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Protein

Nck-associated protein 1

Gene

Nckap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1 (By similarity).By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
Nck-associated protein 1
Short name:
NAP 1
Alternative name(s):
Membrane-associated protein HEM-2
p125Nap1
Gene namesi
Name:Nckap1
Synonyms:Hem2, Nap1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61939. Nckap1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei995 – 101521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 11281127Nck-associated protein 1PRO_0000216174Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP55161.
PRIDEiP55161.

PTM databases

iPTMnetiP55161.
PhosphoSiteiP55161.

Expressioni

Tissue specificityi

Preferentially expressed in brain, heart, liver and testis.

Interactioni

Subunit structurei

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Associates preferentially with the first SH3 domain of NCK. Interacts with NYAP1, NYAP2 and MYO16 (By similarity).By similarity

Protein-protein interaction databases

BioGridi248630. 2 interactions.
IntActiP55161. 1 interaction.
MINTiMINT-1530093.
STRINGi10116.ENSRNOP00000059233.

Family & Domainsi

Sequence similaritiesi

Belongs to the HEM-1/HEM-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1917. Eukaryota.
ENOG410XPQI. LUCA.
HOGENOMiHOG000231880.
HOVERGENiHBG006344.
InParanoidiP55161.
KOiK05750.
PhylomeDBiP55161.

Family and domain databases

InterProiIPR019137. Nck-associated_protein-1.
[Graphical view]
PfamiPF09735. Nckap1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRSVLQPSQ QKLAEKLTIL NDRGVGMLTR LYNIKKACGD PKAKPSYLID
60 70 80 90 100
KNLESAVKFI VRKFPAVETR NNNQQLAQLQ KEKSEILKNL ALYYFTFVDV
110 120 130 140 150
MEFKDHVCDL LNTIDVCQVF FDITVNFDLT KNYLDLTVTY TTLMILLSRI
160 170 180 190 200
EERKAIIGLY NYAHEMTHGA SDREYPRLGQ MIVDYEHPLK KMMEEFVPHS
210 220 230 240 250
KSLSDALISL QMVYPRRNLS ADQWRNAQLL SLISAPSTML NPAQSDTMPC
260 270 280 290 300
EYLSLDAMEK WIIFGFILCH GMLNTEATAL NLWKLALQSS SCLSLFRDEV
310 320 330 340 350
FHIHKAAEDL FVNIRGYNKR INDIRECKEA AVSHAGSMHR ERRKFLRSAL
360 370 380 390 400
KELATVLSDQ PGLLGPKALF VFMALSFARD EIIWLLRHAD NMPKKSADDF
410 420 430 440 450
IDKHIAELIF YMEELRAHVR KYGPVMQRYY VQYLSGFDAV VLNELVQNLS
460 470 480 490 500
VCPEDESIIM SSFVNTMTSL SVKQVEDGEV FDFRGMRLDW FRLQAYTSVS
510 520 530 540 550
KASLSLADHR ELGKMMNTII FHTKMVDSLV EMLVETSDLS IFCFYSRAFE
560 570 580 590 600
KMFQQCLELP SQSRYSIAFP LLCTHFMSCT HELCPEERHH IGDRSLSLCN
610 620 630 640 650
MFLDEMAKQA RNLITDICTE QCTLSDQLLP KHCAKTISQA VNKKSKKQTG
660 670 680 690 700
KKGEPEREKP GVESMRKNRL VVTNLDKLHT ALSELCFSIN YVPNMAVWEH
710 720 730 740 750
TFTPREYLTS HLEIRFTKSI VGMTMYNQAT QEIAKPSELL TSVREYMTVL
760 770 780 790 800
QSIENYVQID ITRVFNNVLL QQTQHLDSHG EPTITSLYTN WYLETLLRQV
810 820 830 840 850
SNGHIAYFPA MKAFVNLPTE NELTFNAEEY SDISEMRSLS ELLGPYGMKF
860 870 880 890 900
LSESLMWHIS SQVAELKKLV VENVDVLTQM RTSFDKPDQM AALFKRLSSV
910 920 930 940 950
DSVLKRMTII GVILSFRSLA QEALRDVLSY HIPFLVSSIE DFKDHIPRET
960 970 980 990 1000
DMKVAMNVYE LSSAAGLPCE IDPALVVALS SQKSENISPE EEYKIACLLM
1010 1020 1030 1040 1050
VFVAVSLPTL ASNVMSQYSP AIEGHCNNIH CLAKAINQIA AALFTIHKGS
1060 1070 1080 1090 1100
IEDRLKEFLA LASSSLLKIG QETDKTTTRN RESVYLLLDM IVQESPFLTM
1110 1120
DLLESCFPYV LLRNAYHAVY KQSVTSSA
Length:1,128
Mass (Da):128,865
Last modified:October 1, 1996 - v1
Checksum:i95A888A0CB0E3114
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 3029SRSVL…GMLTR → MLRGTEIVYIKFVLKFFKRN S (PubMed:7643388).CuratedAdd
BLAST
Sequence conflicti90 – 901L → M in CAA56333 (PubMed:7643388).Curated
Sequence conflicti102 – 1043EFK → DLR in CAA56333 (PubMed:7643388).Curated
Sequence conflicti115 – 1151D → A in CAA56333 (PubMed:7643388).Curated
Sequence conflicti152 – 1521E → G in CAA56333 (PubMed:7643388).Curated
Sequence conflicti158 – 1581G → R in CAA56333 (PubMed:7643388).Curated
Sequence conflicti170 – 1701A → G in CAA56333 (PubMed:7643388).Curated
Sequence conflicti194 – 1941E → V in CAA56333 (PubMed:7643388).Curated
Sequence conflicti300 – 3001Missing in CAA56333 (PubMed:7643388).Curated
Sequence conflicti513 – 5142GK → ER in CAA56333 (PubMed:7643388).Curated
Sequence conflicti618 – 6181C → Y in CAA56333 (PubMed:7643388).Curated
Sequence conflicti658 – 6592EK → KS in CAA56333 (PubMed:7643388).Curated
Sequence conflicti745 – 7451E → A in CAA56333 (PubMed:7643388).Curated
Sequence conflicti805 – 8062IA → MP in CAA56333 (PubMed:7643388).Curated
Sequence conflicti844 – 8441G → A in CAA56333 (PubMed:7643388).Curated
Sequence conflicti915 – 9151S → C in CAA56333 (PubMed:7643388).Curated
Sequence conflicti983 – 99311KSENISPEEEY → NQRTLVQEEDI in CAA56333 (PubMed:7643388).CuratedAdd
BLAST
Sequence conflicti1033 – 105220AKAIN…KGSIE → PKPSTKLLLLCLQFTKEALK in CAA56333 (PubMed:7643388).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84346 mRNA. Translation: BAA12319.1.
X80029 mRNA. Translation: CAA56333.1.
PIRiS57833.
RefSeqiNP_113806.1. NM_031618.1.
UniGeneiRn.2611.

Genome annotation databases

GeneIDi58823.
KEGGirno:58823.
UCSCiRGD:61939. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84346 mRNA. Translation: BAA12319.1.
X80029 mRNA. Translation: CAA56333.1.
PIRiS57833.
RefSeqiNP_113806.1. NM_031618.1.
UniGeneiRn.2611.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248630. 2 interactions.
IntActiP55161. 1 interaction.
MINTiMINT-1530093.
STRINGi10116.ENSRNOP00000059233.

PTM databases

iPTMnetiP55161.
PhosphoSiteiP55161.

Proteomic databases

PaxDbiP55161.
PRIDEiP55161.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi58823.
KEGGirno:58823.
UCSCiRGD:61939. rat.

Organism-specific databases

CTDi10787.
RGDi61939. Nckap1.

Phylogenomic databases

eggNOGiKOG1917. Eukaryota.
ENOG410XPQI. LUCA.
HOGENOMiHOG000231880.
HOVERGENiHBG006344.
InParanoidiP55161.
KOiK05750.
PhylomeDBiP55161.

Miscellaneous databases

PROiP55161.

Family and domain databases

InterProiIPR019137. Nck-associated_protein-1.
[Graphical view]
PfamiPF09735. Nckap1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCKP1_RAT
AccessioniPrimary (citable) accession number: P55161
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.