P55160 (NCKPL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nck-associated protein 1-like

Alternative name(s):
Hematopoietic protein 1
Membrane-associated protein HEM-1

Gene names

Name:	NCKAP1L
Synonyms:	HEM1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1127 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell membrane; Single-pass membrane protein; Cytoplasmic side Probable.
Tissue specificity	Expressed only in cells of hematopoietic origin.
Sequence similarities	Belongs to the HEM-1/HEM-2 family.
Sequence caution	The sequence AAA35964.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	B cell homeostasis Inferred from sequence or structural similarity. Source: UniProtKB B cell receptor signaling pathway Inferred from mutant phenotype PubMed 17875758. Source: UniProtKB T cell homeostasis Inferred from sequence or structural similarity. Source: UniProtKB actin polymerization-dependent cell motility Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB cortical actin cytoskeleton organization Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB erythrocyte development Inferred from expression pattern Ref.1. Source: UniProtKB maintenance of cell polarity Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB myeloid cell homeostasis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of apoptotic process Inferred from mutant phenotype PubMed 17875758. Source: UniProtKB negative regulation of interleukin-17 production Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of interleukin-6 production Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of myosin-light-chain-phosphatase activity Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB neutrophil chemotaxis Inferred from direct assay PubMed 16417406. Source: UniProtKB positive regulation of B cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of B cell proliferation Inferred from mutant phenotype PubMed 17875758. Source: UniProtKB positive regulation of CD4-positive, alpha-beta T cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of CD8-positive, alpha-beta T cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of T cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of actin filament polymerization Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB positive regulation of cell adhesion mediated by integrin Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of erythrocyte differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of gamma-delta T cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of neutrophil chemotaxis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of phagocytosis, engulfment Inferred from sequence or structural similarity. Source: UniProtKB protein complex assembly Inferred from sequence or structural similarity. Source: UniProtKB response to drug Inferred from mutant phenotype PubMed 17875758. Source: UniProtKB
Cellular_component	SCAR complex Inferred from direct assay PubMed 16417406. Source: UniProtKB cytosol Inferred from direct assay PubMed 16417406. Source: UniProtKB integral to plasma membrane Non-traceable author statement PubMed 1932118. Source: UniProtKB
