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P55038 (GLTS_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ferredoxin-dependent glutamate synthase 2
EC=1.4.7.1
Alternative name(s):
FD-GOGAT
Gene names
Name:gltS
Ordered Locus Names:sll1499
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis ›

Protein attributes

Sequence length1556 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+.

Cofactor

Binds 1 3Fe-4S cluster.

FAD.

FMN.

Pathway

Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1.

Energy metabolism; nitrogen metabolism.

Sequence similarities

Belongs to the glutamate synthase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15561556Ferredoxin-dependent glutamate synthase 2
PRO_0000170794

Regions

Domain37 – 431395Glutamine amidotransferase type-2

Sites

Active site371For GATase activity By similarity
Metal binding11731Iron-sulfur (3Fe-4S) By similarity
Metal binding11791Iron-sulfur (3Fe-4S) By similarity
Metal binding11841Iron-sulfur (3Fe-4S) By similarity

Experimental info

Sequence conflict4911E → Q in CAA63218. Ref.1
Sequence conflict570 – 5723ESA → NPR in CAA63218. Ref.1
Sequence conflict642 – 6509GAILTENQS → RRNIGLRIKV in CAA63218. Ref.1
Sequence conflict6591G → E in CAA63218. Ref.1
Sequence conflict940 – 9412GG → PP in CAA63218. Ref.1
Sequence conflict10591H → L in CAA63218. Ref.1
Sequence conflict12951E → RK in CAA63218. Ref.1
Sequence conflict13101V → D in CAA63218. Ref.1
Sequence conflict13231A → S in CAA63218. Ref.1
Sequence conflict15311Missing in CAA63218. Ref.1

Secondary structure

1 ......................................................................................................................................................................................................................................................................................... 1556
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P55038 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 4BDAD5F9A4064D9D

FASTA1,556169,499
        10         20         30         40         50         60 
MSFQYPLLAP MTNSSVATNS NQPFLGQPWL VEERDACGVG FIANLRGKPD HTLVEQALKA 

        70         80         90        100        110        120 
LGCMEHRGGC SADNDSGDGA GVMTAIPREL LAQWFNTRNL PMPDGDRLGV GMVFLPQEPS 

       130        140        150        160        170        180 
AREVARAYVE EVVRLEKLTV LGWREVPVNS DVLGIQAKNN QPHIEQILVT CPEGCAGDEL 

       190        200        210        220        230        240 
DRRLYIARSI IGKKLAEDFY VCSFSCRTIV YKGMVRSIIL GEFYLDLKNP GYTSNFAVYH 

       250        260        270        280        290        300 
RRFSTNTMPK WPLAQPMRLL GHNGEINTLL GNINWMAARE KELEVSGWTK AELEALTPIV 

       310        320        330        340        350        360 
NQANSDSYNL DSALELLVRT GRSPLEAAMI LVPEAYKNQP ALKDYPEISD FHDYYSGLQE 

       370        380        390        400        410        420 
PWDGPALLVF SDGKIVGAGL DRNGLRPARY CITKDDYIVL GSEAGVVDLP EVDIVEKGRL 

       430        440        450        460        470        480 
APGQMIAVDL AEQKILKNYQ IKQQAAQKYP YGEWIKIQRQ TVASDSFAEK TLFNDAQTVL 

       490        500        510        520        530        540 
QQQAAFGYTA EDVEMVVVPM ASQGKEPTFC MGDDTPLAVL SHKPRLLYDY FKQRFAQVTN 

       550        560        570        580        590        600 
PPIDPLRENL VMSLAMFLGK RGNLLEPKAE SARTIKLRSP LVNEVELQAI KTGQLQVAEV 

       610        620        630        640        650        660 
STLYDLDGVN SLETALDNLV KTAIATVQAG AEILVLTDRP NGAILTENQS FIPPLLAVGA 

       670        680        690        700        710        720 
VHHHLIRAGL RLKASLIVDT AQCWSTHHFA CLVGYGASAI CPYLALESVR QWWLDEKTQK 

       730        740        750        760        770        780 
LMENGRLDRI DLPTALKNYR QSVEAGLFKI LSKMGISLLA SYHGAQIFEA IGLGAELVEY 

       790        800        810        820        830        840 
AFAGTTSRVG GLTIADVAGE VMVFHGMAFP EMAKKLENFG FVNYRPGGEY HMNSPEMSKS 

       850        860        870        880        890        900 
LHKAVAAYKV GGNGNNGEAY DHYELYRQYL KDRPVTALRD LLDFNADQPA ISLEEVESVE 

