ID   MFAP4_HUMAN             Reviewed;         255 AA.
AC   P55083; A8KAJ1; Q6P680;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   13-OCT-2009, entry version 74.
DE   RecName: Full=Microfibril-associated glycoprotein 4;
DE   Flags: Precursor;
GN   Name=MFAP4;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Placenta;
RX   MEDLINE=95359962; PubMed=7633408; DOI=10.1093/hmg/4.4.589;
RA   Zhao Z., Lee C.-C., Jiralerspong S., Juyal R.C., Lu F., Baldini A.,
RA   Greenberg F., Caskey C.T., Patel P.I.;
RT   "The gene for a human microfibril-associated glycoprotein is commonly
RT   deleted in Smith-Magenis syndrome patients.";
RL   Hum. Mol. Genet. 4:589-597(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Uterus;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=PNS;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   PROTEIN SEQUENCE OF 22-36.
RX   PubMed=15340161; DOI=10.1110/ps.04682504;
RA   Zhang Z., Henzel W.J.;
RT   "Signal peptide prediction based on analysis of experimentally
RT   verified cleavage sites.";
RL   Protein Sci. 13:2819-2824(2004).
RN   [6]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-137, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Plasma;
RX   PubMed=16335952; DOI=10.1021/pr0502065;
RA   Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E.,
RA   Moore R.J., Smith R.D.;
RT   "Human plasma N-glycoproteome analysis by immunoaffinity subtraction,
RT   hydrazide chemistry, and mass spectrometry.";
RL   J. Proteome Res. 4:2070-2080(2005).
RN   [7]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-87 AND ASN-137, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Liver;
RX   PubMed=19159218; DOI=10.1021/pr8008012;
RA   Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT   "Glycoproteomics analysis of human liver tissue by combination of
RT   multiple enzyme digestion and hydrazide chemistry.";
RL   J. Proteome Res. 8:651-661(2009).
CC   -!- FUNCTION: Could be involved in calcium-dependent cell adhesion or
CC       intercellular interactions.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC       matrix.
CC   -!- DISEASE: MFAP4 is deleted in the Smith-Magenis syndrome (SMS)
CC       [MIM:182290].
CC   -!- SIMILARITY: Contains 1 fibrinogen C-terminal domain.
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DR   EMBL; L38486; AAB00968.1; ALT_INIT; mRNA.
DR   EMBL; AK293056; BAF85745.1; -; mRNA.
DR   EMBL; CH471212; EAW50889.1; -; Genomic_DNA.
DR   EMBL; BC062415; AAH62415.1; -; mRNA.
DR   IPI; IPI00022792; -.
DR   RefSeq; NP_002395.1; -.
DR   UniGene; Hs.296049; -.
DR   HSSP; P02671; 1FZD.
DR   STRING; P55083; -.
DR   PRIDE; P55083; -.
DR   Ensembl; ENST00000299610; ENSP00000299610; ENSG00000166482; Homo sapiens.
DR   Ensembl; ENST00000395592; ENSP00000378957; ENSG00000166482; Homo sapiens.
DR   GeneID; 4239; -.
DR   KEGG; hsa:4239; -.
DR   UCSC; uc002gvt.1; human.
DR   CTD; 4239; -.
DR   GeneCards; GC17M019227; -.
DR   H-InvDB; HIX0039181; -.
DR   HGNC; HGNC:7035; MFAP4.
DR   MIM; 182290; phenotype.
DR   MIM; 600596; gene.
DR   Orphanet; 819; Smith-Magenis syndrome.
DR   PharmGKB; PA30771; -.
DR   HOGENOM; P55083; -.
DR   HOVERGEN; P55083; -.
DR   NextBio; 16715; -.
DR   ArrayExpress; P55083; -.
DR   Bgee; P55083; -.
DR   CleanEx; HS_MFAP4; -.
DR   Genevestigator; P55083; -.
DR   GermOnline; ENSG00000166482; Homo sapiens.
DR   GO; GO:0001527; C:microfibril; NAS:UniProtKB.
DR   GO; GO:0005509; F:calcium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0005102; F:receptor binding; IEA:InterPro.
DR   GO; GO:0007155; P:cell adhesion; NAS:UniProtKB.
DR   GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR   InterPro; IPR002181; Fibrinogen_a/b/g_C.
DR   InterPro; IPR014716; Fibrinogen_a/b/g_C_1.
DR   Gene3D; G3DSA:3.90.215.10; Fibrinogen_a/b/g_C_1; 1.
DR   Pfam; PF00147; Fibrinogen_C; 1.
DR   SMART; SM00186; FBG; 1.
DR   PROSITE; PS00514; FIBRINOGEN_C_1; FALSE_NEG.
DR   PROSITE; PS51406; FIBRINOGEN_C_2; 1.
PE   1: Evidence at protein level;
KW   Calcium; Cell adhesion; Complete proteome; Direct protein sequencing;
KW   Extracellular matrix; Glycoprotein; Secreted; Signal.
FT   SIGNAL        1     21
FT   CHAIN        22    255       Microfibril-associated glycoprotein 4.
FT                                /FTId=PRO_0000009134.
FT   DOMAIN       32    255       Fibrinogen C-terminal.
FT   MOTIF        26     28       Cell attachment site (Potential).
FT   CARBOHYD     87     87       N-linked (GlcNAc...).
FT   CARBOHYD    137    137       N-linked (GlcNAc...).
FT   CONFLICT    173    173       A -> V (in Ref. 4; AAH62415).
SQ   SEQUENCE   255 AA;  28648 MW;  B8F0B47AC694435E CRC64;
     MKALLALPLL LLLSTPPCAP QVSGIRGDAL ERFCLQQPLD CDDIYAQGYQ SDGVYLIYPS
     GPSVPVPVFC DMTTEGGKWT VFQKRFNGSV SFFRGWNDYK LGFGRADGEY WLGLQNMHLL
     TLKQKYELRV DLEDFENNTA YAKYADFSIS PNAVSAEEDG YTLFVAGFED GGAGDSLSYH
     SGQKFSTFDR DQDLFVQNCA ALSSGAFWFR SCHFANLNGF YLGGSHLSYA NGINWAQWKG
     FYYSLKRTEM KIRRA
//