Molecular_function	Rac GTPase activator activity Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB protein complex binding Inferred from direct assay PubMed 16417406. Source: UniProtKB protein kinase activator activity Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P55160-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P55160-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1127	1127	Nck-associated protein 1-like		PRO_0000216175
Regions
	Transmembrane	996 – 1016	21	Helical; Potential
Natural variations
	Alternative sequence	1 – 50	50	Missing in isoform 2.		VSP_045191
	Natural variant	391	1	T → A. Corresponds to variant rs7311877 [ dbSNP | Ensembl ].		VAR_059316
	Natural variant	402	1	S → L. Corresponds to variant rs2270581 [ dbSNP | Ensembl ].		VAR_049344
Experimental info
	Sequence conflict	496	1	T → H in AAA35964. Ref.1
	Sequence conflict	768 – 769	2	NA → KP in AAA35964. Ref.1
	Sequence conflict	1060	1	L → V in AAA35964. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 16, 2008. Version 3. Checksum: 9389BAB163BAEA45	FASTA	1,127	128,153
	10 20 30 40 50 60 MSLTSAYQHK LAEKLTILND RGQGVLIRMY NIKKTCSDPK SKPPFLLEKS MEPSLKYINK 70 80 90 100 110 120 KFPNIDVRNS TQHLGPVHRE KAEIIRFLTN YYQSFVDVME FRDHVYELLN TIDACQCHFD 130 140 150 160 170 180 INLNFDFTRS YLDLIVTYTS VILLLSRIED RRILIGMYNC AHEMLHGHGD PSFARLGQMV 190 200 210 220 230 240 LEYDHPLKKL TEEFGPHTKA VSGALLSLHF LFVRRNQGAE QWRSAQLLSL ISNPPAMINP 250 260 270 280 290 300 ANSDTMACEY LSVEVMERWI IIGFLLCHGC LNSNSQCQKL WKLCLQGSLY ITLIREDVLQ 310 320 330 340 350 360 VHKVTEDLFS SLKGYGKRVA DIKESKEHVI ANSGQFHCQR RQFLRMAVKE LETVLADEPG 370 380 390 400 410 420 LLGPKALFAF MALSFIRDEV TWLVRHTENV TKTKTPEDYA DSSIAELLFL LEGIRSLVRR 430 440 450 460 470 480 HIKVIQQYHL QYLARFDALV LSDIIQNLSV CPEEESIIMS SFVSILSSLN LKQVDNGEKF 490 500 510 520 530 540 EFSGLRLDWF RLQAYTSVAK APLHLHENPD LAKVMNLIVF HSRMLDSVEK LLVETSDLST 550 560 570 580 590 600 FCFHLRIFEK MFAMTLEESA MLRYAIAFPL ICAHFVHCTH EMCPEEYPHL KNHGLHHCNS 610 620 630 640 650 660 FLEELAKQTS NCVLEICAEQ RNLSEQLLPK HCATTISKAK NKKTRKQRQT PRKGEPERDK 670 680 690 700 710 720 PGAESHRKNR SIVTNMDKLH LNLTELALTM NHVYSFSVFE HTIFPSEYLS SHLEARLNRA 730 740 750 760 770 780 IVWLAGYNAT TQEIVRPSEL LAGVKAYIGF IQSLAQFLGA DASRVIRNAL LQQTQPLDSC 790 800 810 820 830 840 GEQTITTLYT NWYLESLLRQ ASSGTIILSP AMQAFVSLPR EGEQNFSAEE FSDISEMRAL 850 860 870 880 890 900 AELLGPYGMK FLSENLMWHV TSQIVELKKL VVENMDILVQ IRSNFSKPDL MASLLPQLTG 910 920 930 940 950 960 AENVLKRMTI IGVILSFRAM AQEGLREVFS SHCPFLMGPI ECLKEFVTPD TDIKVTLSIF 970 980 990 1000 1010 1020 ELASAAGVGC DIDPALVAAI ANLKADTSSP EEEYKVACLL LIFLAVSLPL LATDPSSFYS 1030 1040 1050 1060 1070 1080 IEKDGYNNNI HCLTKAIIQV SAALFTLYNK NIETHLKEFL VVASVSLLQL GQETDKLKTR 1090 1100 1110 1120 NRESISLLMR LVVEESSFLT LDMLESCFPY VLLRNAYREV SRAFHLN
	Isoform 2 [UniParc]. Checksum: CE2F51F1F17E2C83	FASTA	1,077	122,478
	10 20 30 40 50 60 MEPSLKYINK KFPNIDVRNS TQHLGPVHRE KAEIIRFLTN YYQSFVDVME FRDHVYELLN 70 80 90 100 110 120 TIDACQCHFD INLNFDFTRS YLDLIVTYTS VILLLSRIED RRILIGMYNC AHEMLHGHGD 130 140 150 160 170 180 PSFARLGQMV LEYDHPLKKL TEEFGPHTKA VSGALLSLHF LFVRRNQGAE QWRSAQLLSL 190 200 210 220 230 240 ISNPPAMINP ANSDTMACEY LSVEVMERWI IIGFLLCHGC LNSNSQCQKL WKLCLQGSLY 250 260 270 280 290 300 ITLIREDVLQ VHKVTEDLFS SLKGYGKRVA DIKESKEHVI ANSGQFHCQR RQFLRMAVKE 310 320 330 340 350 360 LETVLADEPG LLGPKALFAF MALSFIRDEV TWLVRHTENV TKTKTPEDYA DSSIAELLFL 370 380 390 400 410 420 LEGIRSLVRR HIKVIQQYHL QYLARFDALV LSDIIQNLSV CPEEESIIMS SFVSILSSLN 430 440 450 460 470 480 LKQVDNGEKF EFSGLRLDWF