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P55042

- RAD_HUMAN

UniProt

P55042 - RAD_HUMAN

Protein

GTP-binding protein RAD

Gene

RRAD

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (07 Nov 2003)
      Previous versions | rss
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    Functioni

    May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca2+ currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane By similarity. Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.By similarity1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi98 – 1058GTPBy similarity
    Nucleotide bindingi203 – 2064GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: ProtInc
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. small GTPase mediated signal transduction Source: ProtInc

    Keywords - Ligandi

    Calmodulin-binding, GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiP55042.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP-binding protein RAD
    Alternative name(s):
    RAD1
    Ras associated with diabetes
    Gene namesi
    Name:RRAD
    Synonyms:RAD
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:10446. RRAD.

    Subcellular locationi

    Cell membrane By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34860.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 308308GTP-binding protein RADPRO_0000122478Add
    BLAST

    Proteomic databases

    MaxQBiP55042.
    PaxDbiP55042.
    PRIDEiP55042.

    PTM databases

    PhosphoSiteiP55042.

    Expressioni

    Tissue specificityi

    Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.1 Publication

    Inductioni

    Down-regulated in failing hearts.1 Publication

    Gene expression databases

    BgeeiP55042.
    CleanExiHS_RRAD.
    GenevestigatoriP55042.

    Organism-specific databases

    HPAiHPA041755.

    Interactioni

    Subunit structurei

    Interacts with calmodulin preferentially in the inactive, GDP-bound form. Binds CAMKII which is capable of phosphorylating RAD in vitro. Interacts with CAMK2D and CACNB2. Interaction with CACNB2 regulates the trafficking of CACNA1C to the cell membrane By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CCNDBP1O952735EBI-3911502,EBI-748961

    Protein-protein interaction databases

    BioGridi112150. 11 interactions.
    IntActiP55042. 3 interactions.
    STRINGi9606.ENSP00000299759.

    Structurei

    Secondary structure

    1
    308
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi93 – 997
    Helixi104 – 1118
    Beta strandi126 – 1338
    Beta strandi136 – 1449
    Helixi155 – 1595
    Beta strandi164 – 1707
    Helixi174 – 18916
    Beta strandi198 – 2036
    Helixi208 – 2103
    Helixi215 – 22410
    Beta strandi228 – 2314
    Helixi234 – 2363
    Helixi240 – 25415

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DPXX-ray1.80A/B87-258[»]
    2GJSX-ray1.90A/B87-260[»]
    3Q72X-ray1.66A/B90-255[»]
    3Q7PX-ray2.50A/B90-255[»]
    3Q7QX-ray2.30A/B90-255[»]
    ProteinModelPortaliP55042.
    SMRiP55042. Positions 91-258.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP55042.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni278 – 29720Calmodulin-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. RGK family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000246961.
    HOVERGENiHBG104899.
    InParanoidiP55042.
    KOiK07845.
    OMAiGGIEDGP.
    OrthoDBiEOG7Q8CP8.
    PhylomeDBiP55042.
    TreeFamiTF314379.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR028869. RRAD.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR017358. Small_GTPase_GEM/REM/Rad.
    IPR020849. Small_GTPase_Ras.
    [Graphical view]
    PANTHERiPTHR24070. PTHR24070. 1 hit.
    PTHR24070:SF74. PTHR24070:SF74. 1 hit.
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
    PRINTSiPR00449. RASTRNSFRMNG.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51421. RAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P55042-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLNGGGSGA GGSRGGGQER ERRRGSTPWG PAPPLHRRSM PVDERDLQAA    50
    LTPGALTAAA AGTGTQGPRL DWPEDSEDSL SSGGSDSDES VYKVLLLGAP 100
    GVGKSALARI FGGVEDGPEA EAAGHTYDRS IVVDGEEASL MVYDIWEQDG 150
    GRWLPGHCMA MGDAYVIVYS VTDKGSFEKA SELRVQLRRA RQTDDVPIIL 200
    VGNKSDLVRS REVSVDEGRA CAVVFDCKFI ETSAALHHNV QALFEGVVRQ 250
    IRLRRDSKEA NARRQAGTRR RESLGKKAKR FLGRIVARNS RKMAFRAKSK 300
    SCHDLSVL 308
    Length:308
    Mass (Da):33,245
    Last modified:November 7, 2003 - v2
    Checksum:iEF098960A371D2C4
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti66 – 661Q → P.
    Corresponds to variant rs7198458 [ dbSNP | Ensembl ].
    VAR_049497

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24564 mRNA. Translation: AAA36540.1.
    BC011645 mRNA. Translation: AAH11645.1.
    BC057815 mRNA. Translation: AAH57815.1.
    CCDSiCCDS10824.1.
    PIRiA49334.
    RefSeqiNP_001122322.1. NM_001128850.1.
    NP_004156.1. NM_004165.2.
    UniGeneiHs.1027.

