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P55160 (NCKPL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nck-associated protein 1-like
Alternative name(s):
Hematopoietic protein 1
Membrane-associated protein HEM-1
Gene names
Name:NCKAP1L
Synonyms:HEM1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1127 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Cell membrane; Single-pass membrane protein; Cytoplasmic side Probable.

Tissue specificity

Expressed only in cells of hematopoietic origin.

Sequence similarities

Belongs to the HEM-1/HEM-2 family.

Sequence caution

The sequence AAA35964.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processB cell homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

B cell receptor signaling pathway

Inferred from mutant phenotype PubMed 17875758. Source: UniProtKB

T cell homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

actin polymerization-dependent cell motility

Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB

chemotaxis

Inferred from direct assay PubMed 16417406. Source: UniProtKB

cortical actin cytoskeleton organization

Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB

erythrocyte development

Inferred from expression pattern Ref.1. Source: UniProtKB

maintenance of cell polarity

Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB

myeloid cell homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 17875758. Source: UniProtKB

negative regulation of interleukin-17 production

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of interleukin-6 production

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of myosin-light-chain-phosphatase activity

Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB

neutrophil chemotaxis

Inferred from direct assay PubMed 16417406. Source: UniProtKB

positive regulation of B cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of B cell proliferation

Inferred from mutant phenotype PubMed 17875758. Source: UniProtKB

positive regulation of CD4-positive, alpha-beta T cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of CD8-positive, alpha-beta T cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of Rac GTPase activity

Inferred from mutant phenotype PubMed 16417406. Source: GOC

positive regulation of T cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of actin filament polymerization

Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB

positive regulation of cell adhesion mediated by integrin

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of erythrocyte differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of gamma-delta T cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of lymphocyte differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of neutrophil chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of phagocytosis, engulfment

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of phosphorylation

Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB

positive regulation of protein kinase activity

Inferred from mutant phenotype PubMed 16417406. Source: GOC

protein complex assembly

Inferred from sequence or structural similarity. Source: UniProtKB

response to drug

Inferred from mutant phenotype PubMed 17875758. Source: UniProtKB

   Cellular_componentSCAR complex

Inferred from direct assay PubMed 16417406. Source: UniProtKB

cytosol

Inferred from direct assay PubMed 16417406. Source: UniProtKB

extracellular vesicular exosome

Inferred from direct assay PubMed 20458337. Source: UniProt

integral component of plasma membrane

Non-traceable author statement PubMed 1932118. Source: UniProtKB

membrane

Traceable author statement PubMed 1932118. Source: ProtInc

   Molecular_functionRac GTPase activator activity

Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB

protein complex binding

Inferred from direct assay PubMed 16417406. Source: UniProtKB

protein kinase activator activity

Inferred from mutant phenotype PubMed 16417406. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P55160-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P55160-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11271127Nck-associated protein 1-like
PRO_0000216175

Regions

Transmembrane996 – 101621Helical; Potential

Natural variations

Alternative sequence1 – 5050Missing in isoform 2.
VSP_045191
Natural variant3911T → A.
Corresponds to variant rs7311877 [ dbSNP | Ensembl ].
VAR_059316
Natural variant4021S → L.
Corresponds to variant rs2270581 [ dbSNP | Ensembl ].
VAR_049344

Experimental info

Sequence conflict4961T → H in AAA35964. Ref.1
Sequence conflict768 – 7692NA → KP in AAA35964. Ref.1
Sequence conflict10601L → V in AAA35964. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 16, 2008. Version 3.
Checksum: 9389BAB163BAEA45

FASTA1,127128,153
        10         20         30         40         50         60 
MSLTSAYQHK LAEKLTILND RGQGVLIRMY NIKKTCSDPK SKPPFLLEKS MEPSLKYINK 

        70         80         90        100        110        120 
KFPNIDVRNS TQHLGPVHRE KAEIIRFLTN YYQSFVDVME FRDHVYELLN TIDACQCHFD 

       130        140        150        160        170        180 
INLNFDFTRS YLDLIVTYTS VILLLSRIED RRILIGMYNC AHEMLHGHGD PSFARLGQMV 

       190        200        210        220        230        240 
LEYDHPLKKL TEEFGPHTKA VSGALLSLHF LFVRRNQGAE QWRSAQLLSL ISNPPAMINP 

       250        260        270        280        290        300 
ANSDTMACEY LSVEVMERWI IIGFLLCHGC LNSNSQCQKL WKLCLQGSLY ITLIREDVLQ 

       310        320        330        340        350        360 
VHKVTEDLFS SLKGYGKRVA DIKESKEHVI ANSGQFHCQR RQFLRMAVKE LETVLADEPG 

       370        380        390        400        410        420 
LLGPKALFAF MALSFIRDEV TWLVRHTENV TKTKTPEDYA DSSIAELLFL LEGIRSLVRR 

       430        440        450        460        470        480 
HIKVIQQYHL QYLARFDALV LSDIIQNLSV CPEEESIIMS SFVSILSSLN LKQVDNGEKF 

       490        500        510        520        530        540 
EFSGLRLDWF RLQAYTSVAK APLHLHENPD LAKVMNLIVF HSRMLDSVEK LLVETSDLST 

       550        560        570        580        590        600 
FCFHLRIFEK MFAMTLEESA MLRYAIAFPL ICAHFVHCTH EMCPEEYPHL KNHGLHHCNS 

       610        620        630        640        650        660 
FLEELAKQTS NCVLEICAEQ RNLSEQLLPK HCATTISKAK NKKTRKQRQT PRKGEPERDK 

       670        680        690        700        710        720 
PGAESHRKNR SIVTNMDKLH LNLTELALTM NHVYSFSVFE HTIFPSEYLS SHLEARLNRA 

       730        740        750        760        770        780 
IVWLAGYNAT TQEIVRPSEL LAGVKAYIGF IQSLAQFLGA DASRVIRNAL LQQTQPLDSC 

       790        800        810        820        830        840 
GEQTITTLYT NWYLESLLRQ ASSGTIILSP AMQAFVSLPR EGEQNFSAEE FSDISEMRAL 

       850        860        870        880        890        900 
AELLGPYGMK FLSENLMWHV TSQIVELKKL VVENMDILVQ IRSNFSKPDL MASLLPQLTG 

       910        920        930        940        950        960 
AENVLKRMTI IGVILSFRAM AQEGLREVFS SHCPFLMGPI ECLKEFVTPD TDIKVTLSIF 

       970        980        990       1000       1010       1020 
ELASAAGVGC DIDPALVAAI ANLKADTSSP EEEYKVACLL LIFLAVSLPL LATDPSSFYS 

      1030       1040       1050       1060       1070       1080 
IEKDGYNNNI HCLTKAIIQV SAALFTLYNK NIETHLKEFL VVASVSLLQL GQETDKLKTR 

      1090       1100       1110       1120 
NRESISLLMR LVVEESSFLT LDMLESCFPY VLLRNAYREV SRAFHLN 

« Hide

Isoform 2 [UniParc].

Checksum: CE2F51F1F17E2C83
Show »

FASTA1,077122,478

References

« Hide 'large scale' references
[1]"The HEM proteins: a novel family of tissue-specific transmembrane proteins expressed from invertebrates through mammals with an essential function in oogenesis."
Baumgartner S., Martin D., Chiquet-Ehrismann R., Sutton J., Desai A., Huang I., Kato K., Hromas R.
J. Mol. Biol. 251:41-49(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Blood.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Colon.
[6]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M58285 mRNA. Translation: AAA35964.1. Different initiation.
AK300783 mRNA. Translation: BAG62447.1.
AC025570 Genomic DNA. No translation available.
AC068789 Genomic DNA. No translation available.
AC079313 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW96787.1.
BC093769 mRNA. Translation: AAH93769.1.
BC093771 mRNA. Translation: AAH93771.1.
PIRS36666.
RefSeqNP_001171905.1. NM_001184976.1.
NP_005328.2. NM_005337.4.
UniGeneHs.182014.

3D structure databases

ProteinModelPortalP55160.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109321. 4 interactions.
MINTMINT-1711041.
STRING9606.ENSP00000293373.

PTM databases

PhosphoSiteP55160.

Polymorphism databases

DMDM218512111.

Proteomic databases

PaxDbP55160.
PRIDEP55160.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000293373; ENSP00000293373; ENSG00000123338. [P55160-1]
ENST00000545638; ENSP00000445596; ENSG00000123338. [P55160-2]
GeneID3071.
KEGGhsa:3071.
UCSCuc001sgc.4. human. [P55160-1]

Organism-specific databases

CTD3071.
GeneCardsGC12P054891.
HGNCHGNC:4862. NCKAP1L.
HPAHPA039490.
HPA040772.
MIM141180. gene.
neXtProtNX_P55160.
PharmGKBPA29239.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG287735.
HOGENOMHOG000231880.
HOVERGENHBG006344.
InParanoidP55160.
KOK05750.
OMAAFMALSF.
OrthoDBEOG7TJ3H0.
PhylomeDBP55160.
TreeFamTF313683.

Gene expression databases

ArrayExpressP55160.
BgeeP55160.
CleanExHS_NCKAP1L.
GenevestigatorP55160.

Family and domain databases

InterProIPR019137. Nck-associated_protein-1.
[Graphical view]
PfamPF09735. Nckap1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi3071.
NextBio12151.
PROP55160.
SOURCESearch...

Entry information

Entry nameNCKPL_HUMAN
AccessionPrimary (citable) accession number: P55160
Secondary accession number(s): B4DUT5, Q52LW0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 16, 2008
Last modified: April 16, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM