Reviewed, UniProtKB/Swiss-Prot P55091 (CTRC_RAT)

Names and origin

Protein names

Recommended name:
    Chymotrypsin-C
    EC=3.4.21.2
Alternative name(s):
    Caldecrin
    Serum calcium-decreasing factor

Gene names

Name:

Ctrc

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has chymotrypsin-type protease activity and hypocalcemic activity.
Catalytic activity	Preferential cleavage: Leu-|-Xaa, Tyr-|-Xaa, Phe-|-Xaa, Met-|-Xaa, Trp-|-Xaa, Gln-|-Xaa, Asn-|-Xaa.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.
Caution	Was originally (Ref.2) thought to be elastase IV.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 29	13	Activation peptide		PRO_0000027715
	Chain	30 – 268	239	Chymotrypsin-C		PRO_0000027716
Regions
	Domain	30 – 267	238	Peptidase S1
Sites
	Active site	74	1	Charge relay system By similarity
	Active site	121	1	Charge relay system By similarity
	Active site	216	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	25	1	N-linked (GlcNAc...) Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Disulfide bond	17 ↔ 141		By similarity
	Disulfide bond	59 ↔ 75		By similarity
	Disulfide bond	155 ↔ 222		By similarity
	Disulfide bond	186 ↔ 202		By similarity
	Disulfide bond	212 ↔ 243		By similarity
Experimental info
	Sequence conflict	42	1	P → A in CAA41753. Ref.2
	Sequence conflict	96 – 120	25	EEGSV…LFLWN → AEAPCTLRWTPSTSMRSGTD SSCGT in CAA41753. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P55091-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 33B67AF34D0F8583	FASTA	268	29,374
	10 20 30 40 50 60 MLGITVLAAI LACASCCGNP AFPPNLSTRV VGGEDAVPNS WPWQVSLQYL KDDTWRHTCG 70 80 90 100 110 120 GSLITTSHVL TAAHCINKDF TYRVGLGKYN LTVEDEEGSV YAEVDTIYVH EKWNRLFLWN 130 140 150 160 170 180 DIAIIKLAEP VELSNTIQVA CIPEEGSLLP QDYPCYVTGW GRLWTNGPIA EVLQQGLQPI 190 200 210 220 230 240 VSHATCSRLD WWFIKVRKTM VCAGGDGVIS ACNGDSGGPL NCQAEDGSWQ VHGIVSFGSS 250 260 SGCNVHKKPV VFTRVSAYND WINEKIQL

References

[1]	"Molecular cloning and expression of serum calcium-decreasing factor (caldecrin)." Tomomura A., Tomomura M., Fukushige T., Akiyama M., Kubota N., Kumaki K., Nishii Y., Noikura T., Saheki T. J. Biol. Chem. 270:30315-30321(1995) [PubMed: 8530454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Pancreas.
[2]	"Identification of cDNAs encoding two novel rat pancreatic serine proteases." Kang J., Wiegand U., Mueller-Hill B. Gene 110:181-187(1992) [PubMed: 1537555] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.
[3]	"Caldecrin is a novel-type serine protease expressed in pancreas, but its homologue, elastase IV, is an artifact during cloning derived from caldecrin gene." Yoshino-Yasuda I., Kobayashi K., Akiyama M., Itoh H., Tomomura A., Saheki T. J. Biochem. 123:546-554(1998) [PubMed: 9538241] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S80379 mRNA. Translation: AAB35830.1. X59014 mRNA. Translation: CAA41753.1.
IPI	IPI00213599.
PIR	JQ1473.
RefSeq	NP_001071117.1.
UniGene	Rn.19214
3D structure databases
HSSP	HSSP built from PDB template 1PYT based on UniProtKB P05805.
SMR	P55091. Positions 17-268.
ModBase	Search...
Protein family/group databases
MEROPS	S01.157.
Proteomic databases
PRIDE	P55091.
Genome annotation databases
Ensembl	ENSRNOG00000013745. Rattus norvegicus. [Contig view]
GeneID	362653.
KEGG	rno:362653.
Organism-specific databases
RGD	1308379. Ctrc.
Phylogenomic databases
HOVERGEN	P55091.
OMA	P55091. WNDIAII.
Enzyme and pathway databases
BRENDA	3.4.21.2. 248.
Gene expression databases
ArrayExpress	P55091.
GermOnline	ENSRNOG00000013745. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	680758.

Entry information

Entry name

CTRC_RAT

Accession

Primary (citable) accession number: P55091
Secondary accession number(s): Q63188

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 5, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)