    Genome annotation databases

    EnsembliENST00000299759; ENSP00000299759; ENSG00000166592.
    ENST00000420652; ENSP00000388744; ENSG00000166592.
    GeneIDi6236.
    KEGGihsa:6236.
    UCSCiuc002eqn.2. human.

    Polymorphism databases

    DMDMi38258885.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24564 mRNA. Translation: AAA36540.1 .
    BC011645 mRNA. Translation: AAH11645.1 .
    BC057815 mRNA. Translation: AAH57815.1 .
    CCDSi CCDS10824.1.
    PIRi A49334.
    RefSeqi NP_001122322.1. NM_001128850.1.
    NP_004156.1. NM_004165.2.
    UniGenei Hs.1027.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DPX X-ray 1.80 A/B 87-258 [» ]
    2GJS X-ray 1.90 A/B 87-260 [» ]
    3Q72 X-ray 1.66 A/B 90-255 [» ]
    3Q7P X-ray 2.50 A/B 90-255 [» ]
    3Q7Q X-ray 2.30 A/B 90-255 [» ]
    ProteinModelPortali P55042.
    SMRi P55042. Positions 91-258.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112150. 11 interactions.
    IntActi P55042. 3 interactions.
    STRINGi 9606.ENSP00000299759.

    PTM databases

    PhosphoSitei P55042.

    Polymorphism databases

    DMDMi 38258885.

    Proteomic databases

    MaxQBi P55042.
    PaxDbi P55042.
    PRIDEi P55042.

    Protocols and materials databases

    DNASUi 6236.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000299759 ; ENSP00000299759 ; ENSG00000166592 .
    ENST00000420652 ; ENSP00000388744 ; ENSG00000166592 .
    GeneIDi 6236.
    KEGGi hsa:6236.
    UCSCi uc002eqn.2. human.

    Organism-specific databases

    CTDi 6236.
    GeneCardsi GC16M066955.
    HGNCi HGNC:10446. RRAD.
    HPAi HPA041755.
    MIMi 179503. gene.
    neXtProti NX_P55042.
    PharmGKBi PA34860.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000246961.
    HOVERGENi HBG104899.
    InParanoidi P55042.
    KOi K07845.
    OMAi GGIEDGP.
    OrthoDBi EOG7Q8CP8.
    PhylomeDBi P55042.
    TreeFami TF314379.

    Enzyme and pathway databases

    SignaLinki P55042.

    Miscellaneous databases

    EvolutionaryTracei P55042.
    GeneWikii RRAD.
    GenomeRNAii 6236.
    NextBioi 24211.
    PROi P55042.
    SOURCEi Search...

    Gene expression databases

    Bgeei P55042.
    CleanExi HS_RRAD.
    Genevestigatori P55042.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR028869. RRAD.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR017358. Small_GTPase_GEM/REM/Rad.
    IPR020849. Small_GTPase_Ras.
    [Graphical view ]
    PANTHERi PTHR24070. PTHR24070. 1 hit.
    PTHR24070:SF74. PTHR24070:SF74. 1 hit.
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038017. GTP-binding_GEM. 1 hit.
    PRINTSi PR00449. RASTRNSFRMNG.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51421. RAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rad: a member of the Ras family overexpressed in muscle of type II diabetic humans."
      Reynet C., Kahn C.R.
      Science 262:1441-1444(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Skeletal muscle.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta and Retina.
    3. "Rad and Rad-related GTPases interact with calmodulin and calmodulin-dependent protein kinase II."
      Moyers J.S., Bilan P.J., Zhu J., Kahn C.R.
      J. Biol. Chem. 272:11832-11839(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CALMODULIN.
    4. Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, INTERACTION WITH CAMK2D AND CALMODULIN.

    Entry informationi

    Entry nameiRAD_HUMAN
    AccessioniPrimary (citable) accession number: P55042
    Secondary accession number(s): Q96F39
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: November 7, 2003
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3