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P55042 (RAD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP-binding protein RAD
Alternative name(s):
RAD1
Ras associated with diabetes
Gene names
Name:RRAD
Synonyms:RAD
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca2+ currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane By similarity. Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D. Ref.4

Subunit structure

Interacts with calmodulin preferentially in the inactive, GDP-bound form. Binds CAMKII which is capable of phosphorylating RAD in vitro. Interacts with CAMK2D and CACNB2. Interaction with CACNB2 regulates the trafficking of CACNA1C to the cell membrane By similarity. Ref.3 Ref.4

Subcellular location

Cell membrane By similarity.

Tissue specificity

Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans. Ref.4

Induction

Down-regulated in failing hearts. Ref.4

Sequence similarities

Belongs to the small GTPase superfamily. RGK family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   LigandCalmodulin-binding
GTP-binding
Nucleotide-binding
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processsmall GTPase mediated signal transduction

Traceable author statement Ref.1. Source: ProtInc

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CCNDBP1O952735EBI-3911502,EBI-748961

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308GTP-binding protein RAD
PRO_0000122478

Regions

Nucleotide binding98 – 1058GTP By similarity
Nucleotide binding203 – 2064GTP By similarity
Region278 – 29720Calmodulin-binding

Natural variations

Natural variant661Q → P.
Corresponds to variant rs7198458 [ dbSNP | Ensembl ].
VAR_049497

Secondary structure

........................... 308
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P55042 [UniParc].

Last modified November 7, 2003. Version 2.
Checksum: EF098960A371D2C4

FASTA30833,245
        10         20         30         40         50         60 
MTLNGGGSGA GGSRGGGQER ERRRGSTPWG PAPPLHRRSM PVDERDLQAA LTPGALTAAA 

        70         80         90        100        110        120 
AGTGTQGPRL DWPEDSEDSL SSGGSDSDES VYKVLLLGAP GVGKSALARI FGGVEDGPEA 

       130        140        150        160        170        180 
EAAGHTYDRS IVVDGEEASL MVYDIWEQDG GRWLPGHCMA MGDAYVIVYS VTDKGSFEKA 

       190        200        210        220        230        240 
SELRVQLRRA RQTDDVPIIL VGNKSDLVRS REVSVDEGRA CAVVFDCKFI ETSAALHHNV 

       250        260        270        280        290        300 
QALFEGVVRQ IRLRRDSKEA NARRQAGTRR RESLGKKAKR FLGRIVARNS RKMAFRAKSK 


SCHDLSVL 

« Hide

References

« Hide 'large scale' references
[1]"Rad: a member of the Ras family overexpressed in muscle of type II diabetic humans."
Reynet C., Kahn C.R.
Science 262:1441-1444(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Skeletal muscle.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta and Retina.
[3]"Rad and Rad-related GTPases interact with calmodulin and calmodulin-dependent protein kinase II."
Moyers J.S., Bilan P.J., Zhu J., Kahn C.R.
J. Biol. Chem. 272:11832-11839(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CALMODULIN.
[4]"Rad GTPase deficiency leads to cardiac hypertrophy."
Chang L., Zhang J., Tseng Y.-H., Xie C.-Q., Ilany J., Bruning J.C., Sun Z., Zhu X., Cui T., Youker K.A., Yang Q., Day S.M., Kahn C.R., Chen Y.E.
Circulation 116:2976-2983(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, INTERACTION WITH CAMK2D AND CALMODULIN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L24564 mRNA. Translation: AAA36540.1.
BC011645 mRNA. Translation: AAH11645.1.
BC057815 mRNA. Translation: AAH57815.1.
PIRA49334.
RefSeqNP_001122322.1. NM_001128850.1.
NP_004156.1. NM_004165.2.
UniGeneHs.1027.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DPXX-ray1.80A/B87-258[»]
2GJSX-ray1.90A/B87-260[»]
3Q72X-ray1.66A/B90-255[»]
3Q7PX-ray2.50A/B90-255[»]
3Q7QX-ray2.30A/B90-255[»]
ProteinModelPortalP55042.
SMRP55042. Positions 91-258.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112150. 11 interactions.
IntActP55042. 3 interactions.
STRING9606.ENSP00000299759.

PTM databases

PhosphoSiteP55042.

Polymorphism databases

DMDM38258885.

Proteomic databases

PaxDbP55042.
PRIDEP55042.

Protocols and materials databases

DNASU6236.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000299759; ENSP00000299759; ENSG00000166592.
ENST00000420652; ENSP00000388744; ENSG00000166592.
GeneID6236.
KEGGhsa:6236.
UCSCuc002eqn.2. human.

Organism-specific databases

CTD6236.
GeneCardsGC16M066955.
HGNCHGNC:10446. RRAD.
HPAHPA041755.
MIM179503. gene.
neXtProtNX_P55042.
PharmGKBPA34860.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000246961.
HOVERGENHBG104899.
InParanoidP55042.
KOK07845.
OMAEDNARRQ.
OrthoDBEOG7Q8CP8.
PhylomeDBP55042.
TreeFamTF314379.

Enzyme and pathway databases

SignaLinkP55042.

Gene expression databases

BgeeP55042.
CleanExHS_RRAD.
GenevestigatorP55042.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR028869. RRAD.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERPTHR24070. PTHR24070. 1 hit.
PTHR24070:SF74. PTHR24070:SF74. 1 hit.
PfamPF00071. Ras. 1 hit.
[Graphical view]
PIRSFPIRSF038017. GTP-binding_GEM. 1 hit.
PRINTSPR00449. RASTRNSFRMNG.
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP55042.
GeneWikiRRAD.
GenomeRNAi6236.
NextBio24211.
PROP55042.
SOURCESearch...

Entry information

Entry nameRAD_HUMAN
AccessionPrimary (citable) accession number: P55042
Secondary accession number(s): Q96F39
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 7, 2003
Last modified: April 16, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM