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Protein

Allograft inflammatory factor 1

Gene

AIF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi58 – 691PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi94 – 1052PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • actin filament binding Source: UniProtKB
  • calcium ion binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Allograft inflammatory factor 1
Short name:
AIF-1
Alternative name(s):
Ionized calcium-binding adapter molecule 1
Protein G1
Gene namesi
Name:AIF1
Synonyms:G1, IBA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:352. AIF1.

Subcellular locationi

  • Cytoplasmcytoskeleton By similarity
  • Cell projectionruffle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Cell projectionphagocytic cup By similarity

  • Note: Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.By similarity

GO - Cellular componenti

  • actin filament Source: Ensembl
  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB
  • lamellipodium Source: UniProtKB
  • nucleus Source: UniProtKB
  • perikaryon Source: Ensembl
  • perinuclear region of cytoplasm Source: UniProtKB
  • phagocytic cup Source: UniProtKB
  • ruffle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi199.
OpenTargetsiENSG00000204472.
ENSG00000206428.
ENSG00000234836.
ENSG00000235588.
ENSG00000235985.
ENSG00000237727.
PharmGKBiPA24646.

Polymorphism and mutation databases

BioMutaiAIF1.
DMDMi1703217.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000738652 – 147Allograft inflammatory factor 1Add BLAST146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei11N6-acetyllysineCombined sources1
Modified residuei39PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on serine residues.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP55008.
PaxDbiP55008.
PeptideAtlasiP55008.
PRIDEiP55008.
TopDownProteomicsiP55008-1. [P55008-1]

PTM databases

iPTMnetiP55008.
PhosphoSitePlusiP55008.

Expressioni

Tissue specificityi

Detected in T-lymphocytes and peripheral blood mononuclear cells.1 Publication

Gene expression databases

BgeeiENSG00000204472.
CleanExiHS_AIF1.
ExpressionAtlasiP55008. baseline and differential.
GenevisibleiP55008. HS.

Organism-specific databases

HPAiHPA049234.

Interactioni

Subunit structurei

Homodimer (Potential). Monomer. Interacts with LCP1.Curated2 Publications

GO - Molecular functioni

  • actin filament binding Source: UniProtKB

Protein-protein interaction databases

BioGridi106702. 1 interactor.
IntActiP55008. 2 interactors.
STRINGi9606.ENSP00000365227.

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 13Combined sources4
Helixi20 – 32Combined sources13
Helixi35 – 37Combined sources3
Helixi43 – 54Combined sources12
Helixi67 – 76Combined sources10
Helixi83 – 93Combined sources11
Beta strandi95 – 101Combined sources7
Helixi103 – 110Combined sources8
Beta strandi111 – 114Combined sources4
Helixi117 – 122Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D58X-ray1.90A17-123[»]
2G2BNMR-A2-147[»]
ProteinModelPortaliP55008.
SMRiP55008.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55008.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 80EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini81 – 115EF-hand 2; degeneratePROSITE-ProRule annotationAdd BLAST35

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410INJA. Eukaryota.
ENOG411206J. LUCA.
GeneTreeiENSGT00390000013846.
HOGENOMiHOG000231928.
HOVERGENiHBG004002.
InParanoidiP55008.
KOiK18617.
PhylomeDBiP55008.
TreeFamiTF320736.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P55008-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQTRDLQGG KAFGLLKAQQ EERLDEINKQ FLDDPKYSSD EDLPSKLEGF
60 70 80 90 100
KEKYMEFDLN GNGDIDIMSL KRMLEKLGVP KTHLELKKLI GEVSSGSGET
110 120 130 140
FSYPDFLRMM LGKRSAILKM ILMYEEKARE KEKPTGPPAK KAISELP
Length:147
Mass (Da):16,703
Last modified:October 1, 1996 - v1
Checksum:i0104CCF092074E8F
GO
Isoform 2 (identifier: P55008-2) [UniParc]FASTAAdd to basket
Also known as: G1

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.

Show »
Length:93
Mass (Da):10,455
Checksum:i4365FCB38F2B7329
GO
Isoform 3 (identifier: P55008-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: MSQTRDLQGG...EFDLNGNGDI → MEFDLNGNGD...PSPHPHPLPP
     121-147: Missing.

Show »
Length:132
Mass (Da):14,617
Checksum:iFC69F38ECC92A697
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33D → H in AAA92457 (PubMed:8081366).Curated1
Sequence conflicti131 – 147KEKPT…ISELP → RKTNTPPSQESPI in AAA92457 (PubMed:8081366).CuratedAdd BLAST17

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04866514G → R.2 PublicationsCorresponds to variant rs2736182dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0438371 – 65MSQTR…GNGDI → MEFDLNGNGDIGEKRVICGG RVVCRPKKTEVSPTCSIPHD LGGGPPTTVGGRRMGMRKWE RRERVSPPSPHPHPLPP in isoform 3. 2 PublicationsAdd BLAST65
Alternative sequenceiVSP_0438381 – 54Missing in isoform 2. 3 PublicationsAdd BLAST54
Alternative sequenceiVSP_043839121 – 147Missing in isoform 3. 2 PublicationsAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49392 mRNA. Translation: AAA92457.1.
U95213 mRNA. Translation: AAC24422.1.
U19713 mRNA. Translation: AAB05003.1.
Y14768 Genomic DNA. Translation: CAA75060.1.
Y14768 Genomic DNA. Translation: CAA75061.1.
Y14768 Genomic DNA. Translation: CAA75062.1.
D86438 mRNA. Translation: BAA13088.1.
AF299327 mRNA. Translation: AAG39110.1.
EF070982 mRNA. Translation: ABK35646.1.
AK290771 mRNA. Translation: BAF83460.1.
CR542153 mRNA. Translation: CAG46950.1.
AF129756 Genomic DNA. Translation: AAD18087.1.
BA000025 Genomic DNA. Translation: BAB63392.1.
AL662847 Genomic DNA. Translation: CAI17694.1.
AL805934 Genomic DNA. Translation: CAI18495.1.
AL662801 Genomic DNA. Translation: CAI18308.1.
AL805934 Genomic DNA. Translation: CAO03652.1.
CR753892 Genomic DNA. Translation: CAQ06958.1.
CR759761 Genomic DNA. Translation: CAQ10844.1.
BX248305 Genomic DNA. Translation: CAM26281.1.
CH471081 Genomic DNA. Translation: EAX03442.1.
CH471081 Genomic DNA. Translation: EAX03446.1.
BC009474 mRNA. Translation: AAH09474.1.
CCDSiCCDS34398.1. [P55008-2]
CCDS4706.1. [P55008-1]
PIRiJC5246.
RefSeqiNP_001305899.1. NM_001318970.1. [P55008-2]
NP_001614.3. NM_001623.4. [P55008-1]
NP_116573.1. NM_032955.2. [P55008-2]
XP_016865821.1. XM_017010332.1. [P55008-3]
UniGeneiHs.76364.

Genome annotation databases

EnsembliENST00000376049; ENSP00000365217; ENSG00000204472. [P55008-2]
ENST00000376059; ENSP00000365227; ENSG00000204472. [P55008-1]
ENST00000383473; ENSP00000372965; ENSG00000206428. [P55008-2]
ENST00000383474; ENSP00000372966; ENSG00000206428. [P55008-1]
ENST00000413349; ENSP00000416061; ENSG00000235985. [P55008-1]
ENST00000414149; ENSP00000391720; ENSG00000237727. [P55008-1]
ENST00000415830; ENSP00000413709; ENSG00000235985. [P55008-2]
ENST00000416422; ENSP00000399901; ENSG00000234836. [P55008-2]
ENST00000419376; ENSP00000401433; ENSG00000237727. [P55008-2]
ENST00000424944; ENSP00000399621; ENSG00000235588. [P55008-2]
ENST00000425748; ENSP00000398013; ENSG00000234836. [P55008-1]
ENST00000440907; ENSP00000397842; ENSG00000235588. [P55008-1]
GeneIDi199.
KEGGihsa:199.
UCSCiuc003nva.4. human. [P55008-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49392 mRNA. Translation: AAA92457.1.
U95213 mRNA. Translation: AAC24422.1.
U19713 mRNA. Translation: AAB05003.1.
Y14768 Genomic DNA. Translation: CAA75060.1.
Y14768 Genomic DNA. Translation: CAA75061.1.
Y14768 Genomic DNA. Translation: CAA75062.1.
D86438 mRNA. Translation: BAA13088.1.
AF299327 mRNA. Translation: AAG39110.1.
EF070982 mRNA. Translation: ABK35646.1.
AK290771 mRNA. Translation: BAF83460.1.
CR542153 mRNA. Translation: CAG46950.1.
AF129756 Genomic DNA. Translation: AAD18087.1.
BA000025 Genomic DNA. Translation: BAB63392.1.
AL662847 Genomic DNA. Translation: CAI17694.1.
AL805934 Genomic DNA. Translation: CAI18495.1.
AL662801 Genomic DNA. Translation: CAI18308.1.
AL805934 Genomic DNA. Translation: CAO03652.1.
CR753892 Genomic DNA. Translation: CAQ06958.1.
CR759761 Genomic DNA. Translation: CAQ10844.1.
BX248305 Genomic DNA. Translation: CAM26281.1.
CH471081 Genomic DNA. Translation: EAX03442.1.
CH471081 Genomic DNA. Translation: EAX03446.1.
BC009474 mRNA. Translation: AAH09474.1.
CCDSiCCDS34398.1. [P55008-2]
CCDS4706.1. [P55008-1]
PIRiJC5246.
RefSeqiNP_001305899.1. NM_001318970.1. [P55008-2]
NP_001614.3. NM_001623.4. [P55008-1]
NP_116573.1. NM_032955.2. [P55008-2]
XP_016865821.1. XM_017010332.1. [P55008-3]
UniGeneiHs.76364.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D58X-ray1.90A17-123[»]
2G2BNMR-A2-147[»]
ProteinModelPortaliP55008.
SMRiP55008.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106702. 1 interactor.
IntActiP55008. 2 interactors.
STRINGi9606.ENSP00000365227.

PTM databases

iPTMnetiP55008.
PhosphoSitePlusiP55008.

Polymorphism and mutation databases

BioMutaiAIF1.
DMDMi1703217.

Proteomic databases

EPDiP55008.
PaxDbiP55008.
PeptideAtlasiP55008.
PRIDEiP55008.
TopDownProteomicsiP55008-1. [P55008-1]

Protocols and materials databases

DNASUi199.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000376049; ENSP00000365217; ENSG00000204472. [P55008-2]
ENST00000376059; ENSP00000365227; ENSG00000204472. [P55008-1]
ENST00000383473; ENSP00000372965; ENSG00000206428. [P55008-2]
ENST00000383474; ENSP00000372966; ENSG00000206428. [P55008-1]
ENST00000413349; ENSP00000416061; ENSG00000235985. [P55008-1]
ENST00000414149; ENSP00000391720; ENSG00000237727. [P55008-1]
ENST00000415830; ENSP00000413709; ENSG00000235985. [P55008-2]
ENST00000416422; ENSP00000399901; ENSG00000234836. [P55008-2]
ENST00000419376; ENSP00000401433; ENSG00000237727. [P55008-2]
ENST00000424944; ENSP00000399621; ENSG00000235588. [P55008-2]
ENST00000425748; ENSP00000398013; ENSG00000234836. [P55008-1]
ENST00000440907; ENSP00000397842; ENSG00000235588. [P55008-1]
GeneIDi199.
KEGGihsa:199.
UCSCiuc003nva.4. human. [P55008-1]

Organism-specific databases

CTDi199.
DisGeNETi199.
GeneCardsiAIF1.
HGNCiHGNC:352. AIF1.
HPAiHPA049234.
MIMi601833. gene.
neXtProtiNX_P55008.
OpenTargetsiENSG00000204472.
ENSG00000206428.
ENSG00000234836.
ENSG00000235588.
ENSG00000235985.
ENSG00000237727.
PharmGKBiPA24646.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410INJA. Eukaryota.
ENOG411206J. LUCA.
GeneTreeiENSGT00390000013846.
HOGENOMiHOG000231928.
HOVERGENiHBG004002.
InParanoidiP55008.
KOiK18617.
PhylomeDBiP55008.
TreeFamiTF320736.

Miscellaneous databases

ChiTaRSiAIF1. human.
EvolutionaryTraceiP55008.
GeneWikiiAIF1.
GenomeRNAii199.
PROiP55008.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000204472.
CleanExiHS_AIF1.
ExpressionAtlasiP55008. baseline and differential.
GenevisibleiP55008. HS.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAIF1_HUMAN
AccessioniPrimary (citable) accession number: P55008
Secondary accession number(s): A8K406
, O43904, Q9UIV4, Q9UKS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.