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P55041

- GEM_MOUSE

UniProt

P55041 - GEM_MOUSE

Protein

GTP-binding protein GEM

Gene

Gem

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (07 Nov 2003)
      Previous versions | rss
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    Functioni

    Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi81 – 888GTPBy similarity
    Nucleotide bindingi190 – 1934GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: Ensembl
    2. GTP binding Source: UniProtKB-KW
    3. magnesium ion binding Source: Ensembl
    4. protein binding Source: IntAct

    GO - Biological processi

    1. chromosome organization Source: Ensembl
    2. GTP catabolic process Source: InterPro
    3. mitotic nuclear division Source: Ensembl
    4. small GTPase mediated signal transduction Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP-binding protein GEM
    Alternative name(s):
    GTP-binding mitogen-induced T-cell protein
    RAS-like protein KIR
    Gene namesi
    Name:Gem
    Synonyms:Kir
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:99844. Gem.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic side of plasma membrane Source: Ensembl
    2. midbody Source: Ensembl
    3. mitotic spindle Source: Ensembl
    4. nucleus Source: MGI
    5. spindle midzone Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi269 – 2691W → G: No calmodulin binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 295295GTP-binding protein GEMPRO_0000122476Add
    BLAST

    Post-translational modificationi

    Phosphorylated on tyrosine residues.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP55041.

    PTM databases

    PhosphoSiteiP55041.

    Expressioni

    Inductioni

    By mitogens.

    Gene expression databases

    ArrayExpressiP55041.
    BgeeiP55041.
    GenevestigatoriP55041.

    Interactioni

    Subunit structurei

    Interacts with calmodulin in a Ca2+-dependent manner. Calmodulin binding significantly decreases GTP binding. Binds ROCK1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    YWHABP319463EBI-7082069,EBI-359815From a different organism.

    Protein-protein interaction databases

    IntActiP55041. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP55041.
    SMRiP55041. Positions 72-243.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni265 – 28420Calmodulin-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. RGK family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000246961.
    HOVERGENiHBG104899.
    InParanoidiP55041.
    KOiK07846.
    OMAiAPEDHCR.
    OrthoDBiEOG7Q8CP8.
    PhylomeDBiP55041.
    TreeFamiTF314379.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR017358. Small_GTPase_GEM/REM/Rad.
    IPR020849. Small_GTPase_Ras.
    [Graphical view]
    PANTHERiPTHR24070. PTHR24070. 1 hit.
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
    PRINTSiPR00449. RASTRNSFRMNG.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51421. RAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P55041-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLNNVTMRQ GTVGMQPQQR WSIPADARHL MVQKDPHPCN LRNRHSTAPE    50
    EHCRRSWSSD STDSVISSES GNTYYRVVLI GEQGVGKSTL ANIFAGVHDS 100
    MDSDCEVLGE DTYERTLVVD GESATIILLD MWENKGENEW LHDHCMQVGD 150
    AYLIVYSITD RASFEKASEL RIQLRRARQT EDIPIILVGN KSDLVRCREV 200
    SVSEGRACAV VFDCKFIETS AAVQHNVKEL FEGIVRQVRL RRDSKEKNER 250
    RLAYQKRRES IPRKARRFWG KIVAKNNKNM AFKLKSKSCH DLSVL 295
    Length:295
    Mass (Da):33,725
    Last modified:November 7, 2003 - v2
    Checksum:i4618F225D4FAAFA5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti23 – 231I → M in AAA64912. (PubMed:7912851)Curated
    Sequence conflicti56 – 561S → T in AAA64912. (PubMed:7912851)Curated
    Sequence conflicti235 – 2351V → E in AAA64912. (PubMed:7912851)Curated
    Sequence conflicti241 – 2411R → P in AAA64912. (PubMed:7912851)Curated
    Sequence conflicti282 – 2843FKL → SSS in AAA64912. (PubMed:7912851)Curated
    Sequence conflicti294 – 2941V → W in AAC52145. (PubMed:7809057)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10551 mRNA. Translation: AAA64912.1.
    U13053 mRNA. Translation: AAC52145.1.
    BC029668 mRNA. Translation: AAH29668.1.
    CCDSiCCDS17971.1.
    PIRiB54575.
    I49117.
    RefSeqiNP_034406.2. NM_010276.4.
    UniGeneiMm.247486.

    Genome annotation databases

    EnsembliENSMUST00000029868; ENSMUSP00000029868; ENSMUSG00000028214.
    ENSMUST00000108304; ENSMUSP00000103939; ENSMUSG00000028214.
    GeneIDi14579.
    KEGGimmu:14579.
    UCSCiuc008rzv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10551 mRNA. Translation: AAA64912.1 .
    U13053 mRNA. Translation: AAC52145.1 .
    BC029668 mRNA. Translation: AAH29668.1 .
    CCDSi CCDS17971.1.
    PIRi B54575.
    I49117.
    RefSeqi NP_034406.2. NM_010276.4.
    UniGenei Mm.247486.

    3D structure databases

    ProteinModelPortali P55041.
    SMRi P55041. Positions 72-243.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P55041. 2 interactions.

    PTM databases

    PhosphoSitei P55041.

    Proteomic databases

    PRIDEi P55041.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029868 ; ENSMUSP00000029868 ; ENSMUSG00000028214 .
    ENSMUST00000108304 ; ENSMUSP00000103939 ; ENSMUSG00000028214 .
    GeneIDi 14579.
    KEGGi mmu:14579.
    UCSCi uc008rzv.2. mouse.

    Organism-specific databases

    CTDi 2669.
    MGIi MGI:99844. Gem.

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000246961.
    HOVERGENi HBG104899.
    InParanoidi P55041.
    KOi K07846.
    OMAi APEDHCR.
    OrthoDBi EOG7Q8CP8.
    PhylomeDBi P55041.
    TreeFami TF314379.

    Miscellaneous databases

    NextBioi 286302.
    PROi P55041.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P55041.
    Bgeei P55041.
    Genevestigatori P55041.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR017358. Small_GTPase_GEM/REM/Rad.
    IPR020849. Small_GTPase_Ras.
    [Graphical view ]
    PANTHERi PTHR24070. PTHR24070. 1 hit.
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038017. GTP-binding_GEM. 1 hit.
    PRINTSi PR00449. RASTRNSFRMNG.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51421. RAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gem: an induced, immediate early protein belonging to the Ras family."
      Maguire J., Santoro T., Jensen P., Siebenlist U., Yewdell J., Kelly K.
      Science 265:241-244(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Transcriptional activation of a ras-like gene (kir) by oncogenic tyrosine kinases."
      Cohen L., Mohr R., Chen Y.-Y., Huang M., Kato R., Dorin D., Tamanoi F., Goga A., Afar D., Rosenberg N., Witte O.
      Proc. Natl. Acad. Sci. U.S.A. 91:12448-12452(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Retina.
    4. "Calmodulin binds to and inhibits GTP binding of the ras-like GTPase Kir/Gem."
      Fischer R., Wei Y., Anagli J., Berchtold M.W.
      J. Biol. Chem. 271:25067-25070(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CALMODULIN, MUTAGENESIS OF TRP-269.

    Entry informationi

    Entry nameiGEM_MOUSE
    AccessioniPrimary (citable) accession number: P55041
    Secondary accession number(s): Q8JZS1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: November 7, 2003
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3