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P55160

- NCKPL_HUMAN

UniProt

P55160 - NCKPL_HUMAN

Protein

Nck-associated protein 1-like

Gene

NCKAP1L

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 3 (16 Dec 2008)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. protein complex binding Source: UniProtKB
    3. protein kinase activator activity Source: UniProtKB
    4. Rac GTPase activator activity Source: UniProtKB

    GO - Biological processi

    1. actin polymerization-dependent cell motility Source: UniProtKB
    2. B cell homeostasis Source: UniProtKB
    3. B cell receptor signaling pathway Source: UniProtKB
    4. chemotaxis Source: UniProtKB
    5. cortical actin cytoskeleton organization Source: UniProtKB
    6. erythrocyte development Source: UniProtKB
    7. maintenance of cell polarity Source: UniProtKB
    8. myeloid cell homeostasis Source: UniProtKB
    9. negative regulation of apoptotic process Source: UniProtKB
    10. negative regulation of interleukin-17 production Source: UniProtKB
    11. negative regulation of interleukin-6 production Source: UniProtKB
    12. negative regulation of myosin-light-chain-phosphatase activity Source: UniProtKB
    13. neutrophil chemotaxis Source: UniProtKB
    14. positive regulation of actin filament polymerization Source: UniProtKB
    15. positive regulation of B cell differentiation Source: UniProtKB
    16. positive regulation of B cell proliferation Source: UniProtKB
    17. positive regulation of CD4-positive, alpha-beta T cell differentiation Source: UniProtKB
    18. positive regulation of CD8-positive, alpha-beta T cell differentiation Source: UniProtKB
    19. positive regulation of cell adhesion mediated by integrin Source: UniProtKB
    20. positive regulation of erythrocyte differentiation Source: UniProtKB
    21. positive regulation of gamma-delta T cell differentiation Source: UniProtKB
    22. positive regulation of lymphocyte differentiation Source: UniProtKB
    23. positive regulation of neutrophil chemotaxis Source: UniProtKB
    24. positive regulation of phagocytosis, engulfment Source: UniProtKB
    25. positive regulation of phosphorylation Source: UniProtKB
    26. positive regulation of protein kinase activity Source: GOC
    27. positive regulation of Rac GTPase activity Source: GOC
    28. positive regulation of T cell proliferation Source: UniProtKB
    29. protein complex assembly Source: UniProtKB
    30. response to drug Source: UniProtKB
    31. T cell homeostasis Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nck-associated protein 1-like
    Alternative name(s):
    Hematopoietic protein 1
    Membrane-associated protein HEM-1
    Gene namesi
    Name:NCKAP1L
    Synonyms:HEM1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:4862. NCKAP1L.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. extracellular vesicular exosome Source: UniProt
    3. integral component of plasma membrane Source: UniProtKB
    4. membrane Source: UniProtKB
    5. SCAR complex Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29239.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11271127Nck-associated protein 1-likePRO_0000216175Add
    BLAST

    Proteomic databases

    MaxQBiP55160.
    PaxDbiP55160.
    PRIDEiP55160.

    PTM databases

    PhosphoSiteiP55160.

    Expressioni

    Tissue specificityi

    Expressed only in cells of hematopoietic origin.

    Gene expression databases

    ArrayExpressiP55160.
    BgeeiP55160.
    CleanExiHS_NCKAP1L.
    GenevestigatoriP55160.

    Organism-specific databases

    HPAiHPA039490.
    HPA040772.

    Interactioni

    Protein-protein interaction databases

    BioGridi109321. 4 interactions.
    MINTiMINT-1711041.
    STRINGi9606.ENSP00000293373.

    Structurei

    3D structure databases

    ProteinModelPortaliP55160.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei996 – 101621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HEM-1/HEM-2 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG287735.
    HOGENOMiHOG000231880.
    HOVERGENiHBG006344.
    InParanoidiP55160.
    KOiK05750.
    OMAiAFMALSF.
    OrthoDBiEOG7TJ3H0.
    PhylomeDBiP55160.
    TreeFamiTF313683.

