P55097 (CATK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cathepsin K
EC=3.4.22.38

Gene names

Name:

Ctsk

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation By similarity.
Catalytic activity	Broad proteolytic activity. With small-molecule substrates and inhibitors, the major determinant of specificity is P2, which is preferably Leu, Met > Phe, and not Arg.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bone resorption Inferred from electronic annotation. Source: Compara intramembranous ossification Inferred from electronic annotation. Source: Compara proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: Compara lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Propeptide	16 – 114	99	Activation peptide		PRO_0000026301
	Chain	115 – 329	215	Cathepsin K		PRO_0000026302
Sites
	Active site	139	1	By similarity
	Active site	276	1	By similarity
	Active site	296	1	By similarity
Amino acid modifications
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	213	1	N-linked (GlcNAc...) Potential
	Disulfide bond	136 ↔ 177		By similarity
	Disulfide bond	170 ↔ 210		By similarity
	Disulfide bond	269 ↔ 318		By similarity
Experimental info
	Sequence conflict	3 – 6	4	VFKF → GLKV in CAA64218. Ref.1
	Sequence conflict	201	1	Y → F in CAA64218. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P55097 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 4F4B28F664925778	FASTA	329	36,889
	10 20 30 40 50 60 MWVFKFLLLP MVSFALSPEE MLDTQWELWK KTHQKQYNSK VDEISRRLIW EKNLKQISAH 70 80 90 100 110 120 NLEASLGVHT YELAMNHLGD MTSEEVVQKM TGLRIPPSRS YSNDTLYTPE WEGRVPDSID 130 140 150 160 170 180 YRKKGYVTPV KNQGQCGSCW AFSSAGALEG QLKKKTGKLL ALSPQNLVDC VTENYGCGGG 190 200 210 220 230 240 YMTTAFQYVQ QNGGIDSEDA YPYVGQDESC MYNATAKAAK CRGYREIPVG NEKALKRAVA 250 260 270 280 290 300 RVGPISVSID ASLASFQFYS RGVYYDENCD RDNVNHAVLV VGYGTQKGSK HWIIKNSWGE 310 320 SWGNKGYALL ARNKNNACGI TNMASFPKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse cathepsin K: cDNA cloning and predominant expression of the gene in osteoclasts, and in some hypertrophying chondrocytes during mouse development." Rantakokko J.A., Aro H.T., Savontaus M., Vuorio E. FEBS Lett. 393:307-313(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Calvaria.
[2]	"Complete genomic structure of the mouse cathepsin K gene (Ctsk) and its localization next to the Arnt gene on mouse chromosome 3." Rantakokko J.A., Kiviranta R., Eerola R., Aro H.T., Vuorio E. Matrix Biol. 18:155-161(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 129/SvJ.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94444 mRNA. Translation: CAA64218.1. AJ006033 Genomic DNA. Translation: CAA06825.1. BC046320 mRNA. Translation: AAH46320.1.
IPI	IPI00316575.
PIR	S74227.
RefSeq	NP_031828.2. NM_007802.3.
UniGene	Mm.272085.
3D structure databases
ProteinModelPortal	P55097.
SMR	P55097. Positions 16-329.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-3089515.
Protein family/group databases
MEROPS	I29.007.
PTM databases
PhosphoSite	P55097.
Proteomic databases
PRIDE	P55097.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000015664; ENSMUSP00000015664; ENSMUSG00000028111.
GeneID	13038.
KEGG	mmu:13038.
Organism-specific databases
CTD	1513.
MGI	MGI:107823. Ctsk.
Phylogenomic databases
eggNOG	COG4870.
HOGENOM	HOG000230774.
HOVERGEN	HBG011513.
InParanoid	P55097.
KO	K01371.
OMA	LKVPPSH.
OrthoDB	EOG4SJ5FC.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14811.
Gene expression databases
ArrayExpress	P55097.
Bgee	P55097.
CleanEx	MM_CTSK.
Genevestigator	P55097.
GermOnline	ENSMUSG00000028111. Mus musculus.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR025660. Pept_his_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR015644. Peptidase_C1A_cathepsin-K. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. PTHR12411. 1 hit. PTHR12411:SF55. PTHR12411:SF55. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00848. Inhibitor_I29. 1 hit. SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1075277.
NextBio	282924.
SOURCE	Search...

Entry information

Entry name

CATK_MOUSE

Accession

Primary (citable) accession number: P55097
Secondary accession number(s): O88718

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 1, 2000
Last modified:	April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program