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P55041 (GEM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP-binding protein GEM
Alternative name(s):
GTP-binding mitogen-induced T-cell protein
RAS-like protein KIR
Gene names
Name:Gem
Synonyms:Kir
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length295 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity.

Subunit structure

Interacts with calmodulin in a Ca2+-dependent manner. Calmodulin binding significantly decreases GTP binding. Binds ROCK1 By similarity. Ref.4

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Induction

By mitogens.

Post-translational modification

Phosphorylated on tyrosine residues.

Sequence similarities

Belongs to the small GTPase superfamily. RGK family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

YWHABP319463EBI-7082069,EBI-359815From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 295295GTP-binding protein GEM
PRO_0000122476

Regions

Nucleotide binding81 – 888GTP By similarity
Nucleotide binding190 – 1934GTP By similarity
Region265 – 28420Calmodulin-binding

Experimental info

Mutagenesis2691W → G: No calmodulin binding. Ref.4
Sequence conflict231I → M in AAA64912. Ref.1
Sequence conflict561S → T in AAA64912. Ref.1
Sequence conflict2351V → E in AAA64912. Ref.1
Sequence conflict2411R → P in AAA64912. Ref.1
Sequence conflict282 – 2843FKL → SSS in AAA64912. Ref.1
Sequence conflict2941V → W in AAC52145. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P55041 [UniParc].

Last modified November 7, 2003. Version 2.
Checksum: 4618F225D4FAAFA5

FASTA29533,725
        10         20         30         40         50         60 
MTLNNVTMRQ GTVGMQPQQR WSIPADARHL MVQKDPHPCN LRNRHSTAPE EHCRRSWSSD 

        70         80         90        100        110        120 
STDSVISSES GNTYYRVVLI GEQGVGKSTL ANIFAGVHDS MDSDCEVLGE DTYERTLVVD 

       130        140        150        160        170        180 
GESATIILLD MWENKGENEW LHDHCMQVGD AYLIVYSITD RASFEKASEL RIQLRRARQT 

       190        200        210        220        230        240 
EDIPIILVGN KSDLVRCREV SVSEGRACAV VFDCKFIETS AAVQHNVKEL FEGIVRQVRL 

       250        260        270        280        290 
RRDSKEKNER RLAYQKRRES IPRKARRFWG KIVAKNNKNM AFKLKSKSCH DLSVL 

« Hide

References

« Hide 'large scale' references
[1]"Gem: an induced, immediate early protein belonging to the Ras family."
Maguire J., Santoro T., Jensen P., Siebenlist U., Yewdell J., Kelly K.
Science 265:241-244(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Transcriptional activation of a ras-like gene (kir) by oncogenic tyrosine kinases."
Cohen L., Mohr R., Chen Y.-Y., Huang M., Kato R., Dorin D., Tamanoi F., Goga A., Afar D., Rosenberg N., Witte O.
Proc. Natl. Acad. Sci. U.S.A. 91:12448-12452(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Retina.
[4]"Calmodulin binds to and inhibits GTP binding of the ras-like GTPase Kir/Gem."
Fischer R., Wei Y., Anagli J., Berchtold M.W.
J. Biol. Chem. 271:25067-25070(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CALMODULIN, MUTAGENESIS OF TRP-269.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10551 mRNA. Translation: AAA64912.1.
U13053 mRNA. Translation: AAC52145.1.
BC029668 mRNA. Translation: AAH29668.1.
CCDSCCDS17971.1.
PIRB54575.
I49117.
RefSeqNP_034406.2. NM_010276.4.
UniGeneMm.247486.

3D structure databases

ProteinModelPortalP55041.
SMRP55041. Positions 72-243.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP55041. 2 interactions.

PTM databases

PhosphoSiteP55041.

Proteomic databases

PRIDEP55041.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029868; ENSMUSP00000029868; ENSMUSG00000028214.
ENSMUST00000108304; ENSMUSP00000103939; ENSMUSG00000028214.
GeneID14579.
KEGGmmu:14579.
UCSCuc008rzv.2. mouse.

Organism-specific databases

CTD2669.
MGIMGI:99844. Gem.

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000246961.
HOVERGENHBG104899.
InParanoidP55041.
KOK07846.
OMAAPEDHCR.
OrthoDBEOG7Q8CP8.
PhylomeDBP55041.
TreeFamTF314379.

Gene expression databases

ArrayExpressP55041.
BgeeP55041.
GenevestigatorP55041.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERPTHR24070. PTHR24070. 1 hit.
PfamPF00071. Ras. 1 hit.
[Graphical view]
PIRSFPIRSF038017. GTP-binding_GEM. 1 hit.
PRINTSPR00449. RASTRNSFRMNG.
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio286302.
PROP55041.
SOURCESearch...

Entry information

Entry nameGEM_MOUSE
AccessionPrimary (citable) accession number: P55041
Secondary accession number(s): Q8JZS1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 7, 2003
Last modified: July 9, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot