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P55042

- RAD_HUMAN

UniProt

P55042 - RAD_HUMAN

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Protein

GTP-binding protein RAD

Gene

RRAD

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca2+ currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane By similarity. Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi98 – 1058GTPBy similarity
Nucleotide bindingi203 – 2064GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: ProtInc
  2. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. small GTPase mediated signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiP55042.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein RAD
Alternative name(s):
RAD1
Ras associated with diabetes
Gene namesi
Name:RRAD
Synonyms:RAD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:10446. RRAD.

Subcellular locationi

Cell membrane By similarity

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34860.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308GTP-binding protein RADPRO_0000122478Add
BLAST

Proteomic databases

MaxQBiP55042.
PaxDbiP55042.
PRIDEiP55042.

PTM databases

PhosphoSiteiP55042.

Expressioni

Tissue specificityi

Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.1 Publication

Inductioni

Down-regulated in failing hearts.1 Publication

Gene expression databases

BgeeiP55042.
CleanExiHS_RRAD.
GenevestigatoriP55042.

Organism-specific databases

HPAiHPA041755.

Interactioni

Subunit structurei

Interacts with calmodulin preferentially in the inactive, GDP-bound form. Binds CAMKII which is capable of phosphorylating RAD in vitro. Interacts with CAMK2D and CACNB2. Interaction with CACNB2 regulates the trafficking of CACNA1C to the cell membrane By similarity.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CCNDBP1O952735EBI-3911502,EBI-748961

Protein-protein interaction databases

BioGridi112150. 11 interactions.
IntActiP55042. 3 interactions.
STRINGi9606.ENSP00000299759.

Structurei

Secondary structure

1
308
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi93 – 997Combined sources
Helixi104 – 1118Combined sources
Beta strandi126 – 1338Combined sources
Beta strandi136 – 1449Combined sources
Helixi155 – 1595Combined sources
Beta strandi164 – 1707Combined sources
Helixi174 – 18916Combined sources
Beta strandi198 – 2036Combined sources
Helixi208 – 2103Combined sources
Helixi215 – 22410Combined sources
Beta strandi228 – 2314Combined sources
Helixi234 – 2363Combined sources
Helixi240 – 25415Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DPXX-ray1.80A/B87-258[»]
2GJSX-ray1.90A/B87-260[»]
3Q72X-ray1.66A/B90-255[»]
3Q7PX-ray2.50A/B90-255[»]
3Q7QX-ray2.30A/B90-255[»]
ProteinModelPortaliP55042.
SMRiP55042. Positions 91-258.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55042.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni278 – 29720Calmodulin-bindingAdd
BLAST

Sequence similaritiesi

Belongs to the small GTPase superfamily. RGK family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118909.
HOGENOMiHOG000246961.
HOVERGENiHBG104899.
InParanoidiP55042.
KOiK07845.
OMAiGGIEDGP.
OrthoDBiEOG7Q8CP8.
PhylomeDBiP55042.
TreeFamiTF314379.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR028869. RRAD.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PTHR24070:SF74. PTHR24070:SF74. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
PRINTSiPR00449. RASTRNSFRMNG.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55042-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLNGGGSGA GGSRGGGQER ERRRGSTPWG PAPPLHRRSM PVDERDLQAA
60 70 80 90 100
LTPGALTAAA AGTGTQGPRL DWPEDSEDSL SSGGSDSDES VYKVLLLGAP
110 120 130 140 150
GVGKSALARI FGGVEDGPEA EAAGHTYDRS IVVDGEEASL MVYDIWEQDG
160 170 180 190 200
GRWLPGHCMA MGDAYVIVYS VTDKGSFEKA SELRVQLRRA RQTDDVPIIL
210 220 230 240 250
VGNKSDLVRS REVSVDEGRA CAVVFDCKFI ETSAALHHNV QALFEGVVRQ
260 270 280 290 300
IRLRRDSKEA NARRQAGTRR RESLGKKAKR FLGRIVARNS RKMAFRAKSK

SCHDLSVL
Length:308
Mass (Da):33,245
Last modified:November 7, 2003 - v2
Checksum:iEF098960A371D2C4
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti66 – 661Q → P.
Corresponds to variant rs7198458 [ dbSNP | Ensembl ].
VAR_049497

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L24564 mRNA. Translation: AAA36540.1.
BC011645 mRNA. Translation: AAH11645.1.
BC057815 mRNA. Translation: AAH57815.1.
CCDSiCCDS10824.1.
PIRiA49334.
RefSeqiNP_001122322.1. NM_001128850.1.
NP_004156.1. NM_004165.2.
UniGeneiHs.1027.

