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P55025 (TYRO_ORYLA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Tyrosinase
EC=1.14.18.1
Alternative name(s):
Monophenol monooxygenase
Gene names
Name:tyr
Synonyms:tyro
OrganismOryzias latipes (Medaka fish) (Japanese ricefish) [Reference proteome]
Taxonomic identifier8090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Atherinomorpha › Beloniformes › Adrianichthyidae › Oryziinae › Oryzias

Protein attributes

Sequence length540 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.

Catalytic activity

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.

L-tyrosine + O2 = dopaquinone + H2O.

Cofactor

Binds 2 copper ions per subunit.

Subcellular location

Melanosome membrane; Single-pass type I membrane protein.

Sequence similarities

Belongs to the tyrosinase family.

Ontologies

Keywords
   Biological processMelanin biosynthesis
   Cellular componentMembrane
   DomainSignal
Transmembrane
Transmembrane helix
   LigandCopper
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmelanin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

melanosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

monophenol monooxygenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 540521Tyrosinase
PRO_0000035883

Regions

Topological domain20 – 480461Lumenal, melanosome Potential
Transmembrane481 – 50121Helical; Potential
Topological domain502 – 54039Cytoplasmic Potential

Sites

Metal binding1821Copper A By similarity
Metal binding2051Copper A By similarity
Metal binding2141Copper A By similarity
Metal binding3661Copper B By similarity
Metal binding3701Copper B By similarity
Metal binding3931Copper B By similarity

Amino acid modifications

Glycosylation871N-linked (GlcNAc...) Potential
Glycosylation2331N-linked (GlcNAc...) Potential
Glycosylation2931N-linked (GlcNAc...) Potential
Glycosylation3401N-linked (GlcNAc...) Potential
Glycosylation3741N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P55025 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 7ADEA02C3870CAE4

FASTA54061,254
        10         20         30         40         50         60 
MKSLFLSAVL LQFFETCWSQ FPRPCANSEG LRTKECCPVW SGDGSPCGAL SGRGFCADVS 

        70         80         90        100        110        120 
VSDEPNGPQY PHSGIDDRER WPLAFFNRTC RCAGNYGGFN CGECRFGYWG SNCAEYRESV 

       130        140        150        160        170        180 
RRNIMSMSTT EQQKFISYLN LAKNTINPDY VITTGTRAEM GENGESPMFS DINTYDLFVW 

       190        200        210        220        230        240 
IHYYVSRDTF LGGPGNVWRD IDFAHESAAF LPWHRVYLLH WEQEIRKITG DFNFTIPYWD 

       250        260        270        280        290        300 
WRDAQSCEVC TDNLMGGRNA LNPNLISPAS VFSSWKVICT QQEEYNNQEA LCNATAEGPL 

       310        320        330        340        350        360 
LRNPGNHDPN RVPRIPTTAD VEFTISLPEY ETGSMDRFAN NSFRNVLEGF ASPETGMAVQ 

       370        380        390        400        410        420 
GQSTMHNALH VFMNGSMSSV QGSANDPIFL LHHAFIDSIF ERWLRTHQPP RSIYPRTNAP 

       430        440        450        460        470        480 
IGHNDGYYMV PFLPLYRNGD YLLSNKALGY EYAYLLDPGQ RFVQEFLTPY LQQAQQIWQW 

       490        500        510        520        530        540 
LLGAGILGAL IATIVAAVIV FARRKRRRNQ KRKRAPSFGE RQPLLQSSSE EGSSSYQTTL

« Hide

References

[1]"Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes."
Inagaki H., Bessho Y., Koga A., Hori H.
Gene 150:319-324(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Eye.
[2]"The tyrosinase gene from medaka: transgenic expression rescued albino mutation."
Inagaki H., Koga A., Bessho Y., Hori H.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D29687 mRNA. Translation: BAA06156.1.
D29686 mRNA. Translation: BAA06155.1.
AB010101 Genomic DNA. Translation: BAA31348.1.
RefSeqNP_001098272.1. NM_001104802.1.
UniGeneOla.86.

3D structure databases

ProteinModelPortalP55025.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100049427.
KEGGola:100049427.

Organism-specific databases

CTD7299.

Phylogenomic databases

eggNOGNOG08919.
HOGENOMHOG000118376.
HOVERGENHBG003553.
InParanoidP55025.
KOK00505.
OrthoDBEOG4D26PK.

Family and domain databases

Gene3D1.10.1280.10. 1 hit.
InterProIPR002227. Tyrosinase.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamPF00264. Tyrosinase. 1 hit.
[Graphical view]
PRINTSPR00092. TYROSINASE.
SUPFAMSSF48056. Di-copper_centre. 1 hit.
PROSITEPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYRO_ORYLA
AccessionPrimary (citable) accession number: P55025
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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