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Protein

Allograft inflammatory factor 1

Gene

Aif1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play an role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi58 – 69121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi94 – 105122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • actin filament bundle assembly Source: UniProtKB
  • actin filament polymerization Source: UniProtKB
  • cellular response to extracellular stimulus Source: RGD
  • cellular response to hormone stimulus Source: RGD
  • cellular response to hydroperoxide Source: RGD
  • cellular response to interferon-gamma Source: UniProtKB
  • cellular response to morphine Source: RGD
  • inflammatory response Source: UniProtKB
  • macrophage activation Source: UniProtKB
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of gene expression Source: RGD
  • negative regulation of smooth muscle cell chemotaxis Source: Ensembl
  • negative regulation of smooth muscle cell proliferation Source: Ensembl
  • phagocytosis, engulfment Source: UniProtKB
  • positive regulation of cell migration Source: RGD
  • positive regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
  • positive regulation of monocyte chemotaxis Source: UniProtKB
  • positive regulation of muscle hyperplasia Source: RGD
  • positive regulation of nitric oxide biosynthetic process Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • positive regulation of smooth muscle cell chemotaxis Source: UniProtKB
  • positive regulation of smooth muscle cell proliferation Source: UniProtKB
  • positive regulation of T cell migration Source: UniProtKB
  • positive regulation of T cell proliferation Source: UniProtKB
  • Rac protein signal transduction Source: UniProtKB
  • response to axon injury Source: RGD
  • response to cytokine Source: RGD
  • response to electrical stimulus Source: RGD
  • response to glucocorticoid Source: RGD
  • ruffle assembly Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Allograft inflammatory factor 1
Short name:
AIF-1
Alternative name(s):
Ionized calcium-binding adapter molecule 1
MRF-1
Microglia response factor
Gene namesi
Name:Aif1
Synonyms:Iba1, Mrf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi61924. Aif1.

Subcellular locationi

  • Cytoplasmcytoskeleton By similarity
  • Cell projectionruffle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Cell projectionphagocytic cup By similarity

  • Note: Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.By similarity

GO - Cellular componenti

  • actin filament Source: Ensembl
  • cell projection Source: RGD
  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB
  • lamellipodium Source: UniProtKB
  • nucleus Source: UniProtKB
  • perikaryon Source: RGD
  • perinuclear region of cytoplasm Source: Ensembl
  • phagocytic cup Source: UniProtKB
  • ruffle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 147146Allograft inflammatory factor 1PRO_0000073869Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei11 – 111N6-acetyllysineBy similarity
Modified residuei39 – 391PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP55009.

PTM databases

iPTMnetiP55009.
PhosphoSiteiP55009.

Expressioni

Tissue specificityi

Cardiac allograft, spleen and testis. Expressed by inflammatory cells (macrophages and neutrophils).

Developmental stagei

Expressed early and persistently in chronically rejecting cardiac allografts but is absent in cardiac syngrafts and host hearts. In the embryonic cerebellum, expression peaks at day 7.

Inductioni

By interferon gamma.

Gene expression databases

BgeeiENSRNOG00000000853.
ExpressionAtlasiP55009. baseline and differential.
GenevisibleiP55009. RN.

Interactioni

Subunit structurei

Homodimer (Potential). Monomer. Interacts with LCP1 (By similarity).By similarityCurated

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliP55009.
SMRiP55009. Positions 2-147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 8036EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini81 – 11535EF-hand 2; degeneratePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000013846.
HOGENOMiHOG000231928.
HOVERGENiHBG004002.
InParanoidiP55009.
KOiK18617.
OMAiAFKKKYM.
OrthoDBiEOG091G0ZI9.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55009-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSKDLQGG KAFGLLKAQQ EERLDGINKH FLDDPKYSSD EDLQSKLEAF
60 70 80 90 100
KTKYMEFDLN GNGDIDIMSL KRMLEKLGVP KTHLELKKLI REVSSGSEET
110 120 130 140
FSYSDFLRMM LGKRSAILRM ILMYEEKNKE HQKPTGPPAK KAISELP
Length:147
Mass (Da):16,827
Last modified:October 1, 1996 - v1
Checksum:i76EA1DAC977A71DA
GO

Sequence cautioni

The sequence AAB06590 differs from that shown.Intron retention.Curated
The sequence AAB06590 differs from that shown. Reason: Frameshift at position 130. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 98SQSKDLQG → MKPEEISR in BAA11533 (PubMed:8713135).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17919 mRNA. Translation: AAA80105.1.
U33471 mRNA. Translation: AAB06590.1. Sequence problems.
D82069 mRNA. Translation: BAA11533.1.
AB000818 mRNA. Translation: BAA19189.1.
BX883046 Genomic DNA. Translation: CAE83999.1.
PIRiI55617.
JC4902.
RefSeqiNP_058892.1. NM_017196.3.
XP_006256127.1. XM_006256065.2.
UniGeneiRn.32080.

Genome annotation databases

EnsembliENSRNOT00000001135; ENSRNOP00000001135; ENSRNOG00000000853.
GeneIDi29427.
KEGGirno:29427.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17919 mRNA. Translation: AAA80105.1.
U33471 mRNA. Translation: AAB06590.1. Sequence problems.
D82069 mRNA. Translation: BAA11533.1.
AB000818 mRNA. Translation: BAA19189.1.
BX883046 Genomic DNA. Translation: CAE83999.1.
PIRiI55617.
JC4902.
RefSeqiNP_058892.1. NM_017196.3.
XP_006256127.1. XM_006256065.2.
UniGeneiRn.32080.

3D structure databases

ProteinModelPortaliP55009.
SMRiP55009. Positions 2-147.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP55009.
PhosphoSiteiP55009.

Proteomic databases

PRIDEiP55009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001135; ENSRNOP00000001135; ENSRNOG00000000853.
GeneIDi29427.
KEGGirno:29427.

Organism-specific databases

CTDi199.
RGDi61924. Aif1.

Phylogenomic databases

GeneTreeiENSGT00390000013846.
HOGENOMiHOG000231928.
HOVERGENiHBG004002.
InParanoidiP55009.
KOiK18617.
OMAiAFKKKYM.
OrthoDBiEOG091G0ZI9.

Miscellaneous databases

PROiP55009.

Gene expression databases

BgeeiENSRNOG00000000853.
ExpressionAtlasiP55009. baseline and differential.
GenevisibleiP55009. RN.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAIF1_RAT
AccessioniPrimary (citable) accession number: P55009
Secondary accession number(s): P55007, P70491
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.