Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P55066

- NCAN_MOUSE

UniProt

P55066 - NCAN_MOUSE

Protein

Neurocan core protein

Gene

Ncan

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. carbohydrate binding Source: InterPro
    3. hyaluronic acid binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. regulation of synapse structural plasticity Source: MGI

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Calcium, Hyaluronic acid, Lectin

    Enzyme and pathway databases

    ReactomeiREACT_196489. A tetrasaccharide linker sequence is required for GAG synthesis.
    REACT_196514. Chondroitin sulfate biosynthesis.
    REACT_196540. Dermatan sulfate biosynthesis.
    REACT_196606. ECM proteoglycans.
    REACT_198981. CS/DS degradation.
    REACT_219680. L1CAM interactions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neurocan core protein
    Alternative name(s):
    Chondroitin sulfate proteoglycan 3
    Gene namesi
    Name:Ncan
    Synonyms:Cspg3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:104694. Ncan.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 12681246Neurocan core proteinPRO_0000017517Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi58 ↔ 139By similarity
    Glycosylationi121 – 1211N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi181 ↔ 252By similarity
    Disulfide bondi205 ↔ 226By similarity
    Disulfide bondi279 ↔ 354By similarity
    Disulfide bondi303 ↔ 324By similarity
    Glycosylationi339 – 3391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi742 – 7421N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi964 ↔ 975By similarity
    Disulfide bondi969 ↔ 984By similarity
    Glycosylationi978 – 9781N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi986 ↔ 995By similarity
    Disulfide bondi1002 ↔ 1013By similarity
    Disulfide bondi1007 ↔ 1022By similarity
    Disulfide bondi1024 ↔ 1033By similarity
    Disulfide bondi1040 ↔ 1051By similarity
    Disulfide bondi1068 ↔ 1160By similarity
    Disulfide bondi1136 ↔ 1152By similarity
    Disulfide bondi1167 ↔ 1210By similarity
    Glycosylationi1175 – 11751N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi1196 ↔ 1223By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Proteoglycan

    Proteomic databases

    PaxDbiP55066.
    PRIDEiP55066.

    PTM databases

    PhosphoSiteiP55066.

    Expressioni

    Tissue specificityi

    Brain.

    Gene expression databases

    BgeeiP55066.
    CleanExiMM_NCAN.
    GenevestigatoriP55066.

    Interactioni

    Protein-protein interaction databases

    IntActiP55066. 2 interactions.
    MINTiMINT-4092285.

    Structurei

    3D structure databases

    ProteinModelPortaliP55066.
    SMRiP55066. Positions 40-146, 160-253, 269-358, 949-1163.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 157121Ig-like V-typeAdd
    BLAST
    Domaini159 – 25496Link 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini258 – 35699Link 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini960 – 99637EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini998 – 103437EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini1036 – 1165130C-type lectinPROSITE-ProRule annotationAdd
    BLAST
    Domaini1165 – 122561SushiPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C-type lectin domain.PROSITE-ProRule annotation
    Contains 2 EGF-like domains.PROSITE-ProRule annotation
    Contains 2 Link domains.PROSITE-ProRule annotation
    Contains 1 Sushi (CCP/SCR) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Immunoglobulin domain, Repeat, Signal, Sushi

    Phylogenomic databases

    eggNOGiNOG147231.
    GeneTreeiENSGT00750000117329.
    HOGENOMiHOG000170487.
    HOVERGENiHBG078994.
    InParanoidiP55066.
    KOiK06794.
    OrthoDBiEOG7FFMQR.
    PhylomeDBiP55066.
    TreeFamiTF332134.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    3.10.100.10. 3 hits.
    InterProiIPR001304. C-type_lectin.
    IPR016186. C-type_lectin-like.
    IPR018378. C-type_lectin_CS.
    IPR016187. C-type_lectin_fold.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    IPR000538. Link.
    IPR000436. Sushi_SCR_CCP.
    [Graphical view]
    PfamiPF00059. Lectin_C. 1 hit.
    PF00084. Sushi. 1 hit.
    PF07686. V-set. 1 hit.
    PF00193. Xlink. 2 hits.
    [Graphical view]
    PRINTSiPR01265. LINKMODULE.
    SMARTiSM00032. CCP. 1 hit.
    SM00034. CLECT. 1 hit.
    SM00181. EGF. 1 hit.
    SM00179. EGF_CA. 1 hit.
    SM00409. IG. 1 hit.
    SM00445. LINK. 2 hits.
    [Graphical view]
    SUPFAMiSSF56436. SSF56436. 3 hits.
    SSF57535. SSF57535. 1 hit.
    PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
    PS00615. C_TYPE_LECTIN_1. 1 hit.
    PS50041. C_TYPE_LECTIN_2. 1 hit.
    PS00022. EGF_1. 3 hits.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS01187. EGF_CA. 1 hit.
    PS50835. IG_LIKE. 1 hit.
    PS01241. LINK_1. 2 hits.
    PS50963. LINK_2. 2 hits.
    PS50923. SUSHI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P55066-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGAGSVWASG LLLLWLLLLV AGDQDTQDTT ATEKGLRMLK SGSGPVRAAL     50
    AELVALPCFF TLQPRLSSLR DIPRIKWTKV QTASGQRQDL PILVAKDNVV 100
    RVAKGWQGRV SLPAYPRHRA NATLLLGPLR ASDSGLYRCQ VVKGIEDEQD 150
    LVTLEVTGVV FHYRAARDRY ALTFAEAQEA CRLSSATIAA PRHLQAAFED 200
    GFDNCDAGWL SDRTVRYPIT QSRPGCYGDR SSLPGVRSYG RRDPQELYDV 250
    YCFARELGGE VFYVGPARRL TLAGARAQCQ RQGAALASVG QLHLAWHEGL 300
    DQCDPGWLAD GSVRYPIQTP RRRCGGPAPG VRTVYRFANR TGFPAPGARF 350
    DAYCFRAHHH TAQHGDSEIP SSGDEGEIVS AEGPPGRELK PSLGEQEVIA 400
    PDFQEPLMSS GEGEPPDLTW TQAPEETLGS TPGGPTLASW PSSEKWLFTG 450
    APSSMGVSSP SDMGVDMEAT TPLGTQVAPT PTMRRGRFKG LNGRHFQQQG 500
    PEDQLPEVAE PSAQPPTLGA TANHMRPSAA TEASESDQSH SPWAILTNEV 550
    DEPGAGSLGS RSLPESLMWS PSLISPSVPS TESTPSPKPG AAEAPSVKSA 600
    IPHLPRLPSE PPAPSPGPSE ALSAVSLQAS SADGSPDFPI VAMLRAPKLW 650
    LLPRSTLVPN MTPVPLSPAS PLPSWVPEEQ AVRPVSLGAE DLETPFQTTI 700
    AAPVEASHRS PDADSIEIEG TSSMRATKHP ISGPWASLDS SNVTMNPVPS 750
    DAGILGTESG VLDLPGSPTS GGQATVEKVL ATWLPLPGQG LDPGSQSTPM 800
    EAHGVAVSME PTVALEGGAT EGPMEATREV VPSTADATWE SESRSAISST 850
    HIAVTMARAQ GMPTLTSTSS EGHPEPKGQM VAQESLEPLN TLPSHPWSSL 900
    VVPMDEVASV SSGEPTGLWD IPSTLIPVSL GLDESVLNVV AESPSVEGFW 950
    EEVASGQEDP TDPCENNPCL HGGTCHTNGT VYGCSCDQGY AGENCEIDID 1000
    DCLCSPCENG GTCIDEVNGF ICLCLPSYGG SLCEKDTEGC DRGWHKFQGH 1050
    CYRYFAHRRA WEDAERDCRR RAGHLTSVHS PEEHKFINSF GHENSWIGLN 1100
    DRTVERDFQW TDNTGLQYEN WREKQPDNFF AGGEDCVVMV AHESGRWNDV 1150
    PCNYNLPYVC KKGTVLCGPP PAVENASLVG VRKIKYNVHA TVRYQCDEGF 1200
    SQHRVATIRC RNNGKWDRPQ IMCIKPRRSH RMRRHHHHPH RHHKPRKEHR 1250
    KHKRHPAEDW EKDEGDFC 1268
    Length:1,268
    Mass (Da):137,200
    Last modified:October 1, 1996 - v1
    Checksum:i3014E8E202A2FAEC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti582 – 5821E → D in AAH65118. (PubMed:15489334)Curated
    Sequence conflicti587 – 5871P → A in AAH65118. (PubMed:15489334)Curated
    Sequence conflicti936 – 9361V → D in AAH65118. (PubMed:15489334)Curated
    Sequence conflicti947 – 9471E → K in AAH65118. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84727 mRNA. Translation: CAA59216.1.
    BC065118 mRNA. Translation: AAH65118.1.
    AK082298 mRNA. Translation: BAC38458.1.
    CCDSiCCDS22358.1.
    PIRiS52781.
    RefSeqiNP_031815.2. NM_007789.3.
    UniGeneiMm.268079.

    Genome annotation databases

    EnsembliENSMUST00000002412; ENSMUSP00000002412; ENSMUSG00000002341.
    GeneIDi13004.
    KEGGimmu:13004.
    UCSCiuc009lys.2. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - Glycan Binding

    Neurocan

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84727 mRNA. Translation: CAA59216.1 .
    BC065118 mRNA. Translation: AAH65118.1 .
    AK082298 mRNA. Translation: BAC38458.1 .
    CCDSi CCDS22358.1.
    PIRi S52781.
    RefSeqi NP_031815.2. NM_007789.3.
    UniGenei Mm.268079.

    3D structure databases

    ProteinModelPortali P55066.
    SMRi P55066. Positions 40-146, 160-253, 269-358, 949-1163.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P55066. 2 interactions.
    MINTi MINT-4092285.

    PTM databases

    PhosphoSitei P55066.

    Proteomic databases

    PaxDbi P55066.
    PRIDEi P55066.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002412 ; ENSMUSP00000002412 ; ENSMUSG00000002341 .
    GeneIDi 13004.
    KEGGi mmu:13004.
    UCSCi uc009lys.2. mouse.

    Organism-specific databases

    CTDi 1463.
    MGIi MGI:104694. Ncan.

    Phylogenomic databases

    eggNOGi NOG147231.
    GeneTreei ENSGT00750000117329.
    HOGENOMi HOG000170487.
    HOVERGENi HBG078994.
    InParanoidi P55066.
    KOi K06794.
    OrthoDBi EOG7FFMQR.
    PhylomeDBi P55066.
    TreeFami TF332134.

    Enzyme and pathway databases

    Reactomei REACT_196489. A tetrasaccharide linker sequence is required for GAG synthesis.
    REACT_196514. Chondroitin sulfate biosynthesis.
    REACT_196540. Dermatan sulfate biosynthesis.
    REACT_196606. ECM proteoglycans.
    REACT_198981. CS/DS degradation.
    REACT_219680. L1CAM interactions.

    Miscellaneous databases

    NextBioi 282828.
    PROi P55066.
    SOURCEi Search...

    Gene expression databases

    Bgeei P55066.
    CleanExi MM_NCAN.
    Genevestigatori P55066.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    3.10.100.10. 3 hits.
    InterProi IPR001304. C-type_lectin.
    IPR016186. C-type_lectin-like.
    IPR018378. C-type_lectin_CS.
    IPR016187. C-type_lectin_fold.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    IPR000538. Link.
    IPR000436. Sushi_SCR_CCP.
    [Graphical view ]
    Pfami PF00059. Lectin_C. 1 hit.
    PF00084. Sushi. 1 hit.
    PF07686. V-set. 1 hit.
    PF00193. Xlink. 2 hits.
    [Graphical view ]
    PRINTSi PR01265. LINKMODULE.
    SMARTi SM00032. CCP. 1 hit.
    SM00034. CLECT. 1 hit.
    SM00181. EGF. 1 hit.
    SM00179. EGF_CA. 1 hit.
    SM00409. IG. 1 hit.
    SM00445. LINK. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56436. SSF56436. 3 hits.
    SSF57535. SSF57535. 1 hit.
    PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
    PS00615. C_TYPE_LECTIN_1. 1 hit.
    PS50041. C_TYPE_LECTIN_2. 1 hit.
    PS00022. EGF_1. 3 hits.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS01187. EGF_CA. 1 hit.
    PS50835. IG_LIKE. 1 hit.
    PS01241. LINK_1. 2 hits.
    PS50963. LINK_2. 2 hits.
    PS50923. SUSHI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and chromosomal localization of the mouse neurocan gene."
      Rauch U., Grimpe B., Kulbe G., Arnold-Ammer I., Beier D., Faessler R.
      Genomics 28:405-410(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1052-1268.
      Strain: C57BL/6J.
      Tissue: Cerebellum.

    Entry informationi

    Entry nameiNCAN_MOUSE
    AccessioniPrimary (citable) accession number: P55066
    Secondary accession number(s): Q6P1E3, Q8C4F8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3