ID   TYRO_ORYLA              Reviewed;         540 AA.
AC   P55025;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   20-JAN-2009, entry version 51.
DE   RecName: Full=Tyrosinase;
DE            EC=1.14.18.1;
DE   AltName: Full=Monophenol monooxygenase;
DE   Flags: Precursor;
GN   Name=tyr; Synonyms=tyro;
OS   Oryzias latipes (Medaka fish) (Japanese ricefish).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Euteleostei; Neoteleostei;
OC   Acanthomorpha; Acanthopterygii; Percomorpha; Atherinomorpha;
OC   Beloniformes; Adrianichthyidae; Oryziinae; Oryzias.
OX   NCBI_TaxID=8090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Eye;
RX   MEDLINE=95121922; PubMed=7821799; DOI=10.1016/0378-1119(94)90445-6;
RA   Inagaki H., Bessho Y., Koga A., Hori H.;
RT   "Expression of the tyrosinase-encoding gene in a colorless melanophore
RT   mutant of the medaka fish, Oryzias latipes.";
RL   Gene 150:319-324(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RA   Inagaki H., Koga A., Bessho Y., Hori H.;
RT   "The tyrosinase gene from medaka: transgenic expression rescued albino
RT   mutation.";
RL   Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: This is a copper-containing oxidase that functions in
CC       the formation of pigments such as melanins and other polyphenolic
CC       compounds.
CC   -!- CATALYTIC ACTIVITY: L-tyrosine + L-dopa + O(2) = L-dopa +
CC       dopaquinone + H(2)O.
CC   -!- COFACTOR: Binds 2 copper ions per subunit.
CC   -!- SUBCELLULAR LOCATION: Melanosome membrane; Single-pass type I
CC       membrane protein.
CC   -!- SIMILARITY: Belongs to the tyrosinase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; D29687; BAA06156.1; -; mRNA.
DR   EMBL; D29686; BAA06155.1; -; mRNA.
DR   EMBL; AB010101; BAA31348.1; -; Genomic_DNA.
DR   RefSeq; NP_001098272.1; -.
DR   UniGene; Ola.86; -.
DR   Ensembl; ENSORLG00000010905; Oryzias latipes.
DR   GeneID; 100049427; -.
DR   HOVERGEN; P55025; -.
DR   BRENDA; 1.14.18.1; 19130.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0033162; C:melanosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005507; F:copper ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004503; F:monophenol monooxygenase activity; IEA:EC.
DR   GO; GO:0006583; P:melanin biosynthetic process from tyrosine; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR008922; Di-copper_centre.
DR   InterPro; IPR002227; Tyrosinase.
DR   Gene3D; G3DSA:1.10.1280.10; Di-copper_centre; 1.
DR   Pfam; PF00264; Tyrosinase; 1.
DR   PRINTS; PR00092; TYROSINASE.
DR   PROSITE; PS00497; TYROSINASE_1; 1.
DR   PROSITE; PS00498; TYROSINASE_2; 1.
PE   2: Evidence at transcript level;
KW   Copper; Glycoprotein; Melanin biosynthesis; Membrane; Metal-binding;
KW   Monooxygenase; Oxidoreductase; Signal; Transmembrane.
FT   SIGNAL        1     19       Potential.
FT   CHAIN        20    540       Tyrosinase.
FT                                /FTId=PRO_0000035883.
FT   TOPO_DOM     20    480       Lumenal, melanosome (Potential).
FT   TRANSMEM    481    501       Potential.
FT   TOPO_DOM    502    540       Cytoplasmic (Potential).
FT   METAL       182    182       Copper A (By similarity).
FT   METAL       205    205       Copper A (By similarity).
FT   METAL       214    214       Copper A (By similarity).
FT   METAL       366    366       Copper B (By similarity).
FT   METAL       370    370       Copper B (By similarity).
FT   METAL       393    393       Copper B (By similarity).
FT   CARBOHYD     87     87       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    233    233       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    293    293       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    340    340       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    374    374       N-linked (GlcNAc...) (Potential).
SQ   SEQUENCE   540 AA;  61254 MW;  7ADEA02C3870CAE4 CRC64;
     MKSLFLSAVL LQFFETCWSQ FPRPCANSEG LRTKECCPVW SGDGSPCGAL SGRGFCADVS
     VSDEPNGPQY PHSGIDDRER WPLAFFNRTC RCAGNYGGFN CGECRFGYWG SNCAEYRESV
     RRNIMSMSTT EQQKFISYLN LAKNTINPDY VITTGTRAEM GENGESPMFS DINTYDLFVW
     IHYYVSRDTF LGGPGNVWRD IDFAHESAAF LPWHRVYLLH WEQEIRKITG DFNFTIPYWD
     WRDAQSCEVC TDNLMGGRNA LNPNLISPAS VFSSWKVICT QQEEYNNQEA LCNATAEGPL
     LRNPGNHDPN RVPRIPTTAD VEFTISLPEY ETGSMDRFAN NSFRNVLEGF ASPETGMAVQ
     GQSTMHNALH VFMNGSMSSV QGSANDPIFL LHHAFIDSIF ERWLRTHQPP RSIYPRTNAP
     IGHNDGYYMV PFLPLYRNGD YLLSNKALGY EYAYLLDPGQ RFVQEFLTPY LQQAQQIWQW
     LLGAGILGAL IATIVAAVIV FARRKRRRNQ KRKRAPSFGE RQPLLQSSSE EGSSSYQTTL
//