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Protein

Solute carrier family 12 member 3

Gene

Slc12a3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Electroneutral sodium and chloride ion cotransporter. In kidney distal convoluted tubules, key mediator of sodium and chloride reabsorption (PubMed:8021284). Receptor for the proinflammatory cytokine IL18. Contributes to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2, either independently of IL18R1, or as a complex with IL18R1 (By similarity).By similarity1 Publication

Enzyme regulationi

Activated by WNK3.By similarity

GO - Molecular functioni

  • enzyme binding Source: RGD
  • sodium:chloride symporter activity Source: RGD
  • sodium ion transmembrane transporter activity Source: UniProtKB
  • transporter activity Source: UniProtKB

GO - Biological processi

  • chloride transport Source: RGD
  • renal sodium ion absorption Source: BHF-UCL
  • sodium ion transmembrane transport Source: BHF-UCL
  • sodium ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Protein family/group databases

TCDBi2.A.30.4.1. the cation-chloride cotransporter (ccc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 12 member 3
Alternative name(s):
Na-Cl symporter
Thiazide-sensitive sodium-chloride cotransporter
Gene namesi
Name:Slc12a3
Synonyms:Tsc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3686. Slc12a3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 133CytoplasmicSequence analysisAdd BLAST133
Transmembranei134 – 154HelicalSequence analysisAdd BLAST21
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 216CytoplasmicSequence analysisAdd BLAST31
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Transmembranei260 – 280HelicalSequence analysisAdd BLAST21
Topological domaini281 – 284CytoplasmicSequence analysis4
Transmembranei285 – 305HelicalSequence analysisAdd BLAST21
Transmembranei338 – 358HelicalSequence analysisAdd BLAST21
Topological domaini359 – 375CytoplasmicSequence analysisAdd BLAST17
Transmembranei376 – 396HelicalSequence analysisAdd BLAST21
Transmembranei451 – 471HelicalSequence analysisAdd BLAST21
Topological domaini472 – 509CytoplasmicSequence analysisAdd BLAST38
Transmembranei510 – 530HelicalSequence analysisAdd BLAST21
Transmembranei531 – 551HelicalSequence analysisAdd BLAST21
Topological domaini552 – 575CytoplasmicSequence analysisAdd BLAST24
Transmembranei576 – 596HelicalSequence analysisAdd BLAST21
Transmembranei659 – 679HelicalSequence analysisAdd BLAST21
Topological domaini680 – 1002CytoplasmicSequence analysisAdd BLAST323

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
  • vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001780281 – 1002Solute carrier family 12 member 3Add BLAST1002

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41PhosphoserineBy similarity1
Modified residuei47PhosphoserineBy similarity1
Modified residuei48PhosphothreonineBy similarity1
Modified residuei53PhosphothreonineBy similarity1
Modified residuei58PhosphothreonineBy similarity1
Modified residuei71PhosphoserineBy similarity1
Modified residuei89PhosphoserineBy similarity1
Modified residuei124PhosphoserineBy similarity1
Glycosylationi404N-linked (GlcNAc...)Sequence analysis1
Glycosylationi424N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Ubiquitinated; ubiquitination is essential for regulation of endocytosis.By similarity
Phosphorylated in response to IL18.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP55018.
PRIDEiP55018.

PTM databases

iPTMnetiP55018.
PhosphoSitePlusiP55018.

Expressioni

Tissue specificityi

Expressed in kidney, predominanty in the outer cortex in short tubule segments.1 Publication

Interactioni

Subunit structurei

Interacts with KLHL3 (By similarity). Interacts with IL18R1; this interaction is increased by IL18 treatment (By similarity).By similarity

GO - Molecular functioni

  • enzyme binding Source: RGD

Protein-protein interaction databases

BioGridi248520. 3 interactors.
STRINGi10116.ENSRNOP00000025377.

Family & Domainsi

Sequence similaritiesi

Belongs to the SLC12A transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2083. Eukaryota.
COG0531. LUCA.
HOGENOMiHOG000062855.
HOVERGENiHBG052851.
InParanoidiP55018.
KOiK14426.
PhylomeDBiP55018.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR013612. AA_permease_N.
IPR018491. SLC12_C.
IPR002948. SLC12A3.
IPR004842. SLC12A_fam.
[Graphical view]
PANTHERiPTHR11827:SF9. PTHR11827:SF9. 2 hits.
PfamiPF00324. AA_permease. 1 hit.
PF08403. AA_permease_N. 1 hit.
PF03522. SLC12. 2 hits.
[Graphical view]
PRINTSiPR01230. NACLTRNSPORT.
TIGRFAMsiTIGR00930. 2a30. 1 hit.

Sequencei

Sequence statusi: Complete.

P55018-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELPVTEMP GDALCSGRFT ISTLLGGDEP PPAACDNSQP SHLTHGSTLY
60 70 80 90 100
LRTFGYNTID VVPAYEHYAN SALPGEPRKV RPTLADLHSF LKQEGSHLHA
110 120 130 140 150
LAFDGRPGHE LTDGLVEDET GANSEKSPGE PVRFGWVKGV MIRCMLNIWG
160 170 180 190 200
VILYLRLPWI TAQAGIVLTW LIILLSVMVT SITGLSISAI STNGKVKSGG
210 220 230 240 250
TYFLISRSLG PELGGSIGLI FAFANAVGVA MHTVGFAETV RDLLQENGTP
260 270 280 290 300
IVDPINDIRI IGVVTVTVLL AISLAGMEWE SKAQVLFFLV IMVSFANYLV
310 320 330 340 350
GTLIPASKDK ASKGFYSYHG DIFVQNLVPD WRGIDGSFFG MFSIFFPSAT
360 370 380 390 400
GILAGANISG DLKDPAVAIP KGTLMAIFWT TISYLAISAT IGSCVVRDAS
410 420 430 440 450
GDVNDTITPG PGLCEGLACG YGWNFTECSQ QHSCRYGLIN YYQTMSMVSA
460 470 480 490 500
FAPLITAGIF GATLSSALAC LVSAAKVFQC LCEDQLYPLI GFFGKGYGKN
510 520 530 540 550
KEPVRGYLLA YAIAVAFIII AELNTIAPII SNFFLCSYAL INFSCFHASI
560 570 580 590 600
TNSPGWRPSF RYYSKWAALF GAVISVVIMF LLTWWAALIA IGVVLFLLLY
610 620 630 640 650
VIYKKPEVNW GSSVQAGSYN LALSYSVGLN EVEDHIKNYR PQCLVLTGPP
660 670 680 690 700
NFRPALVDFV STFTQNLSLM ICGHVLIAPG KQRVPELRLI ASGHTKWLNK
710 720 730 740 750
RKIKAFYSDV IAEDLRSGVQ ILMQASGLGR MKPNILVVGF KKNWQSAHPA
760 770 780 790 800
TLEDYIGILH DAFDFNYGVC IMRMREGLNV SEALQTHTAP EALVQEEQTS
810 820 830 840 850
TIFQSEQGKK TIDIYWLFDD GGLTLLIPYL LHRKKRWGKC KIRVFVGGQI
860 870 880 890 900
NRMDEERKAI ISLLSKFRLG FHEVHVLPDI NQKPQVEHTK RFEDMIAPFR
910 920 930 940 950
LNDGFKDEAT VAEMRRDYPW KISDEEINKN RIKSLRQVRL NEILLDYSRD
960 970 980 990 1000
AALIILTLPI GRKGKCPSSL YMAWLETLSQ DLSPPVLLIR GNQENVLTFY

CQ
Length:1,002
Mass (Da):110,554
Last modified:December 13, 2002 - v2
Checksum:i1EAC70E86D542C9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10097 mRNA. Translation: AAA21252.2.
PIRiB54145.
RefSeqiNP_062218.3. NM_019345.3.
UniGeneiRn.10467.

Genome annotation databases

GeneIDi54300.
KEGGirno:54300.
UCSCiRGD:3686. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10097 mRNA. Translation: AAA21252.2.
PIRiB54145.
RefSeqiNP_062218.3. NM_019345.3.
UniGeneiRn.10467.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248520. 3 interactors.
STRINGi10116.ENSRNOP00000025377.

Protein family/group databases

TCDBi2.A.30.4.1. the cation-chloride cotransporter (ccc) family.

PTM databases

iPTMnetiP55018.
PhosphoSitePlusiP55018.

Proteomic databases

PaxDbiP55018.
PRIDEiP55018.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi54300.
KEGGirno:54300.
UCSCiRGD:3686. rat.

Organism-specific databases

CTDi6559.
RGDi3686. Slc12a3.

Phylogenomic databases

eggNOGiKOG2083. Eukaryota.
COG0531. LUCA.
HOGENOMiHOG000062855.
HOVERGENiHBG052851.
InParanoidiP55018.
KOiK14426.
PhylomeDBiP55018.

Miscellaneous databases

PROiP55018.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR013612. AA_permease_N.
IPR018491. SLC12_C.
IPR002948. SLC12A3.
IPR004842. SLC12A_fam.
[Graphical view]
PANTHERiPTHR11827:SF9. PTHR11827:SF9. 2 hits.
PfamiPF00324. AA_permease. 1 hit.
PF08403. AA_permease_N. 1 hit.
PF03522. SLC12. 2 hits.
[Graphical view]
PRINTSiPR01230. NACLTRNSPORT.
TIGRFAMsiTIGR00930. 2a30. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS12A3_RAT
AccessioniPrimary (citable) accession number: P55018
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 13, 2002
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.