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Protein

Eukaryotic translation initiation factor 5

Gene

EIF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 34GTPSequence analysis8

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • GTPase activity Source: ProtInc
  • GTP binding Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB
  • translation factor activity, RNA binding Source: ProtInc
  • translation initiation factor activity Source: UniProtKB

GO - Biological processi

  • activation of GTPase activity Source: Ensembl
  • formation of translation preinitiation complex Source: Ensembl
  • regulation of translational initiation Source: CACAO
  • translational initiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100664-MONOMER.
ReactomeiR-HSA-72702. Ribosomal scanning and start codon recognition.
R-HSA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
SIGNORiP55010.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5
Short name:
eIF-5
Gene namesi
Name:EIF5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:3299. EIF5.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB
  • plasma membrane Source: HPA
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi1983.
OpenTargetsiENSG00000100664.
PharmGKBiPA27725.

Polymorphism and mutation databases

BioMutaiEIF5.
DMDMi27735202.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125161 – 431Eukaryotic translation initiation factor 5Add BLAST431

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineCombined sources1
Modified residuei227PhosphothreonineCombined sources1
Modified residuei229PhosphoserineCombined sources1
Modified residuei389PhosphoserineCombined sources1
Modified residuei390PhosphoserineCombined sources1
Modified residuei410PhosphoserineCombined sources1
Modified residuei419PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP55010.
MaxQBiP55010.
PaxDbiP55010.
PeptideAtlasiP55010.
PRIDEiP55010.
TopDownProteomicsiP55010.

PTM databases

iPTMnetiP55010.
PhosphoSitePlusiP55010.
SwissPalmiP55010.

Expressioni

Gene expression databases

BgeeiENSG00000100664.
CleanExiHS_EIF5.
ExpressionAtlasiP55010. baseline and differential.
GenevisibleiP55010. HS.

Organism-specific databases

HPAiCAB004226.
HPA000867.

Interactioni

Subunit structurei

Interacts with FMR1 isoform 6; this interaction occurs in a RNA-dependent manner.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DUSP12Q9UNI65EBI-286450,EBI-715161

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

Protein-protein interaction databases

BioGridi108298. 56 interactors.
IntActiP55010. 9 interactors.
MINTiMINT-5004352.
STRINGi9606.ENSP00000216554.

Structurei

Secondary structure

1431
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Beta strandi17 – 19Combined sources3
Beta strandi23 – 25Combined sources3
Beta strandi27 – 31Combined sources5
Beta strandi33 – 36Combined sources4
Helixi39 – 45Combined sources7
Helixi51 – 61Combined sources11
Beta strandi65 – 68Combined sources4
Turni69 – 72Combined sources4
Beta strandi73 – 78Combined sources6
Helixi82 – 95Combined sources14
Turni100 – 102Combined sources3
Beta strandi103 – 105Combined sources3
Beta strandi108 – 112Combined sources5
Turni113 – 116Combined sources4
Beta strandi117 – 121Combined sources5
Beta strandi123 – 125Combined sources3
Beta strandi128 – 130Combined sources3
Beta strandi133 – 136Combined sources4
Helixi137 – 143Combined sources7
Helixi236 – 252Combined sources17
Helixi256 – 258Combined sources3
Helixi260 – 269Combined sources10
Helixi273 – 275Combined sources3
Helixi276 – 284Combined sources9
Helixi289 – 295Combined sources7
Helixi297 – 304Combined sources8
Helixi308 – 324Combined sources17
Helixi326 – 329Combined sources4
Helixi330 – 332Combined sources3
Helixi333 – 342Combined sources10
Helixi348 – 356Combined sources9
Beta strandi361 – 363Combined sources3
Helixi365 – 383Combined sources19
Helixi400 – 408Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E9HNMR-A1-150[»]
2G2KNMR-A2-170[»]
2IU1X-ray1.80A232-431[»]
ProteinModelPortaliP55010.
SMRiP55010.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55010.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini233 – 392W2PROSITE-ProRule annotationAdd BLAST160

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi196 – 202Asp/Glu-rich (highly acidic)7
Compositional biasi384 – 402Asp/Glu-rich (highly acidic)Add BLAST19
Compositional biasi423 – 429Asp-rich (acidic)7

