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Protein

GTP-binding protein GEM

Gene

GEM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 898GTPBy similarity
Nucleotide bindingi191 – 1944GTPBy similarity

GO - Molecular functioni

  • GDP binding Source: BHF-UCL
  • GTPase activity Source: BHF-UCL
  • GTP binding Source: BHF-UCL
  • magnesium ion binding Source: BHF-UCL

GO - Biological processi

  • cell surface receptor signaling pathway Source: ProtInc
  • chromosome organization Source: UniProtKB
  • immune response Source: ProtInc
  • metabolic process Source: GOC
  • metaphase plate congression Source: UniProtKB
  • mitotic nuclear division Source: UniProtKB
  • signal transduction Source: ProtInc
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding, GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein GEM
Alternative name(s):
GTP-binding mitogen-induced T-cell protein
RAS-like protein KIR
Gene namesi
Name:GEM
Synonyms:KIR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:4234. GEM.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic side of plasma membrane Source: BHF-UCL
  • midbody Source: UniProtKB
  • mitotic spindle Source: UniProtKB
  • nucleus Source: Ensembl
  • spindle midzone Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28645.

Polymorphism and mutation databases

BioMutaiGEM.
DMDMi1707896.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296GTP-binding protein GEMPRO_0000122475Add
BLAST

Post-translational modificationi

Phosphorylated on tyrosine residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP55040.
PaxDbiP55040.
PRIDEiP55040.

PTM databases

PhosphoSiteiP55040.

Expressioni

Tissue specificityi

Most abundant in thymus, spleen, kidney, lung, and testis. Less abundant in heart, brain, liver and skeletal muscle.

Inductioni

By mitogens.

Gene expression databases

BgeeiP55040.
CleanExiHS_GEM.
ExpressionAtlasiP55040. baseline and differential.
GenevestigatoriP55040.

Organism-specific databases

HPAiHPA024798.

Interactioni

Subunit structurei

Interacts with calmodulin in a Ca2+-dependent manner. Binds ROCK1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AHCYL1O438655EBI-744104,EBI-2371423
BLZF1Q9H2G93EBI-744104,EBI-2548012
CCDC102BA1A4H13EBI-744104,EBI-10171570
CCDC67E9PJR53EBI-744104,EBI-10177066
CCDC67Q05D603EBI-744104,EBI-748597
COG8Q96MW53EBI-744104,EBI-720875
GOLGA2Q083793EBI-744104,EBI-618309
IKZF3Q9UKT93EBI-744104,EBI-747204
INO80EQ8NBZ03EBI-744104,EBI-769401
KCTD9Q7L2733EBI-744104,EBI-4397613
KRT31Q153233EBI-744104,EBI-948001
KRT40Q6A1623EBI-744104,EBI-10171697
KRTAP10-7P604093EBI-744104,EBI-10172290
LZTS2Q9BRK43EBI-744104,EBI-741037
NOTCH2NLQ7Z3S93EBI-744104,EBI-945833
PNMA1Q8ND903EBI-744104,EBI-302345
RUNDC3AQ59EK93EBI-744104,EBI-747225
SSX2IPQ9Y2D83EBI-744104,EBI-2212028
TCF4P158843EBI-744104,EBI-533224
TFIP11Q9UBB93EBI-744104,EBI-1105213
TRAF1Q130773EBI-744104,EBI-359224
TRIM23P364063EBI-744104,EBI-740098
TRIM27P143733EBI-744104,EBI-719493
TRIM54Q9BYV23EBI-744104,EBI-2130429
YWHABP319463EBI-744104,EBI-359815

Protein-protein interaction databases

BioGridi108937. 39 interactions.
DIPiDIP-41587N.
IntActiP55040. 38 interactions.
MINTiMINT-1468979.
STRINGi9606.ENSP00000297596.

Structurei

Secondary structure

1
296
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi75 – 817Combined sources
Helixi88 – 9811Combined sources
Helixi107 – 1093Combined sources
Beta strandi113 – 1208Combined sources
Beta strandi123 – 1308Combined sources
Turni133 – 1364Combined sources
Helixi143 – 1453Combined sources
Helixi146 – 1494Combined sources
Beta strandi151 – 1588Combined sources
Helixi162 – 17817Combined sources
Turni179 – 1813Combined sources
Beta strandi186 – 1916Combined sources
Helixi196 – 1983Combined sources
Helixi203 – 21210Combined sources
Beta strandi216 – 2194Combined sources
Turni222 – 2254Combined sources
Helixi228 – 24316Combined sources
Helixi246 – 25712Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CJWX-ray2.10A/B74-261[»]
2G3YX-ray2.40A62-249[»]
2HT6X-ray2.40A/B71-243[»]
ProteinModelPortaliP55040.
SMRiP55040. Positions 73-244.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55040.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni266 – 28520Calmodulin-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the small GTPase superfamily. RGK family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121849.
HOGENOMiHOG000246961.
HOVERGENiHBG104899.
InParanoidiP55040.
KOiK07846.
OMAiAPEDHCR.
OrthoDBiEOG7Q8CP8.
PhylomeDBiP55040.
TreeFamiTF314379.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
PRINTSiPR00449. RASTRNSFRMNG.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLNNVTMRQ GTVGMQPQQQ RWSIPADGRH LMVQKEPHQY SHRNRHSATP
60 70 80 90 100
EDHCRRSWSS DSTDSVISSE SGNTYYRVVL IGEQGVGKST LANIFAGVHD
110 120 130 140 150
SMDSDCEVLG EDTYERTLMV DGESATIILL DMWENKGENE WLHDHCMQVG
160 170 180 190 200
DAYLIVYSIT DRASFEKASE LRIQLRRARQ TEDIPIILVG NKSDLVRCRE
210 220 230 240 250
VSVSEGRACA VVFDCKFIET SAAVQHNVKE LFEGIVRQVR LRRDSKEKNE
260 270 280 290
RRLAYQKRKE SMPRKARRFW GKIVAKNNKN MAFKLKSKSC HDLSVL
Length:296
Mass (Da):33,949
Last modified:October 1, 1996 - v1
Checksum:iB7BB3F2CA96D338F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti159 – 1591I → V in AAC50067 (PubMed:7809057).Curated
Sequence conflicti174 – 1752QL → HV in AAC50067 (PubMed:7809057).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti43 – 431R → G.
Corresponds to variant rs2170363 [ dbSNP | Ensembl ].
VAR_020097

