Reviewed, UniProtKB/Swiss-Prot P55025 (TYRO_ORYLA)

Names and origin

Protein names

Recommended name:
    Tyrosinase
    EC=1.14.18.1
Alternative name(s):
    Monophenol monooxygenase

Gene names

Name:	tyr
Synonyms:	tyro

Organism

Oryzias latipes (Medaka fish) (Japanese ricefish)

Taxonomic identifier

8090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Atherinomorpha › Beloniformes › Adrianichthyidae › Oryziinae › Oryzias

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.
Catalytic activity	L-tyrosine + L-dopa + O2 = L-dopa + dopaquinone + H2O.
Cofactor	Binds 2 copper ions per subunit.
Subcellular location	Melanosome membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the tyrosinase family.

Ontologies

Keywords
Biological process	Melanin biosynthesis
Cellular component	Membrane
Domain	Signal Transmembrane
Ligand	Copper Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	melanin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW monophenol monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 540	521	Tyrosinase		PRO_0000035883
Regions
	Topological domain	20 – 480	461	Lumenal, melanosome Potential
	Transmembrane	481 – 501	21	Potential
	Topological domain	502 – 540	39	Cytoplasmic Potential
Sites
	Metal binding	182	1	Copper A By similarity
	Metal binding	205	1	Copper A By similarity
	Metal binding	214	1	Copper A By similarity
	Metal binding	366	1	Copper B By similarity
	Metal binding	370	1	Copper B By similarity
	Metal binding	393	1	Copper B By similarity
Amino acid modifications
	Glycosylation	87	1	N-linked (GlcNAc...) Potential
	Glycosylation	233	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	340	1	N-linked (GlcNAc...) Potential
	Glycosylation	374	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P55025-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 7ADEA02C3870CAE4	FASTA	540	61,254
	10 20 30 40 50 60 MKSLFLSAVL LQFFETCWSQ FPRPCANSEG LRTKECCPVW SGDGSPCGAL SGRGFCADVS 70 80 90 100 110 120 VSDEPNGPQY PHSGIDDRER WPLAFFNRTC RCAGNYGGFN CGECRFGYWG SNCAEYRESV 130 140 150 160 170 180 RRNIMSMSTT EQQKFISYLN LAKNTINPDY VITTGTRAEM GENGESPMFS DINTYDLFVW 190 200 210 220 230 240 IHYYVSRDTF LGGPGNVWRD IDFAHESAAF LPWHRVYLLH WEQEIRKITG DFNFTIPYWD 250 260 270 280 290 300 WRDAQSCEVC TDNLMGGRNA LNPNLISPAS VFSSWKVICT QQEEYNNQEA LCNATAEGPL 310 320 330 340 350 360 LRNPGNHDPN RVPRIPTTAD VEFTISLPEY ETGSMDRFAN NSFRNVLEGF ASPETGMAVQ 370 380 390 400 410 420 GQSTMHNALH VFMNGSMSSV QGSANDPIFL LHHAFIDSIF ERWLRTHQPP RSIYPRTNAP 430 440 450 460 470 480 IGHNDGYYMV PFLPLYRNGD YLLSNKALGY EYAYLLDPGQ RFVQEFLTPY LQQAQQIWQW 490 500 510 520 530 540 LLGAGILGAL IATIVAAVIV FARRKRRRNQ KRKRAPSFGE RQPLLQSSSE EGSSSYQTTL

References

[1]	"Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes." Inagaki H., Bessho Y., Koga A., Hori H. Gene 150:319-324(1994) [PubMed: 7821799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Eye.
[2]	"The tyrosinase gene from medaka: transgenic expression rescued albino mutation." Inagaki H., Koga A., Bessho Y., Hori H. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D29687 mRNA. Translation: BAA06156.1. D29686 mRNA. Translation: BAA06155.1. AB010101 Genomic DNA. Translation: BAA31348.1.
RefSeq	NP_001098272.1.
UniGene	Ola.86
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSORLT00000013682; ENSORLP00000013681; ENSORLG00000010905; Oryzias latipes. [Genome view]
GeneID	100049427.
Organism-specific databases
CTD	100049427.
Phylogenomic databases
HOVERGEN	P55025.
Enzyme and pathway databases
BRENDA	1.14.18.1. 19130.
Family and domain databases
InterPro	IPR008922. Di-copper_centre. IPR002227. Tyrosinase. [Graphical view]
Gene3D	G3DSA:1.10.1280.10. Di-copper_centre. 1 hit.
Pfam	PF00264. Tyrosinase. 1 hit. [Graphical view]
PRINTS	PR00092. TYROSINASE.
PROSITE	PS00497. TYROSINASE_1. 1 hit. PS00498. TYROSINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYRO_ORYLA

Accession

Primary (citable) accession number: P55025

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	October 13, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)