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P55160

- NCKPL_HUMAN

UniProt

P55160 - NCKPL_HUMAN

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Protein

Nck-associated protein 1-like

Gene

NCKAP1L

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. protein complex binding Source: UniProtKB
  2. protein kinase activator activity Source: UniProtKB
  3. Rac GTPase activator activity Source: UniProtKB

GO - Biological processi

  1. actin polymerization-dependent cell motility Source: UniProtKB
  2. B cell homeostasis Source: UniProtKB
  3. B cell receptor signaling pathway Source: UniProtKB
  4. chemotaxis Source: UniProtKB
  5. cortical actin cytoskeleton organization Source: UniProtKB
  6. erythrocyte development Source: UniProtKB
  7. maintenance of cell polarity Source: UniProtKB
  8. myeloid cell homeostasis Source: UniProtKB
  9. negative regulation of apoptotic process Source: UniProtKB
  10. negative regulation of interleukin-17 production Source: UniProtKB
  11. negative regulation of interleukin-6 production Source: UniProtKB
  12. negative regulation of myosin-light-chain-phosphatase activity Source: UniProtKB
  13. neutrophil chemotaxis Source: UniProtKB
  14. positive regulation of actin filament polymerization Source: UniProtKB
  15. positive regulation of B cell differentiation Source: UniProtKB
  16. positive regulation of B cell proliferation Source: UniProtKB
  17. positive regulation of CD4-positive, alpha-beta T cell differentiation Source: UniProtKB
  18. positive regulation of CD8-positive, alpha-beta T cell differentiation Source: UniProtKB
  19. positive regulation of cell adhesion mediated by integrin Source: UniProtKB
  20. positive regulation of erythrocyte differentiation Source: UniProtKB
  21. positive regulation of gamma-delta T cell differentiation Source: UniProtKB
  22. positive regulation of lymphocyte differentiation Source: UniProtKB
  23. positive regulation of neutrophil chemotaxis Source: UniProtKB
  24. positive regulation of phagocytosis, engulfment Source: UniProtKB
  25. positive regulation of phosphorylation Source: UniProtKB
  26. positive regulation of protein kinase activity Source: GOC
  27. positive regulation of Rac GTPase activity Source: GOC
  28. positive regulation of T cell proliferation Source: UniProtKB
  29. protein complex assembly Source: UniProtKB
  30. response to drug Source: UniProtKB
  31. T cell homeostasis Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Nck-associated protein 1-like
Alternative name(s):
Hematopoietic protein 1
Membrane-associated protein HEM-1
Gene namesi
Name:NCKAP1L
Synonyms:HEM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:4862. NCKAP1L.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei996 – 101621HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. extracellular vesicular exosome Source: UniProt
  3. integral component of plasma membrane Source: UniProtKB
  4. membrane Source: UniProtKB
  5. SCAR complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29239.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11271127Nck-associated protein 1-likePRO_0000216175Add
BLAST

Proteomic databases

MaxQBiP55160.
PaxDbiP55160.
PRIDEiP55160.

PTM databases

PhosphoSiteiP55160.

Expressioni

Tissue specificityi

Expressed only in cells of hematopoietic origin.

Gene expression databases

BgeeiP55160.
CleanExiHS_NCKAP1L.
ExpressionAtlasiP55160. baseline and differential.
GenevestigatoriP55160.

Organism-specific databases

HPAiHPA039490.
HPA040772.

Interactioni

Protein-protein interaction databases

BioGridi109321. 4 interactions.
MINTiMINT-1711041.
STRINGi9606.ENSP00000293373.

Structurei

3D structure databases

ProteinModelPortaliP55160.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HEM-1/HEM-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG287735.
GeneTreeiENSGT00390000016619.
HOGENOMiHOG000231880.
HOVERGENiHBG006344.
InParanoidiP55160.
KOiK05750.
OMAiAFMALSF.
OrthoDBiEOG7TJ3H0.
PhylomeDBiP55160.
TreeFamiTF313683.

Family and domain databases

InterProiIPR019137. Nck-associated_protein-1.
[Graphical view]
PfamiPF09735. Nckap1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P55160-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLTSAYQHK LAEKLTILND RGQGVLIRMY NIKKTCSDPK SKPPFLLEKS
60 70 80 90 100
MEPSLKYINK KFPNIDVRNS TQHLGPVHRE KAEIIRFLTN YYQSFVDVME
110 120 130 140 150
FRDHVYELLN TIDACQCHFD INLNFDFTRS YLDLIVTYTS VILLLSRIED
160 170 180 190 200
RRILIGMYNC AHEMLHGHGD PSFARLGQMV LEYDHPLKKL TEEFGPHTKA
210 220 230 240 250
VSGALLSLHF LFVRRNQGAE QWRSAQLLSL ISNPPAMINP ANSDTMACEY
260 270 280 290 300
LSVEVMERWI IIGFLLCHGC LNSNSQCQKL WKLCLQGSLY ITLIREDVLQ
310 320 330 340 350
VHKVTEDLFS SLKGYGKRVA DIKESKEHVI ANSGQFHCQR RQFLRMAVKE
360 370 380 390 400
LETVLADEPG LLGPKALFAF MALSFIRDEV TWLVRHTENV TKTKTPEDYA
410 420 430 440 450
DSSIAELLFL LEGIRSLVRR HIKVIQQYHL QYLARFDALV LSDIIQNLSV
460 470 480 490 500
CPEEESIIMS SFVSILSSLN LKQVDNGEKF EFSGLRLDWF RLQAYTSVAK
510 520 530 540 550
APLHLHENPD LAKVMNLIVF HSRMLDSVEK LLVETSDLST FCFHLRIFEK
560 570 580 590 600
MFAMTLEESA MLRYAIAFPL ICAHFVHCTH EMCPEEYPHL KNHGLHHCNS
610 620 630 640 650
FLEELAKQTS NCVLEICAEQ RNLSEQLLPK HCATTISKAK NKKTRKQRQT
660 670 680 690 700
PRKGEPERDK PGAESHRKNR SIVTNMDKLH LNLTELALTM NHVYSFSVFE
710 720 730 740 750
HTIFPSEYLS SHLEARLNRA IVWLAGYNAT TQEIVRPSEL LAGVKAYIGF
760 770 780 790 800
IQSLAQFLGA DASRVIRNAL LQQTQPLDSC GEQTITTLYT NWYLESLLRQ
810 820 830 840 850
ASSGTIILSP AMQAFVSLPR EGEQNFSAEE FSDISEMRAL AELLGPYGMK
860 870 880 890 900
FLSENLMWHV TSQIVELKKL VVENMDILVQ IRSNFSKPDL MASLLPQLTG
910 920 930 940 950
AENVLKRMTI IGVILSFRAM AQEGLREVFS SHCPFLMGPI ECLKEFVTPD
960 970 980 990 1000
TDIKVTLSIF ELASAAGVGC DIDPALVAAI ANLKADTSSP EEEYKVACLL
1010 1020 1030 1040 1050
LIFLAVSLPL LATDPSSFYS IEKDGYNNNI HCLTKAIIQV SAALFTLYNK
1060 1070 1080 1090 1100
NIETHLKEFL VVASVSLLQL GQETDKLKTR NRESISLLMR LVVEESSFLT
1110 1120
LDMLESCFPY VLLRNAYREV SRAFHLN
Length:1,127
Mass (Da):128,153
Last modified:December 16, 2008 - v3
Checksum:i9389BAB163BAEA45
GO
Isoform 2 (identifier: P55160-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.

Note: No experimental confirmation available.

Show »
Length:1,077
Mass (Da):122,478
Checksum:iCE2F51F1F17E2C83
GO

Sequence cautioni

The sequence AAA35964.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti496 – 4961T → H in AAA35964. (PubMed:7643388)Curated
Sequence conflicti768 – 7692NA → KP in AAA35964. (PubMed:7643388)Curated
Sequence conflicti1060 – 10601L → V in AAA35964. (PubMed:7643388)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti391 – 3911T → A.
Corresponds to variant rs7311877 [ dbSNP | Ensembl ].
VAR_059316
Natural varianti402 – 4021S → L.
Corresponds to variant rs2270581 [ dbSNP | Ensembl ].
VAR_049344

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5050Missing in isoform 2. 1 PublicationVSP_045191Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58285 mRNA. Translation: AAA35964.1. Different initiation.
AK300783 mRNA. Translation: BAG62447.1.
AC025570 Genomic DNA. No translation available.
AC068789 Genomic DNA. No translation available.
AC079313 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW96787.1.
BC093769 mRNA. Translation: AAH93769.1.
BC093771 mRNA. Translation: AAH93771.1.
CCDSiCCDS31813.1. [P55160-1]
CCDS53799.1. [P55160-2]
PIRiS36666.
RefSeqiNP_001171905.1. NM_001184976.1. [P55160-2]
NP_005328.2. NM_005337.4. [P55160-1]
UniGeneiHs.182014.

Genome annotation databases

EnsembliENST00000293373; ENSP00000293373; ENSG00000123338. [P55160-1]
ENST00000545638; ENSP00000445596; ENSG00000123338. [P55160-2]
GeneIDi3071.
KEGGihsa:3071.
UCSCiuc001sgc.4. human. [P55160-1]

Polymorphism databases

DMDMi218512111.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58285 mRNA. Translation: AAA35964.1 . Different initiation.
AK300783 mRNA. Translation: BAG62447.1 .
AC025570 Genomic DNA. No translation available.
AC068789 Genomic DNA. No translation available.
AC079313 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW96787.1 .
BC093769 mRNA. Translation: AAH93769.1 .
BC093771 mRNA. Translation: AAH93771.1 .
CCDSi CCDS31813.1. [P55160-1 ]
CCDS53799.1. [P55160-2 ]
PIRi S36666.
RefSeqi NP_001171905.1. NM_001184976.1. [P55160-2 ]
NP_005328.2. NM_005337.4. [P55160-1 ]
UniGenei Hs.182014.

3D structure databases

ProteinModelPortali P55160.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 109321. 4 interactions.
MINTi MINT-1711041.
STRINGi 9606.ENSP00000293373.

PTM databases

PhosphoSitei P55160.

Polymorphism databases

DMDMi 218512111.

Proteomic databases

MaxQBi P55160.
PaxDbi P55160.
PRIDEi P55160.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000293373 ; ENSP00000293373 ; ENSG00000123338 . [P55160-1 ]
ENST00000545638 ; ENSP00000445596 ; ENSG00000123338 . [P55160-2 ]
GeneIDi 3071.
KEGGi hsa:3071.
UCSCi uc001sgc.4. human. [P55160-1 ]

Organism-specific databases

CTDi 3071.
GeneCardsi GC12P054891.
HGNCi HGNC:4862. NCKAP1L.
HPAi HPA039490.
HPA040772.
MIMi 141180. gene.
neXtProti NX_P55160.
PharmGKBi PA29239.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG287735.
GeneTreei ENSGT00390000016619.
HOGENOMi HOG000231880.
HOVERGENi HBG006344.
InParanoidi P55160.
KOi K05750.
OMAi AFMALSF.
OrthoDBi EOG7TJ3H0.
PhylomeDBi P55160.
TreeFami TF313683.

Miscellaneous databases

GenomeRNAii 3071.
NextBioi 12151.
PROi P55160.
SOURCEi Search...

Gene expression databases

Bgeei P55160.
CleanExi HS_NCKAP1L.
ExpressionAtlasi P55160. baseline and differential.
Genevestigatori P55160.

Family and domain databases

InterProi IPR019137. Nck-associated_protein-1.
[Graphical view ]
Pfami PF09735. Nckap1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The HEM proteins: a novel family of tissue-specific transmembrane proteins expressed from invertebrates through mammals with an essential function in oogenesis."
    Baumgartner S., Martin D., Chiquet-Ehrismann R., Sutton J., Desai A., Huang I., Kato K., Hromas R.
    J. Mol. Biol. 251:41-49(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Blood.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Colon.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNCKPL_HUMAN
AccessioniPrimary (citable) accession number: P55160
Secondary accession number(s): B4DUT5, Q52LW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 16, 2008
Last modified: October 29, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3