Genome annotation databases

EnsembliENST00000299759; ENSP00000299759; ENSG00000166592.
ENST00000420652; ENSP00000388744; ENSG00000166592.
GeneIDi6236.
KEGGihsa:6236.
UCSCiuc002eqn.2. human.

Polymorphism databases

DMDMi38258885.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L24564 mRNA. Translation: AAA36540.1 .
BC011645 mRNA. Translation: AAH11645.1 .
BC057815 mRNA. Translation: AAH57815.1 .
CCDSi CCDS10824.1.
PIRi A49334.
RefSeqi NP_001122322.1. NM_001128850.1.
NP_004156.1. NM_004165.2.
UniGenei Hs.1027.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2DPX X-ray 1.80 A/B 87-258 [» ]
2GJS X-ray 1.90 A/B 87-260 [» ]
3Q72 X-ray 1.66 A/B 90-255 [» ]
3Q7P X-ray 2.50 A/B 90-255 [» ]
3Q7Q X-ray 2.30 A/B 90-255 [» ]
ProteinModelPortali P55042.
SMRi P55042. Positions 91-258.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112150. 11 interactions.
IntActi P55042. 3 interactions.
STRINGi 9606.ENSP00000299759.

PTM databases

PhosphoSitei P55042.

Polymorphism databases

DMDMi 38258885.

Proteomic databases

MaxQBi P55042.
PaxDbi P55042.
PRIDEi P55042.

Protocols and materials databases

DNASUi 6236.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000299759 ; ENSP00000299759 ; ENSG00000166592 .
ENST00000420652 ; ENSP00000388744 ; ENSG00000166592 .
GeneIDi 6236.
KEGGi hsa:6236.
UCSCi uc002eqn.2. human.

Organism-specific databases

CTDi 6236.
GeneCardsi GC16M066955.
HGNCi HGNC:10446. RRAD.
HPAi HPA041755.
MIMi 179503. gene.
neXtProti NX_P55042.
PharmGKBi PA34860.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000118909.
HOGENOMi HOG000246961.
HOVERGENi HBG104899.
InParanoidi P55042.
KOi K07845.
OMAi GGIEDGP.
OrthoDBi EOG7Q8CP8.
PhylomeDBi P55042.
TreeFami TF314379.

Enzyme and pathway databases

SignaLinki P55042.

Miscellaneous databases

EvolutionaryTracei P55042.
GeneWikii RRAD.
GenomeRNAii 6236.
NextBioi 24211.
PROi P55042.
SOURCEi Search...

Gene expression databases

Bgeei P55042.
CleanExi HS_RRAD.
Genevestigatori P55042.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR028869. RRAD.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view ]
PANTHERi PTHR24070. PTHR24070. 1 hit.
PTHR24070:SF74. PTHR24070:SF74. 1 hit.
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PIRSFi PIRSF038017. GTP-binding_GEM. 1 hit.
PRINTSi PR00449. RASTRNSFRMNG.
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51421. RAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rad: a member of the Ras family overexpressed in muscle of type II diabetic humans."
    Reynet C., Kahn C.R.
    Science 262:1441-1444(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta and Retina.
  3. "Rad and Rad-related GTPases interact with calmodulin and calmodulin-dependent protein kinase II."
    Moyers J.S., Bilan P.J., Zhu J., Kahn C.R.
    J. Biol. Chem. 272:11832-11839(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CALMODULIN.
  4. Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, INTERACTION WITH CAMK2D AND CALMODULIN.

Entry informationi

Entry nameiRAD_HUMAN
AccessioniPrimary (citable) accession number: P55042
Secondary accession number(s): Q96F39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 7, 2003
Last modified: October 29, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3