Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P55081

- MFAP1_HUMAN

UniProt

P55081 - MFAP1_HUMAN

Protein

Microfibrillar-associated protein 1

Gene

MFAP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (22 Jul 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the elastin-associated microfibrils.

    GO - Molecular functioni

    1. poly(A) RNA binding Source: UniProtKB
    2. protein binding Source: IntAct

    GO - Biological processi

    1. extracellular matrix organization Source: Reactome

    Enzyme and pathway databases

    ReactomeiREACT_150331. Molecules associated with elastic fibres.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microfibrillar-associated protein 1
    Gene namesi
    Name:MFAP1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:7032. MFAP1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome
    2. microfibril Source: UniProtKB

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30768.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 439438Microfibrillar-associated protein 1PRO_0000096458Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 Publications
    Modified residuei52 – 521Phosphoserine4 Publications
    Modified residuei53 – 531Phosphoserine4 Publications
    Modified residuei116 – 1161Phosphoserine7 Publications
    Modified residuei118 – 1181Phosphoserine7 Publications
    Modified residuei132 – 1321Phosphoserine4 Publications
    Modified residuei133 – 1331Phosphoserine4 Publications
    Modified residuei267 – 2671Phosphothreonine8 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP55081.
    PaxDbiP55081.
    PRIDEiP55081.

    PTM databases

    PhosphoSiteiP55081.

    Expressioni

    Gene expression databases

    BgeeiP55081.
    CleanExiHS_MFAP1.
    GenevestigatoriP55081.

    Organism-specific databases

    HPAiHPA042370.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    IKQ131232EBI-1048159,EBI-713456
    PRPF3O433952EBI-1048159,EBI-744322

    Protein-protein interaction databases

    BioGridi110394. 24 interactions.
    IntActiP55081. 12 interactions.
    MINTiMINT-1682969.
    STRINGi9606.ENSP00000267812.

    Structurei

    3D structure databases

    ProteinModelPortaliP55081.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi134 – 1396Poly-Glu

    Sequence similaritiesi

    Belongs to the MFAP1 family.Curated

    Phylogenomic databases

    eggNOGiNOG122136.
    HOGENOMiHOG000231845.
    HOVERGENiHBG052462.
    InParanoidiP55081.
    KOiK13110.
    OMAiHRHIHEP.
    OrthoDBiEOG7ZPNKJ.
    PhylomeDBiP55081.
    TreeFamiTF314398.

    Family and domain databases

    InterProiIPR009730. MFAP1_C.
    [Graphical view]
    PANTHERiPTHR15327. PTHR15327. 1 hit.
    PfamiPF06991. Prp19_bind. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P55081-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVPSALMKQ PPIQSTAGAV PVRNEKGEIS MEKVKVKRYV SGKRPDYAPM    50
    ESSDEEDEEF QFIKKAKEQE AEPEEQEEDS SSDPRLRRLQ NRISEDVEER 100
    LARHRKIVEP EVVGESDSEV EGDAWRMERE DSSEEEEEEI DDEEIERRRG 150
    MMRQRAQERK NEEMEVMEVE DEGRSGEESE SESEYEEYTD SEDEMEPRLK 200
    PVFIRKKDRV TVQEREAEAL KQKELEQEAK RMAEERRKYT LKIVEEETKK 250
    ELEENKRSLA ALDALNTDDE NDEEEYEAWK VRELKRIKRD REDREALEKE 300
    KAEIERMRNL TEEERRAELR ANGKVITNKA VKGKYKFLQK YYHRGAFFMD 350
    EDEEVYKRDF SAPTLEDHFN KTILPKVMQV KNFGRSGRTK YTHLVDQDTT 400
    SFDSAWGQES AQNTKFFKQK AAGVRDVFER PSAKKRKTT 439
    Length:439
    Mass (Da):51,958
    Last modified:July 22, 2008 - v2
    Checksum:i0EF6F4F091C05A88
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti238 – 2381K → Q in AAA92786. (PubMed:7835894)Curated
    Sequence conflicti242 – 2421K → Q in AAA92786. (PubMed:7835894)Curated
    Sequence conflicti245 – 2451E → G in AAA92786. (PubMed:7835894)Curated
    Sequence conflicti249 – 2491K → P in AAA92786. (PubMed:7835894)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04209 mRNA. Translation: AAA92786.1.
    AK312867 mRNA. Translation: BAG35719.1.
    AC018512 Genomic DNA. No translation available.
    CH471082 Genomic DNA. Translation: EAW77247.1.
    BC023557 mRNA. Translation: AAH23557.1.
    BC050742 mRNA. Translation: AAH50742.1.
    CCDSiCCDS10105.1.
    PIRiA55565.
    RefSeqiNP_005917.2. NM_005926.2.
    UniGeneiHs.61418.

