Aquaporin-4 - Mus musculus (Mouse)

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P55088 (AQP4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Aquaporin-4
Short name=AQP-4

Alternative name(s):
Mercurial-insensitive water channel
Short name=MIWC
WCH4

Gene names

Name:

Aqp4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
Subunit structure	Homotetramer By similarity. Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Post-translational modification	Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40% By similarity.
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon dioxide transport Inferred from electronic annotation. Source: Compara cellular response to estradiol stimulus Inferred from electronic annotation. Source: Compara cellular response to interferon-gamma Inferred from electronic annotation. Source: Compara female pregnancy Inferred from electronic annotation. Source: Compara hyperosmotic salinity response Inferred from electronic annotation. Source: Compara protein homooligomerization Inferred from electronic annotation. Source: Compara renal water absorption Inferred from mutant phenotype PubMed 10662735 PubMed 10795927. Source: MGI response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara response to radiation Inferred from electronic annotation. Source: Compara sensory perception of sound Inferred from mutant phenotype PubMed 11406631. Source: MGI
Cellular_component	T-tubule Inferred from electronic annotation. Source: Compara basolateral plasma membrane Inferred from direct assay PubMed 10021464 PubMed 10662735 PubMed 21178974. Source: MGI cell-cell junction Inferred from direct assay PubMed 18718702. Source: UniProtKB cytoplasm Inferred from direct assay PubMed 18718702. Source: UniProtKB external side of plasma membrane Inferred from electronic annotation. Source: Compara integral to plasma membrane Traceable author statement PubMed 11066060. Source: MGI protein complex Inferred from electronic annotation. Source: Compara
Molecular_function	porin activity Inferred from electronic annotation. Source: Compara water channel activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: P55088-1) Also known as: MIWC2; AQP4-M1; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: P55088-2) Also known as: MIWC1; AQP4-M21; The sequence of this isoform differs from the canonical sequence as follows: 1-22: Missing.

Isoform 3 (identifier: P55088-3) Also known as: MIWC3; The sequence of this isoform differs from the canonical sequence as follows: 1-11: MSDGAAARRWG → MVHGFGCFVFFFLISLSSLWASEDSTCNSTLPLCHLATTLDCC

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 323

323

Aquaporin-4

PRO_0000063949

Regions

Topological domain

1 – 36

Cytoplasmic Potential

Transmembrane

37 – 58

Helical; Potential

Topological domain

59 – 64

Extracellular Potential

Transmembrane

65 – 85

Helical; Potential

Topological domain

86 – 115

Cytoplasmic Potential

Transmembrane

116 – 136

Helical; Potential

Topological domain

137 – 155

Extracellular Potential

Transmembrane

156 – 176

Helical; Potential

Topological domain

177 – 184

Cytoplasmic Potential

Transmembrane

185 – 205

Helical; Potential

Topological domain

206 – 231

Extracellular Potential

Transmembrane

232 – 252

Helical; Potential

Topological domain

253 – 323

Cytoplasmic Potential

Motif

97 – 99

NPA 1

Motif

213 – 215

NPA 2

Amino acid modifications

Modified residue

111

Phosphoserine; by PKG By similarity

Modified residue

180

Phosphoserine; by PKC By similarity

Modified residue

285

Phosphoserine By similarity

Modified residue

321

Phosphoserine By similarity

Glycosylation

153

N-linked (GlcNAc...) Potential

Glycosylation

206

N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence

1 – 22

Missing in isoform 1.

VSP_003233

Alternative sequence

1 – 11

MSDGAAARRWG → MVHGFGCFVFFFLISLSSLW ASEDSTCNSTLPLCHLATTL DCC in isoform 3.

VSP_003234

Natural variant

G → R. Ref.3

Experimental info

Sequence conflict

A → S in AAB41571. Ref.1

Sequence conflict

Missing in AAB41569. Ref.1

Sequence conflict

Missing in AAB41570. Ref.1

Sequence conflict

F → L in AAB41569. Ref.1

Sequence conflict

F → L in AAB41570. Ref.1

Sequence conflict

174

I → V in AAB41569. Ref.1

Sequence conflict

174

I → V in AAB41570. Ref.1

Sequence conflict

313

K → R in AAB41569. Ref.1

Sequence conflict

313

K → R in AAB41570. Ref.1

Sequences

Sequence		Length	Mass (Da)
Isoform 2 (MIWC2) (AQP4-M1) [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 9AC638A3C3F412E2 Show »	FASTA	323	34,436
10 20 30 40 50 60 MSDGAAARRW GKCGHSCSRE SIMVAFKGVW TQAFWKAVSA EFLATLIFVL LGVGSTINWG 70 80 90 100 110 120 GSENPLPVDM VLISLCFGLS IATMVQCFGH ISGGHINPAV TVAMVCTRKI SIAKSVFYII 130 140 150 160 170 180 AQCLGAIIGA GILYLVTPPS VVGGLGVTTV HGNLTAGHGL LVELIITFQL VFTIFASCDS 190 200 210 220 230 240 KRTDVTGSIA LAIGFSVAIG HLFAINYTGA SMNPARSFGP AVIMGNWANH WIYWVGPIMG 250 260 270 280 290 300 AVLAGALYEY VFCPDVELKR RLKEAFSKAA QQTKGSYMEV EDNRSQVETE DLILKPGVVH 310 320 VIDIDRGEEK KGKDSSGEVL SSV « Hide
Isoform 1 (MIWC1) (AQP4-M21) [UniParc]. Checksum: E2ADDA3C8E0A92A2 Show »	FASTA	301	32,088
10 20 30 40 50 60 MVAFKGVWTQ AFWKAVSAEF LATLIFVLLG VGSTINWGGS ENPLPVDMVL ISLCFGLSIA 70 80 90 100 110 120 TMVQCFGHIS GGHINPAVTV AMVCTRKISI AKSVFYIIAQ CLGAIIGAGI LYLVTPPSVV 130 140 150 160 170 180 GGLGVTTVHG NLTAGHGLLV ELIITFQLVF TIFASCDSKR TDVTGSIALA IGFSVAIGHL 190 200 210 220 230 240 FAINYTGASM NPARSFGPAV IMGNWANHWI YWVGPIMGAV LAGALYEYVF CPDVELKRRL 250 260 270 280 290 300 KEAFSKAAQQ TKGSYMEVED NRSQVETEDL ILKPGVVHVI DIDRGEEKKG KDSSGEVLSS V « Hide
Isoform 3 (MIWC3) [UniParc]. Checksum: CDA0A8D89A1AC74A Show »	FASTA	355	37,977
10 20 30 40 50 60 MVHGFGCFVF FFLISLSSLW ASEDSTCNST LPLCHLATTL DCCKCGHSCS RESIMVAFKG 70 80 90 100 110 120 VWTQAFWKAV SAEFLATLIF VLLGVGSTIN WGGSENPLPV DMVLISLCFG LSIATMVQCF 130 140 150 160 170 180 GHISGGHINP AVTVAMVCTR KISIAKSVFY IIAQCLGAII GAGILYLVTP PSVVGGLGVT 190 200 210 220 230 240 TVHGNLTAGH GLLVELIITF QLVFTIFASC DSKRTDVTGS IALAIGFSVA IGHLFAINYT 250 260 270 280 290 300 GASMNPARSF GPAVIMGNWA NHWIYWVGPI MGAVLAGALY EYVFCPDVEL KRRLKEAFSK 310 320 330 340 350 AAQQTKGSYM EVEDNRSQVE TEDLILKPGV VHVIDIDRGE EKKGKDSSGE VLSSV « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene structure, cDNA cloning, and expression of a mouse mercurial-insensitive water channel." Ma T., Yang B., Verkman A.S. Genomics 33:382-388(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3). Strain: C57BL/6. Tissue: Brain.
[2]	"Cloning and chromosomal localization of mouse aquaporin 4: exclusion of a candidate mutant phenotype, ataxia." Turtzo L.C., Lee M.D., Lu M., Smith B.L., Copeland N.G., Gilbert D.J., Jenkins N.A., Agre P. Genomics 41:267-270(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Cerebellum.
[3]	"Identification of a new form of AQP4 mRNA that is developmentally expressed in mouse brain." Zelenin S., Gunnarson E., Alikina T., Bondar A., Aperia A. Pediatr. Res. 48:335-339(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT ARG-4.
[4]	"Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)." Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U48398 mRNA. Translation: AAB41569.1. U48397 mRNA. Translation: AAB41568.1. U33012 mRNA. Translation: AAA84923.1. U48400 Genomic DNA. Translation: AAB41571.1. U48399 mRNA. Translation: AAB41570.1. U88623 mRNA. Translation: AAC53155.1. AF469168 mRNA. Translation: AAL73545.1. AF469169 mRNA. Translation: AAL73546.1. AF219992 Genomic DNA. Translation: AAG44243.2. CT010362 mRNA. Translation: CAJ18569.1. BC024526 mRNA. Translation: AAH24526.1.
IPI	IPI00135626. IPI00230737. IPI00230738.
RefSeq	NP_033830.2. NM_009700.2.
UniGene	Mm.250786.
3D structure databases
ProteinModelPortal	P55088.
SMR	P55088. Positions 31-295.
ModBase	Search...
Protein-protein interaction databases
IntAct	P55088. 1 interaction.
MINT	MINT-4088102.
PTM databases
PhosphoSite	P55088.
Proteomic databases
PaxDb	P55088.
PRIDE	P55088.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000079081; ENSMUSP00000078088; ENSMUSG00000024411.
GeneID	11829.
KEGG	mmu:11829.
UCSC	uc008edq.2. mouse. uc008eds.2. mouse.
Organism-specific databases
CTD	361.
MGI	MGI:107387. Aqp4.
Phylogenomic databases
eggNOG	COG0580.
GeneTree	ENSGT00550000074347.
HOVERGEN	HBG000312.
KO	K09866.
OrthoDB	EOG46Q6T5.
Gene expression databases
ArrayExpress	P55088.
Bgee	P55088.
CleanEx	MM_AQP4.
Genevestigator	P55088.
GermOnline	ENSMUSG00000024411. Mus musculus.
Family and domain databases
Gene3D	1.20.1080.10. 1 hit.
InterPro	IPR023271. Aquaporin-like. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
PANTHER	PTHR19139. PTHR19139. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL5965.
ChiTaRS	AQP4. mouse.
NextBio	279739.
SOURCE	Search...

Entry information

Entry name

AQP4_MOUSE

Accession

Primary (citable) accession number: P55088
Secondary accession number(s): P97818

Q9EQI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 10, 2004
Last modified:	May 29, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents