Reviewed, UniProtKB/Swiss-Prot P55031 (LIPL_FELCA)

Names and origin

Protein names

Recommended name:
    Lipoprotein lipase
      Short name=LPL
    EC=3.1.1.34

Gene names

Name:

LPL

Organism

Felis catus (Cat) (Felis silvestris catus)

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). The enzyme functions in the presence of apolipoprotein C-2 on the luminal surface of vascular endothelium.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subunit structure	Homodimer. Interacts with apolipoprotein C-2. Interacts with GPIHBP1.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Involvement in disease	Defects in LPL are a cause of chylomicronemia syndrome, also known as type I hyperlipoproteinemia.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cell membrane Chylomicron Membrane VLDL
Disease	Disease mutation Hyperlipidemia
Domain	Signal
Ligand	Heparin-binding
Molecular function	Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell chylomicron Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW very-low-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW lipoprotein lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 478	455	Lipoprotein lipase		PRO_0000017774
Regions
	Domain	344 – 467	124	PLAT
	Region	322 – 334	13	Heparin-binding Potential
Sites
	Active site	162	1	Nucleophile By similarity
	Active site	186	1	Charge relay system By similarity
	Active site	271	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	73	1	N-linked (GlcNAc...) Potential
	Glycosylation	389	1	N-linked (GlcNAc...) Potential
	Disulfide bond	57 ↔ 70		By similarity
	Disulfide bond	246 ↔ 269		By similarity
	Disulfide bond	294 ↔ 313		By similarity
	Disulfide bond	305 ↔ 308		By similarity
	Disulfide bond	448 ↔ 468		By similarity
Natural variations
	Natural variant	435	1	R → G in chylomicronemia syndrome. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P55031-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F57014446127E8C9	FASTA	478	53,668
	10 20 30 40 50 60 MESKTLFLMA LGIWLQSLTT TRGWVAADDR ITGGRDFIDI ESKFALRTPE DIAEDTCHLI 70 80 90 100 110 120 PGVTESVANC HFNHTSKTFV VIHGWTVTGM YESWVPKLVA APYKREPDSN VIVVDWLSRA 130 140 150 160 170 180 QQHYPVSAGY TKLVGKDVAK FINWMAEEFH YPLDNVHLLG YSLGAHAAGI AGSLTNKKVN 190 200 210 220 230 240 RITGLDPAGP NFEYAEAPSR LSPDDADFVD VLHTFTRGSP GRSIGIQKPV GHVDIYPNGG 250 260 270 280 290 300 TFQPGCNIGE AIRVIAERGL GDVDQLVKCS HERSIHLFID SLLNEENPSK AYRCNSKEAF 310 320 330 340 350 360 EKGLCLSCRK NRCNNLGYEI NKVRAKRSSK MYLKTRSQMP YKVFHYQVKI HFSGTESDTQ 370 380 390 400 410 420 TNQVFEISLY GTVAESENIP FTLPEISANK TYSFLIYTEV DIGELLMLKL KWKSDSYFSW 430 440 450 460 470 SDWWSSPGFT IEKIRVKAGE TQKKVIFCSR EKVSHLQKGK ASVVFVKCHD KSLNKKSG

References

[1]

"A mutation in the lipoprotein lipase gene is the molecular basis of chylomicronemia in a colony of domestic cats." Ginzinger D.G., Lewis M.E.S., Ma Y., Jones B.R., Liu G., Jones S.D. J. Clin. Invest. 97:1257-1266(1996) [PubMed: 8636438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT CHYLOMICRONEMIA SYNDROME GLY-435.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U42725 mRNA. Translation: AAB03848.1.
RefSeq	NP_001036032.1.
3D structure databases
HSSP	HSSP built from PDB template 1RP1 based on UniProtKB P06857.
ModBase	Search...
Protein-protein interaction databases
STRING	P55031.
Genome annotation databases
GeneID	727696.
Organism-specific databases
CTD	727696.
Phylogenomic databases
HOVERGEN	P55031.
Enzyme and pathway databases
BRENDA	3.1.1.34. 273949.
Family and domain databases
InterPro	IPR000734. Lipase. IPR013818. Lipase_N. IPR008262. Lipase_Ser_AS. IPR002330. Lipo_Lipase. IPR001024. LipOase_LH2. IPR016272. Lipoprotein_lipase_LIPH. [Graphical view]
PANTHER	PTHR11610. Lipase. 1 hit. PTHR11610:SF3. Lipase_lipo. 1 hit.
Pfam	PF00151. Lipase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PIRSF	PIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTS	PR00822. LIPOLIPASE. PR00821. TAGLIPASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
TIGRFAMs	TIGR03230. lipo_lipase. 1 hit.
PROSITE	PS00120. LIPASE_SER. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIPL_FELCA

Accession

Primary (citable) accession number: P55031

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	October 13, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)