    Family and domain databases

    InterProiIPR019137. Nck-associated_protein-1.
    [Graphical view]
    PfamiPF09735. Nckap1. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P55160-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLTSAYQHK LAEKLTILND RGQGVLIRMY NIKKTCSDPK SKPPFLLEKS     50
    MEPSLKYINK KFPNIDVRNS TQHLGPVHRE KAEIIRFLTN YYQSFVDVME 100
    FRDHVYELLN TIDACQCHFD INLNFDFTRS YLDLIVTYTS VILLLSRIED 150
    RRILIGMYNC AHEMLHGHGD PSFARLGQMV LEYDHPLKKL TEEFGPHTKA 200
    VSGALLSLHF LFVRRNQGAE QWRSAQLLSL ISNPPAMINP ANSDTMACEY 250
    LSVEVMERWI IIGFLLCHGC LNSNSQCQKL WKLCLQGSLY ITLIREDVLQ 300
    VHKVTEDLFS SLKGYGKRVA DIKESKEHVI ANSGQFHCQR RQFLRMAVKE 350
    LETVLADEPG LLGPKALFAF MALSFIRDEV TWLVRHTENV TKTKTPEDYA 400
    DSSIAELLFL LEGIRSLVRR HIKVIQQYHL QYLARFDALV LSDIIQNLSV 450
    CPEEESIIMS SFVSILSSLN LKQVDNGEKF EFSGLRLDWF RLQAYTSVAK 500
    APLHLHENPD LAKVMNLIVF HSRMLDSVEK LLVETSDLST FCFHLRIFEK 550
    MFAMTLEESA MLRYAIAFPL ICAHFVHCTH EMCPEEYPHL KNHGLHHCNS 600
    FLEELAKQTS NCVLEICAEQ RNLSEQLLPK HCATTISKAK NKKTRKQRQT 650
    PRKGEPERDK PGAESHRKNR SIVTNMDKLH LNLTELALTM NHVYSFSVFE 700
    HTIFPSEYLS SHLEARLNRA IVWLAGYNAT TQEIVRPSEL LAGVKAYIGF 750
    IQSLAQFLGA DASRVIRNAL LQQTQPLDSC GEQTITTLYT NWYLESLLRQ 800
    ASSGTIILSP AMQAFVSLPR EGEQNFSAEE FSDISEMRAL AELLGPYGMK 850
    FLSENLMWHV TSQIVELKKL VVENMDILVQ IRSNFSKPDL MASLLPQLTG 900
    AENVLKRMTI IGVILSFRAM AQEGLREVFS SHCPFLMGPI ECLKEFVTPD 950
    TDIKVTLSIF ELASAAGVGC DIDPALVAAI ANLKADTSSP EEEYKVACLL 1000
    LIFLAVSLPL LATDPSSFYS IEKDGYNNNI HCLTKAIIQV SAALFTLYNK 1050
    NIETHLKEFL VVASVSLLQL GQETDKLKTR NRESISLLMR LVVEESSFLT 1100
    LDMLESCFPY VLLRNAYREV SRAFHLN 1127
    Length:1,127
    Mass (Da):128,153
    Last modified:December 16, 2008 - v3
    Checksum:i9389BAB163BAEA45
    GO
    Isoform 2 (identifier: P55160-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-50: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,077
    Mass (Da):122,478
    Checksum:iCE2F51F1F17E2C83
    GO

    Sequence cautioni

    The sequence AAA35964.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti496 – 4961T → H in AAA35964. (PubMed:7643388)Curated
    Sequence conflicti768 – 7692NA → KP in AAA35964. (PubMed:7643388)Curated
    Sequence conflicti1060 – 10601L → V in AAA35964. (PubMed:7643388)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti391 – 3911T → A.
    Corresponds to variant rs7311877 [ dbSNP | Ensembl ].
    VAR_059316
    Natural varianti402 – 4021S → L.
    Corresponds to variant rs2270581 [ dbSNP | Ensembl ].
    VAR_049344

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5050Missing in isoform 2. 1 PublicationVSP_045191Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58285 mRNA. Translation: AAA35964.1. Different initiation.
    AK300783 mRNA. Translation: BAG62447.1.
    AC025570 Genomic DNA. No translation available.
    AC068789 Genomic DNA. No translation available.
    AC079313 Genomic DNA. No translation available.
    CH471054 Genomic DNA. Translation: EAW96787.1.
    BC093769 mRNA. Translation: AAH93769.1.
    BC093771 mRNA. Translation: AAH93771.1.
    CCDSiCCDS31813.1. [P55160-1]
    CCDS53799.1. [P55160-2]
    PIRiS36666.
    RefSeqiNP_001171905.1. NM_001184976.1. [P55160-2]
    NP_005328.2. NM_005337.4. [P55160-1]
    UniGeneiHs.182014.

    Genome annotation databases

    EnsembliENST00000293373; ENSP00000293373; ENSG00000123338. [P55160-1]
    ENST00000545638; ENSP00000445596; ENSG00000123338. [P55160-2]
    GeneIDi3071.
    KEGGihsa:3071.
    UCSCiuc001sgc.4. human. [P55160-1]

    Polymorphism databases

    DMDMi218512111.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58285 mRNA. Translation: AAA35964.1 . Different initiation.
    AK300783 mRNA. Translation: BAG62447.1 .
    AC025570 Genomic DNA. No translation available.
    AC068789 Genomic DNA. No translation available.
    AC079313 Genomic DNA. No translation available.
    CH471054 Genomic DNA. Translation: EAW96787.1 .
    BC093769 mRNA. Translation: AAH93769.1 .
    BC093771 mRNA. Translation: AAH93771.1 .
    CCDSi CCDS31813.1. [P55160-1 ]
    CCDS53799.1. [P55160-2 ]
    PIRi S36666.
    RefSeqi NP_001171905.1. NM_001184976.1. [P55160-2 ]
    NP_005328.2. NM_005337.4. [P55160-1 ]
    UniGenei Hs.182014.

    3D structure databases

    ProteinModelPortali P55160.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109321. 4 interactions.
    MINTi MINT-1711041.
    STRINGi 9606.ENSP00000293373.

    PTM databases

    PhosphoSitei P55160.

    Polymorphism databases

    DMDMi 218512111.

    Proteomic databases

    MaxQBi P55160.
    PaxDbi P55160.
    PRIDEi P55160.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000293373 ; ENSP00000293373 ; ENSG00000123338 . [P55160-1 ]
    ENST00000545638 ; ENSP00000445596 ; ENSG00000123338 . [P55160-2 ]
    GeneIDi 3071.
    KEGGi hsa:3071.
    UCSCi uc001sgc.4. human. [P55160-1 ]

    Organism-specific databases

    CTDi 3071.
    GeneCardsi GC12P054891.
    HGNCi HGNC:4862. NCKAP1L.
    HPAi HPA039490.
    HPA040772.
    MIMi 141180. gene.
    neXtProti NX_P55160.
    PharmGKBi PA29239.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG287735.
    HOGENOMi HOG000231880.
    HOVERGENi HBG006344.
    InParanoidi P55160.
    KOi K05750.
    OMAi AFMALSF.
    OrthoDBi EOG7TJ3H0.
    PhylomeDBi P55160.
    TreeFami TF313683.

    Miscellaneous databases

    GenomeRNAii 3071.
    NextBioi 12151.
    PROi P55160.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P55160.
    Bgeei P55160.
    CleanExi HS_NCKAP1L.
    Genevestigatori P55160.

    Family and domain databases

    InterProi IPR019137. Nck-associated_protein-1.
    [Graphical view ]
    Pfami PF09735. Nckap1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The HEM proteins: a novel family of tissue-specific transmembrane proteins expressed from invertebrates through mammals with an essential function in oogenesis."
      Baumgartner S., Martin D., Chiquet-Ehrismann R., Sutton J., Desai A., Huang I., Kato K., Hromas R.
      J. Mol. Biol. 251:41-49(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Blood.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    3. "The finished DNA sequence of human chromosome 12."
      Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
      , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
      Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Colon.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNCKPL_HUMAN
    AccessioniPrimary (citable) accession number: P55160
    Secondary accession number(s): B4DUT5, Q52LW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 16, 2008
    Last modified: October 1, 2014
    This is version 109 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3