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Protein

SH3 domain-binding protein 1

Gene

Sh3bp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase activating protein (GAP) which specifically converts GTP-bound Rho-type GTPases including RAC1 and CDC42 in their inactive GDP-bound form (PubMed:7621827). By specifically inactivating RAC1 at the leading edge of migrating cells, it regulates the spatiotemporal organization of cell protrusions which is important for proper cell migration. Also negatively regulates CDC42 in the process of actin remodeling and the formation of epithelial cell junctions. Through its GAP activity toward RAC1 and/or CDC42 plays a specific role in phagocytosis of large particles. Specifically recruited by a PI3 kinase/PI3K-dependent mechanism to sites of large particles engagement, inactivates RAC1 and/or CDC42 allowing the reorganization of the underlying actin cytoskeleton required for engulfment. It also plays a role in angiogenesis and the process of repulsive guidance as part of a semaphorin-plexin signaling pathway. Following the binding of PLXND1 to extracellular SEMA3E it dissociates from PLXND1 and inactivates RAC1, inducing the intracellular reorganization of the actin cytoskeleton and the collapse of cells (By similarity).By similarity1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB
  • semaphorin receptor binding Source: MGI
  • SH3 domain binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
Biological processPhagocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
SH3 domain-binding protein 1Imported
Short name:
3BP-11 Publication
Gene namesi
Name:Sh3bp1Imported
Synonyms:3bp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:104603 Sh3bp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Nucleus, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000567241 – 680SH3 domain-binding protein 1Add BLAST680

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei241PhosphoserineBy similarity1
Modified residuei262PhosphoserineBy similarity1
Modified residuei535PhosphoserineCombined sources1
Modified residuei582PhosphoserineCombined sources1
Modified residuei592PhosphothreonineCombined sources1
Modified residuei632PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP55194
MaxQBiP55194
PaxDbiP55194
PeptideAtlasiP55194
PRIDEiP55194

PTM databases

iPTMnetiP55194
PhosphoSitePlusiP55194

Expressioni

Tissue specificityi

Expressed in all tissues examined. Highest levels found in spleen and brain, lowest in heart and liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000022436
CleanExiMM_SH3BP1

Interactioni

Subunit structurei

Interacts with RAC1 (PubMed:10508610). Interacts with the exocyst via EXOC4 and EXOC8; required for the localization of both SH3BP1 and the exocyst to the leading edge of migrating cells. Interacts with CD2AP and CGNL1; probably part of a complex at cell junctions. Interacts with CAPZA1; recruits CAPZA1 to forming cell junctions. May interact with AFDN. Interacts with PLXND1; they dissociate upon SEMA3E binding to PLXND1 allowing SH3BP1 to transduce downstream signal through RAC1 inactivation (By similarity). Interacts with ABL1, GRB2 and SRC (via SH3 domain) (PubMed:8438166, PubMed:1379745).By similarity3 Publications

GO - Molecular functioni

  • semaphorin receptor binding Source: MGI
  • SH3 domain binding Source: MGI

Protein-protein interaction databases

IntActiP55194, 3 interactors
MINTiP55194
STRINGi10090.ENSMUSP00000052181

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ABOX-ray2.00C/D607-616[»]
ProteinModelPortaliP55194
SMRiP55194
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55194

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 262BARPROSITE-ProRule annotationAdd BLAST182
Domaini276 – 469Rho-GAPPROSITE-ProRule annotationAdd BLAST194

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 275Interaction with CGNL1By similarityAdd BLAST275
Regioni470 – 680Interaction with CD2APBy similarityAdd BLAST211

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi607 – 616SH3-binding1 Publication10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi500 – 672Pro-richPROSITE-ProRule annotationAdd BLAST173

Domaini

The BAR domain mediates interaction with the exocyst components EXOC4 and EXOC8 and is required for the function in cell migration. It also mediates the interaction with PLXND1.By similarity

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiKOG4270 Eukaryota
ENOG410XRR2 LUCA
GeneTreeiENSGT00760000118863
HOGENOMiHOG000179193
HOVERGENiHBG000015
InParanoidiP55194
KOiK20652
OrthoDBiEOG091G02YP

Family and domain databases

Gene3Di1.10.555.10, 1 hit
1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR004148 BAR_dom
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
PfamiView protein in Pfam
PF03114 BAR, 1 hit
PF00620 RhoGAP, 1 hit
SMARTiView protein in SMART
SM00721 BAR, 1 hit
SM00324 RhoGAP, 1 hit
SUPFAMiSSF103657 SSF103657, 2 hits
SSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS51021 BAR, 1 hit
PS50238 RHOGAP, 1 hit

Sequencei

Sequence statusi: Complete.

