P55005 (AMYB_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-amylase
EC=3.2.1.2

Alternative name(s):
1,4-alpha-D-glucan maltohydrolase

Gene names

Name:

BMY1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.
Sequence similarities	Belongs to the glycosyl hydrolase 14 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Molecular function	beta-amylase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Beta-amylase		PRO_0000153935
Regions
	Region	379 – 380	2	Substrate binding By similarity
Sites
	Active site	184	1	Proton donor By similarity
	Active site	378	1	Proton acceptor By similarity
	Binding site	51	1	Substrate By similarity
	Binding site	91	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	293	1	Substrate By similarity
	Binding site	298	1	Substrate By similarity
	Binding site	340	1	Substrate By similarity
	Binding site	418	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55005 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A04D57F1C73C064E	FASTA	488	55,180
	10 20 30 40 50 60 MAGNALANYV QVYVMLPLDV ITVDNTFEKE DETRAQLKKL TEAGADGVMI DVWWGLVEGK 70 80 90 100 110 120 EPGVYDWSAY RQVFKLVQEA GLKLQAIMSC HQCGGNVGDV VNIPIPQWVR DVGKSNPDIF 130 140 150 160 170 180 YTNRSGLTNI EYLTLGVDDQ PLFHGRTAIQ LYADYMKSFR ENMADFLDAG VVVDIEVGLG 190 200 210 220 230 240 PAGEMRYPSY PQSQGWVFPG VGEFICYDKY LQADFKAAAE EAGHPEWDLL DDAGTYNDTP 250 260 270 280 290 300 EKTQFFADNG TYQTDKGKFF LTWYSNKLIK HGDKILDEAN KVFLGCKVQL AIKVSGIHWW 310 320 330 340 350 360 YNVPNHAAEL TAGYYNLDDR DGYRTIAHML TRHRASMNFT CAEMRDSEQS SEAKSAPEEL 370 380 390 400 410 420 VQQVLSAGWR EGLNLACENA LNRYDATAYN TILRNARPQG INKNGPPEHK LHGFTYLRVS 430 440 450 460 470 480 DELFQEQNYT TFKTFVRRMH ANLDYNPNVD PVAPLERSKA EIPIEEILEV AQPKLEPFPF DKDTDLPV

References

[1]	"Characterization of a maize beta-amylase cDNA clone and its expression during seed germination." Wang S.M., Lue W.L., Wu S.Y., Huang H.W., Chen J. Plant Physiol. 113:403-409(1997) [PubMed: 9046591] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. TN351. Tissue: Aleurone.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z25871 mRNA. Translation: CAA81091.1.
PIR	S37075.
RefSeq	NP_001105496.1. NM_001112026.1.
UniGene	Zm.80.
3D structure databases
ProteinModelPortal	P55005.
SMR	P55005. Positions 7-488.
ModBase	Search...
Protein family/group databases
CAZy	GH14. Glycoside Hydrolase Family 14.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	542472.
KEGG	zma:542472.
Organism-specific databases
Gramene	P55005.
MaizeGDB	30053.
Family and domain databases
InterPro	IPR001554. Glyco_hydro_14. IPR018238. Glyco_hydro_14_CS. IPR001371. Glyco_hydro_14B_pln. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF01373. Glyco_hydro_14. 1 hit. [Graphical view]
PRINTS	PR00750. BETAAMYLASE. PR00842. GLHYDLASE14B.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00506. BETA_AMYLASE_1. 1 hit. PS00679. BETA_AMYLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYB_MAIZE

Accession

Primary (citable) accession number: P55005

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program