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Protein

Solute carrier family 12 member 2

Gene

Slc12a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.

Enzyme regulationi

Activated by WNK3.By similarity

GO - Molecular functioni

GO - Biological processi

  • ammonium transmembrane transport Source: MGI
  • ammonium transport Source: MGI
  • branching involved in mammary gland duct morphogenesis Source: MGI
  • detection of mechanical stimulus involved in sensory perception of sound Source: MGI
  • mammary duct terminal end bud growth Source: MGI
  • multicellular organism growth Source: MGI
  • potassium ion transport Source: UniProtKB-KW
  • sodium ion transport Source: UniProtKB-KW
  • transepithelial ammonium transport Source: MGI
  • transepithelial chloride transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 12 member 2
Alternative name(s):
Basolateral Na-K-Cl symporter
Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
Gene namesi
Name:Slc12a2
Synonyms:Nkcc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:101924. Slc12a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 278278CytoplasmicSequence analysisAdd
BLAST
Transmembranei279 – 29921HelicalSequence analysisAdd
BLAST
Topological domaini300 – 3023ExtracellularSequence analysis
Transmembranei303 – 32321HelicalSequence analysisAdd
BLAST
Topological domaini324 – 35936CytoplasmicSequence analysisAdd
BLAST
Transmembranei360 – 38021HelicalSequence analysisAdd
BLAST
Topological domaini381 – 40323ExtracellularSequence analysisAdd
BLAST
Transmembranei404 – 42421HelicalSequence analysisAdd
BLAST
Topological domaini425 – 4284CytoplasmicSequence analysis
Transmembranei429 – 44921HelicalSequence analysisAdd
BLAST
Topological domaini450 – 47930ExtracellularSequence analysisAdd
BLAST
Transmembranei480 – 50021HelicalSequence analysisAdd
BLAST
Topological domaini501 – 51313CytoplasmicSequence analysisAdd
BLAST
Transmembranei514 – 53421HelicalSequence analysisAdd
BLAST
Topological domaini535 – 58450ExtracellularSequence analysisAdd
BLAST
Transmembranei585 – 60521HelicalSequence analysisAdd
BLAST
Topological domaini606 – 64439CytoplasmicSequence analysisAdd
BLAST
Transmembranei645 – 66723HelicalSequence analysisAdd
BLAST
Topological domaini668 – 6736ExtracellularSequence analysis
Transmembranei674 – 69118HelicalSequence analysisAdd
BLAST
Topological domaini692 – 70918CytoplasmicSequence analysisAdd
BLAST
Transmembranei710 – 73021HelicalSequence analysisAdd
BLAST
Topological domaini731 – 892162ExtracellularSequence analysisAdd
BLAST
Transmembranei893 – 91321HelicalSequence analysisAdd
BLAST
Topological domaini914 – 1205292CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • basolateral plasma membrane Source: UniProtKB
  • extracellular exosome Source: MGI
  • extracellular vesicle Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12051205Solute carrier family 12 member 2PRO_0000178024Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei74 – 741PhosphoserineBy similarity
Modified residuei76 – 761PhosphoserineBy similarity
Modified residuei259 – 2591PhosphothreonineCombined sources
Glycosylationi544 – 5441N-linked (GlcNAc...); atypical1 Publication
Glycosylationi546 – 5461N-linked (GlcNAc...)1 Publication
Glycosylationi555 – 5551N-linked (GlcNAc...)Sequence analysis
Modified residuei933 – 9331PhosphoserineCombined sources
Modified residuei937 – 9371PhosphoserineCombined sources
Modified residuei987 – 9871PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP55012.
MaxQBiP55012.
PaxDbiP55012.
PRIDEiP55012.

PTM databases

iPTMnetiP55012.
PhosphoSiteiP55012.
SwissPalmiP55012.

Expressioni

Gene expression databases

CleanExiMM_SLC12A2.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Mapk14P478112EBI-621078,EBI-298727
Stk39Q9Z1W93EBI-621078,EBI-444764

Protein-protein interaction databases

BioGridi203277. 1 interaction.
IntActiP55012. 3 interactions.
MINTiMINT-1866676.
STRINGi10090.ENSMUSP00000111023.

Structurei

3D structure databases

ProteinModelPortaliP55012.
SMRiP55012. Positions 340-685.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi87 – 11731Ala-richAdd
BLAST

Sequence similaritiesi

Belongs to the SLC12A transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2083. Eukaryota.
COG0531. LUCA.
HOGENOMiHOG000062855.
HOVERGENiHBG052851.
InParanoidiP55012.
KOiK10951.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR013612. AA_permease_N.
IPR002443. Na/K/Cl_cotranspt.
IPR002444. NKCC1.
IPR018491. SLC12_C.
IPR004842. SLC12A_fam.
[Graphical view]
PANTHERiPTHR11827:SF58. PTHR11827:SF58. 3 hits.
PfamiPF00324. AA_permease. 1 hit.
PF08403. AA_permease_N. 1 hit.
PF03522. SLC12. 1 hit.
[Graphical view]
PRINTSiPR01207. NAKCLTRNSPRT.
PR01208. NAKCLTRSPRT1.
TIGRFAMsiTIGR00930. 2a30. 1 hit.

Sequencei

Sequence statusi: Complete.

P55012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPGPARPRL APAARPGWGR AAGCRRRGGP ARHGRASGQE DATTAGRQAG
60 70 80 90 100
GGVRGEGTPA AGDGLGRPLG PTPSQSRFQV DPVSENAGRA AAAAAAAAAA
110 120 130 140 150
AAAAGAAGKE TPAAGKAGGE SGVAKGSEEA KGRFRVNFVD PAASSSADDS
160 170 180 190 200
LSDAAGVGGD GPNVSFQNGG DTVLSEGSSL HSGGGSGHHQ QYYYDTHTNT
210 220 230 240 250
YYLRTFGHNT MDAVPRIDHY RHTAAQLGEK LLRPSLAELH DELEKEPFED
260 270 280 290 300
GFANGEESTP TRDAVVAYTA ESKGVVKFGW IKGVLVRCML NIWGVMLFIR
310 320 330 340 350
LSWIVGQAGI GLSVVVIAMA TVVTTITGLS TSAIATNGFV RGGGAYYLIS
360 370 380 390 400
RSLGPEFGGA IGLIFAFANA VAVAMYVVGF AETVVELLKE HSILMIDEIN
410 420 430 440 450
DIRIIGAITV VILLGISVAG MEWEAKAQIV LLVILLLAIA DFVIGTFISL
460 470 480 490 500
ESKKPKGFFG YKSEIFNENF GPDFREEETF FSVFAIFFPA ATGILAGANI
510 520 530 540 550
SGDLADPQSA IPKGTLLAIL ITTVVYIGIA VSVGSCVVRD ATGNVNDTIT
560 570 580 590 600
TELTNCTSAA CKLNFDFSYC ESNTCSYGLM NNFQVMSMVS GFAPLISAGI
610 620 630 640 650
FSATLSSALA SLVSAPKIFQ ALCKDNIYPA FQMFAKGYGK NNEPLRGYIL
660 670 680 690 700
TFLIALGFIL IAELNVIAPI ISNFFLASYA LINFSVFHAS LAKSPGWRPA
710 720 730 740 750
FKYYNMWISL IGAILCCIVM FVINWWAALL TYVIVLGLYI YVTYKKPDVN
760 770 780 790 800
WGSSTQALTY LSALQHSIRL SGVEDHVKNF RPQCLVMTGS PNSRPALLHL
810 820 830 840 850
VHDFTKNVGL MICGHVHMGP RRQAMKEMSI DQARYQRWLI KNKMKAFYAP
860 870 880 890 900
VHADDLREGA QYLMQAAGLG RMKPNTLVLG FKKDWLQADM RDVDMYINLF
910 920 930 940 950
HDAFDIQFGV VVIRLKEGLD ISHLQGQEEL LSSQEKSPGT KDVVVNVDYS
960 970 980 990 1000
KKSDQDTCKS SGEKSITQKD EEEDGKTPTQ PLLKKESKGP IVPLNVADQK
1010 1020 1030 1040 1050
LLEASTQFQK KQGKNTIDVW WLFDDGGLTL LIPYLLTTKK KWKDCKIRVF
1060 1070 1080 1090 1100
IGGKINRIDH DRRAMATLLS KFRIDFSDIM VLGDINTKPK KENIIAFDDM
1110 1120 1130 1140 1150
IEPYRLHEDD KEQDIADKMK EDEPWRITDN ELELYKTKTY RQIRLNELLK
1160 1170 1180 1190 1200
EHSSTANIIV MSLPVARKGA VSSALYMAWL EALSKDLPPV LLVRGNHQSV

LTFYS
Length:1,205
Mass (Da):131,034
Last modified:July 27, 2011 - v2
Checksum:i4DE24CC8070D42F2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71R → G in AAC77832 (PubMed:7929272).Curated
Sequence conflicti1097 – 10971F → Y in AAC77832 (PubMed:7929272).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13174 mRNA. Translation: AAC77832.1.
AC127678 Genomic DNA. No translation available.
PIRiA55015.
RefSeqiNP_033220.2. NM_009194.3.
UniGeneiMm.399997.

Genome annotation databases

GeneIDi20496.
KEGGimmu:20496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13174 mRNA. Translation: AAC77832.1.
AC127678 Genomic DNA. No translation available.
PIRiA55015.
RefSeqiNP_033220.2. NM_009194.3.
UniGeneiMm.399997.

3D structure databases

ProteinModelPortaliP55012.
SMRiP55012. Positions 340-685.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203277. 1 interaction.
IntActiP55012. 3 interactions.
MINTiMINT-1866676.
STRINGi10090.ENSMUSP00000111023.

PTM databases

iPTMnetiP55012.
PhosphoSiteiP55012.
SwissPalmiP55012.

Proteomic databases

EPDiP55012.
MaxQBiP55012.
PaxDbiP55012.
PRIDEiP55012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20496.
KEGGimmu:20496.

Organism-specific databases

CTDi6558.
MGIiMGI:101924. Slc12a2.

Phylogenomic databases

eggNOGiKOG2083. Eukaryota.
COG0531. LUCA.
HOGENOMiHOG000062855.
HOVERGENiHBG052851.
InParanoidiP55012.
KOiK10951.

Miscellaneous databases

ChiTaRSiSlc12a2. mouse.
NextBioi298651.
PROiP55012.
SOURCEiSearch...

Gene expression databases

CleanExiMM_SLC12A2.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR013612. AA_permease_N.
IPR002443. Na/K/Cl_cotranspt.
IPR002444. NKCC1.
IPR018491. SLC12_C.
IPR004842. SLC12A_fam.
[Graphical view]
PANTHERiPTHR11827:SF58. PTHR11827:SF58. 3 hits.
PfamiPF00324. AA_permease. 1 hit.
PF08403. AA_permease_N. 1 hit.
PF03522. SLC12. 1 hit.
[Graphical view]
PRINTSiPR01207. NAKCLTRNSPRT.
PR01208. NAKCLTRSPRT1.
TIGRFAMsiTIGR00930. 2a30. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and chromosome localization of a putative basolateral Na(+)-K(+)-2Cl-cotransporter from mouse inner medullary collecting duct (mIMCD-3) cells."
    Delpire E., Rauchman M.I., Beier D.R., Hebert S.C., Gullans S.R.
    J. Biol. Chem. 269:25677-25683(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-259 AND SER-933, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-544 AND ASN-546.
    Tissue: Myoblast.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-937 AND SER-987, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiS12A2_MOUSE
AccessioniPrimary (citable) accession number: P55012
Secondary accession number(s): E9PYU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: March 16, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.