P55194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKRQLHRMR QLAHTGSSGR TPETAEFLGE DLLQVEQRLE PAKRAAHNVH
60 70 80 90 100
KRLQACLQGQ SGADMDKRVK KLPLMALSTT MAESFKELDP DSSMGKALEM
110 120 130 140 150
TCAIQNQLAR ILAEFEMTLE RDVLQPLSRL SEEELPAILK HKKSLQKLVS
160 170 180 190 200
DWNTLKSRLS QAAKNSGSNQ GLGGASGSHT HTTTANKVEM LKEEEEELKK
210 220 230 240 250
KVEQCKDEYL ADLYHFSTKE DSYANYFIHL LEIQADYHRK SLTSLDTALA
260 270 280 290 300
ELRDNHSQAD HSPLTTAAPF SRVYGVSLRT HLQDLGRDIA LPIEACVLLL
310 320 330 340 350
LSEGMQEEGL FRLAAGASVL KRLKQTMASD PHSLEEFCSD PHAVAGALKS
360 370 380 390 400
YLRELPEPLM TSDLYDDWMR AASLKEPGAR LEALHDVCSR LPQENFNNLR
410 420 430 440 450
YLMKFLALLA EEQDVNKMTP SNIAIVLGPN LLWPPEKEGD QAQLDAASVS
460 470 480 490 500
SIQVVGVVEA LIQNADTLFP GDINFNVSGI FPGLAPQEKV SSQQVSEELP
510 520 530 540 550
PVTVPAPATT PAPTPAPASM AVRERTEADL PKPTSPKVSR NPTETAASAE
560 570 580 590 600
DMTRKTKRPA PARPTMPPPQ PSSTRSSPPA PSLPPGSVSP GTPQALPRRL
610 620 630 640 650
VGTSLRAPTM PPPLPPVPPQ PARRQSRRLP ASPVISNMPA QVDQGVATED
660 670 680
RGGPEAVGGH PPPPALPPQP RPRGLISETE
Length:680
Mass (Da):74,172
Last modified:October 25, 2017 - v3
Checksum:i97AB443D4350DE16
GO

Sequence cautioni

The sequence AAH04598 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA61011 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126 – 128PLS → RSG in CAA61011 (PubMed:7621827).Curated3
Sequence conflicti257S → N in CAA61011 (PubMed:7621827).Curated1
Sequence conflicti257S → N in AAH04598 (PubMed:15489334).Curated1
Sequence conflicti306Q → QE in CAA61011 (PubMed:7621827).Curated1
Sequence conflicti340D → G in CAA61011 (PubMed:7621827).Curated1
Sequence conflicti515P → L in CAA61011 (PubMed:7621827).Curated1
Sequence conflicti515P → L in AAH04598 (PubMed:15489334).Curated1
Sequence conflicti652G → E in CAA61011 (PubMed:7621827).Curated1
Sequence conflicti652G → E in AAH04598 (PubMed:15489334).Curated1
Sequence conflicti671 – 672RP → PA in CAA61011 (PubMed:7621827).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87671 mRNA Translation: CAA61011.1 Different initiation.
AL592169 Genomic DNA No translation available.
BC004598 mRNA Translation: AAH04598.1 Different initiation.
CCDSiCCDS84176.1
PIRiS56144
RefSeqiNP_001303613.1, NM_001316684.1
UniGeneiMm.4462

Genome annotation databases

EnsembliENSMUST00000001226; ENSMUSP00000001226; ENSMUSG00000022436
GeneIDi20401
KEGGimmu:20401

Similar proteinsi

Entry informationi

Entry namei3BP1_MOUSE
AccessioniPrimary (citable) accession number: P55194
Secondary accession number(s): A2A5V4, E9QMQ2, Q99KK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 25, 2017
Last modified: May 23, 2018
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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