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Protein

26S proteasome non-ATPase regulatory subunit 4

Gene

Rpn10

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds and presumably selects ubiquitin-conjugates for destruction.

GO - Molecular functioni

  • polyubiquitin binding Source: FlyBase
  • ubiquitin conjugating enzyme binding Source: FlyBase
  • zinc ion binding Source: FlyBase

GO - Biological processi

  • cellular response to DNA damage stimulus Source: FlyBase
  • mitotic sister chromatid segregation Source: FlyBase
  • proteasome assembly Source: GO_Central
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: FlyBase
Complete GO annotation...

Enzyme and pathway databases

BRENDAi3.4.25.1. 1994.
ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-174154. APC/C:Cdc20 mediated degradation of Securin.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2467813. Separation of Sister Chromatids.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5668541. TNFR2 non-canonical NF-kB pathway.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 4
Alternative name(s):
26S proteasome regulatory subunit RPN10
26S proteasome regulatory subunit S5A
54 kDa subunit of mu particle
Multiubiquitin chain-binding protein
p54
Gene namesi
Name:Rpn10
Synonyms:PROS-54, Pros54
ORF Names:CG7619
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0015283. Rpn10.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • cytosol Source: GO_Central
  • nucleus Source: FlyBase
  • proteasome complex Source: FlyBase
  • proteasome regulatory particle Source: FlyBase
  • proteasome regulatory particle, base subcomplex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 39639626S proteasome non-ATPase regulatory subunit 4PRO_0000173830Add
BLAST

Proteomic databases

PaxDbiP55035.

Expressioni

Gene expression databases

BgeeiFBgn0015283.
ExpressionAtlasiP55035. differential.
GenevisibleiP55035. DM.

Interactioni

Subunit structurei

The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively (By similarity). Interacts with Ubc4.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Ubc4P524862EBI-146479,EBI-224571
UbqnQ9VWD94EBI-146479,EBI-100499

GO - Molecular functioni

  • polyubiquitin binding Source: FlyBase
  • ubiquitin conjugating enzyme binding Source: FlyBase

Protein-protein interaction databases

BioGridi65632. 12 interactions.
DIPiDIP-22777N.
IntActiP55035. 15 interactions.
MINTiMINT-1578442.
STRINGi7227.FBpp0078024.

Structurei

3D structure databases

ProteinModelPortaliP55035.
SMRiP55035. Positions 1-192, 199-319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 188188VWFAAdd
BLAST
Domaini212 – 23120UIM 1PROSITE-ProRule annotationAdd
BLAST
Domaini276 – 29520UIM 2PROSITE-ProRule annotationAdd
BLAST
Domaini303 – 32220UIM 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the proteasome subunit S5A family.Curated
Contains 3 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation
Contains 1 VWFA domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2884. Eukaryota.
COG5148. LUCA.
GeneTreeiENSGT00530000064050.
InParanoidiP55035.
KOiK03029.
OMAiDETNAPM.
OrthoDBiEOG091G0A6L.
PhylomeDBiP55035.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR027040. Proteasome_su_Rpn10.
IPR003903. UIM_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10223. PTHR10223. 2 hits.
PfamiPF02809. UIM. 3 hits.
PF13519. VWA_2. 1 hit.
[Graphical view]
SMARTiSM00726. UIM. 3 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50330. UIM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLESTMICF DNSDFQRNGD YFPTRLIVQR DGINLVCLTK LRSNPENNVG
60 70 80 90 100
LMTLSNTVEV LATLTSDAGR IFSKMHLVQP KGEINLLTGI RIAHLVLKHR
110 120 130 140 150
QGKNHKMRIV VFVGSPINHE EGDLVKQAKR LKKEKVNVDI VSFGDHGNNN
160 170 180 190 200
EILTAFINAL NGKDGTGSHL VSVPRGSVLS DALLSSPIIQ GEDGMGGAGL
210 220 230 240 250
GGNVFEFGVD PNEDPELALA LRVSMEEQRQ RQESEQRRAN PDGAPPTGGD
260 270 280 290 300
AGGGGGVSGS GPGNEESAGA ENEANTEEAM LQRALALSTE TPEDNLPDFA
310 320 330 340 350
NMTEEEQIAF AMQMSMQDAP DDSVTQQAKR PKTDEANAPM DVDEDYSEVI
360 370 380 390
GDPAFLQSVL ENLPGVDPQS EAVRDAVGSL NKDKDKKSDG KDSQKK
Length:396
Mass (Da):42,618
Last modified:November 2, 2001 - v2
Checksum:i061418F6F7FE819F
GO

Sequence cautioni

The sequence AAL90071 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91C → S in AAB35145 (PubMed:7649173).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79502 Genomic DNA. Translation: AAB35145.1.
AE014296 Genomic DNA. Translation: AAF51741.1.
AY089333 mRNA. Translation: AAL90071.2. Different initiation.
PIRiS66528.
RefSeqiNP_001262168.1. NM_001275239.1.
NP_524204.1. NM_079480.3.
UniGeneiDm.23542.

Genome annotation databases

EnsemblMetazoaiFBtr0078368; FBpp0078024; FBgn0015283.
FBtr0333453; FBpp0305644; FBgn0015283.
GeneIDi40388.
KEGGidme:Dmel_CG7619.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79502 Genomic DNA. Translation: AAB35145.1.
AE014296 Genomic DNA. Translation: AAF51741.1.
AY089333 mRNA. Translation: AAL90071.2. Different initiation.
PIRiS66528.
RefSeqiNP_001262168.1. NM_001275239.1.
NP_524204.1. NM_079480.3.
UniGeneiDm.23542.

3D structure databases

ProteinModelPortaliP55035.
SMRiP55035. Positions 1-192, 199-319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65632. 12 interactions.
DIPiDIP-22777N.
IntActiP55035. 15 interactions.
MINTiMINT-1578442.
STRINGi7227.FBpp0078024.

Proteomic databases

PaxDbiP55035.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0078368; FBpp0078024; FBgn0015283.
FBtr0333453; FBpp0305644; FBgn0015283.
GeneIDi40388.
KEGGidme:Dmel_CG7619.

Organism-specific databases

CTDi40388.
FlyBaseiFBgn0015283. Rpn10.

Phylogenomic databases

eggNOGiKOG2884. Eukaryota.
COG5148. LUCA.
GeneTreeiENSGT00530000064050.
InParanoidiP55035.
KOiK03029.
OMAiDETNAPM.
OrthoDBiEOG091G0A6L.
PhylomeDBiP55035.

Enzyme and pathway databases

BRENDAi3.4.25.1. 1994.
ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-174154. APC/C:Cdc20 mediated degradation of Securin.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2467813. Separation of Sister Chromatids.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5668541. TNFR2 non-canonical NF-kB pathway.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GenomeRNAii40388.
PROiP55035.

Gene expression databases

BgeeiFBgn0015283.
ExpressionAtlasiP55035. differential.
GenevisibleiP55035. DM.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR027040. Proteasome_su_Rpn10.
IPR003903. UIM_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10223. PTHR10223. 2 hits.
PfamiPF02809. UIM. 3 hits.
PF13519. VWA_2. 1 hit.
[Graphical view]
SMARTiSM00726. UIM. 3 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50330. UIM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSMD4_DROME
AccessioniPrimary (citable) accession number: P55035
Secondary accession number(s): Q86R87, Q9V473
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 2, 2001
Last modified: September 7, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.