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P55160

- NCKPL_HUMAN

UniProt

P55160 - NCKPL_HUMAN

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Protein
Nck-associated protein 1-like
Gene
NCKAP1L, HEM1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. Rac GTPase activator activity Source: UniProtKB
  2. protein binding Source: UniProtKB
  3. protein complex binding Source: UniProtKB
  4. protein kinase activator activity Source: UniProtKB

GO - Biological processi

  1. B cell homeostasis Source: UniProtKB
  2. B cell receptor signaling pathway Source: UniProtKB
  3. T cell homeostasis Source: UniProtKB
  4. actin polymerization-dependent cell motility Source: UniProtKB
  5. chemotaxis Source: UniProtKB
  6. cortical actin cytoskeleton organization Source: UniProtKB
  7. erythrocyte development Source: UniProtKB
  8. maintenance of cell polarity Source: UniProtKB
  9. myeloid cell homeostasis Source: UniProtKB
  10. negative regulation of apoptotic process Source: UniProtKB
  11. negative regulation of interleukin-17 production Source: UniProtKB
  12. negative regulation of interleukin-6 production Source: UniProtKB
  13. negative regulation of myosin-light-chain-phosphatase activity Source: UniProtKB
  14. neutrophil chemotaxis Source: UniProtKB
  15. positive regulation of B cell differentiation Source: UniProtKB
  16. positive regulation of B cell proliferation Source: UniProtKB
  17. positive regulation of CD4-positive, alpha-beta T cell differentiation Source: UniProtKB
  18. positive regulation of CD8-positive, alpha-beta T cell differentiation Source: UniProtKB
  19. positive regulation of Rac GTPase activity Source: GOC
  20. positive regulation of T cell proliferation Source: UniProtKB
  21. positive regulation of actin filament polymerization Source: UniProtKB
  22. positive regulation of cell adhesion mediated by integrin Source: UniProtKB
  23. positive regulation of erythrocyte differentiation Source: UniProtKB
  24. positive regulation of gamma-delta T cell differentiation Source: UniProtKB
  25. positive regulation of lymphocyte differentiation Source: UniProtKB
  26. positive regulation of neutrophil chemotaxis Source: UniProtKB
  27. positive regulation of phagocytosis, engulfment Source: UniProtKB
  28. positive regulation of phosphorylation Source: UniProtKB
  29. positive regulation of protein kinase activity Source: GOC
  30. protein complex assembly Source: UniProtKB
  31. response to drug Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Nck-associated protein 1-like
Alternative name(s):
Hematopoietic protein 1
Membrane-associated protein HEM-1
Gene namesi
Name:NCKAP1L
Synonyms:HEM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:4862. NCKAP1L.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei996 – 101621Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. SCAR complex Source: UniProtKB
  2. cytosol Source: UniProtKB
  3. extracellular vesicular exosome Source: UniProt
  4. integral component of plasma membrane Source: UniProtKB
  5. membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29239.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11271127Nck-associated protein 1-like
PRO_0000216175Add
BLAST

Proteomic databases

MaxQBiP55160.
PaxDbiP55160.
PRIDEiP55160.

PTM databases

PhosphoSiteiP55160.

Expressioni

Tissue specificityi

Expressed only in cells of hematopoietic origin.

Gene expression databases

ArrayExpressiP55160.
BgeeiP55160.
CleanExiHS_NCKAP1L.
GenevestigatoriP55160.

Organism-specific databases

HPAiHPA039490.
HPA040772.

Interactioni

Protein-protein interaction databases

BioGridi109321. 4 interactions.
MINTiMINT-1711041.
STRINGi9606.ENSP00000293373.

Structurei

3D structure databases

ProteinModelPortaliP55160.

Family & Domainsi

Sequence similaritiesi

Belongs to the HEM-1/HEM-2 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG287735.
HOGENOMiHOG000231880.
HOVERGENiHBG006344.
InParanoidiP55160.
KOiK05750.
OMAiAFMALSF.
OrthoDBiEOG7TJ3H0.
PhylomeDBiP55160.
TreeFamiTF313683.

Family and domain databases

InterProiIPR019137. Nck-associated_protein-1.
[Graphical view]
PfamiPF09735. Nckap1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P55160-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSLTSAYQHK LAEKLTILND RGQGVLIRMY NIKKTCSDPK SKPPFLLEKS     50
MEPSLKYINK KFPNIDVRNS TQHLGPVHRE KAEIIRFLTN YYQSFVDVME 100
FRDHVYELLN TIDACQCHFD INLNFDFTRS YLDLIVTYTS VILLLSRIED 150
RRILIGMYNC AHEMLHGHGD PSFARLGQMV LEYDHPLKKL TEEFGPHTKA 200
VSGALLSLHF LFVRRNQGAE QWRSAQLLSL ISNPPAMINP ANSDTMACEY 250
LSVEVMERWI IIGFLLCHGC LNSNSQCQKL WKLCLQGSLY ITLIREDVLQ 300
VHKVTEDLFS SLKGYGKRVA DIKESKEHVI ANSGQFHCQR RQFLRMAVKE 350
LETVLADEPG LLGPKALFAF MALSFIRDEV TWLVRHTENV TKTKTPEDYA 400
DSSIAELLFL LEGIRSLVRR HIKVIQQYHL QYLARFDALV LSDIIQNLSV 450
CPEEESIIMS SFVSILSSLN LKQVDNGEKF EFSGLRLDWF RLQAYTSVAK 500
APLHLHENPD LAKVMNLIVF HSRMLDSVEK LLVETSDLST FCFHLRIFEK 550
MFAMTLEESA MLRYAIAFPL ICAHFVHCTH EMCPEEYPHL KNHGLHHCNS 600
FLEELAKQTS NCVLEICAEQ RNLSEQLLPK HCATTISKAK NKKTRKQRQT 650
PRKGEPERDK PGAESHRKNR SIVTNMDKLH LNLTELALTM NHVYSFSVFE 700
HTIFPSEYLS SHLEARLNRA IVWLAGYNAT TQEIVRPSEL LAGVKAYIGF 750
IQSLAQFLGA DASRVIRNAL LQQTQPLDSC GEQTITTLYT NWYLESLLRQ 800
ASSGTIILSP AMQAFVSLPR EGEQNFSAEE FSDISEMRAL AELLGPYGMK 850
FLSENLMWHV TSQIVELKKL VVENMDILVQ IRSNFSKPDL MASLLPQLTG 900
AENVLKRMTI IGVILSFRAM AQEGLREVFS SHCPFLMGPI ECLKEFVTPD 950
TDIKVTLSIF ELASAAGVGC DIDPALVAAI ANLKADTSSP EEEYKVACLL 1000
LIFLAVSLPL LATDPSSFYS IEKDGYNNNI HCLTKAIIQV SAALFTLYNK 1050
NIETHLKEFL VVASVSLLQL GQETDKLKTR NRESISLLMR LVVEESSFLT 1100
LDMLESCFPY VLLRNAYREV SRAFHLN 1127
Length:1,127
Mass (Da):128,153
Last modified:December 16, 2008 - v3
Checksum:i9389BAB163BAEA45
GO
Isoform 2 (identifier: P55160-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.

Note: No experimental confirmation available.

Show »
Length:1,077
Mass (Da):122,478
Checksum:iCE2F51F1F17E2C83
GO

Sequence cautioni

The sequence AAA35964.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti391 – 3911T → A.
Corresponds to variant rs7311877 [ dbSNP | Ensembl ].
VAR_059316
Natural varianti402 – 4021S → L.
Corresponds to variant rs2270581 [ dbSNP | Ensembl ].
VAR_049344

