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Protein

GTP-binding protein GEM

Gene

Gem

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi81 – 888GTPBy similarity
Nucleotide bindingi190 – 1934GTPBy similarity

GO - Molecular functioni

  1. GDP binding Source: MGI
  2. GTPase activity Source: MGI
  3. GTP binding Source: MGI
  4. magnesium ion binding Source: MGI

GO - Biological processi

  1. chromosome organization Source: MGI
  2. metaphase plate congression Source: MGI
  3. mitotic nuclear division Source: MGI
  4. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein GEM
Alternative name(s):
GTP-binding mitogen-induced T-cell protein
RAS-like protein KIR
Gene namesi
Name:Gem
Synonyms:Kir
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:99844. Gem.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmic side of plasma membrane Source: MGI
  2. midbody Source: MGI
  3. mitotic spindle Source: MGI
  4. nucleus Source: MGI
  5. spindle midzone Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi269 – 2691W → G: No calmodulin binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295GTP-binding protein GEMPRO_0000122476Add
BLAST

Post-translational modificationi

Phosphorylated on tyrosine residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP55041.

PTM databases

PhosphoSiteiP55041.

Expressioni

Inductioni

By mitogens.

Gene expression databases

BgeeiP55041.
ExpressionAtlasiP55041. baseline and differential.
GenevestigatoriP55041.

Interactioni

Subunit structurei

Interacts with calmodulin in a Ca2+-dependent manner. Calmodulin binding significantly decreases GTP binding. Binds ROCK1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
YWHABP319463EBI-7082069,EBI-359815From a different organism.

Protein-protein interaction databases

IntActiP55041. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP55041.
SMRiP55041. Positions 72-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni265 – 28420Calmodulin-bindingAdd
BLAST

Sequence similaritiesi

Belongs to the small GTPase superfamily. RGK family.Curated

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000246961.
HOVERGENiHBG104899.
InParanoidiP55041.
KOiK07846.
OMAiAPEDHCR.
OrthoDBiEOG7Q8CP8.
PhylomeDBiP55041.
TreeFamiTF314379.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
PRINTSiPR00449. RASTRNSFRMNG.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55041-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLNNVTMRQ GTVGMQPQQR WSIPADARHL MVQKDPHPCN LRNRHSTAPE
60 70 80 90 100
EHCRRSWSSD STDSVISSES GNTYYRVVLI GEQGVGKSTL ANIFAGVHDS
110 120 130 140 150
MDSDCEVLGE DTYERTLVVD GESATIILLD MWENKGENEW LHDHCMQVGD
160 170 180 190 200
AYLIVYSITD RASFEKASEL RIQLRRARQT EDIPIILVGN KSDLVRCREV
210 220 230 240 250
SVSEGRACAV VFDCKFIETS AAVQHNVKEL FEGIVRQVRL RRDSKEKNER
260 270 280 290
RLAYQKRRES IPRKARRFWG KIVAKNNKNM AFKLKSKSCH DLSVL
Length:295
Mass (Da):33,725
Last modified:November 7, 2003 - v2
Checksum:i4618F225D4FAAFA5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231I → M in AAA64912. (PubMed:7912851)Curated
Sequence conflicti56 – 561S → T in AAA64912. (PubMed:7912851)Curated
Sequence conflicti235 – 2351V → E in AAA64912. (PubMed:7912851)Curated
Sequence conflicti241 – 2411R → P in AAA64912. (PubMed:7912851)Curated
Sequence conflicti282 – 2843FKL → SSS in AAA64912. (PubMed:7912851)Curated
Sequence conflicti294 – 2941V → W in AAC52145. (PubMed:7809057)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10551 mRNA. Translation: AAA64912.1.
U13053 mRNA. Translation: AAC52145.1.
BC029668 mRNA. Translation: AAH29668.1.
CCDSiCCDS17971.1.
PIRiB54575.
I49117.
RefSeqiNP_034406.2. NM_010276.4.
UniGeneiMm.247486.

Genome annotation databases

EnsembliENSMUST00000029868; ENSMUSP00000029868; ENSMUSG00000028214.
ENSMUST00000108304; ENSMUSP00000103939; ENSMUSG00000028214.
GeneIDi14579.
KEGGimmu:14579.
UCSCiuc008rzv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10551 mRNA. Translation: AAA64912.1.
U13053 mRNA. Translation: AAC52145.1.
BC029668 mRNA. Translation: AAH29668.1.
CCDSiCCDS17971.1.
PIRiB54575.
I49117.
RefSeqiNP_034406.2. NM_010276.4.
UniGeneiMm.247486.

3D structure databases

ProteinModelPortaliP55041.
SMRiP55041. Positions 72-243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP55041. 2 interactions.

PTM databases

PhosphoSiteiP55041.

Proteomic databases

PRIDEiP55041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029868; ENSMUSP00000029868; ENSMUSG00000028214.
ENSMUST00000108304; ENSMUSP00000103939; ENSMUSG00000028214.
GeneIDi14579.
KEGGimmu:14579.
UCSCiuc008rzv.2. mouse.

Organism-specific databases

CTDi2669.
MGIiMGI:99844. Gem.

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000246961.
HOVERGENiHBG104899.
InParanoidiP55041.
KOiK07846.
OMAiAPEDHCR.
OrthoDBiEOG7Q8CP8.
PhylomeDBiP55041.
TreeFamiTF314379.

Miscellaneous databases

NextBioi286302.
PROiP55041.
SOURCEiSearch...

Gene expression databases

BgeeiP55041.
ExpressionAtlasiP55041. baseline and differential.
GenevestigatoriP55041.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
PRINTSiPR00449. RASTRNSFRMNG.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gem: an induced, immediate early protein belonging to the Ras family."
    Maguire J., Santoro T., Jensen P., Siebenlist U., Yewdell J., Kelly K.
    Science 265:241-244(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Transcriptional activation of a ras-like gene (kir) by oncogenic tyrosine kinases."
    Cohen L., Mohr R., Chen Y.-Y., Huang M., Kato R., Dorin D., Tamanoi F., Goga A., Afar D., Rosenberg N., Witte O.
    Proc. Natl. Acad. Sci. U.S.A. 91:12448-12452(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.
  4. "Calmodulin binds to and inhibits GTP binding of the ras-like GTPase Kir/Gem."
    Fischer R., Wei Y., Anagli J., Berchtold M.W.
    J. Biol. Chem. 271:25067-25070(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CALMODULIN, MUTAGENESIS OF TRP-269.

Entry informationi

Entry nameiGEM_MOUSE
AccessioniPrimary (citable) accession number: P55041
Secondary accession number(s): Q8JZS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 7, 2003
Last modified: February 4, 2015
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.