P55133 (RELA_PHOAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP pyrophosphokinase
EC=2.7.6.5

Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I

Gene names

Name:

relA

Organism

Photobacterium angustum (strain S14 / CCUG 15956) (Vibrio sp. (strain S14 / CCUG 15956))

Taxonomic identifier

314292 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium › Photobacterium angustum

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp. Plays also a role in the reductive division that occurs during the initial phase of nutrient starvation.
Catalytic activity	ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.
Disruption phenotype	Mutants remain rod shaped, and do not acquire the typical coccoid morphology usually seen during starvation. Ref.1
Sequence similarities	Belongs to the RelA/SpoT family. Contains 1 ACT domain.

Ontologies

Keywords
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	guanosine tetraphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP diphosphokinase activity Inferred from electronic annotation. Source: EC amino acid binding Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 744	744	GTP pyrophosphokinase		PRO_0000166566
Regions
	Domain	668 – 742	75	ACT

Sequences

	Sequence		Length	Mass (Da)
	P55133 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B7AC772C4D47A40C	FASTA	744	84,546
	10 20 30 40 50 60 MVAVRGAHLK ENTTFELVSW VESLRQDAKV SSRIEGTYQR CIELAAEQEN GPLLLWRGRE 70 80 90 100 110 120 LVEILVTLSM DADTLIAALL YPLVEGGCYS TDALKEEYSG TILHLVQGVE QMCAISQLKS 130 140 150 160 170 180 TAEETAQAAQ VDNIRRMLLS MVDDFRCVVI KLAERICNLR EVKDQPDEVR RAAAQECANI 190 200 210 220 230 240 YAPLANRLGI GQLKWEIEDY AFRYQHPDTY KQIAKQLSER RIDREDYITH FVDDLSDAMK 250 260 270 280 290 300 ASNIRAEVQG RPKHIYSIWR KMQKKSLEFD ELFDVRAVRI VAEELQDCYA ALGVVHTKYR 310 320 330 340 350 360 HLPKEFDDYV ANPKPNGYQS IHTVVLGPEG KTIEIQIRTK QMHEESELGV AAHWKYKEGT 370 380 390 400 410 420 ASGGAQSAYD EKINWLRKLL AWQEEMSDSG EMLDELRSQV FDDRVYAFTP KGDVVDLPSN 430 440 450 460 470 480 ATPLDFAYHI HSEVGHRCIG AKVEGRIVPF TYHLQMGDQV EIITQKEPNP SRDWLNPNLG 490 500 510 520 530 540 FVTSSRARAK VHAWFRKQDR DKNIIAGKEI LEAELVKIHA TLKDAQYYAA KRFNVKSPEE 550 560 570 580 590 600 LYAGIGSGDL RINQVINHIN ALVNKPTAEE EDQQLLEKLS EASNKQATSH KKPQRDAVVV 610 620 630 640 650 660 EGVDNLMTHL ARCCQPIPGD DIQGFVTQGR GISVHRMDCE QLEELRHHAP ERIIDTVWGG 670 680 690 700 710 720 GFVGNYTITV RVTASERNGL LKELTNTLMN EKVKVAGMKS RVDYKKQMSI MDFELELTDL 730 740 EVLGRVLKRI EQVKDVAEAK RLYG

References

[1]

"Stringent control during carbon starvation of marine Vibrio sp. strain S14: molecular cloning, nucleotide sequence, and deletion of the relA gene." Flaerdh K., Axberg T., Albertson N.H., Kjelleberg S. J. Bacteriol. 176:5949-5957(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U13769 Genomic DNA. Translation: AAA62208.1.
3D structure databases
ProteinModelPortal	P55133.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	31352187. VBIPhoAng36222_3707.
Enzyme and pathway databases
UniPathway	UPA00908; UER00884.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR026020. (p)ppGpp_Synthase. IPR002912. ACT_dom. IPR012675. Beta-grasp_dom. IPR004811. RelA/Spo_fam. IPR007685. RelA_SpoT. IPR004095. TGS. IPR012676. TGS-like. [Graphical view]
PANTHER	PTHR21262. PTHR21262. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF04607. RelA_SpoT. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
SMART	SM00954. RelA_SpoT. 1 hit. [Graphical view]
SUPFAM	SSF81271. TGS-like. 1 hit.
TIGRFAMs	TIGR00691. spoT_relA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RELA_PHOAS

Accession

Primary (citable) accession number: P55133

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program