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Protein

Allograft inflammatory factor 1

Gene

Aif1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play an role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi58 – 691PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi94 – 1052PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • actin filament bundle assembly Source: UniProtKB
  • actin filament polymerization Source: UniProtKB
  • cellular response to extracellular stimulus Source: RGD
  • cellular response to hormone stimulus Source: RGD
  • cellular response to hydroperoxide Source: RGD
  • cellular response to interferon-gamma Source: UniProtKB
  • cellular response to morphine Source: RGD
  • inflammatory response Source: UniProtKB
  • macrophage activation Source: UniProtKB
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of gene expression Source: RGD
  • negative regulation of smooth muscle cell chemotaxis Source: Ensembl
  • negative regulation of smooth muscle cell proliferation Source: Ensembl
  • phagocytosis, engulfment Source: UniProtKB
  • positive regulation of cell migration Source: RGD
  • positive regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
  • positive regulation of monocyte chemotaxis Source: UniProtKB
  • positive regulation of muscle hyperplasia Source: RGD
  • positive regulation of nitric oxide biosynthetic process Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • positive regulation of smooth muscle cell chemotaxis Source: UniProtKB
  • positive regulation of smooth muscle cell proliferation Source: RGD
  • positive regulation of T cell migration Source: UniProtKB
  • positive regulation of T cell proliferation Source: UniProtKB
  • Rac protein signal transduction Source: UniProtKB
  • response to axon injury Source: RGD
  • response to cytokine Source: RGD
  • response to electrical stimulus Source: RGD
  • response to glucocorticoid Source: RGD
  • ruffle assembly Source: UniProtKB

Keywordsi

Molecular functionActin-binding
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Allograft inflammatory factor 1
Short name:
AIF-1
Alternative name(s):
Ionized calcium-binding adapter molecule 1
MRF-1
Microglia response factor
Gene namesi
Name:Aif1
Synonyms:Iba1, Mrf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi61924 Aif1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000738692 – 147Allograft inflammatory factor 1Add BLAST146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei11N6-acetyllysineBy similarity1
Modified residuei39PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP55009
PRIDEiP55009

PTM databases

iPTMnetiP55009
PhosphoSitePlusiP55009

Expressioni

Tissue specificityi

Cardiac allograft, spleen and testis. Expressed by inflammatory cells (macrophages and neutrophils).

Developmental stagei

Expressed early and persistently in chronically rejecting cardiac allografts but is absent in cardiac syngrafts and host hearts. In the embryonic cerebellum, expression peaks at day 7.

Inductioni

By interferon gamma.

Gene expression databases

BgeeiENSRNOG00000000853
ExpressionAtlasiP55009 baseline and differential
GenevisibleiP55009 RN

Interactioni

Subunit structurei

Homodimer (Potential). Monomer. Interacts with LCP1 (By similarity).By similarityCurated

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001138

Structurei

3D structure databases

ProteinModelPortaliP55009
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 80EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini81 – 115EF-hand 2; degeneratePROSITE-ProRule annotationAdd BLAST35

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000013846
HOGENOMiHOG000231928
HOVERGENiHBG004002
InParanoidiP55009
KOiK18617
OMAiAFKKKYM
OrthoDBiEOG091G0ZI9

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR002048 EF_hand_dom
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS50222 EF_HAND_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55009-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSKDLQGG KAFGLLKAQQ EERLDGINKH FLDDPKYSSD EDLQSKLEAF
60 70 80 90 100
KTKYMEFDLN GNGDIDIMSL KRMLEKLGVP KTHLELKKLI REVSSGSEET
110 120 130 140
FSYSDFLRMM LGKRSAILRM ILMYEEKNKE HQKPTGPPAK KAISELP
Length:147
Mass (Da):16,827
Last modified:October 1, 1996 - v1
Checksum:i76EA1DAC977A71DA
GO

Sequence cautioni

The sequence AAB06590 differs from that shown. Intron retention.Curated
The sequence AAB06590 differs from that shown. Reason: Frameshift at position 130.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 9SQSKDLQG → MKPEEISR in BAA11533 (PubMed:8713135).Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17919 mRNA Translation: AAA80105.1
U33471 mRNA Translation: AAB06590.1 Sequence problems.
D82069 mRNA Translation: BAA11533.1
AB000818 mRNA Translation: BAA19189.1
BX883046 Genomic DNA Translation: CAE83999.1
PIRiI55617
JC4902
RefSeqiNP_058892.1, NM_017196.3
XP_006256127.1, XM_006256065.2
UniGeneiRn.32080

Genome annotation databases

EnsembliENSRNOT00000001135; ENSRNOP00000001135; ENSRNOG00000000853
GeneIDi29427
KEGGirno:29427

Similar proteinsi

Entry informationi

Entry nameiAIF1_RAT
AccessioniPrimary (citable) accession number: P55009
Secondary accession number(s): P55007, P70491
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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