    Genome annotation databases

    EnsembliENST00000267812; ENSP00000267812; ENSG00000140259.
    GeneIDi4236.
    KEGGihsa:4236.
    UCSCiuc001zth.1. human.

    Polymorphism databases

    DMDMi205831094.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04209 mRNA. Translation: AAA92786.1 .
    AK312867 mRNA. Translation: BAG35719.1 .
    AC018512 Genomic DNA. No translation available.
    CH471082 Genomic DNA. Translation: EAW77247.1 .
    BC023557 mRNA. Translation: AAH23557.1 .
    BC050742 mRNA. Translation: AAH50742.1 .
    CCDSi CCDS10105.1.
    PIRi A55565.
    RefSeqi NP_005917.2. NM_005926.2.
    UniGenei Hs.61418.

    3D structure databases

    ProteinModelPortali P55081.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110394. 24 interactions.
    IntActi P55081. 12 interactions.
    MINTi MINT-1682969.
    STRINGi 9606.ENSP00000267812.

    PTM databases

    PhosphoSitei P55081.

    Polymorphism databases

    DMDMi 205831094.

    Proteomic databases

    MaxQBi P55081.
    PaxDbi P55081.
    PRIDEi P55081.

    Protocols and materials databases

    DNASUi 4236.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000267812 ; ENSP00000267812 ; ENSG00000140259 .
    GeneIDi 4236.
    KEGGi hsa:4236.
    UCSCi uc001zth.1. human.

    Organism-specific databases

    CTDi 4236.
    GeneCardsi GC15M044096.
    H-InvDB HIX0026808.
    HGNCi HGNC:7032. MFAP1.
    HPAi HPA042370.
    MIMi 600215. gene.
    neXtProti NX_P55081.
    PharmGKBi PA30768.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG122136.
    HOGENOMi HOG000231845.
    HOVERGENi HBG052462.
    InParanoidi P55081.
    KOi K13110.
    OMAi HRHIHEP.
    OrthoDBi EOG7ZPNKJ.
    PhylomeDBi P55081.
    TreeFami TF314398.

    Enzyme and pathway databases

    Reactomei REACT_150331. Molecules associated with elastic fibres.

    Miscellaneous databases

    ChiTaRSi MFAP1. human.
    GeneWikii MFAP1.
    GenomeRNAii 4236.
    NextBioi 16701.
    PROi P55081.
    SOURCEi Search...

    Gene expression databases

    Bgeei P55081.
    CleanExi HS_MFAP1.
    Genevestigatori P55081.

    Family and domain databases

    InterProi IPR009730. MFAP1_C.
    [Graphical view ]
    PANTHERi PTHR15327. PTHR15327. 1 hit.
    Pfami PF06991. Prp19_bind. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the human gene encoding the associated microfibrillar protein (MFAP1) and localization to chromosome 15q15-q21."
      Yeh H., Chow M., Abrams W.R., Fan J., Foster J., Mitchell H., Muenke M., Rosenbloom J.
      Genomics 23:443-449(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Skin.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Trachea.
    3. "Analysis of the DNA sequence and duplication history of human chromosome 15."
      Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
      , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
      Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Muscle and PNS.
    6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116; SER-118; SER-132; SER-133 AND THR-267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line."
      Giorgianni F., Zhao Y., Desiderio D.M., Beranova-Giorgianni S.
      Electrophoresis 28:2027-2034(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116; SER-118 AND THR-267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Prostate cancer.
    8. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
      Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
      J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116; SER-118 AND THR-267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; SER-53 AND THR-267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
      Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.
      Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116; SER-118 AND THR-267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    12. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; SER-53; SER-116; SER-118; SER-132; SER-133 AND THR-267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; SER-53; SER-116; SER-118; SER-132; SER-133 AND THR-267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    15. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; SER-53; SER-116; SER-118; SER-132; SER-133 AND THR-267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    17. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    18. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMFAP1_HUMAN
    AccessioniPrimary (citable) accession number: P55081
    Secondary accession number(s): Q86TG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 22, 2008
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3