINSTCF KLNSLQLQAL LQNYHCAPDE PFIPTDLIEN 910 920 930 940 950 960 VVTVAENTAD ELARSDGREV QLEEDPDLQL PFLLPEDGYS CDVVRNIPNG LQEFLDPLCQ 970 980 990 1000 1010 1020 RGFCRLIPHT RSPGTWTIYF EGADYESHLL RENTELAQPL RKEPEIITVT LKKQNGMGLS 1030 1040 1050 1060 1070 1080 IVAAKGAGQD KLGIYVKSVV KGGAADVDGR LAAGDQLLSV DGRSLVGLSQ ERAAELMTRT 1090 1100 1110 1120 1130 1140 SSVVTLEVAK QGAIYHGLAT LLNQPSPMMQ RISDRRGSGK PRPKSEGFEL YNNSTQNGSP 1150 1160 1170 1180 1190 1200 ESPQLPWAEY SEPKKLPGDD RLMKNRADHR SSPNVANQPP SPGGKSAYAS GTTAKITSVS 1210 1220 1230 1240 1250 1260 TGNLCTEEQT PPPRPEAYPI PTQTYTREYF TFPASKSQDR MAPPQNQWPN YEEKPHMHTD 1270 1280 1290 1300 1310 1320 SNHSSIAIQR VTRSQEELRE DKAYQLERHR IEAAMDRKSD SDMWINQSSS LDSSTSSQEH 1330 1340 1350 1360 1370 1380 LNHSSKSVTP ASTLTKSGPG RWKTPAAIPA TPVAVSQPIR TDLPPPPPPP PVHYAGDFDG 1390 1400 1410 1420 1430 1440 MSMDLPLPPP PSANQIGLPS AQVAAAERRK REEHQRWYEK EKARLEEERE RKRREQERKL 1450 1460 1470 1480 1490 1500 GQMRTQSLNP APFSPLTAQQ MKPEKPSTLQ RPQETVIREL QPQQQPRTIE RRDLQYITVS 1510 1520 1530 1540 1550 1560 KEELSSGDSL SPDPWKRDAK EKLEKQQQMH IVDMLSKEIQ ELQSKPDRSA EESDRLRKLM 1570 1580 1590 1600 1610 1620 LEWQFQKRLQ ESKQKDEDDE EEEDDDVDTM LIMQRLEAER RARTAMPAIS VLDLLQDEER 1630 1640 1650 1660 1670 1680 RRQQQLEEMR KREAEDRARQ EEERRRQEEE RTKRDAEEKR RQEEGYYSRL EAERRRQHDE 1690 1700 1710 1720 1730 1740 AARRLLEPEA PGLCRPPLPR DYEPPSPSPA PGAPPPPPQR NASYLKTQVL SPDSLFTAKF 1750 1760 1770 1780 1790 1800 VAYNEEEEEE DCSLAGPNSY PGSTGAAVGA HDACRDAKEK RSKSQDADSP GSSGAPENLT 1810 1820 1830 FKERQRLFSQ GQDVSNKVKA SRKLTELENE LNTK

References

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[2]	"Complete genomic structure, DNA polymorphisms, and alternative splicing of the human AF-6 gene." Saito S., Matsushima M., Shirahama S., Minaguchi T., Kanamori Y., Minami M., Nakamura Y. DNA Res. 5:115-120(1998) [PubMed: 9679199] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1; 2 AND 3). Tissue: Fetal brain.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Homo sapiens protein coding cDNA." Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 13-1824 (ISOFORM 6). Tissue: Brain.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 335-1824 (ISOFORM 5). Tissue: Amygdala.
[7]	"Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin." Reymond N., Borg J.-P., Lecocq E., Adelaide J., Campadelli-Fiume G., Dubreuil P., Lopez M. Gene 255:347-355(2000) [PubMed: 11024295] [Abstract] Cited for: INTERACTION WITH PVRL3.
[8]	"Nectin and afadin: novel organizers of intercellular junctions." Takai Y., Nakanishi H. J. Cell Sci. 116:17-27(2003) [PubMed: 12456712] [Abstract] Cited for: REVIEW ON INTERACTION.
[9]	"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed: 15144186] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1172 AND SER-1173, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1182 AND SER-1201, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1107, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1698, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-94, MASS SPECTROMETRY. Tissue: Lung carcinoma.
[14]	"Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction." Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S., Li S.-J., Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R. Mol. Cell. Proteomics 6:1952-1967(2007) [PubMed: 17693683] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1182, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[15]	"Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M. Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007) [PubMed: 17389395] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1230, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[16]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1696, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[17]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216; SER-1107; SER-1182; THR-1232; SER-1721; SER-1779 AND SER-1799. Tissue: Cervix carcinoma.
[18]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216; SER-1172; SER-1173; SER-1182; THR-1330; SER-1512 AND SER-1721, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[19]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216; SER-1083; SER-1182; SER-1721 AND SER-1799. Tissue: Leukemic T-cell.
[20]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[21]	"Solution structure of AF-6 PDZ domain and its interaction with the C-terminal peptides from Neurexin and Bcr." Zhou H., Xu Y., Yang Y., Huang A., Wu J., Shi Y. J. Biol. Chem. 280:13841-13847(2005) [PubMed: 15684424] [Abstract] Cited for: STRUCTURE BY NMR OF 1003-1094 IN COMPLEX WITH NRXN1 AND BCR.
[22]	"Structure of AF-6 PDZ domain." Joshi M., Boisguerin P., Leitner D., Volkmer-Engert R., Moelling K., Schade M., Schmieder P., Krause G., Oschkinat H. Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1001-1095.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U02478 mRNA. Translation: AAC50059.1.
AB011399 Genomic DNA. Translation: BAA32483.1.
AB011399 Genomic DNA. Translation: BAA32484.1.
AB011399 Genomic DNA. Translation: BAA32485.1.
AL009178, AL049698, AL731868 Genomic DNA. Translation: CAI20900.1.
AL009178, AL049698, AL731868 Genomic DNA. Translation: CAI20902.1.
AL731868, AL009178, AL049698 Genomic DNA. Translation: CAI40824.1.
AL731868, AL009178, AL049698 Genomic DNA. Translation: CAI40826.1.
AL049698, AL009178, AL731868 Genomic DNA. Translation: CAI42812.1.
AL049698, AL009178, AL731868 Genomic DNA. Translation: CAI42814.1.
CH471051 Genomic DNA. Translation: EAW47482.1.
AB209420 mRNA. Translation: BAD92657.1.
AL161973 mRNA. Translation: CAB82312.1. Frameshift.

IPI

IPI00023461.
IPI00216505.
IPI00398718.
IPI00759546.
IPI00953593.
IPI01014668.

PIR

T47137.

RefSeq

NP_001035089.1. NM_001040000.2.
NP_001193937.1. NM_001207008.1.

UniGene

Hs.614974.
Hs.708726.
Hs.728849.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1T2M	NMR	-	A	1003-1094	[»]
1XZ9	NMR	-	A	1001-1096	[»]
2AIN	NMR	-	A	1003-1094	[»]
2EXG	NMR	-	A	1001-1096	[»]

ProteinModelPortal

P55196.

SMR

P55196. Positions 11-138, 249-348, 374-503, 1001-1096.

ModBase

Search...

Protein-protein interaction databases

IntAct

P55196. 5 interactions.

MINT

MINT-90828.

STRING

P55196.

PTM databases

PhosphoSite

P55196.

Polymorphism databases

DMDM

288558835.

Proteomic databases

PRIDE

P55196.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000351017; ENSP00000252692; ENSG00000130396.

GeneID

4301.

KEGG

hsa:4301.

UCSC

uc003qwd.1. human.

Organism-specific databases

CTD

4301.

GeneCards

GC06P168227.

HGNC

HGNC:7137. MLLT4.

HPA

CAB013496.
HPA030212.
HPA030213.
HPA030214.
HPA030215.

MIM

159559. gene.

neXtProt

NX_P55196.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG14808.

GeneTree

ENSGT00390000006525.

HOVERGEN

HBG050463.

OMA

RWKTPAA.

Enzyme and pathway databases

Reactome

REACT_111155. Cell-Cell communication.

Gene expression databases

ArrayExpress

P55196.

Bgee

P55196.

CleanEx

HS_MLLT4.

Genevestigator

P55196.

GermOnline

ENSG00000130396. Homo sapiens.

Family and domain databases

InterPro

IPR018444. Dil_domain.
IPR002710. Dilute.
IPR000253. FHA_dom.
IPR001478. PDZ/DHR/GLGF.
IPR000159. Ras-assoc.
IPR008984. SMAD_FHA_domain.
[Graphical view]

Gene3D

G3DSA:2.60.200.20. FHA. 1 hit.

KO

K05702.

Pfam

PF01843. DIL. 1 hit.
PF00498. FHA. 1 hit.
PF00595. PDZ. 1 hit.
PF00788. RA. 2 hits.
[Graphical view]

SMART

SM00240. FHA. 1 hit.
SM00228. PDZ. 1 hit.
SM00314. RA. 2 hits.
[Graphical view]

SUPFAM

SSF50156. PDZ. 1 hit.
SSF49879. SMAD_FHA. 1 hit.

PROSITE

PS51126. DILUTE. 1 hit.
PS50006. FHA_DOMAIN. False negative.
PS50106. PDZ. 1 hit.
PS50200. RA. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

16931.

SOURCE

Search...

Entry information

Entry name

AFAD_HUMAN

Accession

Primary (citable) accession number: P55196
Secondary accession number(s): O75087 , O75088, O75089, Q59FP0, Q5TIG6, Q5TIG7, Q9NSN7, Q9NU92

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	February 9, 2010
Last modified:	January 25, 2012
This is version 130 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.