Reviewed, UniProtKB/Swiss-Prot P55037 (GLTB_SYNY3)

Names and origin

Protein names

Recommended name:
    Ferredoxin-dependent glutamate synthase 1
    EC=1.4.7.1
Alternative name(s):
    Fd-GOGAT

Gene names

Name:	gltB
Ordered Locus Names:	sll1502

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	1550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+.
Cofactor	Binds 1 3Fe-4S cluster. FAD. FMN.
Pathway	Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1. Energy metabolism; nitrogen metabolism.
Sequence similarities	Belongs to the glutamate synthase family. Contains 1 glutamine amidotransferase type-2 domain.
Caution	It is uncertain whether Met-1 or Met-21 is the initiator.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Glutamate biosynthesis
Domain	Glutamine amidotransferase
Ligand	3Fe-4S FAD FMN Flavoprotein Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate synthase (ferredoxin) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
trxA	P52231	2	EBI-862093,EBI-862916

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1550	1550	Ferredoxin-dependent glutamate synthase 1		PRO_0000170793
Regions
	Domain	43 – 440	398	Glutamine amidotransferase type-2
	Nucleotide binding	1097 – 1154	58	FMN By similarity
Sites
	Active site	43	1	For GATase activity By similarity
	Metal binding	1150	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	1156	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	1161	1	Iron-sulfur (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55037-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 07AA64D832296943	FASTA	1,550	169,071
	10 20 30 40 50 60 MPCHEGLHPL VPNFCTVTSP MNSSHLAPQV QGLYDPQNEH DACGVGFIVQ MKGKVSHDIV 70 80 90 100 110 120 EQGLQMLVNL EHRGACGCEP NTGDGAGILI QVPHKFIQKI AGAEGITIPA PGQYAVGNIY 130 140 150 160 170 180 GSPDPLARAE ARQKFNDIVA QEGLKVLGWR DIPTQNEPLG ETAIASEPFM QQVYIARPEG 190 200 210 220 230 240 LTDDLDFERK LYVIRKLTHG AIRSPKIDTY WYVASLSART LVYKGMLTTA QVGQYYPELH 250 260 270 280 290 300 DPDMESALAL VHSRFSTNTF PSWERSHPYR YIAHNGEINT MRGNVNWMQA RQALFESSLF 310 320 330 340 350 360 GEDMAKVQPV INIDGSDSTI FDNALELLYL AGRSLPHAVM MMIPEPWSAH ESMSQEKKAF 370 380 390 400 410 420 YKYHSCLMEP WDGPASIAFT NGKMMGAVLD RNGLRPSRYY VTKDDLVIMA SEAGVLPIEP 430 440 450 460 470 480 ERVAKKGRLQ PGRMFLVDME QGRIIADEEI KQEIVSQHPY GEWLAANLKS LEQLPSPGNV 490 500 510 520 530 540 PGTDAESLRQ RQMAFGYTFE ELRILLAPMG RDGVEAIGSM GADTPLAVLS DKPKLLYNYF 550 560 570 580 590 600 QQLFAQVTNP PIDSIREEII TSAETTIGGE GNLLDPRPES CRLIELKTPI LTNEDLAKLK 610 620 630 640 650 660 ALDDDEFKSV TLDILFDPNQ GEAGLKTALD NLFTEADQAI SQGANLIILS DRQVSAEKAA 670 680 690 700 710 720 IPALLAVSGL HHHLIRNGSR TKVGLVLESG EPREVHHFAV LLGYGCGAIN PYLAFETLDG 730 740 750 760 770 780 MIAEGLLVNV DHKTACKNYI KAATKGVIKV ASKIGISTIQ SYRGAQIFEA VGLNQSVIDE 790 800 810 820 830 840 YFCRTSSRIQ GSDLGVIAQE AILRHQHAFA PRPGDLHTLD VGGEYQWRKD GEEHLFSPQT 850 860 870 880 890 900 IHLLQRAVRE GNYELYKQYA ALVNEQNQKF FTLRGLLDFQ DRESIPLEEV EPIEAIMKRF 910 920 930 940 950 960 KTGAMSYGSI SKEAHESLAI AMNRIGGKSN TGEGGEDPER FTWTNDQGDS KNSAIKQVAS 970 980 990 1000 1010 1020 GRFGVTSLYL SQAKEIQIKM AQGAKPGEGG QLPGKKVYPW IAKVRHSTPG VGLISPPPHH 1030 1040 1050 1060 1070 1080 DIYSIEDLAE LIHDLKNANR EARINVKLVS EVGVGTIAAG VAKAHADVVL VSGYDGGTGA 1090 1100 1110 1120 1130 1140 SPQTSIKHAG LPWELGLAET HQTLVLNNLR SRIVVETDGQ MKTGRDVAIA ALLGAEEFGF 1150 1160 1170 1180 1190 1200 STAPLVSLGC IMMRACHLNT CPVGIATQNP ELRAKFTGDP AHAVNFMTFI ATELREVMAQ 1210 1220 1230 1240 1250 1260 LGFRTINEMV GRTDILEPKK AVAHWKAKGI DLSTILHQPE VGDDVGRYCQ IPQDHGLQHS 1270 1280 1290 1300 1310 1320 LDITQLLDLC QPAIAKGEKV TATLPITNIN RVVGTIVGNE ITKRHWEGLP EDTVHLHFQG 1330 1340 1350 1360 1370 1380 SAGQSFGAFI PKGMTLELEG DANDYLGKGL SGGKIIVYPP KGSSFIASEN IIAGNVCLYG 1390 1400 1410 1420 1430 1440 ATAGEVYISG MVGERFCVRN SGVNTVVEAV GDHGCEYMTG GKVVVLGQTG RNFAAGMSGG 1450 1460 1470 1480 1490 1500 VAYIFDETGD FATRCNSAMV GLEKLEDPEE IKDLKELIQN HVNYTDSAKG KAVLADWEAS 1510 1520 1530 1540 1550 IPKFVKVMPR DYKRVLQAIK KALEAGLSGD DALNAAFEEN AKDVARIGGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Existence of two ferredoxin-glutamate synthases in the cyanobacterium Synechocystis sp. PCC 6803. Isolation and insertional inactivation of gltB and gltS genes." Navarro F., Chavez S., Candau P., Florencio F.J. Plant Mol. Biol. 27:753-767(1995) [PubMed: 7727752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X80485 Genomic DNA. Translation: CAA56652.1. BA000022 Genomic DNA. Translation: BAA17018.1.
PIR	S60228.
RefSeq	NP_440338.1.
3D structure databases
HSSP	HSSP built from PDB template 1LM1 based on UniProtKB P55038.
ModBase	Search...
Protein-protein interaction databases
IntAct	P55037. 3 interactions.
Genome annotation databases
GeneID	953637.
GenomeReviews	Gene locus sll1502 in contig BA000022_GR.
KEGG	syn:sll1502.
NMPDR	fig|1148.1.peg.439.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P55037.
OMA	P55037. WMAARQA.
Enzyme and pathway databases
BioCyc	SSP1148:SLL1502-MON.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000583. GATase_2. IPR017932. GATase_II. IPR002932. Glu_synth_centr_C. IPR006982. Glu_synth_centr_N. IPR002489. Glu_synthase_C. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 2 hits. G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF04898. Glu_syn_central. 1 hit. PF01645. Glu_synthase. 1 hit. PF01493. GXGXG. 1 hit. [Graphical view]
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLTB_SYNY3

Accession

Primary (citable) accession number: P55037

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)