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Protein

Brevican core protein

Gene

Bcan

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans. Isoform 2 may function as a chondroitin sulfate-bearing cell surface receptor.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Hyaluronic acid, Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Brevican core protein
Alternative name(s):
Brain-enriched hyaluronan-binding protein
Short name:
BEHAB
Gene namesi
Name:Bcan
Synonyms:Behab
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2194. Bcan.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: RGD
  • dendrite Source: RGD
  • extracellular space Source: RGD
  • proteinaceous extracellular matrix Source: GO_Central
  • synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000001751323 – 883Brevican core proteinAdd BLAST861

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi56 ↔ 136By similarity
Glycosylationi129N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi178 ↔ 249By similarity
Disulfide bondi202 ↔ 223By similarity
Disulfide bondi276 ↔ 351By similarity
Disulfide bondi300 ↔ 321By similarity
Glycosylationi336N-linked (GlcNAc...)Sequence analysis1
Modified residuei413PhosphoserineCombined sources1
Disulfide bondi626 ↔ 637By similarity
Disulfide bondi631 ↔ 646By similarity
Disulfide bondi648 ↔ 657By similarity
Disulfide bondi692 ↔ 784By similarity
Disulfide bondi760 ↔ 776By similarity
Disulfide bondi791 ↔ 834By similarity
Disulfide bondi820 ↔ 847By similarity

Post-translational modificationi

Contains mostly chondroitin sulfate.
The GPI-anchor may be located on Ser-622 of isoform 2.Curated

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein, Proteoglycan

Proteomic databases

PaxDbiP55068.
PRIDEiP55068.

PTM databases

iPTMnetiP55068.
PhosphoSitePlusiP55068.

Miscellaneous databases

PMAP-CutDBP55068.

Expressioni

Tissue specificityi

Brain.

Developmental stagei

Isoform 1 increases from day P4 to P64. Isoform 2 increases after day P8.

Interactioni

Subunit structurei

Interacts with TNR.1 Publication

Protein-protein interaction databases

BioGridi247431. 1 interactor.
STRINGi10116.ENSRNOP00000025496.

Structurei

3D structure databases

ProteinModelPortaliP55068.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 154Ig-like V-typeAdd BLAST120
Domaini156 – 251Link 1PROSITE-ProRule annotationAdd BLAST96
Domaini256 – 353Link 2PROSITE-ProRule annotationAdd BLAST98
Domaini622 – 658EGF-likePROSITE-ProRule annotationAdd BLAST37
Domaini658 – 786C-type lectinPROSITE-ProRule annotationAdd BLAST129
Domaini789 – 849SushiPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 2 Link domains.PROSITE-ProRule annotation
Contains 1 Sushi (CCP/SCR) domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Immunoglobulin domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiENOG410IG1W. Eukaryota.
ENOG410XW3U. LUCA.
HOGENOMiHOG000115520.
HOVERGENiHBG008175.
InParanoidiP55068.
KOiK06795.
PhylomeDBiP55068.

Family and domain databases

CDDicd00033. CCP. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.10.100.10. 3 hits.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR016187. CTDL_fold.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR000538. Link_dom.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 1 hit.
PF07686. V-set. 1 hit.
PF00193. Xlink. 2 hits.
[Graphical view]
PRINTSiPR01265. LINKMODULE.
SMARTiSM00032. CCP. 1 hit.
SM00034. CLECT. 1 hit.
SM00181. EGF. 1 hit.
SM00409. IG. 1 hit.
SM00406. IGv. 1 hit.
SM00445. LINK. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF56436. SSF56436. 3 hits.
SSF57535. SSF57535. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
PS01241. LINK_1. 2 hits.
PS50963. LINK_2. 2 hits.
PS50923. SUSHI. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P55068-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIPLLLSLLA ALVLTQAPAA LADDLKEDSS EDRAFRVRIG AAQLRGVLGG
60 70 80 90 100
ALAIPCHVHH LRPPPSRRAA PGFPRVKWTF LSGDREVEVL VARGLRVKVN
110 120 130 140 150
EAYRFRVALP AYPASLTDVS LVLSELRPND SGVYRCEVQH GIDDSSDAVE
160 170 180 190 200
VKVKGVVFLY REGSARYAFS FAGAQEACAR IGARIATPEQ LYAAYLGGYE
210 220 230 240 250
QCDAGWLSDQ TVRYPIQNPR EACYGDMDGY PGVRNYGVVG PDDLYDVYCY
260 270 280 290 300
AEDLNGELFL GAPPGKLTWE EARDYCLERG AQIASTGQLY AAWNGGLDRC
310 320 330 340 350
SPGWLADGSV RYPIITPSQR CGGGLPGVKT LFLFPNQTGF PSKQNRFNVY
360 370 380 390 400
CFRDSAHPSA FSEASSPASD GLEAIVTVTE KLEELQLPQE AVESESRGAI
410 420 430 440 450
YSIPITEDGG GGSSTPEDPA EAPRTPLESE TQSVAPPTGS SEEEGEALEE
460 470 480 490 500
EERFKDTETP KEEKEQENLW VWPTELSSPL PTGLETEHSL SQVSPPAQAV
510 520 530 540 550
LQVGASPSPR PPRVHGPTVE TLQPPGEGSL TSTPDGAREV GGETGSPELS
560 570 580 590 600
GVPREREEAG SSSLEDGPSL LPETWAPVGT REVETPSEEK SGRTVLTGTS
610 620 630 640 650
VQAQPVLPTD SASRGGVAVA PSSGDCIPSP CHNGGTCLEE KEGFRCLCVP
660 670 680 690 700
GYGGDLCDVG LHFCSPGWEP FQGACYKHFS TRRSWEEAES QCRALGAHLT
710 720 730 740 750
SICTPEEQDF VNDRYREYQW IGLNDRTIEG DFLWSDGPPL LYENWNPGQP
760 770 780 790 800
DSYFLSGENC VVMVWHDQGQ WSDVPCNYHL SYTCKMGLVS CGPPPQLPLA
810 820 830 840 850
QIFGRPRLRY AVDTVLRYRC RDGLAQRNLP LIRCQENGLW EAPQISCVPR
860 870 880
RPARALRSMT APEGPRGQLP RQRKALLTPP SSL
Length:883
Mass (Da):96,057
Last modified:November 1, 1997 - v2
Checksum:iAC7ACC40CB53ED37
GO
Isoform 2 (identifier: P55068-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     625-645: DCIPSPCHNGGTCLEEKEGFR → NSAEGSMPAFLLFLLLQLWDT
     646-883: Missing.

Note: GPI-anchor amidated serine on Ser-622.Curated
Show »
Length:645
Mass (Da):69,085
Checksum:i1BC9B7FF08970B89
GO

Sequence cautioni

The sequence CAA82215 differs from that shown. Reason: Frameshift at position 364.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51 – 52AL → WV in CAA82215 (PubMed:7512973).Curated2
Sequence conflicti503V → L in AAA87847 (PubMed:7488217).Curated1
Sequence conflicti518 – 519TV → PA in AAA87847 (PubMed:7488217).Curated2
Sequence conflicti526G → R in AAA87847 (PubMed:7488217).Curated1
Sequence conflicti541G → A in AAA87847 (PubMed:7488217).Curated1
Sequence conflicti556R → S in AAA87847 (PubMed:7488217).Curated1
Sequence conflicti573E → A in AAA87847 (PubMed:7488217).Curated1
Sequence conflicti583V → L in AAA87847 (PubMed:7488217).Curated1
Sequence conflicti649V → L in AAA87847 (PubMed:7488217).Curated1
Sequence conflicti670P → A in AAA87847 (PubMed:7488217).Curated1
Sequence conflicti738P → A in AAA87847 (PubMed:7488217).Curated1
Sequence conflicti809R → A in AAA87847 (PubMed:7488217).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003076625 – 645DCIPS…KEGFR → NSAEGSMPAFLLFLLLQLWD T in isoform 2. CuratedAdd BLAST21
Alternative sequenceiVSP_003077646 – 883Missing in isoform 2. CuratedAdd BLAST238

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79881 mRNA. Translation: CAA56255.1.
X86406 mRNA. Translation: CAA60160.1.
U37142 mRNA. Translation: AAA87847.1.
Z28366 mRNA. Translation: CAA82215.1. Frameshift.
PIRiA53908.
S49126.
RefSeqiNP_001028837.1. NM_001033665.1.
NP_037048.2. NM_012916.2.
UniGeneiRn.10315.
Rn.168035.

Genome annotation databases

GeneIDi25393.
KEGGirno:25393.
UCSCiRGD:2194. rat. [P55068-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79881 mRNA. Translation: CAA56255.1.
X86406 mRNA. Translation: CAA60160.1.
U37142 mRNA. Translation: AAA87847.1.
Z28366 mRNA. Translation: CAA82215.1. Frameshift.
PIRiA53908.
S49126.
RefSeqiNP_001028837.1. NM_001033665.1.
NP_037048.2. NM_012916.2.
UniGeneiRn.10315.
Rn.168035.

3D structure databases

ProteinModelPortaliP55068.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247431. 1 interactor.
STRINGi10116.ENSRNOP00000025496.

PTM databases

iPTMnetiP55068.
PhosphoSitePlusiP55068.

Proteomic databases

PaxDbiP55068.
PRIDEiP55068.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25393.
KEGGirno:25393.
UCSCiRGD:2194. rat. [P55068-1]

Organism-specific databases

CTDi63827.
RGDi2194. Bcan.

Phylogenomic databases

eggNOGiENOG410IG1W. Eukaryota.
ENOG410XW3U. LUCA.
HOGENOMiHOG000115520.
HOVERGENiHBG008175.
InParanoidiP55068.
KOiK06795.
PhylomeDBiP55068.

Miscellaneous databases

PMAP-CutDBP55068.
PROiP55068.

Family and domain databases

CDDicd00033. CCP. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.10.100.10. 3 hits.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR016187. CTDL_fold.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR000538. Link_dom.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 1 hit.
PF07686. V-set. 1 hit.
PF00193. Xlink. 2 hits.
[Graphical view]
PRINTSiPR01265. LINKMODULE.
SMARTiSM00032. CCP. 1 hit.
SM00034. CLECT. 1 hit.
SM00181. EGF. 1 hit.
SM00409. IG. 1 hit.
SM00406. IGv. 1 hit.
SM00445. LINK. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF56436. SSF56436. 3 hits.
SSF57535. SSF57535. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
PS01241. LINK_1. 2 hits.
PS50963. LINK_2. 2 hits.
PS50923. SUSHI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGCB_RAT
AccessioniPrimary (citable) accession number: P55068
Secondary accession number(s): Q62860, Q63040, Q63513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.