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P55037

- GLTB_SYNY3

UniProt

P55037 - GLTB_SYNY3

Protein

Ferredoxin-dependent glutamate synthase 1

Gene

gltB

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+.

    Cofactori

    Binds 1 3Fe-4S cluster.
    FAD.
    FMN.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei43 – 431For GATase activityBy similarity
    Metal bindingi1150 – 11501Iron-sulfur (3Fe-4S)By similarity
    Metal bindingi1156 – 11561Iron-sulfur (3Fe-4S)By similarity
    Metal bindingi1161 – 11611Iron-sulfur (3Fe-4S)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi1097 – 115458FMNBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. glutamate synthase (ferredoxin) activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. L-glutamate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Glutamate biosynthesis

    Keywords - Ligandi

    3Fe-4S, FAD, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00045.
    UPA00634; UER00691.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ferredoxin-dependent glutamate synthase 1 (EC:1.4.7.1)
    Alternative name(s):
    Fd-GOGAT
    Gene namesi
    Name:gltB
    Ordered Locus Names:sll1502
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15501550Ferredoxin-dependent glutamate synthase 1PRO_0000170793Add
    BLAST

    Proteomic databases

    PaxDbiP55037.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    trxAP522312EBI-862093,EBI-862916

    Protein-protein interaction databases

    IntActiP55037. 3 interactions.
    STRINGi1148.sll1502.

    Structurei

    3D structure databases

    ProteinModelPortaliP55037.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 440398Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glutamate synthase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0069.
    HOGENOMiHOG000031559.
    KOiK00284.
    OMAiRRYGHAG.
    OrthoDBiEOG60W7PX.
    PhylomeDBiP55037.

    Family and domain databases

    Gene3Di2.160.20.60. 1 hit.
    3.20.20.70. 2 hits.
    3.60.20.10. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR002489. Glu_synth_asu_C.
    IPR006982. Glu_synth_centr_N.
    IPR002932. Glu_synthdom.
    IPR029055. Ntn_hydrolases_N.
    [Graphical view]
    PfamiPF00310. GATase_2. 1 hit.
    PF04898. Glu_syn_central. 1 hit.
    PF01645. Glu_synthase. 1 hit.
    PF01493. GXGXG. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    SSF69336. SSF69336. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P55037-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPCHEGLHPL VPNFCTVTSP MNSSHLAPQV QGLYDPQNEH DACGVGFIVQ     50
    MKGKVSHDIV EQGLQMLVNL EHRGACGCEP NTGDGAGILI QVPHKFIQKI 100
    AGAEGITIPA PGQYAVGNIY GSPDPLARAE ARQKFNDIVA QEGLKVLGWR 150
    DIPTQNEPLG ETAIASEPFM QQVYIARPEG LTDDLDFERK LYVIRKLTHG 200
    AIRSPKIDTY WYVASLSART LVYKGMLTTA QVGQYYPELH DPDMESALAL 250
    VHSRFSTNTF PSWERSHPYR YIAHNGEINT MRGNVNWMQA RQALFESSLF 300
    GEDMAKVQPV INIDGSDSTI FDNALELLYL AGRSLPHAVM MMIPEPWSAH 350
    ESMSQEKKAF YKYHSCLMEP WDGPASIAFT NGKMMGAVLD RNGLRPSRYY 400
    VTKDDLVIMA SEAGVLPIEP ERVAKKGRLQ PGRMFLVDME QGRIIADEEI 450
    KQEIVSQHPY GEWLAANLKS LEQLPSPGNV PGTDAESLRQ RQMAFGYTFE 500
    ELRILLAPMG RDGVEAIGSM GADTPLAVLS DKPKLLYNYF QQLFAQVTNP 550
    PIDSIREEII TSAETTIGGE GNLLDPRPES CRLIELKTPI LTNEDLAKLK 600
    ALDDDEFKSV TLDILFDPNQ GEAGLKTALD NLFTEADQAI SQGANLIILS 650
    DRQVSAEKAA IPALLAVSGL HHHLIRNGSR TKVGLVLESG EPREVHHFAV 700
    LLGYGCGAIN PYLAFETLDG MIAEGLLVNV DHKTACKNYI KAATKGVIKV 750
    ASKIGISTIQ SYRGAQIFEA VGLNQSVIDE YFCRTSSRIQ GSDLGVIAQE 800
    AILRHQHAFA PRPGDLHTLD VGGEYQWRKD GEEHLFSPQT IHLLQRAVRE 850
    GNYELYKQYA ALVNEQNQKF FTLRGLLDFQ DRESIPLEEV EPIEAIMKRF 900
    KTGAMSYGSI SKEAHESLAI AMNRIGGKSN TGEGGEDPER FTWTNDQGDS 950
    KNSAIKQVAS GRFGVTSLYL SQAKEIQIKM AQGAKPGEGG QLPGKKVYPW 1000
    IAKVRHSTPG VGLISPPPHH DIYSIEDLAE LIHDLKNANR EARINVKLVS 1050
    EVGVGTIAAG VAKAHADVVL VSGYDGGTGA SPQTSIKHAG LPWELGLAET 1100
    HQTLVLNNLR SRIVVETDGQ MKTGRDVAIA ALLGAEEFGF STAPLVSLGC 1150
    IMMRACHLNT CPVGIATQNP ELRAKFTGDP AHAVNFMTFI ATELREVMAQ 1200
    LGFRTINEMV GRTDILEPKK AVAHWKAKGI DLSTILHQPE VGDDVGRYCQ 1250
    IPQDHGLQHS LDITQLLDLC QPAIAKGEKV TATLPITNIN RVVGTIVGNE 1300
    ITKRHWEGLP EDTVHLHFQG SAGQSFGAFI PKGMTLELEG DANDYLGKGL 1350
    SGGKIIVYPP KGSSFIASEN IIAGNVCLYG ATAGEVYISG MVGERFCVRN 1400
    SGVNTVVEAV GDHGCEYMTG GKVVVLGQTG RNFAAGMSGG VAYIFDETGD 1450
    FATRCNSAMV GLEKLEDPEE IKDLKELIQN HVNYTDSAKG KAVLADWEAS 1500
    IPKFVKVMPR DYKRVLQAIK KALEAGLSGD DALNAAFEEN AKDVARIGGS 1550
    Length:1,550
    Mass (Da):169,071
    Last modified:October 1, 1996 - v1
    Checksum:i07AA64D832296943
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80485 Genomic DNA. Translation: CAA56652.1.
    BA000022 Genomic DNA. Translation: BAA17018.1.
    PIRiS60228.
    RefSeqiNP_440338.1. NC_000911.1.
    YP_005650395.1. NC_017277.1.
    YP_007450221.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA17018; BAA17018; BAA17018.
    GeneIDi953637.
    KEGGisyn:sll1502.
    syy:SYNGTS_0442.
    syz:MYO_14470.
    PATRICi23837822. VBISynSp132158_0480.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80485 Genomic DNA. Translation: CAA56652.1 .
    BA000022 Genomic DNA. Translation: BAA17018.1 .
    PIRi S60228.
    RefSeqi NP_440338.1. NC_000911.1.
    YP_005650395.1. NC_017277.1.
    YP_007450221.1. NC_020286.1.

    3D structure databases

    ProteinModelPortali P55037.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P55037. 3 interactions.
    STRINGi 1148.sll1502.

    Proteomic databases

    PaxDbi P55037.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA17018 ; BAA17018 ; BAA17018 .
    GeneIDi 953637.
    KEGGi syn:sll1502.
    syy:SYNGTS_0442.
    syz:MYO_14470.
    PATRICi 23837822. VBISynSp132158_0480.

    Phylogenomic databases

    eggNOGi COG0069.
    HOGENOMi HOG000031559.
    KOi K00284.
    OMAi RRYGHAG.
    OrthoDBi EOG60W7PX.
    PhylomeDBi P55037.

    Enzyme and pathway databases

    UniPathwayi UPA00045 .
    UPA00634 ; UER00691 .

    Family and domain databases

    Gene3Di 2.160.20.60. 1 hit.
    3.20.20.70. 2 hits.
    3.60.20.10. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR002489. Glu_synth_asu_C.
    IPR006982. Glu_synth_centr_N.
    IPR002932. Glu_synthdom.
    IPR029055. Ntn_hydrolases_N.
    [Graphical view ]
    Pfami PF00310. GATase_2. 1 hit.
    PF04898. Glu_syn_central. 1 hit.
    PF01645. Glu_synthase. 1 hit.
    PF01493. GXGXG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    SSF69336. SSF69336. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Existence of two ferredoxin-glutamate synthases in the cyanobacterium Synechocystis sp. PCC 6803. Isolation and insertional inactivation of gltB and gltS genes."
      Navarro F., Chavez S., Candau P., Florencio F.J.
      Plant Mol. Biol. 27:753-767(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.

    Entry informationi

    Entry nameiGLTB_SYNY3
    AccessioniPrimary (citable) accession number: P55037
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-21 is the initiator.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3