       910        920        930        940        950        960 
SIVKRFCTGG MSLGALSREA HETLAIAMNR LGAKSNSGEG GEDVVRYLTL DDVDSEGNSP 

       970        980        990       1000       1010       1020 
TLPHLHGLQN GDTANSAIKQ IASGRFGVTP EYLMSGKQLE IKMAQGAKPG EGGQLPGKKV 

      1030       1040       1050       1060       1070       1080 
SEYIAMLRRS KPGVTLISPP PHHDIYSIED LAQLIYDLHQ INPEAQVSVK LVAEIGIGTI 

      1090       1100       1110       1120       1130       1140 
AAGVAKANAD IIQISGHDGG TGASPLSSIK HAGSPWELGV TEVHRVLMEN QLRDRVLLRA 

      1150       1160       1170       1180       1190       1200 
DGGLKTGWDV VMAALMGAEE YGFGSIAMIA EGCIMARVCH TNNCPVGVAT QQERLRQRFK 

      1210       1220       1230       1240       1250       1260 
GVPGQVVNFF YFIAEEVRSL LAHLGYRSLD DIIGRTDLLK VRSDVQLSKT QNLTLDCLLN 

      1270       1280       1290       1300       1310       1320 
LPDTKQNRQW LNHEPVHSNG PVLDDDILAD PDIQEAINHQ TTATKTYRLV NTDRTVGTRL 

      1330       1340       1350       1360       1370       1380 
SGAIAKKYGN NGFEGNITLN FQGAAGQSFG AFNLDGMTLH LQGEANDYVG KGMNGGEIVI 

      1390       1400       1410       1420       1430       1440 
VPHPQASFAP EDNVIIGNTC LYGATGGNLY ANGRAGERFA VRNSVGKAVI EGAGDHCCEY 

      1450       1460       1470       1480       1490       1500 
MTGGVIVVLG PVGRNVGAGM TGGLAYFLDE VGDLPEKINP EIITLQRITA SKGEEQLKSL 

      1510       1520       1530       1540       1550 
ITAHVEHTGS PKGKAILANW SDYLGKFWQA VPPSEKDSPE ANGDVSLTGE KTLTSV

« Hide

References

« Hide 'large scale' references
[1]"Existence of two ferredoxin-glutamate synthases in the cyanobacterium Synechocystis sp. PCC 6803. Isolation and insertional inactivation of gltB and gltS genes."
Navarro F., Chavez S., Candau P., Florencio F.J.
Plant Mol. Biol. 27:753-767(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Terauchi K., Ikeuchi M., Ohmori M.
Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X92480 Genomic DNA. Translation: CAA63218.1.
D78371 Genomic DNA. Translation: BAA11379.1.
BA000022 Genomic DNA. Translation: BAA18693.1.
PIRS76781.
RefSeqNP_442881.1. NC_000911.1.
YP_005652942.1. NC_017277.1.
YP_007452757.1. NC_020286.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1LLWX-ray2.70A37-1556[»]
1LLZX-ray3.00A37-1556[»]
1LM1X-ray2.80A37-1556[»]
1OFDX-ray2.00A/B37-1556[»]
1OFEX-ray2.45A/B37-1556[»]
ProteinModelPortalP55038.
ModBaseSearch...

Protein-protein interaction databases

IntActP55038. 4 interactions.
STRING1148.sll1499.

Proteomic databases

PaxDbP55038.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA18693; BAA18693; BAA18693.
GeneID12255312.
14618447.
952019.
KEGGsyn:sll1499.
syy:SYNGTS_2989.
PATRIC23843556. VBISynSp132158_3309.

Phylogenomic databases

eggNOGCOG0069.
HOGENOMHOG000031558.
KOK00284.
OMARMSASIV.
ProtClustDBCLSK893761.

Enzyme and pathway databases

UniPathwayUPA00045.
UPA00634; UER00691.

Family and domain databases

Gene3D2.160.20.60. 1 hit.
3.20.20.70. 2 hits.
InterProIPR013785. Aldolase_TIM.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR002489. Glu_synth_asu_C.
IPR002932. Glu_synth_centr_C.
IPR006982. Glu_synth_centr_N.
[Graphical view]
PfamPF00310. GATase_2. 1 hit.
PF04898. Glu_syn_central. 1 hit.
PF01645. Glu_synthase. 1 hit.
PF01493. GXGXG. 1 hit.
[Graphical view]
SUPFAMSSF69336. Glu_synthase_C. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP55038.

Entry information

Entry nameGLTS_SYNY3
AccessionPrimary (citable) accession number: P55038
Secondary accession number(s): Q59980
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents