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P55037 (GLTB_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ferredoxin-dependent glutamate synthase 1

EC=1.4.7.1
Alternative name(s):
Fd-GOGAT
Gene names
Name:gltB
Ordered Locus Names:sll1502
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length1550 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+.

Cofactor

Binds 1 3Fe-4S cluster.

FAD.

FMN.

Pathway

Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1.

Energy metabolism; nitrogen metabolism.

Sequence similarities

Belongs to the glutamate synthase family.

Contains 1 glutamine amidotransferase type-2 domain.

Caution

It is uncertain whether Met-1 or Met-21 is the initiator.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

trxAP522312EBI-862093,EBI-862916

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15501550Ferredoxin-dependent glutamate synthase 1
PRO_0000170793

Regions

Domain43 – 440398Glutamine amidotransferase type-2
Nucleotide binding1097 – 115458FMN By similarity

Sites

Active site431For GATase activity By similarity
Metal binding11501Iron-sulfur (3Fe-4S) By similarity
Metal binding11561Iron-sulfur (3Fe-4S) By similarity
Metal binding11611Iron-sulfur (3Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P55037 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 07AA64D832296943

FASTA1,550169,071
        10         20         30         40         50         60 
MPCHEGLHPL VPNFCTVTSP MNSSHLAPQV QGLYDPQNEH DACGVGFIVQ MKGKVSHDIV 

        70         80         90        100        110        120 
EQGLQMLVNL EHRGACGCEP NTGDGAGILI QVPHKFIQKI AGAEGITIPA PGQYAVGNIY 

       130        140        150        160        170        180 
GSPDPLARAE ARQKFNDIVA QEGLKVLGWR DIPTQNEPLG ETAIASEPFM QQVYIARPEG 

       190        200        210        220        230        240 
LTDDLDFERK LYVIRKLTHG AIRSPKIDTY WYVASLSART LVYKGMLTTA QVGQYYPELH 

       250        260        270        280        290        300 
DPDMESALAL VHSRFSTNTF PSWERSHPYR YIAHNGEINT MRGNVNWMQA RQALFESSLF 

       310        320        330        340        350        360 
GEDMAKVQPV INIDGSDSTI FDNALELLYL AGRSLPHAVM MMIPEPWSAH ESMSQEKKAF 

       370        380        390        400        410        420 
YKYHSCLMEP WDGPASIAFT NGKMMGAVLD RNGLRPSRYY VTKDDLVIMA SEAGVLPIEP 

       430        440        450        460        470        480 
ERVAKKGRLQ PGRMFLVDME QGRIIADEEI KQEIVSQHPY GEWLAANLKS LEQLPSPGNV 

       490        500        510        520        530        540 
PGTDAESLRQ RQMAFGYTFE ELRILLAPMG RDGVEAIGSM GADTPLAVLS DKPKLLYNYF 

       550        560        570        580        590        600 
QQLFAQVTNP PIDSIREEII TSAETTIGGE GNLLDPRPES CRLIELKTPI LTNEDLAKLK 

       610        620        630        640        650        660 
ALDDDEFKSV TLDILFDPNQ GEAGLKTALD NLFTEADQAI SQGANLIILS DRQVSAEKAA 

       670        680        690        700        710        720 
IPALLAVSGL HHHLIRNGSR TKVGLVLESG EPREVHHFAV LLGYGCGAIN PYLAFETLDG 

       730        740        750        760        770        780 
MIAEGLLVNV DHKTACKNYI KAATKGVIKV ASKIGISTIQ SYRGAQIFEA VGLNQSVIDE 

       790        800        810        820        830        840 
YFCRTSSRIQ GSDLGVIAQE AILRHQHAFA PRPGDLHTLD VGGEYQWRKD GEEHLFSPQT 

       850        860        870        880        890        900 
IHLLQRAVRE GNYELYKQYA ALVNEQNQKF FTLRGLLDFQ DRESIPLEEV EPIEAIMKRF 

       910        920        930        940        950        960 
KTGAMSYGSI SKEAHESLAI AMNRIGGKSN TGEGGEDPER FTWTNDQGDS KNSAIKQVAS 