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10550 mRNA. Translation: AAA64911.1.
U13052 mRNA. Translation: AAC50067.1.
AK314017 mRNA. Translation: BAG36728.1.
CH471060 Genomic DNA. Translation: EAW91709.1.
BC022010 mRNA. Translation: AAH22010.1.
CCDSiCCDS6261.1.
PIRiA54575.
I38745.
RefSeqiNP_005252.1. NM_005261.3.
NP_859053.1. NM_181702.2.
UniGeneiHs.654463.

Genome annotation databases

EnsembliENST00000297596; ENSP00000297596; ENSG00000164949.
ENST00000396194; ENSP00000379497; ENSG00000164949.
GeneIDi2669.
KEGGihsa:2669.
UCSCiuc003ygi.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10550 mRNA. Translation: AAA64911.1.
U13052 mRNA. Translation: AAC50067.1.
AK314017 mRNA. Translation: BAG36728.1.
CH471060 Genomic DNA. Translation: EAW91709.1.
BC022010 mRNA. Translation: AAH22010.1.
CCDSiCCDS6261.1.
PIRiA54575.
I38745.
RefSeqiNP_005252.1. NM_005261.3.
NP_859053.1. NM_181702.2.
UniGeneiHs.654463.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CJWX-ray2.10A/B74-261[»]
2G3YX-ray2.40A62-249[»]
2HT6X-ray2.40A/B71-243[»]
ProteinModelPortaliP55040.
SMRiP55040. Positions 73-244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108937. 39 interactions.
DIPiDIP-41587N.
IntActiP55040. 38 interactions.
MINTiMINT-1468979.
STRINGi9606.ENSP00000297596.

PTM databases

PhosphoSiteiP55040.

Polymorphism and mutation databases

BioMutaiGEM.
DMDMi1707896.

Proteomic databases

MaxQBiP55040.
PaxDbiP55040.
PRIDEiP55040.

Protocols and materials databases

DNASUi2669.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000297596; ENSP00000297596; ENSG00000164949.
ENST00000396194; ENSP00000379497; ENSG00000164949.
GeneIDi2669.
KEGGihsa:2669.
UCSCiuc003ygi.3. human.

Organism-specific databases

CTDi2669.
GeneCardsiGC08M095330.
HGNCiHGNC:4234. GEM.
HPAiHPA024798.
MIMi600164. gene.
neXtProtiNX_P55040.
PharmGKBiPA28645.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121849.
HOGENOMiHOG000246961.
HOVERGENiHBG104899.
InParanoidiP55040.
KOiK07846.
OMAiAPEDHCR.
OrthoDBiEOG7Q8CP8.
PhylomeDBiP55040.
TreeFamiTF314379.

Miscellaneous databases

EvolutionaryTraceiP55040.
GeneWikiiGEM_(gene).
GenomeRNAii2669.
NextBioi10532.
PROiP55040.
SOURCEiSearch...

Gene expression databases

BgeeiP55040.
CleanExiHS_GEM.
ExpressionAtlasiP55040. baseline and differential.
GenevestigatoriP55040.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
PRINTSiPR00449. RASTRNSFRMNG.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gem: an induced, immediate early protein belonging to the Ras family."
    Maguire J., Santoro T., Jensen P., Siebenlist U., Yewdell J., Kelly K.
    Science 265:241-244(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Peripheral blood.
  2. "Transcriptional activation of a ras-like gene (kir) by oncogenic tyrosine kinases."
    Cohen L., Mohr R., Chen Y.-Y., Huang M., Kato R., Dorin D., Tamanoi F., Goga A., Afar D., Rosenberg N., Witte O.
    Proc. Natl. Acad. Sci. U.S.A. 91:12448-12452(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Amygdala.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  6. "Rad and Rad-related GTPases interact with calmodulin and calmodulin-dependent protein kinase II."
    Moyers J.S., Bilan P.J., Zhu J., Kahn C.R.
    J. Biol. Chem. 272:11832-11839(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CALMODULIN.
  7. "The GTP binding proteins Gem and Rad are negative regulators of the Rho-Rho kinase pathway."
    Ward Y., Yap S.-F., Ravichandran V., Matsumura F., Ito M., Spinelli B., Kelly K.
    J. Cell Biol. 157:291-302(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ROCK1.

Entry informationi

Entry nameiGEM_HUMAN
AccessioniPrimary (citable) accession number: P55040
Secondary accession number(s): B2RA31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 29, 2015
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.