Reviewed, UniProtKB/Swiss-Prot P55083 (MFAP4_HUMAN)

Names and origin

Protein names

Recommended name:
    Microfibril-associated glycoprotein 4

Gene names

Name:

MFAP4

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Could be involved in calcium-dependent cell adhesion or intercellular interactions.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Involvement in disease	MFAP4 is deleted in the Smith-Magenis syndrome (SMS) [MIM:182290].
Sequence similarities	Contains 1 fibrinogen C-terminal domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Extracellular matrix Secreted
Domain	Signal
Ligand	Calcium
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell adhesion Non-traceable author statement. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	microfibril Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.5
	Chain	22 – 255	234	Microfibril-associated glycoprotein 4		PRO_0000009134
Regions
	Domain	32 – 255	224	Fibrinogen C-terminal
	Motif	26 – 28	3	Cell attachment site Potential
Amino acid modifications
	Glycosylation	87	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	137	1	N-linked (GlcNAc...) Ref.7 Ref.6
Experimental info
	Sequence conflict	173	1	A → V in AAH62415. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P55083-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: B8F0B47AC694435E	FASTA	255	28,648
	10 20 30 40 50 60 MKALLALPLL LLLSTPPCAP QVSGIRGDAL ERFCLQQPLD CDDIYAQGYQ SDGVYLIYPS 70 80 90 100 110 120 GPSVPVPVFC DMTTEGGKWT VFQKRFNGSV SFFRGWNDYK LGFGRADGEY WLGLQNMHLL 130 140 150 160 170 180 TLKQKYELRV DLEDFENNTA YAKYADFSIS PNAVSAEEDG YTLFVAGFED GGAGDSLSYH 190 200 210 220 230 240 SGQKFSTFDR DQDLFVQNCA ALSSGAFWFR SCHFANLNGF YLGGSHLSYA NGINWAQWKG 250 FYYSLKRTEM KIRRA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene for a human microfibril-associated glycoprotein is commonly deleted in Smith-Magenis syndrome patients." Zhao Z., Lee C.-C., Jiralerspong S., Juyal R.C., Lu F., Baldini A., Greenberg F., Caskey C.T., Patel P.I. Hum. Mol. Genet. 4:589-597(1995) [PubMed: 7633408] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: PNS.
[5]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-36.
[6]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-137, MASS SPECTROMETRY. Tissue: Plasma.
[7]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-87 AND ASN-137, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L38486 mRNA. Translation: AAB00968.1. Different initiation. AK293056 mRNA. Translation: BAF85745.1. CH471212 Genomic DNA. Translation: EAW50889.1. BC062415 mRNA. Translation: AAH62415.1.
IPI	IPI00022792.
RefSeq	NP_002395.1.
UniGene	Hs.296049
3D structure databases
HSSP	HSSP built from PDB template 1FZD based on UniProtKB P02671.
ModBase	Search...
Protein-protein interaction databases
STRING	P55083.
Proteomic databases
PRIDE	P55083.
Genome annotation databases
Ensembl	ENST00000299610; ENSP00000299610; ENSG00000166482; Homo sapiens. [Genome view] ENST00000395592; ENSP00000378957; ENSG00000166482; Homo sapiens. [Genome view]
GeneID	4239.
KEGG	hsa:4239.
UCSC	uc002gvt.1. human.
Organism-specific databases
CTD	4239.
GeneCards	GC17M019227.
H-InvDB	HIX0039181.
HGNC	HGNC:7035. MFAP4.
MIM	182290. phenotype. 600596. gene.
Orphanet	819. Smith-Magenis syndrome.
PharmGKB	PA30771.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P55083.
HOVERGEN	P55083.
Gene expression databases
ArrayExpress	P55083.
Bgee	P55083.
CleanEx	HS_MFAP4.
Genevestigator	P55083.
GermOnline	ENSG00000166482. Homo sapiens.
Family and domain databases
InterPro	IPR002181. Fibrinogen_a/b/g_C. IPR014716. Fibrinogen_a/b/g_C_1. [Graphical view]
Gene3D	G3DSA:3.90.215.10. Fibrinogen_a/b/g_C_1. 1 hit.
Pfam	PF00147. Fibrinogen_C. 1 hit. [Graphical view]
SMART	SM00186. FBG. 1 hit. [Graphical view]
PROSITE	PS00514. FIBRINOGEN_C_1. False negative. PS51406. FIBRINOGEN_C_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	16715.
SOURCE	Search...

Entry information

Entry name

MFAP4_HUMAN

Accession

Primary (citable) accession number: P55083
Secondary accession number(s): A8KAJ1, Q6P680

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	October 13, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.