ID   APOC4_HUMAN             Reviewed;         127 AA.
AC   P55056; B3KWY6; Q53YY8;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   24-JAN-2024, entry version 176.
DE   RecName: Full=Apolipoprotein C-IV;
DE            Short=Apo-CIV;
DE            Short=ApoC-IV;
DE   AltName: Full=Apolipoprotein C4;
DE   Flags: Precursor;
GN   Name=APOC4;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8530039; DOI=10.1006/geno.1995.1144;
RA   Allan C.M., Walker D., Segrest J.P., Taylor J.M.;
RT   "Identification and characterization of a new human gene (APOC4) in the
RT   apolipoprotein E, C-I, and C-II gene locus.";
RL   Genomics 28:291-300(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT PRO-36.
RA   Nickerson D.A., Smith J.D., Fullerton S.M., Clark A.G., Stengard J.H.,
RA   Salomaa V., Boerwinkle E., Sing C.F., Weiss K.M.;
RL   Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Liver;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-63.
RC   TISSUE=Plasma;
RX   PubMed=16335952; DOI=10.1021/pr0502065;
RA   Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J.,
RA   Smith R.D.;
RT   "Human plasma N-glycoproteome analysis by immunoaffinity subtraction,
RT   hydrazide chemistry, and mass spectrometry.";
RL   J. Proteome Res. 4:2070-2080(2005).
RN   [7]
RP   VARIANTS PRO-36; ASP-52 AND ARG-96.
RX   PubMed=10996355; DOI=10.1016/s0021-9150(99)00459-1;
RA   Kamboh M.I., Aston C.E., Hamman R.F.;
RT   "DNA sequence variation in human apolipoprotein C4 gene and its effect on
RT   plasma lipid profile.";
RL   Atherosclerosis 152:193-201(2000).
RN   [8]
RP   VARIANT [LARGE SCALE ANALYSIS] GLN-75.
RX   PubMed=16959974; DOI=10.1126/science.1133427;
RA   Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA   Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA   Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA   Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA   Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA   Velculescu V.E.;
RT   "The consensus coding sequences of human breast and colorectal cancers.";
RL   Science 314:268-274(2006).
CC   -!- FUNCTION: May participate in lipoprotein metabolism.
CC   -!- INTERACTION:
CC       P55056; Q9BQE5: APOL2; NbExp=3; IntAct=EBI-18302142, EBI-4290634;
CC       P55056; Q92843: BCL2L2; NbExp=3; IntAct=EBI-18302142, EBI-707714;
CC       P55056; Q9NR28: DIABLO; NbExp=3; IntAct=EBI-18302142, EBI-517508;
CC       P55056; Q05329: GAD2; NbExp=3; IntAct=EBI-18302142, EBI-9304251;
CC       P55056; Q96QA5: GSDMA; NbExp=3; IntAct=EBI-18302142, EBI-11320924;
CC       P55056; P40305-1: IFI27; NbExp=3; IntAct=EBI-18302142, EBI-23832675;
CC       P55056; Q6P1Q0: LETMD1; NbExp=3; IntAct=EBI-18302142, EBI-1549822;
CC       P55056; Q8WWC4: MAIP1; NbExp=3; IntAct=EBI-18302142, EBI-1042937;
CC       P55056; Q5TGZ0: MICOS10; NbExp=3; IntAct=EBI-18302142, EBI-12886442;
CC       P55056; Q5XKP0: MICOS13; NbExp=3; IntAct=EBI-18302142, EBI-1053887;
CC       P55056; Q9UMS0: NFU1; NbExp=3; IntAct=EBI-18302142, EBI-725252;
CC       P55056; Q9H237-2: PORCN; NbExp=3; IntAct=EBI-18302142, EBI-18254170;
CC       P55056; Q8WXG1: RSAD2; NbExp=3; IntAct=EBI-18302142, EBI-12736320;
CC       P55056; Q9HC62: SENP2; NbExp=3; IntAct=EBI-18302142, EBI-714881;
CC       P55056; Q5SQN1: SNAP47; NbExp=3; IntAct=EBI-18302142, EBI-10244848;
CC       P55056; Q17RD7: SYT16; NbExp=3; IntAct=EBI-18302142, EBI-10238936;
CC       P55056; Q96BZ9: TBC1D20; NbExp=3; IntAct=EBI-18302142, EBI-9254454;
CC       P55056; P07204: THBD; NbExp=3; IntAct=EBI-18302142, EBI-941422;
CC       P55056; Q9NPL8: TIMMDC1; NbExp=3; IntAct=EBI-18302142, EBI-6268651;
CC       P55056; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-18302142, EBI-8638294;
CC       P55056; Q6ZUI0: TPRG1; NbExp=3; IntAct=EBI-18302142, EBI-17249488;
CC       P55056; Q9UMX0: UBQLN1; NbExp=3; IntAct=EBI-18302142, EBI-741480;
CC       P55056; Q9UHD9: UBQLN2; NbExp=3; IntAct=EBI-18302142, EBI-947187;
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the liver and secreted in plasma.
CC   -!- SIMILARITY: Belongs to the apolipoprotein C4 family. {ECO:0000305}.
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DR   EMBL; U32576; AAA91653.1; -; Genomic_DNA.
DR   EMBL; AY422953; AAQ91812.1; -; Genomic_DNA.
DR   EMBL; AK126257; BAG54298.1; -; mRNA.
DR   EMBL; CH471126; EAW57310.1; -; Genomic_DNA.
DR   EMBL; BC020723; AAH20723.1; -; mRNA.
DR   CCDS; CCDS12649.1; -.
DR   RefSeq; NP_001637.1; NM_001646.2.
DR   AlphaFoldDB; P55056; -.
DR   BioGRID; 106843; 29.
DR   IntAct; P55056; 23.
DR   STRING; 9606.ENSP00000468236; -.
DR   GlyConnect; 2021; 1 N-Linked glycan (1 site).
DR   GlyCosmos; P55056; 1 site, 2 glycans.
DR   GlyGen; P55056; 1 site, 2 N-linked glycans (1 site).
DR   iPTMnet; P55056; -.
DR   PhosphoSitePlus; P55056; -.
DR   BioMuta; APOC4; -.
DR   DMDM; 1703333; -.
DR   CPTAC; non-CPTAC-2626; -.
DR   jPOST; P55056; -.
DR   MassIVE; P55056; -.
DR   PaxDb; 9606-ENSP00000468236; -.
DR   PeptideAtlas; P55056; -.
DR   ProteomicsDB; 56768; -.
DR   Antibodypedia; 73673; 152 antibodies from 23 providers.
DR   DNASU; 346; -.
DR   Ensembl; ENST00000592954.2; ENSP00000468236.1; ENSG00000267467.4.
DR   GeneID; 346; -.
DR   KEGG; hsa:346; -.
DR   MANE-Select; ENST00000592954.2; ENSP00000468236.1; NM_001646.3; NP_001637.1.
DR   UCSC; uc060zuq.1; human.
DR   AGR; HGNC:611; -.
DR   CTD; 346; -.
DR   DisGeNET; 346; -.
DR   GeneCards; APOC4; -.
DR   HGNC; HGNC:611; APOC4.
DR   HPA; ENSG00000267467; Tissue enriched (liver).
DR   MIM; 600745; gene.
DR   neXtProt; NX_P55056; -.
DR   OpenTargets; ENSG00000224916; -.
DR   OpenTargets; ENSG00000267467; -.
DR   PharmGKB; PA54; -.
DR   VEuPathDB; HostDB:ENSG00000267467; -.
DR   eggNOG; ENOG502TE52; Eukaryota.
DR   GeneTree; ENSGT00390000015914; -.
DR   HOGENOM; CLU_161459_0_0_1; -.
DR   InParanoid; P55056; -.
DR   OMA; KWQWFWG; -.
DR   OrthoDB; 4638429at2759; -.
DR   PhylomeDB; P55056; -.
DR   TreeFam; TF336879; -.
DR   PathwayCommons; P55056; -.
DR   Reactome; R-HSA-8866423; VLDL assembly.
DR   Reactome; R-HSA-8964046; VLDL clearance.
DR   Reactome; R-HSA-9029569; NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux.
DR   SignaLink; P55056; -.
DR   BioGRID-ORCS; 346; 8 hits in 1130 CRISPR screens.
DR   GeneWiki; Apolipoprotein_C4; -.
DR   GenomeRNAi; 346; -.
DR   Pharos; P55056; Tbio.
DR   PRO; PR:P55056; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; P55056; Protein.
DR   Bgee; ENSG00000267467; Expressed in right lobe of liver and 83 other cell types or tissues.
DR   ExpressionAtlas; P55056; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR   GO; GO:0034364; C:high-density lipoprotein particle; IDA:BHF-UCL.
DR   GO; GO:0034361; C:very-low-density lipoprotein particle; IDA:BHF-UCL.
DR   GO; GO:0005319; F:lipid transporter activity; TAS:ProtInc.
DR   GO; GO:0006629; P:lipid metabolic process; TAS:ProtInc.
DR   GO; GO:0010890; P:positive regulation of sequestering of triglyceride; IMP:BHF-UCL.
DR   GO; GO:0070328; P:triglyceride homeostasis; IMP:BHF-UCL.
DR   InterPro; IPR028120; APOC4.
DR   PANTHER; PTHR32288; APOLIPOPROTEIN C-IV; 1.
DR   PANTHER; PTHR32288:SF0; APOLIPOPROTEIN C-IV; 1.
DR   Pfam; PF15119; APOC4; 1.
DR   Genevisible; P55056; HS.
PE   1: Evidence at protein level;
KW   Glycoprotein; Lipid transport; Reference proteome; Secreted; Signal;
KW   Transport.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000250|UniProtKB:P55057"
FT   CHAIN           28..127
FT                   /note="Apolipoprotein C-IV"
FT                   /id="PRO_0000002036"
FT   CARBOHYD        63
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:16335952"
FT   VARIANT         36
FT                   /note="L -> P (in dbSNP:rs1132899)"
FT                   /evidence="ECO:0000269|PubMed:10996355, ECO:0000269|Ref.2"
FT                   /id="VAR_012081"
FT   VARIANT         52
FT                   /note="G -> D (in dbSNP:rs12691089)"
FT                   /evidence="ECO:0000269|PubMed:10996355"
FT                   /id="VAR_012082"
FT   VARIANT         75
FT                   /note="P -> Q (in a breast cancer sample; somatic
FT                   mutation)"
FT                   /evidence="ECO:0000269|PubMed:16959974"
FT                   /id="VAR_036540"
FT   VARIANT         96
FT                   /note="L -> R (in dbSNP:rs5167)"
FT                   /evidence="ECO:0000269|PubMed:10996355"
FT                   /id="VAR_012068"
FT   VARIANT         126
FT                   /note="Q -> L (in dbSNP:rs5168)"
FT                   /id="VAR_012069"
SQ   SEQUENCE   127 AA;  14553 MW;  D7C7F6F2CD922775 CRC64;
     MSLLRNRLQA LPALCLCVLV LACIGACQPE AQEGTLSPPP KLKMSRWSLV RGRMKELLET
     VVNRTRDGWQ WFWSPSTFRG FMQTYYDDHL RDLGPLTKAW FLESKDSLLK KTHSLCPRLV
     CGDKDQG
//