Sequence similaritiesi

Belongs to the eIF-2-beta/eIF-5 family.Curated
Contains 1 W2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2767. Eukaryota.
COG1601. LUCA.
GeneTreeiENSGT00390000016478.
HOGENOMiHOG000214327.
HOVERGENiHBG006132.
InParanoidiP55010.
KOiK03262.
OMAiPPHTMEE.
OrthoDBiEOG091G0AKW.
PhylomeDBiP55010.
TreeFamiTF101533.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.30.30.50. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR016021. MIF4-like.
IPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
IPR003307. W2_domain.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
SM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF48371. SSF48371. 1 hit.
SSF75689. SSF75689. 1 hit.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P55010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVNVNRSVS DQFYRYKMPR LIAKVEGKGN GIKTVIVNMV DVAKALNRPP
60 70 80 90 100
TYPTKYFGCE LGAQTQFDVK NDRYIVNGSH EANKLQDMLD GFIKKFVLCP
110 120 130 140 150
ECENPETDLH VNPKKQTIGN SCKACGYRGM LDTHHKLCTF ILKNPPENSD
160 170 180 190 200
SGTGKKEKEK KNRKGKDKEN GSVSSSETPP PPPPPNEINP PPHTMEEEED
210 220 230 240 250
DDWGEDTTEE AQRRRMDEIS DHAKVLTLSD DLERTIEERV NILFDFVKKK
260 270 280 290 300
KEEGVIDSSD KEIVAEAERL DVKAMGPLVL TEVLFNEKIR EQIKKYRRHF
310 320 330 340 350
LRFCHNNKKA QRYLLHGLEC VVAMHQAQLI SKIPHILKEM YDADLLEEEV
360 370 380 390 400
IISWSEKASK KYVSKELAKE IRVKAEPFIK WLKEAEEESS GGEEEDEDEN
410 420 430
IEVVYSKAAS VPKVETVKSD NKDDDIDIDA I
Length:431
Mass (Da):49,223
Last modified:January 10, 2003 - v2
Checksum:iC6CCC3A255F9B9BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60E → G in CAB45711 (PubMed:11230166).Curated1
Sequence conflicti203W → S in AAC50572 (PubMed:8663286).Curated1
Sequence conflicti295K → E in CAB45711 (PubMed:11230166).Curated1
Sequence conflicti311Q → K in AAH32866 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_036467418K → M in a breast cancer sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49436 mRNA. Translation: AAC50572.1.
AL080102 mRNA. Translation: CAB45711.1.
AK026933 mRNA. Translation: BAB15593.1.
BX537367 mRNA. Translation: CAD97610.1.
CH471061 Genomic DNA. Translation: EAW81809.1.
BC007728 mRNA. Translation: AAH07728.1.
BC032866 mRNA. Translation: AAH32866.1.
CCDSiCCDS9980.1.
PIRiT12450.
RefSeqiNP_001960.2. NM_001969.4.
NP_892116.2. NM_183004.4.
UniGeneiHs.433702.
Hs.741278.

Genome annotation databases

EnsembliENST00000216554; ENSP00000216554; ENSG00000100664.
ENST00000392715; ENSP00000376477; ENSG00000100664.
ENST00000558506; ENSP00000453743; ENSG00000100664.
GeneIDi1983.
KEGGihsa:1983.
UCSCiuc001ymq.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49436 mRNA. Translation: AAC50572.1.
AL080102 mRNA. Translation: CAB45711.1.
AK026933 mRNA. Translation: BAB15593.1.
BX537367 mRNA. Translation: CAD97610.1.
CH471061 Genomic DNA. Translation: EAW81809.1.
BC007728 mRNA. Translation: AAH07728.1.
BC032866 mRNA. Translation: AAH32866.1.
CCDSiCCDS9980.1.
PIRiT12450.
RefSeqiNP_001960.2. NM_001969.4.
NP_892116.2. NM_183004.4.
UniGeneiHs.433702.
Hs.741278.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E9HNMR-A1-150[»]
2G2KNMR-A2-170[»]
2IU1X-ray1.80A232-431[»]
ProteinModelPortaliP55010.
SMRiP55010.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108298. 56 interactors.
IntActiP55010. 9 interactors.
MINTiMINT-5004352.
STRINGi9606.ENSP00000216554.

PTM databases

iPTMnetiP55010.
PhosphoSitePlusiP55010.
SwissPalmiP55010.

Polymorphism and mutation databases

BioMutaiEIF5.
DMDMi27735202.

Proteomic databases

EPDiP55010.
MaxQBiP55010.
PaxDbiP55010.
PeptideAtlasiP55010.
PRIDEiP55010.
TopDownProteomicsiP55010.

Protocols and materials databases

DNASUi1983.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216554; ENSP00000216554; ENSG00000100664.
ENST00000392715; ENSP00000376477; ENSG00000100664.
ENST00000558506; ENSP00000453743; ENSG00000100664.
GeneIDi1983.
KEGGihsa:1983.
UCSCiuc001ymq.5. human.

Organism-specific databases

CTDi1983.
DisGeNETi1983.
GeneCardsiEIF5.
HGNCiHGNC:3299. EIF5.
HPAiCAB004226.
HPA000867.
MIMi601710. gene.
neXtProtiNX_P55010.
OpenTargetsiENSG00000100664.
PharmGKBiPA27725.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2767. Eukaryota.
COG1601. LUCA.
GeneTreeiENSGT00390000016478.
HOGENOMiHOG000214327.
HOVERGENiHBG006132.
InParanoidiP55010.
KOiK03262.
OMAiPPHTMEE.
OrthoDBiEOG091G0AKW.
PhylomeDBiP55010.
TreeFamiTF101533.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100664-MONOMER.
ReactomeiR-HSA-72702. Ribosomal scanning and start codon recognition.
R-HSA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
SIGNORiP55010.

Miscellaneous databases

ChiTaRSiEIF5. human.
EvolutionaryTraceiP55010.
GeneWikiiEIF5.
GenomeRNAii1983.
PROiP55010.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100664.
CleanExiHS_EIF5.
ExpressionAtlasiP55010. baseline and differential.
GenevisibleiP55010. HS.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.30.30.50. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR016021. MIF4-like.
IPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
IPR003307. W2_domain.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
SM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF48371. SSF48371. 1 hit.
SSF75689. SSF75689. 1 hit.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF5_HUMAN
AccessioniPrimary (citable) accession number: P55010
Secondary accession number(s): Q53XB3, Q9H5N2, Q9UG48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 10, 2003
Last modified: November 30, 2016
This is version 167 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Translation initiation factors
    List of translation initiation factor entries
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.