GTP-binding protein RAD - Homo sapiens (Human)

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P55042 (RAD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 118. History...

Clusters with 100%, 90%, 50% identity |

Documents (6) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GTP-binding protein RAD

Alternative name(s):
RAD1
Ras associated with diabetes

Gene names

Name:	RRAD
Synonyms:	RAD

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca²⁺ currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane By similarity. Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D. Ref.4
Subunit structure	Interacts with calmodulin preferentially in the inactive, GDP-bound form. Binds CAMKII which is capable of phosphorylating RAD in vitro. Interacts with CAMK2D and CACNB2. Interaction with CACNB2 regulates the trafficking of CACNA1C to the cell membrane By similarity. Ref.3 Ref.4
Subcellular location	Cell membrane By similarity.
Tissue specificity	Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans. Ref.4
Induction	Down-regulated in failing hearts. Ref.4
Sequence similarities	Belongs to the small GTPase superfamily. RGK family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Ligand	Calmodulin-binding GTP-binding Nucleotide-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	small GTPase mediated signal transduction Traceable author statement Ref.1. Source: ProtInc
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CCNDBP1	O95273	5	EBI-3911502,EBI-748961

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 308

308

GTP-binding protein RAD

PRO_0000122478

Regions

Nucleotide binding

98 – 105

GTP By similarity

Nucleotide binding

203 – 206

GTP By similarity

Region

278 – 297

Calmodulin-binding

Natural variations

Natural variant

Q → P.
Corresponds to variant rs7198458 [ dbSNP | Ensembl ].

VAR_049497

Secondary structure

308

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P55042 [UniParc].

Last modified November 7, 2003. Version 2.
Checksum: EF098960A371D2C4
Show »

FASTA

308

33,245

        10         20         30         40         50         60 
MTLNGGGSGA GGSRGGGQER ERRRGSTPWG PAPPLHRRSM PVDERDLQAA LTPGALTAAA 

        70         80         90        100        110        120 
AGTGTQGPRL DWPEDSEDSL SSGGSDSDES VYKVLLLGAP GVGKSALARI FGGVEDGPEA 

       130        140        150        160        170        180 
EAAGHTYDRS IVVDGEEASL MVYDIWEQDG GRWLPGHCMA MGDAYVIVYS VTDKGSFEKA 

       190        200        210        220        230        240 
SELRVQLRRA RQTDDVPIIL VGNKSDLVRS REVSVDEGRA CAVVFDCKFI ETSAALHHNV 

       250        260        270        280        290        300 
QALFEGVVRQ IRLRRDSKEA NARRQAGTRR RESLGKKAKR FLGRIVARNS RKMAFRAKSK 


SCHDLSVL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rad: a member of the Ras family overexpressed in muscle of type II diabetic humans." Reynet C., Kahn C.R. Science 262:1441-1444(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta and Retina.
[3]	"Rad and Rad-related GTPases interact with calmodulin and calmodulin-dependent protein kinase II." Moyers J.S., Bilan P.J., Zhu J., Kahn C.R. J. Biol. Chem. 272:11832-11839(1997) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CALMODULIN.
[4]	"Rad GTPase deficiency leads to cardiac hypertrophy." Chang L., Zhang J., Tseng Y.-H., Xie C.-Q., Ilany J., Bruning J.C., Sun Z., Zhu X., Cui T., Youker K.A., Yang Q., Day S.M., Kahn C.R., Chen Y.E. Circulation 116:2976-2983(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, INTERACTION WITH CAMK2D AND CALMODULIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L24564 mRNA. Translation: AAA36540.1.
BC011645 mRNA. Translation: AAH11645.1.
BC057815 mRNA. Translation: AAH57815.1.

IPI

IPI00375333.

PIR

A49334.

RefSeq

NP_001122322.1. NM_001128850.1.
NP_004156.1. NM_004165.2.

UniGene

Hs.1027.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DPX	X-ray	1.80	A/B	87-258	[»]
2GJS	X-ray	1.90	A/B	87-260	[»]
3Q72	X-ray	1.66	A/B	90-255	[»]
3Q7P	X-ray	2.50	A/B	90-255	[»]
3Q7Q	X-ray	2.30	A/B	90-255	[»]

ProteinModelPortal

P55042.

SMR

P55042. Positions 91-258.

ModBase

Search...

Protein-protein interaction databases

IntAct

P55042. 3 interactions.

STRING

9606.ENSP00000299759.

PTM databases

PhosphoSite

P55042.

Polymorphism databases

DMDM

38258885.

Proteomic databases

PaxDb

P55042.

PRIDE

P55042.

Protocols and materials databases

DNASU

6236.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000299759; ENSP00000299759; ENSG00000166592.
ENST00000420652; ENSP00000388744; ENSG00000166592.

GeneID

6236.

KEGG

hsa:6236.

UCSC

uc002eqn.2. human.

Organism-specific databases

CTD

6236.

GeneCards

GC16M066955.

HGNC

HGNC:10446. RRAD.

HPA

HPA041755.

MIM

179503. gene.

neXtProt

NX_P55042.

PharmGKB

PA34860.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1100.

HOGENOM

HOG000246961.

HOVERGEN

HBG104899.

InParanoid

P55042.

K07845.

OMA

EDNARRQ.

OrthoDB

EOG4WSWB3.

PhylomeDB

P55042.

Gene expression databases

Bgee

P55042.

CleanEx

HS_RRAD.

Genevestigator

P55042.

GermOnline

ENSG00000166592. Homo sapiens.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]

PANTHER

PTHR24070. PTHR24070. 1 hit.

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PIRSF

PIRSF038017. GTP-binding_GEM. 1 hit.

PRINTS

PR00449. RASTRNSFRMNG.

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51421. RAS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P55042.

GenomeRNAi

6236.

NextBio

24211.

SOURCE

Search...

Entry information

Entry name

RAD_HUMAN

Accession

Primary (citable) accession number: P55042
Secondary accession number(s): Q96F39

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 7, 2003
Last modified:	April 3, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents