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P55040

- GEM_HUMAN

UniProt

P55040 - GEM_HUMAN

Protein

GTP-binding protein GEM

Gene

GEM

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi82 – 898GTPBy similarity
    Nucleotide bindingi191 – 1944GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: BHF-UCL
    2. GTPase activity Source: BHF-UCL
    3. GTP binding Source: BHF-UCL
    4. magnesium ion binding Source: BHF-UCL
    5. protein binding Source: IntAct

    GO - Biological processi

    1. cell surface receptor signaling pathway Source: ProtInc
    2. chromosome organization Source: UniProtKB
    3. immune response Source: ProtInc
    4. metaphase plate congression Source: UniProt
    5. mitotic nuclear division Source: UniProtKB
    6. signal transduction Source: ProtInc
    7. small GTPase mediated signal transduction Source: InterPro

    Keywords - Ligandi

    Calmodulin-binding, GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP-binding protein GEM
    Alternative name(s):
    GTP-binding mitogen-induced T-cell protein
    RAS-like protein KIR
    Gene namesi
    Name:GEM
    Synonyms:KIR
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:4234. GEM.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic side of plasma membrane Source: BHF-UCL
    2. midbody Source: UniProtKB
    3. mitotic spindle Source: UniProtKB
    4. nucleus Source: Ensembl
    5. spindle midzone Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28645.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 296296GTP-binding protein GEMPRO_0000122475Add
    BLAST

    Post-translational modificationi

    Phosphorylated on tyrosine residues.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP55040.
    PaxDbiP55040.
    PRIDEiP55040.

    PTM databases

    PhosphoSiteiP55040.

    Expressioni

    Tissue specificityi

    Most abundant in thymus, spleen, kidney, lung, and testis. Less abundant in heart, brain, liver and skeletal muscle.

    Inductioni

    By mitogens.

    Gene expression databases

    ArrayExpressiP55040.
    BgeeiP55040.
    CleanExiHS_GEM.
    GenevestigatoriP55040.

    Organism-specific databases

    HPAiHPA024798.

    Interactioni

    Subunit structurei

    Interacts with calmodulin in a Ca2+-dependent manner. Binds ROCK1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TRIM23P364063EBI-744104,EBI-740098
    YWHABP319463EBI-744104,EBI-359815

    Protein-protein interaction databases

    BioGridi108937. 15 interactions.
    DIPiDIP-41587N.
    IntActiP55040. 15 interactions.
    MINTiMINT-1468979.
    STRINGi9606.ENSP00000297596.

    Structurei

    Secondary structure

    1
    296
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi75 – 817
    Helixi88 – 9811
    Helixi107 – 1093
    Beta strandi113 – 1208
    Beta strandi123 – 1308
    Turni133 – 1364
    Helixi143 – 1453
    Helixi146 – 1494
    Beta strandi151 – 1588
    Helixi162 – 17817
    Turni179 – 1813
    Beta strandi186 – 1916
    Helixi196 – 1983
    Helixi203 – 21210
    Beta strandi216 – 2194
    Turni222 – 2254
    Helixi228 – 24316
    Helixi246 – 25712

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CJWX-ray2.10A/B74-261[»]
    2G3YX-ray2.40A62-249[»]
    2HT6X-ray2.40A/B71-243[»]
    ProteinModelPortaliP55040.
    SMRiP55040. Positions 73-244.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP55040.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni266 – 28520Calmodulin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. RGK family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000246961.
    HOVERGENiHBG104899.
    InParanoidiP55040.
    KOiK07846.
    OMAiAPEDHCR.
    OrthoDBiEOG7Q8CP8.
    PhylomeDBiP55040.
    TreeFamiTF314379.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR017358. Small_GTPase_GEM/REM/Rad.
    IPR020849. Small_GTPase_Ras.
    [Graphical view]
    PANTHERiPTHR24070. PTHR24070. 1 hit.
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
    PRINTSiPR00449. RASTRNSFRMNG.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51421. RAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P55040-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLNNVTMRQ GTVGMQPQQQ RWSIPADGRH LMVQKEPHQY SHRNRHSATP    50
    EDHCRRSWSS DSTDSVISSE SGNTYYRVVL IGEQGVGKST LANIFAGVHD 100
    SMDSDCEVLG EDTYERTLMV DGESATIILL DMWENKGENE WLHDHCMQVG 150
    DAYLIVYSIT DRASFEKASE LRIQLRRARQ TEDIPIILVG NKSDLVRCRE 200
    VSVSEGRACA VVFDCKFIET SAAVQHNVKE LFEGIVRQVR LRRDSKEKNE 250
    RRLAYQKRKE SMPRKARRFW GKIVAKNNKN MAFKLKSKSC HDLSVL 296
    Length:296
    Mass (Da):33,949
    Last modified:October 1, 1996 - v1
    Checksum:iB7BB3F2CA96D338F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti159 – 1591I → V in AAC50067. (PubMed:7809057)Curated
    Sequence conflicti174 – 1752QL → HV in AAC50067. (PubMed:7809057)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti43 – 431R → G.
    Corresponds to variant rs2170363 [ dbSNP | Ensembl ].
    VAR_020097

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10550 mRNA. Translation: AAA64911.1.
    U13052 mRNA. Translation: AAC50067.1.
    AK314017 mRNA. Translation: BAG36728.1.
    CH471060 Genomic DNA. Translation: EAW91709.1.
    BC022010 mRNA. Translation: AAH22010.1.
    CCDSiCCDS6261.1.
    PIRiA54575.
    I38745.
    RefSeqiNP_005252.1. NM_005261.3.
    NP_859053.1. NM_181702.2.
    UniGeneiHs.654463.

    Genome annotation databases

    EnsembliENST00000297596; ENSP00000297596; ENSG00000164949.
    ENST00000396194; ENSP00000379497; ENSG00000164949.
    GeneIDi2669.
    KEGGihsa:2669.
    UCSCiuc003ygi.3. human.

    Polymorphism databases

    DMDMi1707896.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10550 mRNA. Translation: AAA64911.1 .
    U13052 mRNA. Translation: AAC50067.1 .
    AK314017 mRNA. Translation: BAG36728.1 .
    CH471060 Genomic DNA. Translation: EAW91709.1 .
    BC022010 mRNA. Translation: AAH22010.1 .
    CCDSi CCDS6261.1.
    PIRi A54575.
    I38745.
    RefSeqi NP_005252.1. NM_005261.3.
    NP_859053.1. NM_181702.2.
    UniGenei Hs.654463.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CJW X-ray 2.10 A/B 74-261 [» ]
    2G3Y X-ray 2.40 A 62-249 [» ]
    2HT6 X-ray 2.40 A/B 71-243 [» ]
    ProteinModelPortali P55040.
    SMRi P55040. Positions 73-244.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108937. 15 interactions.
    DIPi DIP-41587N.
    IntActi P55040. 15 interactions.
    MINTi MINT-1468979.
    STRINGi 9606.ENSP00000297596.

    PTM databases

    PhosphoSitei P55040.

    Polymorphism databases

    DMDMi 1707896.

    Proteomic databases

    MaxQBi P55040.
    PaxDbi P55040.
    PRIDEi P55040.

    Protocols and materials databases

    DNASUi 2669.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000297596 ; ENSP00000297596 ; ENSG00000164949 .
    ENST00000396194 ; ENSP00000379497 ; ENSG00000164949 .
    GeneIDi 2669.
    KEGGi hsa:2669.
    UCSCi uc003ygi.3. human.

    Organism-specific databases

    CTDi 2669.
    GeneCardsi GC08M095330.
    HGNCi HGNC:4234. GEM.
    HPAi HPA024798.
    MIMi 600164. gene.
    neXtProti NX_P55040.
    PharmGKBi PA28645.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000246961.
    HOVERGENi HBG104899.
    InParanoidi P55040.
    KOi K07846.
    OMAi APEDHCR.
    OrthoDBi EOG7Q8CP8.
    PhylomeDBi P55040.
    TreeFami TF314379.

    Miscellaneous databases

    EvolutionaryTracei P55040.
    GeneWikii GEM_(gene).
    GenomeRNAii 2669.
    NextBioi 10532.
    PROi P55040.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P55040.
    Bgeei P55040.
    CleanExi HS_GEM.
    Genevestigatori P55040.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR017358. Small_GTPase_GEM/REM/Rad.
    IPR020849. Small_GTPase_Ras.
    [Graphical view ]
    PANTHERi PTHR24070. PTHR24070. 1 hit.
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038017. GTP-binding_GEM. 1 hit.
    PRINTSi PR00449. RASTRNSFRMNG.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51421. RAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gem: an induced, immediate early protein belonging to the Ras family."
      Maguire J., Santoro T., Jensen P., Siebenlist U., Yewdell J., Kelly K.
      Science 265:241-244(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Peripheral blood.
    2. "Transcriptional activation of a ras-like gene (kir) by oncogenic tyrosine kinases."
      Cohen L., Mohr R., Chen Y.-Y., Huang M., Kato R., Dorin D., Tamanoi F., Goga A., Afar D., Rosenberg N., Witte O.
      Proc. Natl. Acad. Sci. U.S.A. 91:12448-12452(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Amygdala.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    6. "Rad and Rad-related GTPases interact with calmodulin and calmodulin-dependent protein kinase II."
      Moyers J.S., Bilan P.J., Zhu J., Kahn C.R.
      J. Biol. Chem. 272:11832-11839(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CALMODULIN.
    7. "The GTP binding proteins Gem and Rad are negative regulators of the Rho-Rho kinase pathway."
      Ward Y., Yap S.-F., Ravichandran V., Matsumura F., Ito M., Spinelli B., Kelly K.
      J. Cell Biol. 157:291-302(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ROCK1.

    Entry informationi

    Entry nameiGEM_HUMAN
    AccessioniPrimary (citable) accession number: P55040
    Secondary accession number(s): B2RA31
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 147 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3