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Protein

Cathepsin K

Gene

Ctsk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation (By similarity).By similarity

Catalytic activityi

Broad proteolytic activity. With small-molecule substrates and inhibitors, the major determinant of specificity is P2, which is preferably Leu, Met > Phe, and not Arg.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei139By similarity1
Active sitei276By similarity1
Active sitei296By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14811
ReactomeiR-MMU-1442490 Collagen degradation
R-MMU-1474228 Degradation of the extracellular matrix
R-MMU-1592389 Activation of Matrix Metalloproteinases
R-MMU-1679131 Trafficking and processing of endosomal TLR
R-MMU-2132295 MHC class II antigen presentation
R-MMU-8939242 RUNX1 regulates transcription of genes involved in differentiation of keratinocytes

Protein family/group databases

MEROPSiI29.007

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin K (EC:3.4.22.38)
Gene namesi
Name:Ctsk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:107823 Ctsk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075277

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
PropeptideiPRO_000002630116 – 114Activation peptideAdd BLAST99
ChainiPRO_0000026302115 – 329Cathepsin KAdd BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi103N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi136 ↔ 177By similarity
Disulfide bondi170 ↔ 210By similarity
Glycosylationi213N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi269 ↔ 318By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP55097
PeptideAtlasiP55097
PRIDEiP55097

PTM databases

iPTMnetiP55097
PhosphoSitePlusiP55097

Expressioni

Gene expression databases

BgeeiENSMUSG00000028111
CleanExiMM_CTSK
ExpressionAtlasiP55097 baseline and differential
GenevisibleiP55097 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiP55097 1 interactor.
MINTiP55097
STRINGi10090.ENSMUSP00000015664

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni121 – 125Combined sources5
Helixi139 – 156Combined sources18
Helixi164 – 170Combined sources7
Helixi176 – 178Combined sources3
Helixi182 – 192Combined sources11
Beta strandi195 – 197Combined sources3
Turni198 – 200Combined sources3
Helixi214 – 216Combined sources3
Beta strandi217 – 219Combined sources3
Beta strandi224 – 226Combined sources3
Helixi232 – 241Combined sources10
Beta strandi244 – 249Combined sources6
Helixi254 – 257Combined sources4
Beta strandi261 – 264Combined sources4
Beta strandi271 – 273Combined sources3
Beta strandi276 – 286Combined sources11
Beta strandi289 – 295Combined sources7
Beta strandi307 – 311Combined sources5
Beta strandi313 – 316Combined sources4
Helixi317 – 319Combined sources3
Turni320 – 323Combined sources4
Beta strandi325 – 327Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5T6UX-ray2.90A115-329[»]
ProteinModelPortaliP55097
SMRiP55097
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543 Eukaryota
COG4870 LUCA
GeneTreeiENSGT00900000140823
HOGENOMiHOG000230774
HOVERGENiHBG011513
InParanoidiP55097
KOiK01371
OMAiKGNKHWI
OrthoDBiEOG091G0AKT
PhylomeDBiP55097
TreeFamiTF313739

Family and domain databases

InterProiView protein in InterPro
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR015644 Peptidase_C1A_cathepsin-K
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PTHR12411:SF55 PTHR12411:SF55, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
PROSITEiView protein in PROSITE
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWVFKFLLLP MVSFALSPEE MLDTQWELWK KTHQKQYNSK VDEISRRLIW
60 70 80 90 100
EKNLKQISAH NLEASLGVHT YELAMNHLGD MTSEEVVQKM TGLRIPPSRS
110 120 130 140 150
YSNDTLYTPE WEGRVPDSID YRKKGYVTPV KNQGQCGSCW AFSSAGALEG
160 170 180 190 200
QLKKKTGKLL ALSPQNLVDC VTENYGCGGG YMTTAFQYVQ QNGGIDSEDA
210 220 230 240 250
YPYVGQDESC MYNATAKAAK CRGYREIPVG NEKALKRAVA RVGPISVSID
260 270 280 290 300
ASLASFQFYS RGVYYDENCD RDNVNHAVLV VGYGTQKGSK HWIIKNSWGE
310 320
SWGNKGYALL ARNKNNACGI TNMASFPKM
Length:329
Mass (Da):36,889
Last modified:December 1, 2000 - v2
Checksum:i4F4B28F664925778
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3 – 6VFKF → GLKV in CAA64218 (PubMed:8814310).Curated4
Sequence conflicti201Y → F in CAA64218 (PubMed:8814310).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94444 mRNA Translation: CAA64218.1
AJ006033 Genomic DNA Translation: CAA06825.1
BC046320 mRNA Translation: AAH46320.1
CCDSiCCDS17615.1
PIRiS74227
RefSeqiNP_031828.2, NM_007802.4
XP_006501037.1, XM_006500974.3
UniGeneiMm.272085

Genome annotation databases

EnsembliENSMUST00000015664; ENSMUSP00000015664; ENSMUSG00000028111
GeneIDi13038
KEGGimmu:13038
UCSCiuc008qjy.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCATK_MOUSE
AccessioniPrimary (citable) accession number: P55097
Secondary accession number(s): O88718
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: February 28, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome