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Protein

SH3 domain-binding protein 1

Gene

Sh3bp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac.

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB-KW
  • SH3 domain binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Names & Taxonomyi

Protein namesi
Recommended name:
SH3 domain-binding protein 1
Short name:
3BP-1
Gene namesi
Name:Sh3bp1
Synonyms:3bp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104603. Sh3bp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 601601SH3 domain-binding protein 1PRO_0000056724Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei182 – 1821PhosphoserineBy similarity
Modified residuei456 – 4561PhosphoserineCombined sources
Modified residuei503 – 5031PhosphoserineCombined sources
Modified residuei513 – 5131PhosphothreonineCombined sources
Modified residuei553 – 5531PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP55194.
MaxQBiP55194.
PaxDbiP55194.
PeptideAtlasiP55194.
PRIDEiP55194.

PTM databases

iPTMnetiP55194.
PhosphoSiteiP55194.

Expressioni

Tissue specificityi

Expressed in all tissues examined. Highest levels found in spleen and brain, lowest in heart and liver.

Gene expression databases

CleanExiMM_SH3BP1.

Interactioni

GO - Molecular functioni

  • SH3 domain binding Source: MGI

Protein-protein interaction databases

IntActiP55194. 2 interactions.
MINTiMINT-274688.
STRINGi10090.ENSMUSP00000052181.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ABOX-ray2.00C/D528-537[»]
ProteinModelPortaliP55194.
SMRiP55194. Positions 192-392.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55194.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 182182BARPROSITE-ProRule annotationAdd
BLAST
Domaini196 – 390195Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi529 – 5379SH3-binding

Sequence similaritiesi

Contains 1 BAR domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiKOG4270. Eukaryota.
ENOG410XRR2. LUCA.
HOGENOMiHOG000179193.
HOVERGENiHBG000015.
InParanoidiP55194.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR004148. BAR_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAESFKELDP DSSMGKALEM TCAIQNQLAR ILAEFEMTLE RDVLQPLSRL
60 70 80 90 100
SEEELPAILK HKKSLQKLVS DWNTLKSRLS QAAKNSGSNQ GLGGASGSHT
110 120 130 140 150
HTTTANKVEM LKEEEEELKK KVEQCKDEYL ADLYHFSTKE DSYANYFIHL
160 170 180 190 200
LEIQADYHRK SLTSLDTALA ELRDNHSQAD HSPLTTAAPF SRVYGVSLRT
210 220 230 240 250
HLQDLGRDIA LPIEACVLLL LSEGMQEEEG LFRLAAGASV LKRLKQTMAS
260 270 280 290 300
DPHSLEEFCS DPHAVAGALK SYLRELPEPL MTSDLYDDWM RAASLKEPGA
310 320 330 340 350
RLEALHDVCS RLPQENFNNL RYLMKFLALL AEEQDVNKMT PSNIAIVLGP
360 370 380 390 400
NLLWPPEKEG DQAQLDAASV SSIQVVGVVE ALIQNADTLF PGDINFNVSG
410 420 430 440 450
IFPGLAPQEK VSSQQVSEEL PPVTVPAPAT TPAPTPAPAS MAVRERTEAD
460 470 480 490 500
LPKPTSPKVS RNPTETAASA EDMTRKTKRP APARPTMPPP QPSSTRSSPP
510 520 530 540 550
APSLPPGSVS PGTPQALPRR LVGTSLRAPT MPPPLPPVPP QPARRQSRRL
560 570 580 590 600
PASPVISNMP AQVDQGVATE DRGGPEAVGG HPPPPALPPQ PRPRGLISET

E
Length:601
Mass (Da):65,285
Last modified:July 27, 2011 - v2
Checksum:iAB999C8F54B6521E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 483PLS → RSG in CAA61011 (PubMed:7621827).Curated
Sequence conflicti177 – 1771S → N in CAA61011 (PubMed:7621827).Curated
Sequence conflicti177 – 1771S → N in AAH04598 (PubMed:15489334).Curated
Sequence conflicti227 – 2271Missing in AAH04598 (PubMed:15489334).Curated
Sequence conflicti261 – 2611D → G in CAA61011 (PubMed:7621827).Curated
Sequence conflicti436 – 4361P → L in CAA61011 (PubMed:7621827).Curated
Sequence conflicti436 – 4361P → L in AAH04598 (PubMed:15489334).Curated
Sequence conflicti573 – 5731G → E in CAA61011 (PubMed:7621827).Curated
Sequence conflicti573 – 5731G → E in AAH04598 (PubMed:15489334).Curated
Sequence conflicti592 – 5932RP → PA in CAA61011 (PubMed:7621827).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87671 mRNA. Translation: CAA61011.1.
AL592169 Genomic DNA. No translation available.
BC004598 mRNA. Translation: AAH04598.1.
PIRiS56144.
UniGeneiMm.4462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87671 mRNA. Translation: CAA61011.1.
AL592169 Genomic DNA. No translation available.
BC004598 mRNA. Translation: AAH04598.1.
PIRiS56144.
UniGeneiMm.4462.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ABOX-ray2.00C/D528-537[»]
ProteinModelPortaliP55194.
SMRiP55194. Positions 192-392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP55194. 2 interactions.
MINTiMINT-274688.
STRINGi10090.ENSMUSP00000052181.

PTM databases

iPTMnetiP55194.
PhosphoSiteiP55194.

Proteomic databases

EPDiP55194.
MaxQBiP55194.
PaxDbiP55194.
PeptideAtlasiP55194.
PRIDEiP55194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:104603. Sh3bp1.

Phylogenomic databases

eggNOGiKOG4270. Eukaryota.
ENOG410XRR2. LUCA.
HOGENOMiHOG000179193.
HOVERGENiHBG000015.
InParanoidiP55194.

Miscellaneous databases

EvolutionaryTraceiP55194.
PROiP55194.
SOURCEiSearch...

Gene expression databases

CleanExiMM_SH3BP1.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR004148. BAR_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "3BP-1, an SH3 domain binding protein, has GAP activity for Rac and inhibits growth factor-induced membrane ruffling in fibroblasts."
    Cicchetti P., Ridley A.J., Zheng Y., Cerione R.A., Baltimore D.
    EMBO J. 14:3127-3135(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Identification of a protein that binds to the SH3 region of Abl and is similar to Bcr and GAP-rho."
    Cicchetti P., Mayer B.J., Thiel G., Baltimore D.
    Science 257:803-806(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 263-601.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-553, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456; SER-503 AND THR-513, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung, Spleen and Testis.
  7. "Identification of a ten-amino acid proline-rich SH3 binding site."
    Ren R., Mayer B.J., Cicchetti P., Baltimore D.
    Science 259:1157-1161(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 528-537.

Entry informationi

Entry namei3BP1_MOUSE
AccessioniPrimary (citable) accession number: P55194
Secondary accession number(s): E9QMQ2, Q99KK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.