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Protein

Serum paraoxonase/arylesterase 1

Gene

Pon1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.By similarity

Catalytic activityi

A phenyl acetate + H2O = a phenol + acetate.By similarity
An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.By similarity
An N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine.By similarity

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Calcium 1; catalyticBy similarity
Metal bindingi54 – 541Calcium 2By similarity
Active sitei115 – 1151Proton acceptorBy similarity
Metal bindingi117 – 1171Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi168 – 1681Calcium 1; catalyticBy similarity
Metal bindingi169 – 1691Calcium 2By similarity
Metal bindingi224 – 2241Calcium 1; catalyticBy similarity
Metal bindingi269 – 2691Calcium 1; catalyticBy similarity
Metal bindingi270 – 2701Calcium 1; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.2. 5301.
3.1.8.1. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Serum paraoxonase/arylesterase 1 (EC:3.1.1.2By similarity, EC:3.1.1.81By similarity, EC:3.1.8.1By similarity)
Short name:
PON 1
Alternative name(s):
Aromatic esterase 1
Short name:
A-esterase 1
Serum aryldialkylphosphatase 1
Gene namesi
Name:Pon1
Synonyms:Pon
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi620062. Pon1.

Subcellular locationi

GO - Cellular componenti

  • blood microparticle Source: Ensembl
  • extracellular exosome Source: Ensembl
  • extracellular space Source: RGD
  • intracellular membrane-bounded organelle Source: RGD
  • spherical high-density lipoprotein particle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

HDL, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 355354Serum paraoxonase/arylesterase 1PRO_0000223284Add
BLAST
Signal peptidei2 – ?Not cleaved

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 353By similarity
Modified residuei166 – 1661PhosphoserineBy similarity
Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence analysis
Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence analysis
Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.By similarity
The signal sequence is not cleaved.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP55159.
PRIDEiP55159.

PTM databases

iPTMnetiP55159.
PhosphoSiteiP55159.

Expressioni

Tissue specificityi

Plasma. Associated with HDL.

Gene expression databases

BgeeiENSRNOG00000008902.
GenevisibleiP55159. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with CLU.By similarity

Protein-protein interaction databases

MINTiMINT-4593519.
STRINGi10116.ENSRNOP00000011823.

Structurei

3D structure databases

ProteinModelPortaliP55159.
SMRiP55159. Positions 16-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the paraoxonase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHDV. Eukaryota.
ENOG4111QK7. LUCA.
GeneTreeiENSGT00390000008932.
HOGENOMiHOG000252960.
HOVERGENiHBG003604.
InParanoidiP55159.
KOiK01045.
OMAiFFYDSEN.
OrthoDBiEOG091G0AV9.
PhylomeDBiP55159.
TreeFamiTF322436.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR002640. Arylesterase.
IPR008363. Paraoxonase1.
[Graphical view]
PfamiPF01731. Arylesterase. 1 hit.
[Graphical view]
PRINTSiPR01785. PARAOXONASE.
PR01786. PARAOXONASE1.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55159-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLLGLTLV GLVLALYKNH RSSYQTRLNA FREVTPVDLP NCTLVKGIEA
60 70 80 90 100
GAEDLEILPN GLTFFSTGLK YPGIKSFDPS KPGKILLMDL NEKEPAVSEL
110 120 130 140 150
AIMGNTLDMS SFNPHGISTF IDEDNTVYLL VVSHPDSSST VEVFKFQEEE
160 170 180 190 200
RSLLHLKTIT HELLPSINDI AAVGPESFYA TNDHYFADPY LRSWEMYLGL
210 220 230 240 250
SWSNVVYYSP DKVRVVADGF DFANGIGISL DGKYVYIAEL LAHKIHVYEK
260 270 280 290 300
HANWTLTPLK VLSFDTLVDN ISVDPVTGDL WVGCHPNGMR IFFYDSENPP
310 320 330 340 350
GSEVLRIQSI LSEDPKVTVV YAENGTVLQG TTVAAVYKGK LLIGTVFHRA

LCCDL
Length:355
Mass (Da):39,358
Last modified:October 5, 2010 - v3
Checksum:iFE2886875D01E52D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 267HRSSYQT → PLXDWYR AA sequence (Ref. 4) Curated
Sequence conflicti68 – 681G → F in AAB53441 (Ref. 5) Curated
Sequence conflicti354 – 3541D → Y in AAB53441 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473959 Genomic DNA. Translation: EDM15017.1.
BC091403 mRNA. Translation: AAH91403.1.
U94856 mRNA. Translation: AAB53441.1.
PIRiPT0088.
RefSeqiNP_114466.1. NM_032077.1.
UniGeneiRn.20732.

Genome annotation databases

EnsembliENSRNOT00000011823; ENSRNOP00000011823; ENSRNOG00000008902.
GeneIDi84024.
KEGGirno:84024.
UCSCiRGD:620062. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473959 Genomic DNA. Translation: EDM15017.1.
BC091403 mRNA. Translation: AAH91403.1.
U94856 mRNA. Translation: AAB53441.1.
PIRiPT0088.
RefSeqiNP_114466.1. NM_032077.1.
UniGeneiRn.20732.

3D structure databases

ProteinModelPortaliP55159.
SMRiP55159. Positions 16-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4593519.
STRINGi10116.ENSRNOP00000011823.

PTM databases

iPTMnetiP55159.
PhosphoSiteiP55159.

Proteomic databases

PaxDbiP55159.
PRIDEiP55159.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011823; ENSRNOP00000011823; ENSRNOG00000008902.
GeneIDi84024.
KEGGirno:84024.
UCSCiRGD:620062. rat.

Organism-specific databases

CTDi5444.
RGDi620062. Pon1.

Phylogenomic databases

eggNOGiENOG410IHDV. Eukaryota.
ENOG4111QK7. LUCA.
GeneTreeiENSGT00390000008932.
HOGENOMiHOG000252960.
HOVERGENiHBG003604.
InParanoidiP55159.
KOiK01045.
OMAiFFYDSEN.
OrthoDBiEOG091G0AV9.
PhylomeDBiP55159.
TreeFamiTF322436.

Enzyme and pathway databases

BRENDAi3.1.1.2. 5301.
3.1.8.1. 5301.

Miscellaneous databases

PROiP55159.

Gene expression databases

BgeeiENSRNOG00000008902.
GenevisibleiP55159. RN.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR002640. Arylesterase.
IPR008363. Paraoxonase1.
[Graphical view]
PfamiPF01731. Arylesterase. 1 hit.
[Graphical view]
PRINTSiPR01785. PARAOXONASE.
PR01786. PARAOXONASE1.
ProtoNetiSearch...

Entry informationi

Entry nameiPON1_RAT
AccessioniPrimary (citable) accession number: P55159
Secondary accession number(s): O08682, Q5BJN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 5, 2010
Last modified: September 7, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The preferential association of PON1 with HDL is mediated in part by its signal peptide, by binding phospholipids directly, rather than binding apo AI. The retained signal peptide may allow transfer of the protein between phospholipid surfaces.By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.