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Protein

Cathepsin K

Gene

Ctsk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation (By similarity).By similarity

Catalytic activityi

Broad proteolytic activity. With small-molecule substrates and inhibitors, the major determinant of specificity is P2, which is preferably Leu, Met > Phe, and not Arg.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei139By similarity1
Active sitei276By similarity1
Active sitei296By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14811.
ReactomeiR-MMU-1442490. Collagen degradation.
R-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-1679131. Trafficking and processing of endosomal TLR.

Protein family/group databases

MEROPSiI29.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin K (EC:3.4.22.38)
Gene namesi
Name:Ctsk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:107823. Ctsk.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075277.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
PropeptideiPRO_000002630116 – 114Activation peptideAdd BLAST99
ChainiPRO_0000026302115 – 329Cathepsin KAdd BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi103N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi136 ↔ 177By similarity
Disulfide bondi170 ↔ 210By similarity
Glycosylationi213N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi269 ↔ 318By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP55097.
PeptideAtlasiP55097.
PRIDEiP55097.

PTM databases

iPTMnetiP55097.
PhosphoSitePlusiP55097.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028111.
CleanExiMM_CTSK.
ExpressionAtlasiP55097. baseline and differential.
GenevisibleiP55097. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiP55097. 1 interactor.
MINTiMINT-3089515.
STRINGi10090.ENSMUSP00000015664.

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni121 – 125Combined sources5
Helixi139 – 156Combined sources18
Helixi164 – 170Combined sources7
Helixi176 – 178Combined sources3
Helixi182 – 192Combined sources11
Beta strandi195 – 197Combined sources3
Turni198 – 200Combined sources3
Helixi214 – 216Combined sources3
Beta strandi217 – 219Combined sources3
Beta strandi224 – 226Combined sources3
Helixi232 – 241Combined sources10
Beta strandi244 – 249Combined sources6
Helixi254 – 257Combined sources4
Beta strandi261 – 264Combined sources4
Beta strandi271 – 273Combined sources3
Beta strandi276 – 286Combined sources11
Beta strandi289 – 295Combined sources7
Beta strandi307 – 311Combined sources5
Beta strandi313 – 316Combined sources4
Helixi317 – 319Combined sources3
Turni320 – 323Combined sources4
Beta strandi325 – 327Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5T6UX-ray2.90A115-329[»]
ProteinModelPortaliP55097.
SMRiP55097.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00760000118871.
HOGENOMiHOG000230774.
HOVERGENiHBG011513.
InParanoidiP55097.
KOiK01371.
OMAiKGNKHWI.
OrthoDBiEOG091G0AKT.
PhylomeDBiP55097.
TreeFamiTF313739.

Family and domain databases

InterProiView protein in InterPro
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR015644. Peptidase_C1A_cathepsin-K.
IPR013201. Prot_inhib_I29.
PANTHERiPTHR12411. PTHR12411. 1 hit.
PTHR12411:SF442. PTHR12411:SF442. 1 hit.
PfamiView protein in Pfam
PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
PRINTSiPR00705. PAPAIN.
SMARTiView protein in SMART
SM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
PROSITEiView protein in PROSITE
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWVFKFLLLP MVSFALSPEE MLDTQWELWK KTHQKQYNSK VDEISRRLIW
60 70 80 90 100
EKNLKQISAH NLEASLGVHT YELAMNHLGD MTSEEVVQKM TGLRIPPSRS
110 120 130 140 150
YSNDTLYTPE WEGRVPDSID YRKKGYVTPV KNQGQCGSCW AFSSAGALEG
160 170 180 190 200
QLKKKTGKLL ALSPQNLVDC VTENYGCGGG YMTTAFQYVQ QNGGIDSEDA
210 220 230 240 250
YPYVGQDESC MYNATAKAAK CRGYREIPVG NEKALKRAVA RVGPISVSID
260 270 280 290 300
ASLASFQFYS RGVYYDENCD RDNVNHAVLV VGYGTQKGSK HWIIKNSWGE
310 320
SWGNKGYALL ARNKNNACGI TNMASFPKM
Length:329
Mass (Da):36,889
Last modified:December 1, 2000 - v2
Checksum:i4F4B28F664925778
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3 – 6VFKF → GLKV in CAA64218 (PubMed:8814310).Curated4
Sequence conflicti201Y → F in CAA64218 (PubMed:8814310).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94444 mRNA. Translation: CAA64218.1.
AJ006033 Genomic DNA. Translation: CAA06825.1.
BC046320 mRNA. Translation: AAH46320.1.
CCDSiCCDS17615.1.
PIRiS74227.
RefSeqiNP_031828.2. NM_007802.4.
XP_006501037.1. XM_006500974.3.
UniGeneiMm.272085.

Genome annotation databases

EnsembliENSMUST00000015664; ENSMUSP00000015664; ENSMUSG00000028111.
GeneIDi13038.
KEGGimmu:13038.
UCSCiuc008qjy.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCATK_MOUSE
AccessioniPrimary (citable) accession number: P55097
Secondary accession number(s): O88718
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: June 7, 2017
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families