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Protein

GTP-binding protein GEM

Gene

Gem

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi81 – 88GTPBy similarity8
Nucleotide bindingi190 – 193GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein GEM
Alternative name(s):
GTP-binding mitogen-induced T-cell protein
RAS-like protein KIR
Gene namesi
Name:Gem
Synonyms:Kir
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:99844. Gem.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic side of plasma membrane Source: MGI
  • midbody Source: MGI
  • mitotic spindle Source: MGI
  • nucleus Source: MGI
  • spindle midzone Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi269W → G: No calmodulin binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224761 – 295GTP-binding protein GEMAdd BLAST295

Post-translational modificationi

Phosphorylated on tyrosine residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP55041.
PaxDbiP55041.
PeptideAtlasiP55041.
PRIDEiP55041.

PTM databases

iPTMnetiP55041.
PhosphoSitePlusiP55041.

Expressioni

Inductioni

By mitogens.

Gene expression databases

BgeeiENSMUSG00000028214.
ExpressionAtlasiP55041. baseline and differential.
GenevisibleiP55041. MM.

Interactioni

Subunit structurei

Interacts with calmodulin in a Ca2+-dependent manner. Calmodulin binding significantly decreases GTP binding. Binds ROCK1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
YWHABP319463EBI-7082069,EBI-359815From a different organism.

Protein-protein interaction databases

IntActiP55041. 2 interactors.
STRINGi10090.ENSMUSP00000029868.

Structurei

3D structure databases

ProteinModelPortaliP55041.
SMRiP55041.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 284Calmodulin-bindingAdd BLAST20

Sequence similaritiesi

Belongs to the small GTPase superfamily. RGK family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133672.
HOGENOMiHOG000246961.
HOVERGENiHBG104899.
InParanoidiP55041.
KOiK07846.
OMAiEDPCRRS.
OrthoDBiEOG091G0OCN.
PhylomeDBiP55041.
TreeFamiTF314379.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLNNVTMRQ GTVGMQPQQR WSIPADARHL MVQKDPHPCN LRNRHSTAPE
60 70 80 90 100
EHCRRSWSSD STDSVISSES GNTYYRVVLI GEQGVGKSTL ANIFAGVHDS
110 120 130 140 150
MDSDCEVLGE DTYERTLVVD GESATIILLD MWENKGENEW LHDHCMQVGD
160 170 180 190 200
AYLIVYSITD RASFEKASEL RIQLRRARQT EDIPIILVGN KSDLVRCREV
210 220 230 240 250
SVSEGRACAV VFDCKFIETS AAVQHNVKEL FEGIVRQVRL RRDSKEKNER
260 270 280 290
RLAYQKRRES IPRKARRFWG KIVAKNNKNM AFKLKSKSCH DLSVL
Length:295
Mass (Da):33,725
Last modified:November 7, 2003 - v2
Checksum:i4618F225D4FAAFA5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23I → M in AAA64912 (PubMed:7912851).Curated1
Sequence conflicti56S → T in AAA64912 (PubMed:7912851).Curated1
Sequence conflicti235V → E in AAA64912 (PubMed:7912851).Curated1
Sequence conflicti241R → P in AAA64912 (PubMed:7912851).Curated1
Sequence conflicti282 – 284FKL → SSS in AAA64912 (PubMed:7912851).Curated3
Sequence conflicti294V → W in AAC52145 (PubMed:7809057).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10551 mRNA. Translation: AAA64912.1.
U13053 mRNA. Translation: AAC52145.1.
BC029668 mRNA. Translation: AAH29668.1.
CCDSiCCDS17971.1.
PIRiB54575.
I49117.
RefSeqiNP_034406.2. NM_010276.4.
UniGeneiMm.247486.

Genome annotation databases

EnsembliENSMUST00000029868; ENSMUSP00000029868; ENSMUSG00000028214.
ENSMUST00000108304; ENSMUSP00000103939; ENSMUSG00000028214.
GeneIDi14579.
KEGGimmu:14579.
UCSCiuc008rzv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10551 mRNA. Translation: AAA64912.1.
U13053 mRNA. Translation: AAC52145.1.
BC029668 mRNA. Translation: AAH29668.1.
CCDSiCCDS17971.1.
PIRiB54575.
I49117.
RefSeqiNP_034406.2. NM_010276.4.
UniGeneiMm.247486.

3D structure databases

ProteinModelPortaliP55041.
SMRiP55041.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP55041. 2 interactors.
STRINGi10090.ENSMUSP00000029868.

PTM databases

iPTMnetiP55041.
PhosphoSitePlusiP55041.

Proteomic databases

EPDiP55041.
PaxDbiP55041.
PeptideAtlasiP55041.
PRIDEiP55041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029868; ENSMUSP00000029868; ENSMUSG00000028214.
ENSMUST00000108304; ENSMUSP00000103939; ENSMUSG00000028214.
GeneIDi14579.
KEGGimmu:14579.
UCSCiuc008rzv.2. mouse.

Organism-specific databases

CTDi2669.
MGIiMGI:99844. Gem.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133672.
HOGENOMiHOG000246961.
HOVERGENiHBG104899.
InParanoidiP55041.
KOiK07846.
OMAiEDPCRRS.
OrthoDBiEOG091G0OCN.
PhylomeDBiP55041.
TreeFamiTF314379.

Miscellaneous databases

PROiP55041.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028214.
ExpressionAtlasiP55041. baseline and differential.
GenevisibleiP55041. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGEM_MOUSE
AccessioniPrimary (citable) accession number: P55041
Secondary accession number(s): Q8JZS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 7, 2003
Last modified: November 30, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.