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Protein

26S proteasome non-ATPase regulatory subunit 4

Gene

Rpn10

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds and presumably selects ubiquitin-conjugates for destruction.

GO - Molecular functioni

  • polyubiquitin modification-dependent protein binding Source: FlyBase
  • ubiquitin conjugating enzyme binding Source: FlyBase
  • zinc ion binding Source: FlyBase

GO - Biological processi

  • cellular response to DNA damage stimulus Source: FlyBase
  • mitotic sister chromatid segregation Source: FlyBase
  • proteasome assembly Source: GO_Central
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: FlyBase

Enzyme and pathway databases

BRENDAi3.4.25.1. 1994.
ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-209360. Ubiquitination and proteolysis of phosphorylated CI.
R-DME-209406. Degradation of NF-kappa-B inhibitor, CACT.
R-DME-209461. Ubiquitination and degradation of phosphorylated ARM.
R-DME-216167. Nuclear CI is degraded.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-432395. Degradation of TIM.
R-DME-432524. Degradation of PER.
R-DME-446652. Interleukin-1 family signaling.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-538848. Degradation of CLK.
R-DME-538864. Degradation of CRY.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689880. Ub-specific processing proteases.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-8948751. Regulation of PTEN stability and activity.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 4
Alternative name(s):
26S proteasome regulatory subunit RPN10
26S proteasome regulatory subunit S5A
54 kDa subunit of mu particle
Multiubiquitin chain-binding protein
p54
Gene namesi
Name:Rpn10
Synonyms:PROS-54, Pros54
ORF Names:CG7619
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0015283. Rpn10.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738301 – 39626S proteasome non-ATPase regulatory subunit 4Add BLAST396

Proteomic databases

PaxDbiP55035.
PRIDEiP55035.

Expressioni

Gene expression databases

BgeeiFBgn0015283.
ExpressionAtlasiP55035. differential.
GenevisibleiP55035. DM.

Interactioni

Subunit structurei

The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively (By similarity). Interacts with Ubc4.By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi65632. 21 interactors.
DIPiDIP-22777N.
IntActiP55035. 15 interactors.
MINTiMINT-1578442.
STRINGi7227.FBpp0078024.

Structurei

3D structure databases

ProteinModelPortaliP55035.
SMRiP55035.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 188VWFAAdd BLAST188
Domaini212 – 231UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini276 – 295UIM 2PROSITE-ProRule annotationAdd BLAST20
Domaini303 – 322UIM 3PROSITE-ProRule annotationAdd BLAST20

Sequence similaritiesi

Belongs to the proteasome subunit S5A family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2884. Eukaryota.
COG5148. LUCA.
GeneTreeiENSGT00530000064050.
InParanoidiP55035.
KOiK03029.
OMAiMGGKGPE.
OrthoDBiEOG091G0A6L.
PhylomeDBiP55035.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiView protein in InterPro
IPR003903. UIM_dom.
IPR002035. VWF_A.
IPR036465. vWFA_dom_sf.
PfamiView protein in Pfam
PF02809. UIM. 3 hits.
PF13519. VWA_2. 1 hit.
SMARTiView protein in SMART
SM00726. UIM. 3 hits.
SM00327. VWA. 1 hit.
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiView protein in PROSITE
PS50330. UIM. 3 hits.

Sequencei

Sequence statusi: Complete.

P55035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLESTMICF DNSDFQRNGD YFPTRLIVQR DGINLVCLTK LRSNPENNVG
60 70 80 90 100
LMTLSNTVEV LATLTSDAGR IFSKMHLVQP KGEINLLTGI RIAHLVLKHR
110 120 130 140 150
QGKNHKMRIV VFVGSPINHE EGDLVKQAKR LKKEKVNVDI VSFGDHGNNN
160 170 180 190 200
EILTAFINAL NGKDGTGSHL VSVPRGSVLS DALLSSPIIQ GEDGMGGAGL
210 220 230 240 250
GGNVFEFGVD PNEDPELALA LRVSMEEQRQ RQESEQRRAN PDGAPPTGGD
260 270 280 290 300
AGGGGGVSGS GPGNEESAGA ENEANTEEAM LQRALALSTE TPEDNLPDFA
310 320 330 340 350
NMTEEEQIAF AMQMSMQDAP DDSVTQQAKR PKTDEANAPM DVDEDYSEVI
360 370 380 390
GDPAFLQSVL ENLPGVDPQS EAVRDAVGSL NKDKDKKSDG KDSQKK
Length:396
Mass (Da):42,618
Last modified:November 2, 2001 - v2
Checksum:i061418F6F7FE819F
GO

Sequence cautioni

The sequence AAL90071 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9C → S in AAB35145 (PubMed:7649173).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79502 Genomic DNA. Translation: AAB35145.1.
AE014296 Genomic DNA. Translation: AAF51741.1.
AY089333 mRNA. Translation: AAL90071.2. Different initiation.
PIRiS66528.
RefSeqiNP_001262168.1. NM_001275239.1.
NP_524204.1. NM_079480.3.
UniGeneiDm.23542.

Genome annotation databases

EnsemblMetazoaiFBtr0078368; FBpp0078024; FBgn0015283.
FBtr0333453; FBpp0305644; FBgn0015283.
GeneIDi40388.
KEGGidme:Dmel_CG7619.

Similar proteinsi

Entry informationi

Entry nameiPSMD4_DROME
AccessioniPrimary (citable) accession number: P55035
Secondary accession number(s): Q86R87, Q9V473
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 2, 2001
Last modified: November 22, 2017
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families