P55064 (AQP5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aquaporin-5
Short name=AQP-5

Gene names

Name:

AQP5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions.
Subcellular location	Membrane; Multi-pass membrane protein.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbon dioxide transport Inferred from direct assay. Source: UniProtKB excretion Traceable author statement. Source: ProtInc odontogenesis Inferred from expression pattern. Source: UniProtKB pancreatic juice secretion Inferred from expression pattern. Source: UniProtKB
Cellular component	apical plasma membrane Inferred from direct assay. Source: UniProtKB integral to plasma membrane Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct water channel activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MDFI	Q99750	3	EBI-746103,EBI-724076

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 265

265

Aquaporin-5

PRO_0000063951

Regions

Topological domain

1 – 12

12

Cytoplasmic Potential

Transmembrane

13 – 33

21

Helical; Potential

Topological domain

34 – 36

3

Extracellular Potential

Transmembrane

37 – 57

21

Helical; Potential

Topological domain

58 – 87

30

Cytoplasmic Potential

Transmembrane

88 – 108

21

Helical; Potential

Topological domain

109 – 126

18

Extracellular Potential

Transmembrane

127 – 147

21

Helical; Potential

Topological domain

148 – 161

14

Cytoplasmic Potential

Transmembrane

162 – 182

21

Helical; Potential

Topological domain

183 – 205

23

Extracellular Potential

Transmembrane

206 – 226

21

Helical; Potential

Topological domain

227 – 265

39

Cytoplasmic Potential

Motif

69 – 71

3

NPA 1

Motif

185 – 187

3

NPA 2

Amino acid modifications

Glycosylation

124

1

N-linked (GlcNAc...) Potential

Glycosylation

125

1

N-linked (GlcNAc...) Potential

Secondary structure

1

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265

Helix Strand Turn

Details...

Helix

2 – 5

4

Helix

8 – 26

19

Beta strand

36 – 38

3

Helix

42 – 48

7

Helix

56 – 59

4

Helix

70 – 77

8

Helix

84 – 95

12

Turn

111 – 113

3

Helix

131 – 150

20

Helix

161 – 164

4

Turn

168 – 170

3

Helix

186 – 189

4

Helix

193 – 197

5

Helix

203 – 207

5

Helix

215 – 223

9

Turn

233 – 235

3

Helix

236 – 239

4

Sequences

	Sequence		Length	Mass (Da)
	P55064 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 053C10E6A17EAFDA	FASTA	265	28,292
	10 20 30 40 50 60 MKKEVCSVAF LKAVFAEFLA TLIFVFFGLG SALKWPSALP TILQIALAFG LAIGTLAQAL 70 80 90 100 110 120 GPVSGGHINP AITLALLVGN QISLLRAFFY VAAQLVGAIA GAGILYGVAP LNARGNLAVN 130 140 150 160 170 180 ALNNNTTQGQ AMVVELILTF QLALCIFAST DSRRTSPVGS PALSIGLSVT LGHLVGIYFT 190 200 210 220 230 240 GCSMNPARSF GPAVVMNRFS PAHWVFWVGP IVGAVLAAIL YFYLLFPNSL SLSERVAIIK 250 260 GTYEPDEDWE EQREERKKTM ELTTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human aquaporin-5 gene. Molecular characterization and chromosomal localization." Lee M.D., Bhakta K.Y., Raina S., Yonescu R., Griffin C.A., Copeland N.G., Gilbert D.J., Jenkins N.A., Preston G.M., Agre P. J. Biol. Chem. 271:8599-8604(1996) [PubMed: 8621489] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U46569 , U46566, U46567, U46568 Genomic DNA. Translation: AAC50474.1.
CR541989 mRNA. Translation: CAG46786.1.
CR542022 mRNA. Translation: CAG46819.1.
BC032946 mRNA. Translation: AAH32946.1.

IPI

IPI00022752.

RefSeq

NP_001642.1. NM_001651.2.

UniGene

Hs.298023.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3D9S	X-ray	2.00	A/B/C/D	2-265	[»]

ProteinModelPortal

P55064.

SMR

P55064. Positions 2-245.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46292N.

IntAct

P55064. 3 interactions.

MINT

MINT-1442891.

STRING

P55064.

Protein family/group databases

TCDB

1.A.8.8.9. major intrinsic protein (MIP) family.

PTM databases

PhosphoSite

P55064.

Polymorphism databases

DMDM

1703358.

Proteomic databases

PRIDE

P55064.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000293599; ENSP00000293599; ENSG00000161798.

GeneID

362.

KEGG

hsa:362.

UCSC

uc001rvo.2. human.

Organism-specific databases

CTD

362.

GeneCards

GC12P050411.

H-InvDB

HIX0010618.

HGNC

HGNC:638. AQP5.

MIM

600442. gene.

neXtProt

NX_P55064.

PharmGKB

PA24923.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG16715.

GeneTree

ENSGT00550000074347.

HOGENOM

HBG705794.

HOVERGEN

HBG000312.

InParanoid

P55064.

OMA

RALYYIV.

OrthoDB

EOG4FJ89M.

PhylomeDB

P55064.

Enzyme and pathway databases

Reactome

REACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpress

P55064.

Bgee

P55064.

CleanEx

HS_AQP5.

Genevestigator

P55064.

GermOnline

ENSG00000161798. Homo sapiens.

Family and domain databases

InterPro

IPR012269. Aquaporin.
IPR023271. Aquaporin-like.
IPR023276. Aquaporin_5.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]

Gene3D

G3DSA:1.20.1080.10. MIP. 1 hit.

KO

K09867.

PANTHER

PTHR19139. MIP. 1 hit.

Pfam

PF00230. MIP. 1 hit.
[Graphical view]

PRINTS

PR02017. AQUAPORIN5.
PR00783. MINTRINSICP.

SUPFAM

SSF81338. MIP. 1 hit.

TIGRFAMs

TIGR00861. MIP. 1 hit.

PROSITE

PS00221. MIP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

1515.

SOURCE

Search...

Entry information

Entry name

AQP5_HUMAN

Accession

Primary (citable) accession number: P55064
Secondary accession number(s): Q6FGW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.