Reviewed, UniProtKB/Swiss-Prot P55195 (PUR6_VIGAC)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase, chloroplastic
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:

PURKE

Organism

Vigna aconitifolia (Mothbean)

Taxonomic identifier

3918 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length	557 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subcellular location	Plastid › chloroplast Probable.
Sequence similarities	In the C-terminal section; belongs to the AIR carboxylase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – ?		Chloroplast Potential
	Chain	? – 557		Phosphoribosylaminoimidazole carboxylase, chloroplastic		PRO_0000029879
Regions
	Domain	108 – 293	186	ATP-grasp
	Nucleotide binding	132 – 189	58	ATP By similarity
	Region	? – 386		AIR carboxylase ATPase subunit
	Region	387 – 557	171	AIR carboxylase catalytic subunit
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P55195-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: E88FEAB6415B4EC2	FASTA	557	60,942
	10 20 30 40 50 60 GLYEVVVGVL GGGQLGRMMC QAASQMAIKV MVLDPQENCP ASSLSYHHMV GSFDESTKVE 70 80 90 100 110 120 EFAKRCGVLT VEIEHVDVDT LEKLEKQGVD CQPKASTVRI IQDKYQQKVA LLPAWIPLPE 130 140 150 160 170 180 FMKIDDLKAK KWDSLDIHFM IKSRRLAYDG RGNFVAKSEE ELSSAVDALG GFDRGLYAEK 190 200 210 220 230 240 WAPFVKELAV IVARGRDNSI SCYPVVELFT GHICHIVKSP ANVNWKTREL AIEVAFNAVK 250 260 270 280 290 300 SLEVPGVFAV ELFLTKEGEI LLNEVAPRPH NSGHHTIESC HTSQFEQHLP AVVGLPLGDP 310 320 330 340 350 360 SMKTPAAIMY NILGEEEGEH GFQLAHQLMK RAMTIPGASV HWYDKPEMRK QRKMCHITIV 370 380 390 400 410 420 GSSLSSIESN LAILLEGKGL HDKTAVCSTL LGFIMGSDSD LPVMKSAAEM MEMFGVPHEV 430 440 450 460 470 480 RIVSAHRTPE LMFCYASSAH ERGYQVIIAG AGGAAHLPGM VASLTPLPVV GVPVRASTLD 490 500 510 520 530 540 GLDSLLSIVQ MPRGVPVATV AVNNATNAGL LAVRMLGVAN DNLLSRMSQY QEDQKEAVLR 550 EGDKLEKHGW ESYLKNS

References

[1]

"Structural organization of de novo purine biosynthesis enzymes in plants: 5-aminoimidazole ribonucleotide carboxylase and 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase cDNAs from Vigna aconitifolia." Chapman K.A., Delauney A.J., Kim J.H., Verma D.P.S. Plant Mol. Biol. 24:389-395(1994) [PubMed: 8111040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Root nodule.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L22584 mRNA. Translation: AAC37400.1.
PIR	S43322.
3D structure databases
HSSP	HSSP built from PDB template 1O4V based on UniProtKB Q9WYS7.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.1.1.21. 18771.
Family and domain databases
InterPro	IPR016301. AIR_COase. IPR005875. AIR_COase_ATPase-su. IPR000031. AIR_COase_core. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF00731. AIRC. 1 hit. PF02222. ATP-grasp. 1 hit. [Graphical view]
PIRSF	PIRSF001340. AIR_carboxylase. 1 hit.
ProDom	PD002193. AIR_carboxyl. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01162. purE. 1 hit. TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR6_VIGAC

Accession

Primary (citable) accession number: P55195

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	September 1, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)