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Protein

Tyrosine-protein kinase receptor TYRO3

Gene

Tyro3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.4 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei540ATPPROSITE-ProRule annotation1
Active sitei645Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi514 – 522ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • apoptotic cell clearance Source: UniProtKB
  • forebrain cell migration Source: MGI
  • natural killer cell differentiation Source: MGI
  • negative regulation of inflammatory response Source: UniProtKB
  • negative regulation of innate immune response Source: UniProtKB
  • negative regulation of lymphocyte activation Source: MGI
  • negative regulation of neuron apoptotic process Source: MGI
  • negative regulation of toll-like receptor signaling pathway Source: UniProtKB
  • neuron cellular homeostasis Source: UniProtKB
  • ovulation cycle Source: MGI
  • platelet activation Source: MGI
  • platelet aggregation Source: UniProtKB
  • protein autophosphorylation Source: UniProtKB
  • protein kinase B signaling Source: UniProtKB
  • secretion by cell Source: MGI
  • spermatogenesis Source: UniProtKB
  • substrate adhesion-dependent cell spreading Source: MGI
  • vagina development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase receptor TYRO3 (EC:2.7.10.1)
Alternative name(s):
Etk2/tyro3
TK19-2
Tyrosine-protein kinase DTK
Tyrosine-protein kinase RSE
Tyrosine-protein kinase TIF
Gene namesi
Name:Tyro3
Synonyms:Dtk, Rse, Tif
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:104294. Tyro3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini31 – 419ExtracellularSequence analysisAdd BLAST389
Transmembranei420 – 440HelicalSequence analysisAdd BLAST21
Topological domaini441 – 880CytoplasmicSequence analysisAdd BLAST440

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

knockout mice are fertile, but male animals that lack all three receptors TYRO3, AXL and MERTK produce no mature sperm.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000002447931 – 880Tyrosine-protein kinase receptor TYRO3Add BLAST850

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi53N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi54 ↔ 107PROSITE-ProRule annotation
Glycosylationi75N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi150 ↔ 193PROSITE-ProRule annotation
Glycosylationi181N-linked (GlcNAc...)1 Publication1
Glycosylationi220N-linked (GlcNAc...)Sequence analysis1
Glycosylationi230N-linked (GlcNAc...)Sequence analysis1
Glycosylationi283N-linked (GlcNAc...)Sequence analysis1
Glycosylationi356N-linked (GlcNAc...)Sequence analysis1
Glycosylationi370N-linked (GlcNAc...)Sequence analysis1
Modified residuei456PhosphoserineCombined sources1
Modified residuei671Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei675Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei676Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei794Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei808PhosphoserineBy similarity1
Modified residuei859PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP55144.
PaxDbiP55144.
PRIDEiP55144.

PTM databases

iPTMnetiP55144.
PhosphoSitePlusiP55144.

Expressioni

Tissue specificityi

Abundant in the brain and lower levels in other tissues.

Gene expression databases

BgeeiENSMUSG00000027298.
CleanExiMM_TYRO3.
GenevisibleiP55144. MM.

Interactioni

Subunit structurei

Monomer and homodimer. Interacts (via N-terminus) with extracellular ligands TULP1 and GAS6. Interacts with PIK3R1; this interaction increases PI3-kinase activity (By similarity).By similarity

GO - Molecular functioni

  • phosphatidylinositol 3-kinase binding Source: UniProtKB
  • protein heterodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi204395. 1 interactor.
IntActiP55144. 1 interactor.
MINTiMINT-4138931.
STRINGi10090.ENSMUSP00000028763.

Structurei

Secondary structure

1880
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi509 – 517Combined sources9
Beta strandi520 – 526Combined sources7
Beta strandi536 – 541Combined sources6
Helixi550 – 562Combined sources13
Beta strandi575 – 578Combined sources4
Beta strandi589 – 594Combined sources6
Helixi601 – 609Combined sources9
Helixi619 – 638Combined sources20
Helixi648 – 650Combined sources3
Beta strandi651 – 653Combined sources3
Beta strandi659 – 661Combined sources3
Helixi686 – 688Combined sources3
Helixi691 – 696Combined sources6
Helixi701 – 716Combined sources16
Turni722 – 725Combined sources4
Helixi728 – 730Combined sources3
Helixi731 – 736Combined sources6
Helixi749 – 757Combined sources9
Helixi763 – 765Combined sources3
Helixi769 – 781Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QUPX-ray1.90A485-800[»]
4FEQX-ray2.20A485-800[»]
4FF8X-ray2.40A485-800[»]
ProteinModelPortaliP55144.
SMRiP55144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 118Ig-like C2-type 1Add BLAST88
Domaini129 – 209Ig-like C2-type 2Add BLAST81
Domaini217 – 310Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST94
Domaini315 – 406Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST92
Domaini508 – 785Protein kinasePROSITE-ProRule annotationAdd BLAST278

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.PROSITE-ProRule annotation
Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG6I. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00810000125384.
HOVERGENiHBG006346.
InParanoidiP55144.
KOiK05116.
OMAiGMEDPEM.
OrthoDBiEOG091G016X.
TreeFamiTF317402.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.40.10. 4 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF07679. I-set. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00060. FN3. 2 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49265. SSF49265. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: P55144-1) [UniParc]FASTAAdd to basket
Also known as: III

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALRRSMGWP GLRPLLLAGL ASLLLPGSAA AGLKLMGAPV KMTVSQGQPV
60 70 80 90 100
KLNCSVEGME DPDIHWMKDG TVVQNASQVS ISISEHSWIG LLSLKSVERS
110 120 130 140 150
DAGLYWCQVK DGEETKISQS VWLTVEGVPF FTVEPKDLAV PPNAPFQLSC
160 170 180 190 200
EAVGPPEPVT IYWWRGLTKV GGPAPSPSVL NVTGVTQRTE FSCEARNIKG
210 220 230 240 250
LATSRPAIVR LQAPPAAPFN TTVTTISSYN ASVAWVPGAD GLALLHSCTV
260 270 280 290 300
QVAHAPGEWE ALAVVVPVPP FTCLLRNLAP ATNYSLRVRC ANALGPSPYG
310 320 330 340 350
DWVPFQTKGL APARAPQNFH AIRTDSGLIL EWEEVIPEDP GEGPLGPYKL
360 370 380 390 400
SWVQENGTQD ELMVEGTRAN LTDWDPQKDL ILRVCASNAI GDGPWSQPLV
410 420 430 440 450
VSSHDHAGRQ GPPHSRTSWV PVVLGVLTAL ITAAALALIL LRKRRKETRF
460 470 480 490 500
GQAFDSVMAR GEPAVHFRAA RSFNRERPER IEATLDSLGI SDELKEKLED
510 520 530 540 550
VLIPEQQFTL GRMLGKGEFG SVREAQLKQE DGSFVKVAVK MLKADIIASS
560 570 580 590 600
DIEEFLREAA CMKEFDHPHV AKLVGVSLRS RAKGRLPIPM VILPFMKHGD
610 620 630 640 650
LHAFLLASRI GENPFNLPLQ TLVRFMVDIA CGMEYLSSRN FIHRDLAARN
660 670 680 690 700
CMLAEDMTVC VADFGLSRKI YSGDYYRQGC ASKLPVKWLA LESLADNLYT
710 720 730 740 750
VHSDVWAFGV TMWEIMTRGQ TPYAGIENAE IYNYLIGGNR LKQPPECMEE
760 770 780 790 800
VYDLMYQCWS ADPKQRPSFT CLRMELENIL GHLSVLSTSQ DPLYINIERA
810 820 830 840 850
EQPTESGSPE VHCGERSSSE AGDGSGVGAV GGIPSDSRYI FSPGGLSESP
860 870 880
GQLEQQPESP LNENQRLLLL QQGLLPHSSC
Length:880
Mass (Da):96,208
Last modified:July 27, 2011 - v2
Checksum:i2794316CB0034617
GO
Isoform 2 (identifier: P55144-2) [UniParc]FASTAAdd to basket
Also known as: II

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: MALRRSMGWPGLRPLLLAGLASLLLPGSAAA → MGCPAGDWKV...QGAGPAAPSG

Show »
Length:894
Mass (Da):97,343
Checksum:i4F45E58F5B466FEA
GO
Isoform 1 (identifier: P55144-3) [UniParc]FASTAAdd to basket
Also known as: I, B

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: MALRRSMGWPGLRPLLLAGLASLLLPGSAAA → MDDKLENTLGRWAGENGLSIGEYLAIK

Show »
Length:876
Mass (Da):96,042
Checksum:i5C7813084CC59D56
GO

Sequence cautioni

The sequence AAB26942 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA54995 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti630A → R in CAA54995 (PubMed:8058320).Curated1
Sequence conflicti637 – 639SSR → AIK in BAA19191 (PubMed:8108112).Curated3
Sequence conflicti637 – 639SSR → AIK in BAA19192 (PubMed:8108112).Curated3
Sequence conflicti811V → L in AAA19237 (PubMed:7511603).Curated1
Sequence conflicti811V → L in AAC52148 (PubMed:7857657).Curated1
Sequence conflicti811V → L in BAA19193 (PubMed:8108112).Curated1
Sequence conflicti811V → L in AAB26942 (PubMed:7723626).Curated1
Sequence conflicti811V → L in AAB26943 (PubMed:7723626).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0125481 – 31MALRR…GSAAA → MDDKLENTLGRWAGENGLSI GEYLAIK in isoform 1. 3 PublicationsAdd BLAST31
Alternative sequenceiVSP_0125491 – 31MALRR…GSAAA → MGCPAGDWKVFGEGGAWPGA CPGSEAGPPQRQRSGQGAGP AAPSG in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05683 mRNA. Translation: AAA19237.1.
U18933 mRNA. Translation: AAC52148.1.
X78103 mRNA. Translation: CAA54995.1. Different initiation.
U18342 mRNA. Translation: AAB26942.1. Different initiation.
U18343 mRNA. Translation: AAB26943.1.
AB000826 Genomic DNA. Translation: BAA19191.1.
AB000827 mRNA. Translation: BAA19192.1.
AB000828 mRNA. Translation: BAA19193.1.
AK141198 mRNA. Translation: BAE24581.1.
AL844896 Genomic DNA. Translation: CAM22049.1.
BC066058 mRNA. Translation: AAH66058.1.
BC082325 mRNA. Translation: AAH82325.1.
U23721, U23718 Genomic DNA. Translation: AAC52215.1.
U23721, U23719 Genomic DNA. Translation: AAC52216.1.
U23721, U23720 Genomic DNA. Translation: AAC52217.1.
CCDSiCCDS16611.1. [P55144-1]
CCDS71119.1. [P55144-3]
PIRiB53743.
I48862.
I49152.
RefSeqiNP_001277729.1. NM_001290800.1. [P55144-3]
NP_062265.2. NM_019392.2. [P55144-1]
XP_017172636.1. XM_017317147.1. [P55144-2]
UniGeneiMm.2901.
Mm.424496.

Genome annotation databases

EnsembliENSMUST00000028763; ENSMUSP00000028763; ENSMUSG00000027298. [P55144-1]
ENSMUST00000110783; ENSMUSP00000106410; ENSMUSG00000027298. [P55144-3]
GeneIDi22174.
KEGGimmu:22174.
UCSCiuc008lup.2. mouse. [P55144-3]
uc008luq.1. mouse. [P55144-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05683 mRNA. Translation: AAA19237.1.
U18933 mRNA. Translation: AAC52148.1.
X78103 mRNA. Translation: CAA54995.1. Different initiation.
U18342 mRNA. Translation: AAB26942.1. Different initiation.
U18343 mRNA. Translation: AAB26943.1.
AB000826 Genomic DNA. Translation: BAA19191.1.
AB000827 mRNA. Translation: BAA19192.1.
AB000828 mRNA. Translation: BAA19193.1.
AK141198 mRNA. Translation: BAE24581.1.
AL844896 Genomic DNA. Translation: CAM22049.1.
BC066058 mRNA. Translation: AAH66058.1.
BC082325 mRNA. Translation: AAH82325.1.
U23721, U23718 Genomic DNA. Translation: AAC52215.1.
U23721, U23719 Genomic DNA. Translation: AAC52216.1.
U23721, U23720 Genomic DNA. Translation: AAC52217.1.
CCDSiCCDS16611.1. [P55144-1]
CCDS71119.1. [P55144-3]
PIRiB53743.
I48862.
I49152.
RefSeqiNP_001277729.1. NM_001290800.1. [P55144-3]
NP_062265.2. NM_019392.2. [P55144-1]
XP_017172636.1. XM_017317147.1. [P55144-2]
UniGeneiMm.2901.
Mm.424496.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QUPX-ray1.90A485-800[»]
4FEQX-ray2.20A485-800[»]
4FF8X-ray2.40A485-800[»]
ProteinModelPortaliP55144.
SMRiP55144.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204395. 1 interactor.
IntActiP55144. 1 interactor.
MINTiMINT-4138931.
STRINGi10090.ENSMUSP00000028763.

PTM databases

iPTMnetiP55144.
PhosphoSitePlusiP55144.

Proteomic databases

MaxQBiP55144.
PaxDbiP55144.
PRIDEiP55144.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028763; ENSMUSP00000028763; ENSMUSG00000027298. [P55144-1]
ENSMUST00000110783; ENSMUSP00000106410; ENSMUSG00000027298. [P55144-3]
GeneIDi22174.
KEGGimmu:22174.
UCSCiuc008lup.2. mouse. [P55144-3]
uc008luq.1. mouse. [P55144-1]

Organism-specific databases

CTDi7301.
MGIiMGI:104294. Tyro3.

Phylogenomic databases

eggNOGiENOG410IG6I. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00810000125384.
HOVERGENiHBG006346.
InParanoidiP55144.
KOiK05116.
OMAiGMEDPEM.
OrthoDBiEOG091G016X.
TreeFamiTF317402.

Enzyme and pathway databases

BRENDAi2.7.10.1. 3474.

Miscellaneous databases

PROiP55144.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027298.
CleanExiMM_TYRO3.
GenevisibleiP55144. MM.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.40.10. 4 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF07679. I-set. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00060. FN3. 2 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49265. SSF49265. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYRO3_MOUSE
AccessioniPrimary (citable) accession number: P55144
Secondary accession number(s): O09070
, O09080, P70285, Q60752, Q62482, Q62483, Q62484, Q6NZM6, Q78E85, Q78E87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.