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Protein

GTP-binding protein RAD

Gene

RRAD

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca2+ currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi98 – 105GTPBy similarity8
Nucleotide bindingi203 – 206GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: ProtInc
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • small GTPase mediated signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000166592-MONOMER.
SignaLinkiP55042.
SIGNORiP55042.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein RAD
Alternative name(s):
RAD1
Ras associated with diabetes
Gene namesi
Name:RRAD
Synonyms:RAD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:10446. RRAD.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6236.
OpenTargetsiENSG00000166592.
PharmGKBiPA34860.

Polymorphism and mutation databases

BioMutaiRRAD.
DMDMi38258885.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224781 – 308GTP-binding protein RADAdd BLAST308

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24Omega-N-methylarginineBy similarity1
Modified residuei26PhosphoserineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiP55042.
PaxDbiP55042.
PeptideAtlasiP55042.
PRIDEiP55042.
TopDownProteomicsiP55042.

PTM databases

iPTMnetiP55042.
PhosphoSitePlusiP55042.

Expressioni

Tissue specificityi

Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.1 Publication

Inductioni

Down-regulated in failing hearts.1 Publication

Gene expression databases

BgeeiENSG00000166592.
CleanExiHS_RRAD.
ExpressionAtlasiP55042. baseline and differential.
GenevisibleiP55042. HS.

Organism-specific databases

HPAiHPA041755.

Interactioni

Subunit structurei

Interacts with calmodulin preferentially in the inactive, GDP-bound form. Binds CAMKII which is capable of phosphorylating RAD in vitro. Interacts with CAMK2D and CACNB2. Interaction with CACNB2 regulates the trafficking of CACNA1C to the cell membrane (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CCNDBP1O952735EBI-3911502,EBI-748961

Protein-protein interaction databases

BioGridi112150. 11 interactors.
IntActiP55042. 3 interactors.
STRINGi9606.ENSP00000299759.

Structurei

Secondary structure

1308
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi93 – 99Combined sources7
Helixi104 – 111Combined sources8
Beta strandi126 – 133Combined sources8
Beta strandi136 – 144Combined sources9
Helixi155 – 159Combined sources5
Beta strandi164 – 170Combined sources7
Helixi174 – 189Combined sources16
Beta strandi198 – 203Combined sources6
Helixi208 – 210Combined sources3
Helixi215 – 224Combined sources10
Beta strandi228 – 231Combined sources4
Helixi234 – 236Combined sources3
Helixi240 – 254Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DPXX-ray1.80A/B87-258[»]
2GJSX-ray1.90A/B87-260[»]
3Q72X-ray1.66A/B90-255[»]
3Q7PX-ray2.50A/B90-255[»]
3Q7QX-ray2.30A/B90-255[»]
ProteinModelPortaliP55042.
SMRiP55042.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55042.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni278 – 297Calmodulin-bindingAdd BLAST20

Sequence similaritiesi

Belongs to the small GTPase superfamily. RGK family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133672.
HOGENOMiHOG000246961.
HOVERGENiHBG104899.
InParanoidiP55042.
KOiK07845.
OMAiDRSIMVD.
OrthoDBiEOG091G0OCN.
PhylomeDBiP55042.
TreeFamiTF314379.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR028869. RRAD.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PTHR24070:SF74. PTHR24070:SF74. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55042-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLNGGGSGA GGSRGGGQER ERRRGSTPWG PAPPLHRRSM PVDERDLQAA
60 70 80 90 100
LTPGALTAAA AGTGTQGPRL DWPEDSEDSL SSGGSDSDES VYKVLLLGAP
110 120 130 140 150
GVGKSALARI FGGVEDGPEA EAAGHTYDRS IVVDGEEASL MVYDIWEQDG
160 170 180 190 200
GRWLPGHCMA MGDAYVIVYS VTDKGSFEKA SELRVQLRRA RQTDDVPIIL
210 220 230 240 250
VGNKSDLVRS REVSVDEGRA CAVVFDCKFI ETSAALHHNV QALFEGVVRQ
260 270 280 290 300
IRLRRDSKEA NARRQAGTRR RESLGKKAKR FLGRIVARNS RKMAFRAKSK

SCHDLSVL
Length:308
Mass (Da):33,245
Last modified:November 7, 2003 - v2
Checksum:iEF098960A371D2C4
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04949766Q → P.Corresponds to variant rs7198458dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24564 mRNA. Translation: AAA36540.1.
BC011645 mRNA. Translation: AAH11645.1.
BC057815 mRNA. Translation: AAH57815.1.
CCDSiCCDS10824.1.
PIRiA49334.
RefSeqiNP_001122322.1. NM_001128850.1.
NP_004156.1. NM_004165.2.
UniGeneiHs.1027.

Genome annotation databases

EnsembliENST00000299759; ENSP00000299759; ENSG00000166592.
ENST00000420652; ENSP00000388744; ENSG00000166592.
GeneIDi6236.
KEGGihsa:6236.
UCSCiuc002eqn.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24564 mRNA. Translation: AAA36540.1.
BC011645 mRNA. Translation: AAH11645.1.
BC057815 mRNA. Translation: AAH57815.1.
CCDSiCCDS10824.1.
PIRiA49334.
RefSeqiNP_001122322.1. NM_001128850.1.
NP_004156.1. NM_004165.2.
UniGeneiHs.1027.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DPXX-ray1.80A/B87-258[»]
2GJSX-ray1.90A/B87-260[»]
3Q72X-ray1.66A/B90-255[»]
3Q7PX-ray2.50A/B90-255[»]
3Q7QX-ray2.30A/B90-255[»]
ProteinModelPortaliP55042.
SMRiP55042.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112150. 11 interactors.
IntActiP55042. 3 interactors.
STRINGi9606.ENSP00000299759.

PTM databases

iPTMnetiP55042.
PhosphoSitePlusiP55042.

Polymorphism and mutation databases

BioMutaiRRAD.
DMDMi38258885.

Proteomic databases

MaxQBiP55042.
PaxDbiP55042.
PeptideAtlasiP55042.
PRIDEiP55042.
TopDownProteomicsiP55042.

Protocols and materials databases

DNASUi6236.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299759; ENSP00000299759; ENSG00000166592.
ENST00000420652; ENSP00000388744; ENSG00000166592.
GeneIDi6236.
KEGGihsa:6236.
UCSCiuc002eqn.2. human.

Organism-specific databases

CTDi6236.
DisGeNETi6236.
GeneCardsiRRAD.
HGNCiHGNC:10446. RRAD.
HPAiHPA041755.
MIMi179503. gene.
neXtProtiNX_P55042.
OpenTargetsiENSG00000166592.
PharmGKBiPA34860.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133672.
HOGENOMiHOG000246961.
HOVERGENiHBG104899.
InParanoidiP55042.
KOiK07845.
OMAiDRSIMVD.
OrthoDBiEOG091G0OCN.
PhylomeDBiP55042.
TreeFamiTF314379.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000166592-MONOMER.
SignaLinkiP55042.
SIGNORiP55042.

Miscellaneous databases

EvolutionaryTraceiP55042.
GeneWikiiRRAD.
GenomeRNAii6236.
PROiP55042.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166592.
CleanExiHS_RRAD.
ExpressionAtlasiP55042. baseline and differential.
GenevisibleiP55042. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR028869. RRAD.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PTHR24070:SF74. PTHR24070:SF74. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAD_HUMAN
AccessioniPrimary (citable) accession number: P55042
Secondary accession number(s): Q96F39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 7, 2003
Last modified: November 30, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.