SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P55059

- PDI_HUMIN

UniProt

P55059 - PDI_HUMIN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Protein disulfide-isomerase
Gene
N/A
Organism
Humicola insolens (Soft-rot fungus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Participates in the folding of proteins containing disulfide bonds, may be involved in glycosylation, prolyl hydroxylation and triglyceride transfer By similarity.

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei50 – 501Nucleophile By similarity
Sitei51 – 511Contributes to redox potential value By similarity
Sitei52 – 521Contributes to redox potential value By similarity
Active sitei53 – 531Nucleophile By similarity
Sitei113 – 1131Lowers pKa of C-terminal Cys of first active site By similarity
Active sitei385 – 3851Nucleophile By similarity
Sitei386 – 3861Contributes to redox potential value By similarity
Sitei387 – 3871Contributes to redox potential value By similarity
Active sitei388 – 3881Nucleophile By similarity
Sitei451 – 4511Lowers pKa of C-terminal Cys of second active site By similarity

GO - Molecular functioni

  1. protein disulfide isomerase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase (EC:5.3.4.1)
Short name:
PDI
OrganismiHumicola insolens (Soft-rot fungus)
Taxonomic identifieri34413 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesChaetomiaceaeHumicola

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed prediction
Add
BLAST
Chaini21 – 505485Protein disulfide-isomerase
PRO_0000034215Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 53Redox-active By similarity
Disulfide bondi385 ↔ 388Redox-active By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP55059.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi230 – 2323
Helixi235 – 2439
Beta strandi248 – 2525
Beta strandi256 – 2583
Helixi259 – 27113
Turni274 – 2763
Beta strandi277 – 2826
Turni284 – 2874
Helixi288 – 2903
Turni291 – 2955
Beta strandi298 – 30710
Turni309 – 3113
Beta strandi314 – 3163
Beta strandi319 – 3213
Helixi325 – 33612
Beta strandi356 – 3594
Turni362 – 3676
Beta strandi368 – 3703
Beta strandi372 – 3743
Beta strandi376 – 3816
Helixi388 – 40215
Turni405 – 4095
Beta strandi410 – 4178
Turni418 – 4203
Beta strandi428 – 4369
Beta strandi445 – 4484
Helixi453 – 46210
Beta strandi463 – 4664

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DJJNMR-A354-469[»]
2DJKNMR-A228-355[»]
2KP1NMR-A354-469[»]
2KP2NMR-A228-355[»]
ProteinModelPortaliP55059.
SMRiP55059. Positions 226-469.

Miscellaneous databases

EvolutionaryTraceiP55059.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 128108Thioredoxin 1
Add
BLAST
Domaini335 – 465131Thioredoxin 2
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi502 – 5054Prevents secretion from ER Reviewed prediction

Sequence similaritiesi

Contains 2 thioredoxin domains.

Keywords - Domaini

Redox-active center, Repeat, Signal

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55059-1 [UniParc]FASTAAdd to Basket

« Hide

MHKAQKFALG LLAAAAVATA SDVVQLKKDT FDDFIKTNDL VLAEFFAPWC    50
GHCKALAPEY EEAATTLKEK NIKLAKVDCT EETDLCQQHG VEGYPTLKVF 100
RGLDNVSPYK GQRKAAAITS YMIKQSLPAV SEVTKDNLEE FKKADKAVLV 150
AYVDASDKAS SEVFTQVAEK LRDNYPFGSS SDAALAEAEG VKAPAIVLYK 200
DFDEGKAVFS EKFEVEAIEK FAKTGATPLI GEIGPETYSD YMSAGIPLAY 250
IFAETAEERK ELSDKLKPIA EAQRGVINFG TIDAKAFGAH AGNLNLKTDK 300
FPAFAIQEVA KNQKFPFDQE KEITFEAIKA FVDDFVAGKI EPSIKSEPIP 350
EKQEGPVTVV VAKNYNEIVL DDTKDVLIEF YAPWCGHCKA LAPKYEELGA 400
LYAKSEFKDR VVIAKVDATA NDVPDEIQGF PTIKLYPAGA KGQPVTYSGS 450
RTVEDLIKFI AENGKYKAAI SEDAEETSSA TETTTETATK SEEAAKETAT 500
EHDEL 505
Length:505
Mass (Da):55,114
Last modified:October 1, 1996 - v1
Checksum:i27E7C0D1A61B5F95
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S74296 mRNA. Translation: AAC60578.1.
PIRiJC2291.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S74296 mRNA. Translation: AAC60578.1 .
PIRi JC2291.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2DJJ NMR - A 354-469 [» ]
2DJK NMR - A 228-355 [» ]
2KP1 NMR - A 354-469 [» ]
2KP2 NMR - A 228-355 [» ]
ProteinModelPortali P55059.
SMRi P55059. Positions 226-469.
ModBasei Search...

Proteomic databases

PRIDEi P55059.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P55059.

Family and domain databases

Gene3Di 3.40.30.10. 3 hits.
InterProi IPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF00085. Thioredoxin. 2 hits.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 4 hits.
TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of a fungal cDNA encoding protein disulfide isomerase."
    Kajino T., Sarai K., Imaeda T., Idekoba C., Asami O., Yamada Y., Hirai M., Udaka S.
    Biosci. Biotechnol. Biochem. 58:1424-1429(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: KASI.

Entry informationi

Entry nameiPDI_HUMIN
AccessioniPrimary (citable) accession number: P55059
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi