P55098 (PEX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisome biogenesis factor 2

Alternative name(s):
Peroxin-2
Peroxisomal membrane protein 3
Peroxisome assembly factor 1
Short name=PAF-1

Gene names

Name:	Pex2
Synonyms:	Paf1, Pmp35, Pxmp3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Somewhat implicated in the biogenesis of peroxisomes.
Subcellular location	Peroxisome membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the pex2/pex10/pex12 family. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Peroxisome biogenesis
Cellular component	Membrane Peroxisome
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bile acid biosynthetic process Inferred from mutant phenotype PubMed 14673138. Source: MGI cholesterol homeostasis Inferred from mutant phenotype PubMed 14673138. Source: MGI neuron migration Inferred from mutant phenotype PubMed 12746876. Source: MGI peroxisome organization Inferred from electronic annotation. Source: UniProtKB-KW regulation of cholesterol biosynthetic process Inferred from mutant phenotype PubMed 14673138. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW peroxisomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Peroxisome biogenesis factor 2		PRO_0000056370
Regions
	Transmembrane	140 – 159	20	Helical; Potential
	Transmembrane	195 – 213	19	Helical; Potential
	Zinc finger	244 – 284	41	RING-type
Experimental info
	Sequence conflict	1 – 8	8	MAAREEST → MTGKEENM in AAB91465. Ref.2
	Sequence conflict	85	1	H → Y in AAB91465. Ref.2
	Sequence conflict	91 – 94	4	PNPV → LNLI in AAB91465. Ref.2
	Sequence conflict	101	1	N → T in AAB91465. Ref.2
	Sequence conflict	105	1	L → W in AAB91465. Ref.2
	Sequence conflict	114	1	R → K in AAB91465. Ref.2
	Sequence conflict	138	1	C → F in AAB91465. Ref.2
	Sequence conflict	142	1	V → L in AAB91465. Ref.2
	Sequence conflict	152	1	M → I in AAB91465. Ref.2
	Sequence conflict	225 – 227	3	TLC → IPL in AAB91465. Ref.2
	Sequence conflict	295	1	A → S in AAB91465. Ref.2
	Sequence conflict	298	1	Q → E in AAB91465. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P55098 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: FAFB6C8913F959F0	FASTA	305	34,732
	10 20 30 40 50 60 MAAREESTQS ANRVLRISQL DALELNKALE QLVWSQFTQC FHGFKPGLLA RFEPEVKAFL 70 80 90 100 110 120 WLFLWRFTIY SKNATVGQSV LNIQHKNDSS PNPVYQPPSK NQKLLYAVCT IGGRWLEERC 130 140 150 160 170 180 YDLFRNRHLA SFGKAKQCMN FVVGLLKLGE LMNFLIFLQK GKFATLTERL LGIHSVFCKP 190 200 210 220 230 240 QNMREVGFEY MNRELLWHGF AEFLIFLLPL INIQKLKAKL SSWCTLCTGA AGHDSTLGSS 250 260 270 280 290 300 GKECALCGEW PTMPHTIGCE HVFCYYCVKS SFLFDIYFTC PKCGTEVHSV QPLKAGIQMS EVNAL

References

[1]	"Structure and expression of mammalian peroxisome assembly factor-1 (PMP35) genes." Wilson G.N., Bryant D.D. Biochem. Med. Metab. Biol. 51:140-148(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Swiss Webster. Tissue: Liver.
[2]	"Targeted deletion of the PEX2 peroxisome assembly gene in mice provides a model for Zellweger syndrome, a human neuronal migration disorder." Faust P.L., Hatten M.E. J. Cell Biol. 139:1293-1305(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Cerebellum.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L27842 Genomic DNA. Translation: AAA21742.1. AF031128 mRNA. Translation: AAB91465.1.
IPI	IPI00135652.
PIR	I52362.
RefSeq	NP_001156773.1. NM_001163301.2. NP_001156774.1. NM_001163302.2. NP_001156777.1. NM_001163305.2. NP_001156778.1. NM_001163306.2. NP_001254643.1. NM_001267714.1. NP_001254644.1. NM_001267715.1. NP_033020.2. NM_008994.4.
UniGene	Mm.132336.
3D structure databases
ProteinModelPortal	P55098.
SMR	P55098. Positions 239-288.
ModBase	Search...
Protein-protein interaction databases
IntAct	P55098. 1 interaction.
STRING	10090.ENSMUSP00000059415.
PTM databases
PhosphoSite	P55098.
Proteomic databases
PaxDb	P55098.
PRIDE	P55098.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	19302.
KEGG	mmu:19302.
UCSC	uc008oob.2. mouse.
Organism-specific databases
CTD	5828.
MGI	MGI:107486. Pex2.
Phylogenomic databases
eggNOG	NOG245402.
HOGENOM	HOG000294074.
HOVERGEN	HBG000416.
InParanoid	P55098.
KO	K06664.
OrthoDB	EOG48SGV1.
Gene expression databases
CleanEx	MM_PEX2.
Genevestigator	P55098.
GermOnline	ENSMUSG00000040374. Mus musculus.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR006845. Pex_N. IPR018957. Znf_C3HC4_RING-type. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF04757. Pex2_Pex12. 1 hit. PF00097. zf-C3HC4. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	296257.
SOURCE	Search...

Entry information

Entry name

PEX2_MOUSE

Accession

Primary (citable) accession number: P55098
Secondary accession number(s): O35467

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program