RLQAYTSVAK APLHLHENPD LAKVMNLIVF HSRMLDSVEK 490 500 510 520 530 540 LLVETSDLST FCFHLRIFEK MFAMTLEESA MLRYAIAFPL ICAHFVHCTH EMCPEEYPHL 550 560 570 580 590 600 KNHGLHHCNS FLEELAKQTS NCVLEICAEQ RNLSEQLLPK HCATTISKAK NKKTRKQRQT 610 620 630 640 650 660 PRKGEPERDK PGAESHRKNR SIVTNMDKLH LNLTELALTM NHVYSFSVFE HTIFPSEYLS 670 680 690 700 710 720 SHLEARLNRA IVWLAGYNAT TQEIVRPSEL LAGVKAYIGF IQSLAQFLGA DASRVIRNAL 730 740 750 760 770 780 LQQTQPLDSC GEQTITTLYT NWYLESLLRQ ASSGTIILSP AMQAFVSLPR EGEQNFSAEE 790 800 810 820 830 840 FSDISEMRAL AELLGPYGMK FLSENLMWHV TSQIVELKKL VVENMDILVQ IRSNFSKPDL 850 860 870 880 890 900 MASLLPQLTG AENVLKRMTI IGVILSFRAM AQEGLREVFS SHCPFLMGPI ECLKEFVTPD 910 920 930 940 950 960 TDIKVTLSIF ELASAAGVGC DIDPALVAAI ANLKADTSSP EEEYKVACLL LIFLAVSLPL 970 980 990 1000 1010 1020 LATDPSSFYS IEKDGYNNNI HCLTKAIIQV SAALFTLYNK NIETHLKEFL VVASVSLLQL 1030 1040 1050 1060 1070 GQETDKLKTR NRESISLLMR LVVEESSFLT LDMLESCFPY VLLRNAYREV SRAFHLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The HEM proteins: a novel family of tissue-specific transmembrane proteins expressed from invertebrates through mammals with an essential function in oogenesis." Baumgartner S., Martin D., Chiquet-Ehrismann R., Sutton J., Desai A., Huang I., Kato K., Hromas R. J. Mol. Biol. 251:41-49(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Blood.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58285 mRNA. Translation: AAA35964.1. Different initiation. AK300783 mRNA. Translation: BAG62447.1. AC025570 Genomic DNA. No translation available. AC068789 Genomic DNA. No translation available. AC079313 Genomic DNA. No translation available. CH471054 Genomic DNA. Translation: EAW96787.1. BC093769 mRNA. Translation: AAH93769.1. BC093771 mRNA. Translation: AAH93771.1.
IPI	IPI00023407. IPI00793989.
PIR	S36666.
RefSeq	NP_001171905.1. NM_001184976.1. NP_005328.2. NM_005337.4.
UniGene	Hs.182014.
3D structure databases
ProteinModelPortal	P55160.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1711041.
STRING	9606.ENSP00000293373.
PTM databases
PhosphoSite	P55160.
Polymorphism databases
DMDM	218512111.
Proteomic databases
PaxDb	P55160.
PRIDE	P55160.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000293373; ENSP00000293373; ENSG00000123338. ENST00000545638; ENSP00000445596; ENSG00000123338.
GeneID	3071.
KEGG	hsa:3071.
UCSC	uc001sgc.4. human.
Organism-specific databases
CTD	3071.
GeneCards	GC12P054891.
HGNC	HGNC:4862. NCKAP1L.
HPA	HPA039490. HPA040772.
MIM	141180. gene.
neXtProt	NX_P55160.
PharmGKB	PA29239.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG287735.
HOGENOM	HOG000231880.
HOVERGEN	HBG006344.
InParanoid	P55160.
KO	K05750.
OMA	AFMALSF.
OrthoDB	EOG4894KS.
Gene expression databases
ArrayExpress	P55160.
Bgee	P55160.
CleanEx	HS_NCKAP1L.
Genevestigator	P55160.
GermOnline	ENSG00000123338. Homo sapiens.
Family and domain databases
InterPro	IPR019137. Nck-associated_protein-1. [Graphical view]
Pfam	PF09735. Nckap1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	3071.
NextBio	12151.
SOURCE	Search...

Entry information

Entry name

NCKPL_HUMAN

Accession

Primary (citable) accession number: P55160
Secondary accession number(s): B4DUT5, Q52LW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 16, 2008
Last modified:	April 3, 2013
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.