P55001 (MFAP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microfibrillar-associated protein 2
Short name=MFAP-2

Alternative name(s):
Microfibril-associated glycoprotein 1
Short name=MAGP
Short name=MAGP-1

Gene names

Name:	MFAP2
Synonyms:	MAGP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the elastin-associated microfibrils.
Subunit structure	Forms a ternary complex with BGN and ELN By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Post-translational modification	Forms intermolecular disulfide bonds either with other MAGP-1 molecules or with other components of the microfibrils. May form transglutaminase cross-links. O-glycosylated.
Sequence similarities	Belongs to the MFAP family. Contains 1 SXC (ShKT) domain.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
PTM	Disulfide bond Glycoprotein Pyrrolidone carboxylic acid Sulfation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	platelet formation Inferred from electronic annotation. Source: Compara
Cellular_component	microfibril Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P55001-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A' (identifier: P55001-2)

The sequence of this isoform differs from the canonical sequence as follows:
     13-13: Missing.

Isoform B (identifier: P55001-3)

The sequence of this isoform differs from the canonical sequence as follows:
     13-42: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Or 19 Potential
	Chain	18 – 183	166	Microfibrillar-associated protein 2		PRO_0000018682
Regions
	Domain	153 – 183	31	SXC
	Compositional bias	59 – 73	15	Gln/Glu-rich
Amino acid modifications
	Modified residue	18	1	Pyrrolidone carboxylic acid By similarity
	Modified residue	47	1	Sulfotyrosine Probable
	Modified residue	48	1	Sulfotyrosine Probable
	Modified residue	50	1	Sulfotyrosine Probable
	Disulfide bond	153 ↔ 183		By similarity
	Disulfide bond	160 ↔ 176		By similarity
	Disulfide bond	169 ↔ 180		By similarity
Natural variations
	Alternative sequence	13 – 42	30	Missing in isoform B.		VSP_042779
	Alternative sequence	13	1	Missing in isoform A'.		VSP_042780

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A75BE15DC9629B26	FASTA	183	20,826
	10 20 30 40 50 60 MRAAYLFLLF LPAGLLAQGQ YDLDPLPPFP DHVQYTHYSD QIDNPDYYDY QEVTPRPSEE 70 80 90 100 110 120 QFQFQSQQQV QQEVIPAPTP EPGNAELEPT EPGPLDCREE QYPCTRLYSI HRPCKQCLNE 130 140 150 160 170 180 VCFYSLRRVY VINKEICVRT VCAHEELLRA DLCRDKFSKC GVMASSGLCQ SVAASCARSC GSC
	Isoform A' [UniParc]. Checksum: F8179D36FF49C25B	FASTA	182	20,754
	10 20 30 40 50 60 MRAAYLFLLF LPGLLAQGQY DLDPLPPFPD HVQYTHYSDQ IDNPDYYDYQ EVTPRPSEEQ 70 80 90 100 110 120 FQFQSQQQVQ QEVIPAPTPE PGNAELEPTE PGPLDCREEQ YPCTRLYSIH RPCKQCLNEV 130 140 150 160 170 180 CFYSLRRVYV INKEICVRTV CAHEELLRAD LCRDKFSKCG VMASSGLCQS VAASCARSCG SC
	Isoform B [UniParc]. Checksum: 949A4852012F645A	FASTA	153	17,444
	10 20 30 40 50 60 MRAAYLFLLF LPDNPDYYDY QEVTPRPSEE QFQFQSQQQV QQEVIPAPTP EPGNAELEPT 70 80 90 100 110 120 EPGPLDCREE QYPCTRLYSI HRPCKQCLNE VCFYSLRRVY VINKEICVRT VCAHEELLRA 130 140 150 DLCRDKFSKC GVMASSGLCQ SVAASCARSC GSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the human gene for microfibril-associated glycoprotein (MFAP2), assignment to chromosome 1p36.1-p35, and linkage to D1S170." Faraco J., Bashir M.M., Rosenbloom J., Francke U. Genomics 25:630-637(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
[3]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A'). Tissue: Dermoid cancer.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Tissue: Uterus.
[7]	"The WashU-Merck EST project." Hillier L., Clark N., Dubuque T., Elliston K., Hawkins M., Holman M., Hultman M., Kucaba T., Le M., Lennon G., Marra M., Parsons J., Rifkin L., Rohlfing T., Soares M., Tan F., Trevaskis E., Waterston R. , Williamson A., Wohldmann P., Wilson R. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-99 (ISOFORM B).
[8]	"Revised genomic structure of the human MAGP1 gene and identification of alternate transcripts in human and mouse tissues." Segade F., Broekelmann T.J., Pierce R.A., Mecham R.P. Matrix Biol. 19:671-682(2000) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19718 mRNA. Translation: AAA79920.1. CR407678 mRNA. Translation: CAG28606.1. AK222751 mRNA. Translation: BAD96471.1. AL049569 Genomic DNA. Translation: CAB96824.1. AL049569 Genomic DNA. Translation: CAI20368.1. CH471134 Genomic DNA. Translation: EAW94821.1. BC015039 mRNA. Translation: AAH15039.1. H27217 mRNA. No translation available.
IPI	IPI00022621. IPI00644827.
PIR	I38923.
RefSeq	NP_001128719.1. NM_001135247.1. NP_001128720.1. NM_001135248.1. NP_002394.1. NM_002403.3. NP_059453.1. NM_017459.2.
UniGene	Hs.389137.
3D structure databases
ProteinModelPortal	P55001.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000364685.
PTM databases
PhosphoSite	P55001.
Polymorphism databases
DMDM	1708914.
Proteomic databases
PaxDb	P55001.
PRIDE	P55001.
Protocols and materials databases
DNASU	4237.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000375534; ENSP00000364684; ENSG00000117122. ENST00000375535; ENSP00000364685; ENSG00000117122. ENST00000438542; ENSP00000417029; ENSG00000117122.
GeneID	4237.
KEGG	hsa:4237.
UCSC	uc001azw.3. human.
Organism-specific databases
CTD	4237.
GeneCards	GC01M017300.
HGNC	HGNC:7033. MFAP2.
HPA	HPA007354.
MIM	156790. gene.
neXtProt	NX_P55001.
PharmGKB	PA30769.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG42345.
HOGENOM	HOG000231186.
HOVERGEN	HBG060871.
InParanoid	P55001.
OMA	MATSGLC.
OrthoDB	EOG4NGGNX.
PhylomeDB	P55001.
Enzyme and pathway databases
Reactome	REACT_118779. Extracellular matrix organization.
Gene expression databases
Bgee	P55001.
CleanEx	HS_MFAP2.
Genevestigator	P55001.
GermOnline	ENSG00000117122. Homo sapiens.
Family and domain databases
InterPro	IPR008673. MAGP. IPR003582. ShK_toxin. [Graphical view]
PANTHER	PTHR16485. PTHR16485. 1 hit.
Pfam	PF05507. MAGP. 1 hit. PF01549. ShK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	4237.
NextBio	16705.
SOURCE	Search...

Entry information

Entry name

MFAP2_HUMAN

Accession

Primary (citable) accession number: P55001
Secondary accession number(s): Q53X60, Q5JXY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.