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Protein

Tyrosinase

Gene

tyr

Organism
Oryzias latipes (Medaka fish) (Japanese ricefish)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.

Catalytic activityi

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
L-tyrosine + O2 = dopaquinone + H2O.

Cofactori

Cu2+Note: Binds 2 copper ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi182 – 1821Copper ABy similarity
Metal bindingi205 – 2051Copper ABy similarity
Metal bindingi214 – 2141Copper ABy similarity
Metal bindingi366 – 3661Copper BBy similarity
Metal bindingi370 – 3701Copper BBy similarity
Metal bindingi393 – 3931Copper BBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. monophenol monooxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. melanin biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosinase (EC:1.14.18.1)
Alternative name(s):
Monophenol monooxygenase
Gene namesi
Name:tyr
Synonyms:tyro
OrganismiOryzias latipes (Medaka fish) (Japanese ricefish)
Taxonomic identifieri8090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaAtherinomorphaeBeloniformesAdrianichthyidaeOryziinaeOryzias
ProteomesiUP000001038 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 480461Lumenal, melanosomeSequence AnalysisAdd
BLAST
Transmembranei481 – 50121HelicalSequence AnalysisAdd
BLAST
Topological domaini502 – 54039CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. melanosome membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 540521TyrosinasePRO_0000035883Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi87 – 871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP55025.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG08919.
HOGENOMiHOG000118376.
HOVERGENiHBG003553.
InParanoidiP55025.
KOiK00505.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF00264. Tyrosinase. 1 hit.
[Graphical view]
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLFLSAVL LQFFETCWSQ FPRPCANSEG LRTKECCPVW SGDGSPCGAL
60 70 80 90 100
SGRGFCADVS VSDEPNGPQY PHSGIDDRER WPLAFFNRTC RCAGNYGGFN
110 120 130 140 150
CGECRFGYWG SNCAEYRESV RRNIMSMSTT EQQKFISYLN LAKNTINPDY
160 170 180 190 200
VITTGTRAEM GENGESPMFS DINTYDLFVW IHYYVSRDTF LGGPGNVWRD
210 220 230 240 250
IDFAHESAAF LPWHRVYLLH WEQEIRKITG DFNFTIPYWD WRDAQSCEVC
260 270 280 290 300
TDNLMGGRNA LNPNLISPAS VFSSWKVICT QQEEYNNQEA LCNATAEGPL
310 320 330 340 350
LRNPGNHDPN RVPRIPTTAD VEFTISLPEY ETGSMDRFAN NSFRNVLEGF
360 370 380 390 400
ASPETGMAVQ GQSTMHNALH VFMNGSMSSV QGSANDPIFL LHHAFIDSIF
410 420 430 440 450
ERWLRTHQPP RSIYPRTNAP IGHNDGYYMV PFLPLYRNGD YLLSNKALGY
460 470 480 490 500
EYAYLLDPGQ RFVQEFLTPY LQQAQQIWQW LLGAGILGAL IATIVAAVIV
510 520 530 540
FARRKRRRNQ KRKRAPSFGE RQPLLQSSSE EGSSSYQTTL
Length:540
Mass (Da):61,254
Last modified:October 1, 1996 - v1
Checksum:i7ADEA02C3870CAE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29687 mRNA. Translation: BAA06156.1.
D29686 mRNA. Translation: BAA06155.1.
AB010101 Genomic DNA. Translation: BAA31348.1.
RefSeqiNP_001098272.1. NM_001104802.1.
UniGeneiOla.86.

Genome annotation databases

GeneIDi100049427.
KEGGiola:100049427.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29687 mRNA. Translation: BAA06156.1.
D29686 mRNA. Translation: BAA06155.1.
AB010101 Genomic DNA. Translation: BAA31348.1.
RefSeqiNP_001098272.1. NM_001104802.1.
UniGeneiOla.86.

3D structure databases

ProteinModelPortaliP55025.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100049427.
KEGGiola:100049427.

Organism-specific databases

CTDi7299.

Phylogenomic databases

eggNOGiNOG08919.
HOGENOMiHOG000118376.
HOVERGENiHBG003553.
InParanoidiP55025.
KOiK00505.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF00264. Tyrosinase. 1 hit.
[Graphical view]
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes."
    Inagaki H., Bessho Y., Koga A., Hori H.
    Gene 150:319-324(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Eye.
  2. "The tyrosinase gene from medaka: transgenic expression rescued albino mutation."
    Inagaki H., Koga A., Bessho Y., Hori H.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiTYRO_ORYLA
AccessioniPrimary (citable) accession number: P55025
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.