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5050Missing in isoform 2.
VSP_045191Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti496 – 4961T → H in AAA35964. 1 Publication
Sequence conflicti768 – 7692NA → KP in AAA35964. 1 Publication
Sequence conflicti1060 – 10601L → V in AAA35964. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58285 mRNA. Translation: AAA35964.1. Different initiation.
AK300783 mRNA. Translation: BAG62447.1.
AC025570 Genomic DNA. No translation available.
AC068789 Genomic DNA. No translation available.
AC079313 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW96787.1.
BC093769 mRNA. Translation: AAH93769.1.
BC093771 mRNA. Translation: AAH93771.1.
CCDSiCCDS31813.1. [P55160-1]
CCDS53799.1. [P55160-2]
PIRiS36666.
RefSeqiNP_001171905.1. NM_001184976.1. [P55160-2]
NP_005328.2. NM_005337.4. [P55160-1]
UniGeneiHs.182014.

Genome annotation databases

EnsembliENST00000293373; ENSP00000293373; ENSG00000123338. [P55160-1]
ENST00000545638; ENSP00000445596; ENSG00000123338. [P55160-2]
GeneIDi3071.
KEGGihsa:3071.
UCSCiuc001sgc.4. human. [P55160-1]

Polymorphism databases

DMDMi218512111.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58285 mRNA. Translation: AAA35964.1 . Different initiation.
AK300783 mRNA. Translation: BAG62447.1 .
AC025570 Genomic DNA. No translation available.
AC068789 Genomic DNA. No translation available.
AC079313 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW96787.1 .
BC093769 mRNA. Translation: AAH93769.1 .
BC093771 mRNA. Translation: AAH93771.1 .
CCDSi CCDS31813.1. [P55160-1 ]
CCDS53799.1. [P55160-2 ]
PIRi S36666.
RefSeqi NP_001171905.1. NM_001184976.1. [P55160-2 ]
NP_005328.2. NM_005337.4. [P55160-1 ]
UniGenei Hs.182014.

3D structure databases

ProteinModelPortali P55160.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 109321. 4 interactions.
MINTi MINT-1711041.
STRINGi 9606.ENSP00000293373.

PTM databases

PhosphoSitei P55160.

Polymorphism databases

DMDMi 218512111.

Proteomic databases

MaxQBi P55160.
PaxDbi P55160.
PRIDEi P55160.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000293373 ; ENSP00000293373 ; ENSG00000123338 . [P55160-1 ]
ENST00000545638 ; ENSP00000445596 ; ENSG00000123338 . [P55160-2 ]
GeneIDi 3071.
KEGGi hsa:3071.
UCSCi uc001sgc.4. human. [P55160-1 ]

Organism-specific databases

CTDi 3071.
GeneCardsi GC12P054891.
HGNCi HGNC:4862. NCKAP1L.
HPAi HPA039490.
HPA040772.
MIMi 141180. gene.
neXtProti NX_P55160.
PharmGKBi PA29239.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG287735.
HOGENOMi HOG000231880.
HOVERGENi HBG006344.
InParanoidi P55160.
KOi K05750.
OMAi AFMALSF.
OrthoDBi EOG7TJ3H0.
PhylomeDBi P55160.
TreeFami TF313683.

Miscellaneous databases

GenomeRNAii 3071.
NextBioi 12151.
PROi P55160.
SOURCEi Search...

Gene expression databases

ArrayExpressi P55160.
Bgeei P55160.
CleanExi HS_NCKAP1L.
Genevestigatori P55160.

Family and domain databases

InterProi IPR019137. Nck-associated_protein-1.
[Graphical view ]
Pfami PF09735. Nckap1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The HEM proteins: a novel family of tissue-specific transmembrane proteins expressed from invertebrates through mammals with an essential function in oogenesis."
    Baumgartner S., Martin D., Chiquet-Ehrismann R., Sutton J., Desai A., Huang I., Kato K., Hromas R.
    J. Mol. Biol. 251:41-49(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Blood.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Colon.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNCKPL_HUMAN
AccessioniPrimary (citable) accession number: P55160
Secondary accession number(s): B4DUT5, Q52LW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 16, 2008
Last modified: July 9, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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