       970        980        990       1000       1010       1020 
GRFGVTSLYL SQAKEIQIKM AQGAKPGEGG QLPGKKVYPW IAKVRHSTPG VGLISPPPHH 

      1030       1040       1050       1060       1070       1080 
DIYSIEDLAE LIHDLKNANR EARINVKLVS EVGVGTIAAG VAKAHADVVL VSGYDGGTGA 

      1090       1100       1110       1120       1130       1140 
SPQTSIKHAG LPWELGLAET HQTLVLNNLR SRIVVETDGQ MKTGRDVAIA ALLGAEEFGF 

      1150       1160       1170       1180       1190       1200 
STAPLVSLGC IMMRACHLNT CPVGIATQNP ELRAKFTGDP AHAVNFMTFI ATELREVMAQ 

      1210       1220       1230       1240       1250       1260 
LGFRTINEMV GRTDILEPKK AVAHWKAKGI DLSTILHQPE VGDDVGRYCQ IPQDHGLQHS 

      1270       1280       1290       1300       1310       1320 
LDITQLLDLC QPAIAKGEKV TATLPITNIN RVVGTIVGNE ITKRHWEGLP EDTVHLHFQG 

      1330       1340       1350       1360       1370       1380 
SAGQSFGAFI PKGMTLELEG DANDYLGKGL SGGKIIVYPP KGSSFIASEN IIAGNVCLYG 

      1390       1400       1410       1420       1430       1440 
ATAGEVYISG MVGERFCVRN SGVNTVVEAV GDHGCEYMTG GKVVVLGQTG RNFAAGMSGG 

      1450       1460       1470       1480       1490       1500 
VAYIFDETGD FATRCNSAMV GLEKLEDPEE IKDLKELIQN HVNYTDSAKG KAVLADWEAS 

      1510       1520       1530       1540       1550 
IPKFVKVMPR DYKRVLQAIK KALEAGLSGD DALNAAFEEN AKDVARIGGS 

« Hide

References

« Hide 'large scale' references
[1]"Existence of two ferredoxin-glutamate synthases in the cyanobacterium Synechocystis sp. PCC 6803. Isolation and insertional inactivation of gltB and gltS genes."
Navarro F., Chavez S., Candau P., Florencio F.J.
Plant Mol. Biol. 27:753-767(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X80485 Genomic DNA. Translation: CAA56652.1.
BA000022 Genomic DNA. Translation: BAA17018.1.
PIRS60228.
RefSeqNP_440338.1. NC_000911.1.
YP_005650395.1. NC_017277.1.
YP_007450221.1. NC_020286.1.

3D structure databases

ProteinModelPortalP55037.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP55037. 3 interactions.
STRING1148.sll1502.

Proteomic databases

PaxDbP55037.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA17018; BAA17018; BAA17018.
GeneID12256085.
14615869.
953637.
KEGGsyn:sll1502.
syy:SYNGTS_0442.
syz:MYO_14470.
PATRIC23837822. VBISynSp132158_0480.

Phylogenomic databases

eggNOGCOG0069.
HOGENOMHOG000031559.
KOK00284.
OMAACKNYIK.
OrthoDBEOG60W7PX.
PhylomeDBP55037.
ProtClustDBCLSK892635.

Enzyme and pathway databases

UniPathwayUPA00045.
UPA00634; UER00691.

Family and domain databases

Gene3D2.160.20.60. 1 hit.
3.20.20.70. 2 hits.
InterProIPR013785. Aldolase_TIM.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR002489. Glu_synth_asu_C.
IPR002932. Glu_synth_centr_C.
IPR006982. Glu_synth_centr_N.
[Graphical view]
PfamPF00310. GATase_2. 1 hit.
PF04898. Glu_syn_central. 1 hit.
PF01645. Glu_synthase. 1 hit.
PF01493. GXGXG. 1 hit.
[Graphical view]
SUPFAMSSF69336. SSF69336. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLTB_SYNY3
AccessionPrimary (citable) accession number: P55037
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways